isolation and properties of an in vitro human outer blood retinal barrier model
Báo cáo y học: "Early Effects of anti-inflammatory [1, 2, 4]triazolo[4, 3-a] [1, 8]naphthyridine derivatives on human stimulated PMN and endothelial cells: an in vitro study" doc
... modulating their expression or intrinsic adhesive functions [18] PMNs maintain several of these receptors in an inactive state during transit in the bloodstream and extracellular fluids, and only ... Martini Hospital, Turin, for providing human umbilical cords, and the Blood Bank, Molinette Hospital, Turin, for providing human blood References 10 11 12 13 14 15 Vane JR, Bakhle YS, Botting ... 0.01) involved in development of the inflammatory process Most anti-inflammatory drugs exert their effects by inhibiting this pathway COX has been found in two isoforms, and COX-2 is an inducible...
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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx
... generate an in vitro library of VNAR domains, containing both synthetic and natural CDR3 loops, and then to isolate specific binding molecules using as an initial target antigen the mitochondrial outer ... whether binding affinity was retained in the absence of the conserved immunoglobulin intradomain disulphide bond In an initial series of experiments, recombinant 12F-11 protein was denatured and reduced ... (as indicated in Fig 1A) and different randomizations from Tom70 is an integral membrane protein consisting of a large globular receptor domain exposed to the cytosol and a short N-terminal transmembrane...
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Báo cáo y học: "Antiviral activity of Engystol® and Gripp-Heel®: an in-vitro assessment" pptx
... observe any induction of IFN-a in an epithelia and embryonic fibroblast cell line [7] As discussed below induction of IFN in cell lines is highly virus- and cell type dependent and might Figure In- vitro ... penicillin and 0.1 mg/ml streptomycin) Cells were incubated in serum-free MEM containing μg/ml trypsine, mM L-glutamine, 100 U/ml penicillin and 0.1 mg/ml streptomycin Determination of virus ... contributions BS and KR have substantially contributed to the interpretation of the data and were highly involved in drafting and revising the manuscript BS has given final approval of the version...
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báo cáo khoa học: " Involvement of S-adenosylmethionine-dependent halide/thiol methyltransferase (HTMT) in methyl halide emissions from agricultural plants: isolation and characterization of an HTMT-coding gene from Raphanus sativus (daikon radish)" ppt
... methyltransferase activity was observed in leaves than stems and roots in young seedlings and much weaker activity was found in mature plants in cabbage (B oleracea) On the other hand, AtHOL1 of A ... asetivum and B napus, and at 30°C and 133 μE/m2/s for O sativa, Z mays and Saccharum sp between and 15 days until enough leaves or blades were obtained Plant seeds (JP strains; Table 1) including ... and liquid standards of CH3I, DMS, and CH3SCN were prepared The detection limits of the GC-MS analysis for methyl halides, CH3SH, and DMS were around 0.03 ppm in the gaseous phase, and that of...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
... present (Stoveland and Lester, 1980; Lester, 1983) Various flocculating agents involving organic and inorganic compounds are widely used in wastewater treatment, dredging, and fermentation industries ... determined using the molybdenum-H2SO4 method (Schinttger et al., 1959) Protein was determined according to the method of Lowry et al (1951) using a standard of bovine serum albumin Nucleic acid and ... determined using the method of Blumenkrantz and Hansen (1973) with a glucuronic acid/galacturonic acid 1:1 standard Hexosamine was determined by the method of Blix (1948) with a glucosamine standard...
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Báo cáo Y học: Puri®cation and properties of an alkaline proteinase of Fusarium culmorum doc
... amount of contaminating trypsin-like proteinase caused an inactivation of the ÔsubtilisinÕ proteinase and, when that contaminant was inhibited by STI or BBI, the proteinase remained active STI and ... azogelatin Anal Biochem 263, 214±220 13 Bradford, M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal ... standard curve In all other cases the protein contents of the enzyme solutions were calculated by measuring their absorbance at 280 nm and assuming that mgámL)1 of protein had an absorbance of...
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Báo cáo sinh học: " Development of an in vitro cleavage assay system to examine vaccinia virus I7L cysteine proteinase activity" pptx
... Processing Kinetics of Core Protein Precursors To determine the optimal temperature and kinetics of processing of the core protein precursors in the in vitro cleavage assay, a time course of I7L-mediated ... reaction Effect of4 Figure inhibitors on in vitro processing Effect of inhibitors on in vitro processing Various concentrations of protease inhibitors were added to the in vitro processing assay for ... multiplicity of infection of plaque-forming units per cell and transfected with µg of plasmid DNA (either pI7L, or pI7LH241A) using DMRIEC (Invitrogen) following the manufacturer's indications Infected...
