... Sequencing of individual bands Sequencing of clones Southern Hybridization analysis Genetic Fingerprints Adaptation of culture conditions Hybridization analysis Fluorescent insitu hybridization (FISH) ... the vertical distribution of NH4+- and NO2 oxidizing bacteria in the corresponding biofilm determined by use of FISH technique (Fig.3A) We also analyzed the spatial distribution of ammonia- and ... between the insitu activity of NH4+ and NO2- oxidation and the spatial distributions of NH4+- and NO2 oxidizing bacteria within biofilms grown in different media This is a good example showing a correlation...
... believe in professionalism and perseverance We power a high performance organization We believe in sustainability and longterm thinking For us, it is not about reaching limits, it is about rising ... flaw in a process, it is her/his duty to improve it until it is solved All of us work hard to be part of the solution, not of the problem No ranks, no titles – whoever is working on solving a ... •Seek cooperation •Start by letting loose of individual goals •Concentrate on individual motives •Exchange interests and develop common goals by creating an understanding for the other partys...
... This period was marked by the optimism so characteristic of all humanist literature , the sky of his mind and his heart is cloudless His view point towards man (woman), life is very optimistic ... thinkers of Renaissance placed their interest in humanbeings They agreed with the ancient thinkers in evaluating and exalting the dignity of humanbeings They considered men to be the creator of ... the optimistic atmosphere of the play and especially it is the work written in the first period, it reflects his point of view in this period which is very optimistic ,so it is ranked in comedy...
... activated in the early phase of adipogenesis [15] The expression of antisense COX-2 mRNA results in the upregulation of adipogenesis, thus indicating that COX-2 is involved in the production of anti-adipogenic ... mechanism of this Fluprostenol-mediated increase in PGF2a production in adipocytes, at first we investigated the expression of genes involved in the biosynthesis of PGF2a and FP receptor in Fluprostenol-treated ... expression of COX-2 During the adipogenesis of mouse 3T3-L1 cells, PGF2a is produced in the early phase of adipogenesis with a peak at h after the initiation of adipogenesis [15] In this study, we found...
... fluorescent staining of cultured cells and tissues is presented thereby allowing investigators to utilize the TUNEL staining procedure with a minimal investment in laboratory reagents and equipment In addition, ... to avoid trapping air bubbles which may lead to staining artifacts Stop reaction by incubating slides × 10 in 2×SSC Rinse slides in PBS then block nonspecific binding by covering tissue sections ... desired level of staining is achieved (typically 10–60 min) Stop the reaction by incubating slides in DDW 12 Lightly counter-stain tissue sections with hematoxylin stain 13 Cover tissue sections...
... questions regarding phase explosion induced by picosecond laser pulses, since a finite amount of time is required for the formation of an equilibrium distribution of homogeneous nuclei in the superheated ... fraction of the light to a wavelength of 532 nm Any light remaining at the fundamental wavelength is removed in an infrared absorbing filter The probe beam then travels through a retroreflecting prism ... with the diameter of the attenuated region increasing to approximately 81 mm at 50 ps A distinct, sharp boundary to this region is visible after 140 ps The level of attenuation again decreases very...
... micro-organisms are let to inoculate for several hours before counting Considering that counting large numbers of cells is troublesome, the number of colony forming units (CFU) is determined instead of ... contribution of UV radiation to the inactivation process is not important, when not at all relevant By comparing www.intechopen.com Inactivationof Bacteria by Non-Thermal Plasmas 31 the killing kinetics ... improvement of current treatment methods is a major issue in dentistry (Sladek et al., 2004) Preparation of cavities prior to filling is done by removing necrotic, infected and non-remineralizable tissue...
... range, are playing the main role in the inactivation process Finally, the measurements of the UV intensity show that the UV emission is too weak to play a crucial direct role in the inactivation ... electrode is inserted into a hollow barrel of a syringe The diameter of the hollow barrel is about mm and the diameter of the syringe nozzle is about 1.2 mm The distance between the tip of the HV ... evaluate the inactivation role of heat, the gas temperature of the plasma plume is determined by comparing experimentally measured emission spectrum of the second position system of nitrogen with...
... by the blue line in Fig 4g, the critical thickness for the onset of this roughening transition is determined as 4.1 ML The surface structure formed in this roughening transition is revealed by ... not increase much, because once formed practically all of the subsequently deposited Ge is transferred and incorporated into the 3D islands This is substantiated by statistical analysis of the increase ... line in Fig 4g) The latter is caused by the surface roughening of the 2D Ge wetting layer surface associated with the formation of a high density of dimer vacancy lines and subsequently missing...
... statistical result from TEM Principle ofInSitu Mineralization of Magnetite in Chitosan Hydrogel Fig TG curves of pure magnetite (a) and the mineralized magnetite nanoparticles (b) existence of ... were inevitably coated by a thin layer of chitosan Moreover, the TEM morphology of mineralized magnetite nanoparticles proved the existence of chitosan layer In order to obtain the amount of chitosan ... on mineralization of magnetite in organic hydrogel have great scientific and practical significance Inspired by magnetic bacteria, we propose insitu mineralization of magnetite nanoparticles in...
