isolation and culture of human multipotent stromal cells from the pancreas
Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection" pdf
... and mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and growth ... respectively These drugs select against the expression of the HPRT and APRT gene products since expression of these proteins results in the incorporation of the toxic purine analogues into DNA The genes ... in the amount of DNA integrated into the genome of mutant cells Clones 30-2 and 42-7 are resistant to MLV and HIV-1 infection We then quantified the resistance to infection of clones 30-2 and...
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báo cáo hóa học:" Isolation and culture of fibroblasts from endoscopic duodenal biopsies of celiac patients" pdf
... represent the majority of affected ones The method of cell culture here described could help in the establishment of novel experiments to study the role of FBs in the pathogenesis of the mucosal ... management of the endoscope and endoscopic forceps, and their passage through the endoscopic channel together with the patients' gastric juice and saliva For these reasons we used a higher dose of antibiotics ... FBs from endoscopic duodenal biopsies of CD and non-CD subjects, to analyze the growth patterns of the cultures and to compare the basic morphometric characteristics of FBs Methods Patients From...
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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... Consequently, the molecular studies of the interaction of SAgs with their specifics ligands will not only advance understanding of the physiological mechanisms of these molecules, but may lead to the development ... expansion of T -cells bearing human Vb3, Vb12, Vb17 and Vb19 [20] Others showed proliferation of human T -cells bearing Vb1, Vb2, Vb3, Vb5.2, Vb12, Vb15 and Vb17 domains and found differences in the ... species; reduction and alkylation, and mutation of the Cys implicated in the intermolecular disulfide bridge Analysis of the three-dimensional structure of SSA [31] to determine which of the remaining...
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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc
... isolated and sequenced the gene encoding the 21-kDa polypeptide Degenerate oligonucleotides designed from the sequences of N- and C-terminal peptides (P1 and P10) were used as primers to synthesize the ... X97910 The missing amino-acid stretches and the transit peptide sequence were deduced from the cloned cDNA and gene sequences (accession numbers: AJ304856 for the gene and AJ304857 for the cDNA) The ... the results support the notion that the redox state and/ or the concentration of reactive oxygen species in the chloroplast play a role in regulating the level of transcripts of the Ch-Prx1 gene...
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Báo cáo khoa học: " Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)" ppt
... bodies of GPV in the cytoplasm of cells Scale bars = 200 nm Isolation and identification of a canine coronavirus strain from giant pandas 263 isolated The electron microscopy results provided further ... TCID50 of the virus to each well The neutralization titer is reported as a logarithm of the highest dilution of serum that could neutralize 100 TCID50 of the virus Total RNA was extracted from the ... 3A, the amino acid sequence of the amplified GPV S gene was 98.7% identical to the S protein of CCV K378 In addition, the sequence of the GPV S gene was also 84.3% to 100% identical to the other...
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báo cáo khoa học: " Involvement of S-adenosylmethionine-dependent halide/thiol methyltransferase (HTMT) in methyl halide emissions from agricultural plants: isolation and characterization of an HTMT-coding gene from Raphanus sativus (daikon radish)" ppt
... It is therefore important to evaluate the level of emissions of volatile compounds, such as methyl halides, from these plants and to clarify the mechanism of synthesis of these compounds The emission ... encoded HTMT from R sativus HT performed the isolation and heterologous expression of the HTMT encoding gene from R sativus and characterization of the enzymatic properties of recom- Page of 10 (page ... emissions reflected those of the tested plants alone, and were not affected by the presence of soil microorganisms The results of the present study, together with the report of an HMT homologue in...
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Báo cáo khoa học: "Isolation and characterization of Treponema phagedenis-like spirochetes from digital dermatitis lesions in Swedish dairy cattle" pps
... lesions of DD were reproduced in any of the animals in the challenge trial Isolates T 603 and T 657 are from the same herd that is geographically remote from the other herds in the study They have ... susceptibility of the Treponema spp from cattle with DD The objectives of this study were to obtain pure cultures of Treponema spp from DD lesions in Swedish dairy cattle and to subject the cultures ... carried out the RAPD analysis and contributed to the evaluation of the results HH and MP adapted the PFGE protocol for the phylotype studied CB, HH, and KEJ participated in planning of the investigation...