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báo cáo hóa học:" Development of an in vitro three dimensional loading-measurement system for long bone fixation under multiple loading conditions: a technical description" potx
... compression and bending load modes Published low strain rate longitudinal modulus of elasticity E determined by machine testing ranged from 17,000 to 22,600 (N/mm2) for human and bovine femur and tibia ... measured and stored along with the testing machine's ram position and applied load at sampling rates of 50 Hz axial, and 100 Hz torsion and bending NDI Toolbench software was used to determine the ... function of the longitudinal-circumferential shear modulus G, with published machine testing determined values ranging from 3300 to 5000 (N/mm2) for human and bovine femur and tibia [17] Thus use of...
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báo cáo hóa học:" Development of an in vitro cleavage assay system to examine vaccinia virus I7L cysteine proteinase activity" docx
... Processing Kinetics of Core Protein Precursors To determine the optimal temperature and kinetics of processing of the core protein precursors in the in vitro cleavage assay, a time course of I7L-mediated ... reaction Effect of4 Figure inhibitors on in vitro processing Effect of inhibitors on in vitro processing Various concentrations of protease inhibitors were added to the in vitro processing assay for ... multiplicity of infection of plaque-forming units per cell and transfected with µg of plasmid DNA (either pI7L, or pI7LH241A) using DMRIEC (Invitrogen) following the manufacturer's indications Infected...
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Báo cáo khoa học: "The conformation change of Bcl-2 is involved in arsenic trioxide-induced apoptosis and inhibition of proliferation in SGC7901 human gastric cancer cells" doc
... trioxide inhibits proliferation and induces apoptosis in SGC7901 human gastric cancer cells, accompanied by conformational changes of Bcl-2 and changes in total Bcl2 levels Methods Page of tured in ... cells were stained using DAPI staining B Untreated SGC7901 cells stained by anti-Bcl-2 N terminus antibody C SGC7901 cell stained by anti-Bcl-2 BH3 and anti-Bax(6A7) antibody before and after exposure ... recently gained increasing interest In general, over-expression and up-regulation of Bcl-2 has been associated with resistance to chemotherapy in various human cancers [29,32], and many studies...
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Báo cáo y học: "Isolation and characterization of microparticles in sputum from cystic fibrosis patients" pot
... antigens Anti -human CD11a labelling was used to numerate leukocyte MPs, while numeration of granulocyte MPs and monocyte/macrophage MPs was performed using anti -human CD66b and anti -human CD11b, ... Phenotype of MPs present in sputa of acute and stable CF and PCD patients In acute and stable CF patients, the number of MPs staining positive for FITC-conjugated antibodies directed against CD11a ... shown as mean ± SEM (Table 1) or medians (quantification and phenotype of MPs present in CF and PCD sputum) Statistical significance of differences between acute and stable groups of CF patients...
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Isolation and detection of bacteria in soil, ash and sediment with the contamination of paraquat and glyphosate
... in Very fine gravel Granule to -1 1–2 mm 0.039–0.079 in Very coarse sand to 0.5–1 mm 0.020–0.039 in Coarse sand to 0.25–0.5 mm 0.010–0.020 in Medium sand to 125–250 µm 0.0049–0.010 in Fine sand ... their root Animals and humans eat those plants, they can be impacted by contamination And then humans may be affected, when they ingest the contaminated animals, or expose to contaminated soil ... discarded, and the remaining supernatant was added more into the Spin Filter and continues to be centrifuged The next step was that 300 µL of solution MD4 was add more into the supernatant and also...
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Báo cáo y học: "CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model" pdf
... five membrane-spanning segments of CD47/IAP and the two membrane-spanning domains of the heterodimeric integrin form an ad hoc seven-transmembrane receptor that, with an adhesive ligand such as TSP, ... TSP-1, SIRPα, and α5 integrin TSP-1 and α5 integrin, but not SIRPα, were shown to immunoprecipitate with CD47/IAP (Figure 3b) Immunoprecipitation of α5 integrin and β1 integrin and subsequent ... cell line, rather than human chondrocytes, in an attempt to provide additional understanding of CD47/IAP involvement in cellular mechanotransduction Although these cells may express integrin profiles...
