isolation of murine adipose derived stem cells

... mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal stem cells ... system will provide a valuable tool, in addition to primary hepatocytes, for study of the molecular basis of the regenerative and developmental processes of hepatic cells in vitro Isolation of ... evidence of transdifferentiation by MET in the process of hepatic differentiation of AT-MSCs No previous report has demonstrated evidence of transdifferentiation of committed adult stem cells In...

Ngày tải lên: 30/03/2014, 04:20

... HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose- derived stem cells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal stem cells ... Immunophenotype of human adiposederived cells: temporal changes in stromal-associated and stem cell-associated markers Stem Cells 2006, 24, 376-385 26 Muir WW 3rd, Wiese AJ, March PA Effects of morphine, ... with isoflurane Am J Vet Res 2003, 64, 1155-1160 27 Ogawa R The importance of adipose- derived stem cells and vascularized tissue regeneration in the field of tissue transplantation Curr Stem Cell...

Ngày tải lên: 07/08/2014, 23:22

... there are two well defined types of stem cells – the embryonic stem cells and the adult stem cells The embryonic stem cells, which are found within inner cell mass of the embryonic blastocyst, is ... good source of multipotent adult stem cells is the adipose tissue Cells isolated from these, known as ADSCs (Adipose Derived Stromal Cells) have been shown to possess the property of being multipotent ... of stem cells and precursor cells for this purpose The two types of stem cells that are being targeted for use in tissue regeneration are the Embryonic stem cells and the Adult stem cells The...

Ngày tải lên: 08/11/2015, 17:01

... mesenchymal stem cells Stem Cells Dev 2005, 14:70-80 Aust L, Devlin B, Foster SJ, Halvorsen YD, Hicok K, du Laney T, Sen A, Willingmyre GD, Gimble JM: Yield of human adipose- derived adult stem cells ... cryopreserved human adipose- derived stem cells Cryobiology 2008, 57:18-24 Chen MY, Lie PC, Li ZL, Wei X: Endothelial differentiation of Wharton’s jelly -derived mesenchymal stem cells in comparison ... osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of protein by lipolytic hormones...

Ngày tải lên: 10/08/2014, 10:20

... characterization of other potential sources of stem cells which might avoid these issues altogether 1.1.2.1 Adult stem cells Stem cells are loosely defined as self-renewing progenitor cells that can ... use of stem cells in regeneration of bone 1-1 1-1 1-2 Chapter – Literature Review 2.1 Description of the adipose tissue 2.2 Adipose tissue – a possible alternative to bone marrow as a source of ... CHARACTERIZATION OF HUMAN ADIPOSE DERIVED ADULT MULTIPOTENT PRECURSOR CELLS LEONG TAI WEI DAVID (Bachelor of Chemical Engineering, Hons) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF...

Ngày tải lên: 12/09/2015, 09:55

... dilutions of DCs, from 10,000 cells per well to 313 cells per well, with × 105 allogeneic CD4 T cells in a 96-well U-bottomed plate in a total volume of 200 µl for days The proliferation of T cells ... function of DCs Polarization of naïve Th cells into Th1 cells is critical for the induction of cellular immunity against intracellular pathogens and cancer cells The observed impairment of the ... modulation of DC immunity by LV expression of different cytokines or siRNA molecules Materials and Methods Generation of monocyte -derived dendritic cells Peripheral blood mononuclear cells (PBMCs)...

Ngày tải lên: 13/08/2014, 13:20

... analysis and design SL performed the stem cell isolations from benign samples PB performed stem cell isolations from tumors CH performed culturing and isolation of stem cells, and NF-κB experiments JLL ... signature Stem cells Stem cells (c) Committed basal Committed basal Stem Committed cells basal (d) Malignancy signature Tumour Birnie et al R83.3 Gleason included Benign Figure Distinctive stem cell ... cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing concentrations of PTL Cells were...

Ngày tải lên: 14/08/2014, 08:21

... by the transfection The percentage of dead cells was calculated by expressing total number of PI-stained cells as a percentage of total number of DAPI-stained cells which were counted using Image ... Isolation of HPTC and characterization of isolated and commercial HPTC 50 4.1.1 Isolation of HPTC 50 4.1.2 Characterization of HPTC by immunoblotting 51 4.1.3 Characterization of ... by an asterisk (p-values of < 0.05) unless stated otherwise 49 Results 4.1 Isolation of HPTC and characterization of freshly isolated and commercial HPTC 4.1.1 Isolation of HPTC HPTC are available...

Ngày tải lên: 09/09/2015, 10:19

... 2.2 Stem cells in tissue regeneration 2.2.1 Embryonic stem cells 2.2.2 Adult stem cells 2.2.3 Adipose derived stem cells Chapter Chapter v 2.2.4 Hepatic differentiation of adipose derived stem ... of stemness marker genes on gelatin 49 microspheres 4.2.4 Adipogenic and osteogenic differentiation of 49 adipose derived stem cells 4.2.5 Hepatic differentiation of adipose derived stem 51 cells ... 3.2.2 Adipose derived stem cells isolation and culture 31 3.2.3 Cell seeding on gelatin microspheres 32 3.2.4 Total DNA quantification assay 32 3.2.5 Differetiation of adipose derived stem cells...