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Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... 72% and 85% inhibition of BHLmL2 and BHAb, respectively, while mm 2-mer- captoethanol caused 85% and 97% inhibition of BHLmL2 and BHAb, respectively The influence of pH on the activities of the ... resulted in 55% and 51% loss of the initial activities of BHLmL2 and BHAb, respectively These results strongly suggest that Ser is involved in the catalytic activity of BHLmL2 and BHAb and are consistent ... liquid cultures were induced with 3-phenylindole (0.20 mm), and the cultures were incubated for an additional 24 h and then gravity filtered to separate the mycelia from the broth For analysis of...
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A guide to larvae and juveniles of some common fsh species from the Mekong River Basin
... covers the period from fertilisation to hatching of the egg During incubation, the embryo cannot feed, but is nourished by the egg yolk and other food stores The embryo’s cells divide and differentiate ... development of aquaculture and in many other applied research fields The Mekong is a regional hotspot for biodiversity, and several of the fish featured in this guide are listed on the IUCN Red List of ... recorded from the Mekong basin, and about two thirds of these (including most of the common species) are from purely freshwater families (Hortle, 2009), so there is a very large gap in the information...
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Báo cáo khoa học: Identification and characterization of novel salivary thrombin inhibitors from the ixodidae tick, Haemaphysalis longicornis doc
... inhibit activation of factors V and VIII by thrombin and then prevent these cofactors from forming prothrombinase and tenase complexes On the other hand, as shown in Fig 7, madanin and suppressed ... madanin and specifically interacted Fig Effect of madanin and on prolongation of APTT and PT The effects of madanin and on intrinsic and extrinsic pathways were investigated by APTT (A) and PT (B) ... dissociation of the complex was very fast and the reaction between ligand and analyte equilibrated very rapidly As the association and dissociation phases in the interactions of madanin and with thrombin...
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Báo cáo y học: "dentification of blood biomarkers of rheumatoid arthritis by transcript profiling of peripheral blood mononuclear cells from the rat collagen-induced arthritis model" doc
... the largest, and let P(i) be the ith sorted P value and i its rank Then, the FDR for each sorted P value was calculated by timing the P value with m/i, and monotonizing all of the FDRs from the ... destruction of the extracellular matrix, and eventual damage to the affected joints [5,6] Although there have been many studies of cells within the arthritic joint, the responses of the peripheral ... and members of the functional genomics group for helpful discussion, and the members of the Shou laboratory for critical reading of the manuscript We also acknowledge the support from the bioinformatics/IT...
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Báo cáo Y học: Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding pot
... may affect the heparin-binding properties of HCII Here, we report on the structure of posttranslational modifications of plasma-derived and recombinant human HCII from CHO cells and their effects ... similarity of HCII from human serum and its recombinant counterpart from CHO cells with respect to tyrosine sulfation and N-glycosylation The remarkable identity of oligosaccharide antennarity and the ... (GSKGPLDQLEKGGE), irrespective of whether HCII had been isolated from plasma or from recombinant CHO cells Thus, these results are in agreement with the accurate and efficient cleavage of the 19-amino-acid...
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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx
... Analysis of the binding of the Fab fragment of monoclonal antibody NC10 to influenza virus N9 neuraminidase from tern and whale using the BIAcore biosensor: effect of immobilization level and flow ... alignments and for CDRs and In these cases extensive loop modelling was undertaken and the final model selection based on the modeller objective score Construction of the 12F-11DCys mutant and 12F-11 ... background, and all of these proved to be identical sequences One of these, designated clone 12F-11, was chosen for further analysis The primary and deduced amino acid sequences of clone 12F-11...