... bFGF within MSNs insitu The in vitro releasing kinetics of bFGF from MSNs has been investigated through colorimetric enzyme linked immunosorbent assays (ELISAs) It is expected that this new delivery ... Encapsulation of bFGF in MSNs A weak acidic water -in- oil method followed by ammonium hydroxide treatment was developed to encapsulate bFGF insitu Figure illustrates the insitu loading of bFGF in MSNs ... loading efficiency (%); M0 , the total amount (wt) of bFGF loaded in nanoparticles, which is converted from C0 determined by ELISA kit; M0, the original amount (wt) of bFGF, that is, 10 lg In Eq...
... adsorption was done by exposing the surfaces in a 10-2 M solution of HAuCl4Á3H2O for h followed by rinsing with water and finally drying the surfaces under a flow of argon Insitu reduction to generate ... earlier discussion of AFM results points to the possible core–shell structure formation during insitu synthesis of Au–Pt NPs on the surface from the observed increase in nanoparticle size going from ... analysis software Surface coverage of different nanoparticles was estimated by analyzing the mean grain area and number of grains present in a typical lm lm (total area = lm2) AFM image of the...
... represents a starting point of a three-dimensional MWNT network in the matrix, and can be determined from the starting / at which the viscosity does not show Newtonian but shear-thinning behavior ... and then quenching them in icy water, did mostly a-phases This difference might be due to the shear stress during electrospinning Acknowledgement Financial support from Asian Of ce of Aerospace ... 4:39–46 41 Characterization Scheme Insitu polymerizations of the F-MWNT/nylon-6 and the P-MWNT/nylon-6 bottom flask The weight % of the input MWNT is denoted as / in this article The mixture was sonicated...
... example of Atticus; incoming of wealth after Hannibalic war; suddenness of this; rise of a capitalist class; the contractors; the public contracting companies; in the age and writings of Cicero; their ... political influence; and power in the provinces; the bankers and money-lenders; origin of the Roman banker; nature of his business; risks of the money-lender; general indebtedness of society; ... OF THE VILLA OF DIOMEDES AT POMPEII PLAN OF A TRICLINIUM MAP ROME IN THE LAST YEARS OF THE REPUBLIC At end of Volume Translations of passages in foreign languages in this book will be found in...
... indebted to Professor Bussey of the University of Minnesota, Professor Roever of Washington University, Professor Kempner of the University of Illinois, and Professor Young of the University of Chicago ... CHAPTER VIII Determinants; Systems of Linear Equations Solution of Linear Equations by Determinants, 115 Solution of Linear Equations by Determinants, 116 Signs of the Terms of a Determinant, 117 Even ... circle having as a diameter the line BQ joining the points B = (0, 1) and Q = (a, b) in Fig Then the abscissas ON and OM of the points B N of intersection of this circle with the O x-axis are the...
... potential data (Bethenod et al., 1988) This scatter can be induced by: 1) individual variability 2) changes in A between morning and evening at the same level of incident and PPFD The maximum values ... summarized by these asymptotes (Fig 5) H is the point where the maximum of demand function crosses the C;regulation line We can observe that, if WUE of maize is higher than the WUE of lilac or ... and the slope of this line represents C Beyond these values, i - C a A does not increase for both C plants, although g can be large for lilac Conse quently, phases exist in this A - g relac...
... during infectious processes making it necessary to examine the presence and localization of both proteins In view of these facts, it is pivotal to gain insight in the cellular expression of caveolae ... It is tempting to speculate that certain caveolae may exist that contain predominantly the β-isoform Such caveolae might be involved in other signaling cascades If both isoforms are present in ... (EGF), leading to neoformation of caveolae [33] Since EGF is considered as a key factor for repair of the bronchial epithelium [34], caveolae containing the α-isoform might be involved in this process...
... +Ap+Ch 10-5 M) in isolated pulmonary artery Characterisation of the dilator effects of acetylcholine (ACh; Figure Characterisation of the dilator effects of acetylcholine (ACh; 10-5 M) in isolated ... and airways in vitro In lung slices, the dilator effects of acetylcholine on U46619 constriction tissue was blocked by L-NAME or by the combination of apamin plus charybdotoxin L-NAME is a highly ... paralleled by responses of isolated pulmonary artery or bronchi in vitro In isolated pulmonary artery preparations, the vasodilator effects of acetylcholine were unaffected by indomethacin, suggesting...
... and the use of fresh tissue, as staining in frozen tissue results in low signal to noise ratio [11-13] In this paper, we report technical improvements in staining frozen tissues using commercially ... verifying that nonspecific binding of the Qdot 655 multimer is low Staining pattern and staining intensity of Gag CM9 Qdot 655 multimer positive cells Confocal microscopy revealed a punctate staining ... Gag CM9 positive cells dispersed in the lamina propria (Fig 4F) Table Percentage of Gag CM9 positive cells quantified by imaging analysis ofinsitu stained lymphoid tissue sections ID No Specimen...
... G.3 Far-infrared Region A representative spectra of the far-infrared region, 400-700 cm-1, are shown in Figure G.1 Figure G.1 In- situ raw far-infrared spectra of the hydroformylation of 3,3-dimethyl-1-but-ene ... the highpressure cell were recorded Hydroformylation reaction was initiated by introducing MPa of H2 gas Then, spectra were recorded at 10 minute intervals in the range 400-2500 cm-1 The semi-batch ... Preliminary FIR Analysis Appendix G Preliminary FIR Analysis The current appendix presents the first attempt of analyzing the far-infrared region The collected spectra were obtain from monitoring...