integrins 5 and 1 expression and live bcg internalization profiles of human bladder cancer cell lines
Mycobacterium bovis, BCG modulation of gene expression in bladder cancer
... recruited to the bladder and iliac lymph nodes 81 Table 4 .1 Integrins 5 and 1 expression and live BCG internalization profiles of human bladder cancer cell lines 87 Table 4.2 Effects of blocking ... Integrin expression and BCG internalization profiles of human bladder cancer cell lines ……………………………………………………………………………… 87 4 .1. 1 Expression of Integrin 5 on human bladder cancer cells correlates ... Introduction……………………………………………………………………… 1. 1 The prevalence and challenges of bladder cancer ……………………………… 1. 1 .1 The stages of bladder cancer …………………………………………………… 1. 1.2 Superficial bladder cancer ……………………………………………………… 1. 1.3 Advanced...
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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
... Program of the UNR ECS is member of the National Research Council (CONICET, Argentina) and JEG is Fellow of the same institution 10 11 12 13 14 15 16 17 REFERENCES Hayes, J.D & Pulford, D.J (19 95) ... glutathione Ethacrinic acid Hematine CDNB I50 (lM) 12 .5 0.3 < 0.0 01 < 0.0 01 0 .1 6.4 5. 3 I50 (lM) 4.8 8.3 < 0.0 01 < 0.0 01 238 4 .5 < 0.0 01 Fig pH optimum of SmGSTO The SmGSTO pH profile was carried ... Biochem 269) 55 17 Fig Expression and purification of His-tagged SmGSTO and native SmGSTO (A) SDS/PAGE analysis of His-tagged SmGST purified on nickel-agarose Lane 1, 50 lg soluble extract of BL 21 (ED3)...
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Báo cáo khoa học: Vaccination with prion peptide-displaying papillomavirus-like particles induces autoantibodies to normal prion protein that interfere with pathologic prion protein production in infected cells pptx
... in mice J Immunol 11 1, 14 07 14 10 10 Klein MA & Aguzzi A (2000) The neuroimmune interface in prion diseases News Physiol Sci 15 , 250 – 255 11 Enari M, Flechsig E & Weissmann C (20 01) Scrapie prion ... concentrations of Abs to epitopes encompassing amino acids 95 1 05 of the PrP were used Injection of mAbs that recognize the central region of the PrPC (residues 13 3– 15 7 ) and bind efficiently to cell surface ... reduce the PrPC and PrPSc glycoforms to one single band [26,48] Both Ab A7 and mAb 4H 11 recognize each of the PrP glycoforms The samples were analyzed on 12 .5% SDS polyacrylamide gel, and this was...
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Báo cáo khoa học: Purification and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella germanica pptx
... 3.2 0.63 0.34 1. 06 ND ND ND ND ND ND 14 4 a ± ± ± ± ± ± DmGSTD1 49 0. 15 0 .1 0. 01 0.07 0.03 NlGST1 -1 AgGSTD6 58 .1 0 .13 0.27 14 1 0.09 0 . 51 0. 05 ND 1 95 0.64 0.98 < 0. 15 0 .10 2 864b 7. 71 )1 Activity in ... DmGSTD1 NM_079602 NlGST1 -1 LcGST1 AgGSTD3 BmGST1 MsGSTolf1 AgGSTD7 AgGSTS1 Bla g DmGSTS1 AF44 850 0 L2 312 6 AF273039 AB1766 91 AF133268 AF0 711 61 AF 513 639 U92 412 NM _16 6 217 MsGST2 AgGSTE2 AgGSTE1 AgGSTO1 ... reported to date 85 80 Purified 75 GST Re lative Ab sorba nce 70 Amino acid sequencing 65 60 55 50 45 40 35 30 25 20 15 10 0 10 15 20 25 Time (min) 30 35 40 45 50 Fig Purification of cockroach GST...
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Báo cáo khoa học: Transfection with 4-hydroxynonenal-metabolizing glutathione S-transferase isozymes leads to phenotypic transformation and immortalization of adherent cells pdf
... preparation of recombinant GST isozymes mGSTA4-4 [14 ], Drosophila melanogaster DmGSTD1 -1 [17 ], hGSTA1 -1 [18 ] and hGSTP1 -1 [19 ] have been described previously Preparation of hGSTA4-4 eukaryotic expression ... activities: 65 UÆmg )1 and 32 UÆmg )1, respectively) On the other hand, human enzymes hGSTA1 -1 and hGSTP1 -1 have minimal activity towards 4-HNE [27] These results show that mGSTA4-4 and DmGSTD1 -1 (Fig ... hGSTA44(Y 212 F), mGSTA4-4, DmGSTD1 -1, hGSTA1 -1, and hGSTP1 -1] indicates that a causal involvement of 4-HNE in the mechanism of hGSTA4-4-mediated transformation of HLE B-3 and CCL- 75 cells provides...
