... positive for FSP and negative for cytokeratin 20 in all the cultured and examined cells (Figure 3) Discussion Q-banding analysis of FBs from CD and non-CD subjects demonstrated a normal and stable ... endotoxin and exert cytotoxic effects on primary cultures with an increase of lipidic intracellular droplets [29] Liberase is a blend of highly purified enzymes used to improve the isolationand cultures ... majority of affected ones The method of cell culture here described could help in the establishment of novel experiments to study the role of FBs in the pathogenesis of the mucosal damage and to...
... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular...
... for cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of oligo(dT) primer and 10 mm of dNTPs were incubated for at 65 °C Subsequently, lL of the first strand buffer X and lL of dithiothreitol ... mixture of other isoforms (B) Gel visualization of proteins eluted Lane 1, molecular weight markers Numbers on the left indicate the Mr values of the standards in thousands Lanes 2–9, lg of peak ... P III ⁄ P IV and a mixture of other isoforms (*), respectively, in the presence (lanes 2–5) or absence (lanes 6–9) of 2-mercaptoethanol (C) Elution profile of P III and P IV Chromatofocusing chromatography...
... of 50 nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and ... of 0.6 lM The data were fitted to Michaelis–Menten curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of ... Asterisks and broken line: data and fitting with PHI/PHO ratio of hypothesis and therefore a direct involvement of PHI in electron transfer This conclusion is consistent with the very low degree of identity...
... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for ... between NrfH and NrfA is discussed The reassessment of previous spectroscopic studies was undertaken, particularly regarding the assignment of spectroscopic and redox potentials of the NrfA hemes...
... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) from the essential oil (300 mg) in 2.4 mL of the ... the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ether/acetonitrile/acetone 2:1:1 and 4:3:1 v/v/v) are 0.34 and 0.24, and 0.20 and 0.20, respectively, which ... Both of the upper and the lower phases were degassed for 30 by ultrasonication before use Aliquots of 300 mg of the essential oil were diluted by 2.4 mL of the mixed solution of lower phase and...
... BLAST of peptide sequences of 21 kDa proteins in databases Tryptic peptides were puri®ed by RP-HPLC and some of them were totally or partially analyzed by Edman sequencing (ááá) and/ or by MALDI-TOF ... BAS1 protein of Brassica, spinach, barley and A thaliana, PR1 of Phaseolus and MHF of A thaliana These proteins belong to the 2Cys-Prx subfamily All plant 2Cys-Prx proteins, except BAS1 of barley, ... previously [21] Chloroplastic thioredoxins m and f from spinach were a kind gift of P Schurmann (University È of Neuchatel, Switzerland) Thioredoxins m and h from à Chlamydomonas were puri®ed as...
... presequence of 19 amino acids The mature protein would have a molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and the d-CDes from Arabidopsis showed an overall identity of 36% and ... the presence of d-cysteine in the culture medium It was suggested that d-cysteine exerts its toxicity through an inhibition of threonine deaminase On the other hand, the presence of the yedO gene ... d-CDes mRNA and protein levels in older plants, during the light phase in a diurnal light ⁄ dark rhythm and under sulfate limitation Results In silico characterization andisolationof the Arabidopsis...
... respectively, and the resultant VNARs evaluated for retention of binding affinity Material and methods Equipment and reagents Restriction enzymes and Vent DNA polymerase were purchased from New England ... possibility of inter- and intra CDR disulphide cross-links These strategies are summarized in Fig 1B and Table 1, and details of the library construction are given in greater detail in the Materials and ... possibility of disulphide bond formation genetically by replacement of residues Cys22 and Cys82 with alanine and valine, respectively, to give a cysteine minus mutant (12F-11DCys) Use of alanine and...
... permissive temperatures of 30 °C and 37 °C, respectively A sample of the cells was removed from each culture, rRNAs were isolated in TRIzolÒ (Invitrogen, Paisley, Scotland), and the extent to which ... site molecules Y80, Y123 and E177 are shown in green and the position of the two mutated amino acids, F108 and L151, are shown in blue between the aromatic rings of Tyr80 and Tyr123 close to the ... diagram of wild-type RTA structure, and the positions of the altered amino acids of the double mutant, F108 and L151, within the structure are indicated Discussion RTA is the catalytic polypeptide of...
... Switzerland), thermolysin, chymotrypsin or elastase (Sigma, St Louis, MO, USA)] and Cloning of the bovine MUC15 cDNA by PCR with degenerate primers Isolationof total RNA from the mammary gland of ... PCR screening of bovine and human cDNA libraries showed the presence of MUC15 mRNA in human breast, bovine mammary gland, bovine lymph nodes and lungs of both species (Table 1) Of the identified ... part of the coding sequence was then cloned and sequenced using PCR as described above with 0.4 lM and lM of the specific P7 primer and degenerate primers (P4-P6), respectively The cDNA sequence of...