Ngày tải lên: 09/09/2015, 11:18

... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... Mesenchymal stem cell therapy in joint disease Novartis Found Symp 2003, 249:86-96 Zhang HN, Li L, Leng P, Wang YZ, Lv CY: Uninduced adipose- derived stem cells repair the defect of full-thickness ... with autologous adipose- tissue -derived stem cells: a case series Journal of Medical Case Reports 2011 5:296 Submit your next manuscript to BioMed Central and take full advantage of: • Convenient...

Ngày tải lên: 10/08/2014, 23:21

... G, Chen SH, Pan PY: Development and function of myeloid -derived suppressor cells generated from mouse embryonic and hematopoietic stem cells Stem Cells 2010, 28:620-632 Poschke I, Mougiakakos ... Characterization of cytokine-induced myeloid -derived suppressor cells from normal human peripheral blood mononuclear cells J Immunol 2010, 185:2273-2284 Kusmartsev S, Gabrilovich DI: Effect of tumor -derived ... shown from Suppression Assays of CD33+ or CD11b+ cells with autologous T cells, respectively * indicates statistically significant suppression of T cells by CD11b+ cells from co-culture (p

Ngày tải lên: 18/06/2014, 19:20

... may be derived from subsequent isolation and re-plating of the ICM cells, which are then screened for stemness by a variety of recognized markers Once in stable culture, ESCs are capable of either ... scoring system Stem cell research: social and political factors Public sharing of information about the basic science of ESCs has proven to be important, since those who are aware of the stem cell ... Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole embryos or compromising extant...

Ngày tải lên: 13/08/2014, 23:20

... the topic of this thesis 2.1 Embryonic stem cells Embryonic stem cells (ESCs) are pluripotent cells derived from the inner cell mass of the blastocyst Pluripotent diploid ESCs have been derived ... neonatal stem cells come in between embryonic and adult stem cells in the developmental continuum Studies of these cells have provided information and insights into the biology of stem cells in ... embryonic stem cells, induced pluripotent stem cells and then the adult bone marrow derived stem cells, which are well known and best studied for their immune modulatory properties Finally, cells...

Ngày tải lên: 11/09/2015, 10:02

... Vainzof M, Zatz M: SJL dystrophic mice express a significant amount of human muscle proteins following systemic delivery of human adipose- derived stromal cells without immunosuppression Stem Cells ... new potential source of pluripotent cells for regenerative medicine The identification of niches of tissue-specific stem cells capable of replacing damaged differentiated cells in the hFTs may ... Discussion The possibility of using stem cells for regenerative medicine has opened a new field of investigation to find the best sources for obtaining multipotent stem cells, in particular through...

Ngày tải lên: 18/06/2014, 15:20

... staining of IPFPderived cells It was therefore interesting that they showed a pattern of expression on a high proportion of the IPFP -derived cells and of epitopes commonly abundant on adult stem cells ... Clonal IPFP -derived cells retained the cell surface characteristics of the parent IPFP cells, which were similar to mesenchymal stem cells The clonal populations of IPFP -derived cells appeared ... characterisation of infrapatellar fat padderived cells The rate of proliferation of the IPFP -derived cells in monolayer culture was significantly increased by FGF-2 A comparison of their proliferation...

Ngày tải lên: 09/08/2014, 10:23

... retroviral vectors (that enables infection of a wide variety of cells) have revealed differences in the efficiency of single round infection in cells of differing types and species [11,12] Therefore, ... x 105 Figure cells with an HIV-EGFP vector at a moi = 0.5 Infection of parental and mutant HeLa cells (30-2 and 42-7) Infection of parental and mutant HeLa cells (30-2 and 42-7) cells with an ... parental and mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and...

Ngày tải lên: 13/08/2014, 05:22

... total amount of pravastatin entrapped in 0.1 ml of the final packed erythrocytes Efficiency of entrapment The percentage of the loaded amount of pravastatin to the total amount of that added ... percentage ratio of the hematocrite value of the final loaded cells to that of the initial packed cells, both measured using equal suspension volumes In vitro characterization of pravastatin loaded ... osmosis-based systems or by chemical perturbation of the erythrocytes membrane [16] Endocytosis is the process by which cells absorb molecules by engulfing them It is used by all cells of the body...

Ngày tải lên: 25/10/2012, 11:10

... media containing isoform I Both isoforms were also expressed intracellularly, as evidenced by the appearance of protein bands of the expected size upon SDS ⁄ PAGE analysis of the crude extracts ... recombinant isoforms Lane M, molecular mass standards production of the human recombinant isoforms might be enhanced by the presence of metal ions in the growth medium We found that addition of 200 ... the mean of three independent experiments and are presented as copies of the target variant per 109 copies of 18S RNA Quantitation of ACMSD variants in liver, kidney and brain An isoform-specific...

Ngày tải lên: 19/02/2014, 02:20