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Study of human nasal epithelial stem or progenitor cell growth and differentiation in an in vitro system
... nasal mucosa and NP biopsies, and the implication of this cell model in the study of the pathogenesis and molecular mechanisms leading to aberrant epithelial remodeling and impairment of mucociliary ... changes of NP in vitro In conclusion, we established an in vitro model for validating and comparing hNESPCs morphology and functional activity in the cells from NP and healthy controls, and demonstrated ... intraflagellar transport also Tg737, intraflagellar transport 88 intraflagellar transport protein A intraflagellar transport protein B immunohistochemical staining interleukins interleukins 13 interleukin...
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Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
... endocytosis and shape changes in human red blood cells and ghosts Blood 1986; 68:1008-1014 29 Schrier SL, Junga I, Ma L Requirements of drug-induced endocytosis by intact human erythrocytes Blood Cells ... effect of concentration and incubation time on the percent of drug loading is shown in figures and at 25ºC and at 37ºC The percent is of drug loading was started from 15% after 15 minutes upon using ... membrane[36] and also the loading procedure does not affect the MCV This finding is in agreement with previous report[34] Non-significant change in both MCH and MCHC can be explained by pravastatin...
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Báo cáo khoa học: Effect of mutations in the b5–b7 loop on the structure and properties of human small heat shock protein HSP22 (HspB8, H11) pptx
... b-strands are numbered and the b5 and b7 strands are shown in red and blue, respectively G104 (equivalent to K137 of human HSP22) and R108 (equivalent to K141 of human HSP22) are shown in purple and ... Dashed blue and red lines above human aB-crystallin and below human HSP27 sequences indicate a-helices and b-strands predicted in the models of the corresponding proteins [16,18] Residues of HSP22 ... HSP22 and its mutants, we plotted ln(At ⁄ Ao) (where Ao and At are the intensities of the band of intact protein at the beginning of trypsinolysis and at the fixed time of trypsinolysis) against...
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báo cáo hóa học:" Fibrin and poly(lactic-co-glycolic acid) hybrid scaffold promotes early chondrogenesis of articular chondrocytes: an in vitro study" docx
... type II and aggrecan core protein was steadily expressed in fibrin/PLGA and PLGA Interestingly, suppression of collagen type I was observed in fibrin/PLGA and PLGA at weeks and weeks β-actin gene ... presence of antibiotics and antimycotic (Gibco), 200 mM L-glutamine (Gibco) and 50 μg/ml of ascorbic acid (Sigma) All cultures were maintained in 5% CO2 incubator (Optima Model 560, Optima Inc, USA) ... that fibrin would be an ideal cell carrier/transplantation matrix and to enhance in vitro chondrogenesis of rabbit articular chondrocytes by mean of morphological, histological, biochemical and phenotypically...
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Báo cáo y học: "An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue" pot
... immunofluorescence and 3,3-diaminobenzidine disclosure) for aggrecan and the staining intensity increased with time post injection (Figures and 5b) Aggrecan matrix staining within the vicinity of ... expression and localisation of the chondrogenic transcription factor Sox-9, and of the matrix genes aggrecan and types I, II and X collagen within MSCs both in the monolayer and in those injected into ... X collagen and Alizarin red staining, and participated in interpretation of the data AJF participated in interpretation of the data and contributed to the preparation of the final manuscript JAH...
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Báo cáo y học: "Differential direct effects of cyclo-oxygenase-1/2 inhibition on proteoglycan turnover of human osteoarthritic cartilage: an in vitro study" pptx
... this and our findings, we assume that aceclofenac has comparable selectivity The metabolism of aceclofenac differs from that of diclofenac and is human specific [34] The main metabolite of aceclofenac ... participated in its design and coordination, and helped to draft the manuscript SM and NJ carried out the experiments and performed all of the assays All authors read and approved the final manuscript ... quiescence, and induces apoptosis in HT-29 colon adenocarcinoma cells J Clin Invest 1995, 96:491-503 47 Zhang X, Morham SG, Langenbach R, Young DA: Malignant transformation and antineoplastic actions of...
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Báo cáo y học: "Gender differences and inflammation: an in vitro model of blood cells stimulation in prepubescent children" pptx
... can depend on the type and intensity of the stimulus, and vary according to the considered cytokine LPS induced similar levels of IL1 production in males and females, with levels increasing in ... CH, FC, JD and NL participated in the design of the study GC and JD conceived of the study, and participated in its design and coordination All authors read and approved the final manuscript Author ... doses of LPS and Pokeweed Mitogen lectin as stimulants in vitro LPS-induced signalling in macrophages, and in other LPS-responsive cells such as neutrophils, is known to be initiated by interaction...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25