... Asi iN -U 橋 d h ensa oH-O -AYU 篭車 一 F-u Uk ル KU ー K-s Utu b-U uS cuUk h a n E anD? k-S uTU B-u Us CUu H - ・ka N e And? 月 R 靴 ぶす rn a I cny ad big rde cosik hptcs dg o tI rn a g o mrig o d onn ... a δi , l s ocsoal,o h u o t i rvr w e h dos o of r o h c r w o ie y u hne f o l e h n e rp y u f o t t l k h gvs o cag.Iy u i e e v rt i ae i Koof g aot aig o o r rgt otd o Jpns c s s n yt ,o ... te ci n a s ses ts i Nvrhls,ery vr aaya ad fie ay n s k ad eetees nal eeyslrmn n ofc ld i O a a n truhu asihsland osek tnad aaee o ue n hogotKna a ere t pa sadr Jpns f s i r bsns,lhuh e r rnnito...
... of degenerate and specific primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... upper strands with [32P]ATP[cP] and T4 polynucleotide kinase The labeled oligomers were annealed by heating to 90 °C an equimolar mixture of the upper and lower strands in reaction buffer and cooling ... -2 and -3) The Xenopus XHIVEPs are characterized with respect to temporal and spatial expression, nuclear import/export activity and DNA binding specificity Materials and methods Isolation and...
... (Laumann and Derick, 2006) The movement of tattooing into the realm of popular culture displays certain features of the contemporary culture industry and reveals how fad-like phenomena emerge Culture ... art, prison art, tiki art, anime and manga, pulp art, and tattooing Inspired by the dadaists and surrealists of the 1920s and 1930s, advocates and practitioners of lowbrow art reject the constraints ... provide an account of the changes that have occurred within the artistic and cultural world of tattooing and the larger world of body alteration in the past decade and a half One change and a fairly...
... University of Kuopio, Finland The experiments were conducted according to the Council of Europe (Directive 86/609) and Finnish guidelines, and approved by the State Provincial Office of Eastern Finland ... behaviour of microglia in different culture models has been shown to be affected by the culture time and the composition ofculture media [12-14] It has been emphasized that the presence of serum ... serum-free culture conditions to examine the behaviour of microglia per se and to investigate how these slices respond to pro- and anti-inflammatory stimuli This in vitro cultureof postnatal...
... Domains of E9 and locations of mutations responsible for drug resistance Domains of E9 and locations of mutations responsible for drug resistance The location of the putative exonuclease and polymerase ... streptomycin and non-essential amino acids Vero Cells were obtained from ATCC and were maintained in MEM with Earl's salts and the addition of 10% FBS and standard concentrations of L-glutamine, ... Mapping and marker rescue of recombinant viruses A Map of PCR fragments in the E9L region that were used for marker rescue mapping experiments and reconstruction of resistant viruses B Results of...
... the G and P serotype specificities of the YK-1 strain, the sequences of the genes encoding both VP7 and VP4 were determined and compared with those of representatives of established G and P serotypes ... infectivity, immunity, and pathogenesis of rotavirus and for evaluating candidate rotavirus vaccines [16,17] Results Rotavirus isolationand characterization High titers of rotavirus antigen measured ... rotavirus RNA segment 11, and (c) schematic diagram of the sequences of gene segment 11 Arrows indicate segment 11 of RRV and YK-1, or rearranged segment 11 of variant 311 The YK-1 and its variant clone...
... strains), C1-6 (strains of Cornell Univ.) and P1-7 (strains from E coli reference center of Pennsylvania State Univ.) b The presence of Stx1 and Stx2 “-” and “+” indicate negative and positive, respectively ... determination of stx1, stx2, eaeA, and hlyA in multiplex PCR assay, (iii) to compare the genetic patterns of Korean isolates and U.S isolates, and (iv) to compare the efficiency among conventional culture ... Switzerland) Once suspicious colonies are identified, confirmation of the isolates as E coli O157:H7 is dependent upon biochemical identification and demonstration of the presence of somatic and...
... conditions and basic settings for the application of gene delivery were also investigated Materials and Methods Cell isolationandculture Canine umbilical cord blood (cUCB) and blood of the canine ... in the bottom of the tube (Fig 3D), and positive to toluidine blue staining (Figs 4E and F) Discussion Isolationand characterization of stem cells derived from various tissues and sources have ... the cell number The CPDL was measured and calculated and drawn as a graph A Fig Identification of the cumulative population doubling level (CPDL) andcultureof canine umbilical cord blood (cUCB)-mesenchymal...