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Báo cáo khoa học: Structure and function of human a-lactalbumin made lethal to tumor cells (HAMLET)-type complexes pptx
... by tumor cells from 30 to h HAMLET is initially bound to the membrane of the cells and subsequently transported into the cells The cells maintain cellular integrity for a long period of time (180 ... from the one-dimensional H NMR spectrum of ELOA, showing up-field shifts of the resonance of bound oleic acid compared with those of free molecules The 1H NOESY spectrum of ELOA demonstrated the ... radii of HAMLET (Rh = 26.9 A), which is intermediate of the hydrodynamic radii of the acidic molten globule state of a-lactalbumin ˚ (Rh = 20.9 A) and the theoretically extreme expansion state of...
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Báo cáo khoa học: MicroRNA-23b mediates urokinase and c-met downmodulation and a decreased migration of human hepatocellular carcinoma cells doc
... in pixels The expression percentages were the sum of the IODs of the bands corresponding to the precursor (170 kDa) and the b-chain (145 kDa) forms of c-met protein [19] The amount of target ... SKHep1C3 cells can be modulated by expression of miR-23b On the other hand, the anti-miR-induced inactivation of miR23B in AB2 fibroblasts did not modulate the migration ability of these normal cells ... in these tumor cells and in normal cell lines, respectively increasing or decreasing the stability of uPA mRNA and therefore impairing or favoring their degradation As the uPA mRNA of certain human...
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báo cáo hóa học:" Highly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturation" docx
... cell line These cells have been shown to express the human leukocyte antigen-DR (HLA-DR), the IL3-receptor (CD123) and the CD4 [18] Moreover, as a hallmark of pDC, these cells are BDCA2 and BDCA4 ... representative CFSE profile at the ratio 3/1 for GFP+ cells The data are shown as the means of independent experiments duced the GEN2.2 cells, no significant modification of the CD123 and HLA-DR expression ... the loss of CFSE fluorescence The data in panel A are shown as the mean of triplicate and represent one out of independent experiments whereas the data in panel B were performed once and synthetic...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture ... H9 and BG01v are names of hES cells lines P denotes the number of passages of the cell lines H9-EB denotes embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture ... (B) Figure Sequence and GO analysis of the miR-302 cluster and miR-520 cluster Sequence and GO analysis of the miR-302 cluster and miR-520 cluster The members of the miR-302 and miR-520 clusters...
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Báo cáo sinh học: " Transmission of human hepatitis C virus from patients in secondary cells for long term culture" pot
... possibilities The measured level of the negative strand in the cells remained stable Since the number of cells and the percentage of cells that are infected don't change during the culturing of the virus, ... that the blood of a patient and the isolate in culture are both of type 1b There were no significant differences in the sequences of the patient and the isolate in this region (Revie, Alberti, and ... Isolation1 of HCV from human patients Isolation of HCV from human patients (A) Isolation scheme for the replication of HCV in vitro (B) History of transmission of the specimen donated from HCV infected...
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báo cáo hóa học: " Comparing the immunosuppressive potency of naïve marrow stromal cells and Notch-transfected marrow stromal cells" docx
... priming and activating naïve and memory T cells and analyzed on the BD FACS Calibur using CellQuestPro Page of 14 Ex vivo culture of human peripheral blood T cells Human T cells were enriched from ... underlying mechanism of action To assess the impact of SB623 cells on the acquired immune cells, we performed co-cultures of human enriched T cells with SB623 cells or MSCs and assessed the activated ... in the allogeneic MLR, validating the functionality of the assay In the presence of SB623 cells, the percentage of CD4+ helper T cells expressing CD69 was reduced and this was comparable to the...
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báo cáo hóa học:" Effects of TGF-β1 and IGF-1 on proliferation of human nucleus pulposus cells in medium with different serum concentrations" pptx
... tissue-engineering and gene therapy techniques could offer a sustained release of growth factors, and the results from the present study could be used in the regeneration of the lost functional capacity of the ... and MTT assay, participated in the acquisition of data and drafted the manuscript Z Chao participated in the design of the study and performed the statistical analysis D.K Ruan conceived of the ... The morphology of human NP cells under 10%FBS conditions The morphology of human NP cells under 10%FBS conditions 10%FBS, 100 μg/L IGF-1, the morphology of NP cells were similar to the 10 μg/L TGF-β1...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25