interaction of electron beam with electromagnetic field

... behavior of trehalase depending on the nature of the activation stress (Fig. 5B). This shift might argue against the participation of a unique set of interactions involving association of Ntp1p ... identification of strains C3 and C69, with one copy of Ntp1p–Ha6H expressed from the genomic ntp1 + promoter, was verified by Southern blot analysis and immunoblot of whole-cell extracts with anti- Ha ... the regulation of Ntp1p activity under stress relies on the existence of some kind of interaction between Ntp1p and Tps1p, or other elements, that may be lost under the conditions of the in vitro...

Ngày tải lên: 08/03/2014, 23:20

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Ngày tải lên: 18/06/2014, 22:20

Ngày tải lên: 20/06/2014, 04:20

... is a consequence of exertion of the mechanical sub-system on the electromagnetic sub- system. IV. M ATHEMATICAL M ODEL OF T HE VCS Description of one type of two-mass electromagnetic vibratory ... half- wave with f d =50Hz. It has been simply realized with comparator tolerance band i.e. hysteresis (“bang-bang”) controller. Reference current was compared with actual current with the tolerance ... case of thyristor converter, with phase control, LCE displacement has “smooth” sine characteristic, although EVA current is pulsating. Also, change of vibratory width is due to change of phase...

Ngày tải lên: 03/01/2014, 19:16

... into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp Vasthi ... spite of the rel- atively low agmatinase activity of the Asn149Asp vari- ant, it is clear that the interactions of arginase II with l-arginine and agmatine are greatly altered by replace- ment of ... blue staining of purified enzymes. Metal-free species of purified enzymes were obtained by incubation for 1 h at 25 °C with 25 mm EDTA and 3 m V. Lo ´ pez et al. Interaction of arginase II with substrate...

Ngày tải lên: 20/02/2014, 02:21

... intensity of the band of unhydrolyzed actin was plotted against the time of incubation. Characterization of actin preparation quality Fluorescence parameters were used for estimation of nativity of ... aggregation of partially folded actin. To obtain more detailed information on the interaction of HSP25 with partially unfolded actin, we analyzed the effect of different quantities of HSP25 on ... parameter A of actin was close to 2.4. Increase of the time of heating at 43 °C leading to decrease of parameter A up to 2.2–2.3 was accompanied by further decrease of the initial rate of polymerization....

Ngày tải lên: 20/02/2014, 23:20

... probable regions of interaction, in agreement with the contacts with GrpE and the results obtained from experiments with mutants. The contact r egions predicted w ith our method and t he implicit model of interaction ... of the protein structure is the detection of regions of interaction by the presence of specific family signatures in the m ultiple s equence alignment a ble t o discriminate different t ypes of ... retained, independent of their classification. Furthermore, a 40% sequence identity cut-off for protein homology is used instead of the present 30% and the definition of the interaction surface is...

Ngày tải lên: 21/02/2014, 15:20

... spectra of a recombinant decorin purified in the absence of chaotropic agents, with the exception of the wavelength of the minimum (215–216 instead of 218 nm) and very different to the spectrum of ... 2002 Interaction of decorin with collagen peptides (Eur. J. Biochem. 269) 1431 with respect to the unmodified ones is not related to the percentage of Lys/Hyl side chains that did not react with ... these data demonstrate the essential role of the positive charge of collagen Lys/Hyl residues for interaction with decorin. DISCUSSION The binding of decorin with fibrillar collagens has been extensively...

Ngày tải lên: 21/02/2014, 15:20

... basal levels of resonance units (GlnK free surface) within seconds. To test the effects of various molecules on the interaction of TnrA with GlnK, 40 nm GlnK (trimer) Fig. 1. Coimmunoprecipitation of TnrA, ... equilibrated with 10 column volumes (10 · 0.2 mL) of buffer B, with subsequent washing four times with five volumes of the same buffer. Proteins were eluted with buffer E (buffer B supplemented with 250 ... (SPR) of the GlnK–TnrA interaction As a next step, the interaction of TnrA with GlnK was investigated in vitro by BIAcore SPR detection. For this analysis, a Strep-tag II-tagged variant of GlnK (GlnK-ST)...

Ngày tải lên: 06/03/2014, 00:21

... for the interaction of frog annexin A1 and annexin A2 with dicalcin. Consistently, we observed that the 35 kDa forms of annexin A1 and annexin A2 found in the fraction of the binding proteins of dicalcin (Fig. ... presence of dicalcin and all of the annexins (E) was compared with the calcu- lated sum of each of the initial rates obtained in (B)–(D) (thick dot- ted lines). T. Uebi et al. Role of dicalcin ... 4869 Comprehensive interaction of dicalcin with annexins in frog olfactory and respiratory cilia Tatsuya Uebi 1 , Naofumi Miwa 1,2, * and Satoru Kawamura 1,2 1 Department of Biology, Graduate School of Science,...

Ngày tải lên: 07/03/2014, 05:20

... Recognit. 16, 91–101. Ó FEBS 2004 Interaction of human TCR with superantigen SSA (Eur. J. Biochem. 271) 4083 Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen ... Vb5.2 and V b1withK d of 9–11 l M with a fast k ass and a moderately fast k diss . In spite of the reported stimu- lation of V b2.1 bearing T-cells by SSA, we o bserved no measurable interaction. Keywords: ... Consequently, the molecular studies of the interaction of SAgs with their s pecifics ligands will not only advance understanding of the physiological mechanisms of these molecules, but may lead to...

Ngày tải lên: 07/03/2014, 16:20

... report on the interaction of an N-terminal (E2(7–26)) and an internal (E2(279–298)) synthetic peptide sequence of the E2 structural pro- tein of HGV ⁄ GBV-C with phospholipid membranes of different ... short hydrophobic segments of viral envelope glyco- proteins with a very low content of hydrophilic amino acids, the presence of acidic residues in the fusion pep- tides of some low-pH-activated ... strongly interacted with PtdCho ⁄ PtdSer (65 ⁄ 35) vesicles, as the blue shift of Trp was 11 nm. To study the contribution of electrostatic inter- actions to the binding of both peptides with negatively charged...

Ngày tải lên: 07/03/2014, 17:20

... transmis- sion electron microscopy (TEM) and atomic force microscopy [15]. In this study, we measured the interaction of stefin B with various combinations of phospholipid monolayers and bilayers. Interaction ... channel hypothesis of AD and neurodegeneration in general, is not incompatible with other key elements of toxicity, as, for example, the deregulation of Ca 2+ homeostasis and generation of reactive ... membranes, initiating a cascade of detrimental events for the cell. Interaction of granular aggregates and globular oligo- mers of an amyloidogenic protein, human stefin B, with model lipid mem- branes...

Ngày tải lên: 07/03/2014, 17:20

... predicted protein is composed of 306 amino acids, with a calculated molecular mass of 34.3 kDa and a pI of 5.72. Similar searches with the deduced amino-acid sequence of f ull-length Calliphora AFP, ... according to the following scheme: 3 · 10 min with 4 · NaCl/Cit; 2 · 10minwith2· NaCl/Cit; 1 0 min with 0.1 · NaCl/Cit; 10 min with 0.05 · NaCl/Cit; 5 min with NaCl/Tris. After incubation for 30 m ... encoded a protein with 93% identity in the deduced amino-acid sequence with the A FP of S. peregrina (GenBank accession number BAA99282). Because of the high sequence identity with the Sarcophaga...

Ngày tải lên: 08/03/2014, 22:20