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Báo cáo khoa học: Purification and partial characterization of seven glutathione S -transferase isoforms from the clam Ruditapes decussatus pptx
... substrateÆmin)1mg protein )1) CDNB ETHA 7.20 26 9.02 24 12 .06 24 14 .09 25 14 .83 25 16 .40 23 12 .06 /16 .40 24/23 38 ± 30 59 ± 12 467 ± 41 30 ± 290 ± 17 15 ± 270 ± 35 4.4 ± 2.4 86 ± ND 3 61 ± 21 50 ± 10 91 ± 10 .1 ... C1E2 C2E1 C2E2 0– 450 0– 350 0–200 0– 350 3 3 450 –640 350 –400 200 55 0 350 – 450 12 11 12 12 650 – 750 400– 450 55 0– 750 450 – 750 5 subunits of the purified isoforms N-terminal amino acid analysis was carried ... (lmolÆmin )1 mg )1) Vmax (GSH) (lmolÆmin )1 mg )1) Km (CDNB) (mM) Km (GSH) (mM) kcat (s )1) kcat/Km (CDNB) (mM )1 s )1) kcat/Km (GSH) (mM )1 s )1) 204 .1 ± 18 1.6 ± 0 .17 ± 4.82 ± 214 .3 ± 12 44.97 44. 417 GST 5- 5...
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Báo cáo Y học: Selection of effective antisense oligodeoxynucleotides with a green fluorescent protein-based assay Discovery of selective and potent inhibitors of glutathione S-transferase Mu expression doc
... – 36 55 11 9 13 8 15 2 17 1 1 75 19 4 – 326–3 45 397–426 480–499 52 4 54 3 – – 786–8 05 – – 978–997 – – – 11 9 13 8 15 2 17 1 1 75 19 4 2 85 304 – – – 52 4 54 3 55 4 57 3 655 –674 – 870–889 9 21 940 – 0 13 10 12 a ... EGFP-M1 AS-ODN % of EGFP control % of EGFP control % of EGFP-M1 control % of EGFP control % of EGFP-M2 control – AS -5 AS-6 AS -10 10 0 93 99 95 340 207 11 0 244 10 0 51 61 190 11 8 14 6 1 25 10 0 ± 23 ... cotransfection of PS-ODNs per se does not influence EGFP expression AS-6 and AS-8, directed against GSTM1, inhibited EGFP–M1 expression by 95 ± 1% and 81 ± 6%, respectively (Fig 3A) The expression of EGFP and...
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Báo cáo y học: "4-Hydroxynonenal induces apoptosis in human osteoarthritic chondrocytes: the protective role of glutathione-S-transferase" doc
... Rheum 19 98, 41: 1266 -12 74 12 Esterbauer H, Schaur RJ, Zollner H: Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes Free Radic Biol Med 19 91, 11 : 81- 128 13 Poli ... product of medicinal interest Med Res Rev 2008, 28 :56 9-6 31 14 Barrera G, Pizzimenti S, Laurora S, Briatore F, Toaldo C, Dianzani MU: 4-hydroxynonenal and cell cycle Biofactors 20 05, 24: 15 1 - 15 7 15 Cerbone ... Cartilage 2004, 12 :13 1 -14 1 Hashimoto S, Ochs RL, Komiya S, Lotz M: Linkage of chondrocyte apoptosis and cartilage degradation in human osteoarthritis Arthritis Rheum 19 98, 41: 1632 -16 38 Murrell GA,...
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Báo cáo y học: " Genetic polymorphisms in glutathione S-transferase (GST) superfamily and risk of arsenic-induced urothelial carcinoma in residents of southwestern Taiwan" ppt
... HR ( 95% CI) Count(%) Follow-up person-years UC Crude incidence (per 1 05) < 50 390 ( 51 .1) 7 2 51 11 15 1 .7 ref 50 -59 270( 35. 3) 450 8 22 488.0 3.38 (1. 64-6.98)** 60+ 10 4 (13 .6) 15 5 7 51 3.8 3.68 (1. 48-9 .18 )** ... 0.79 0.02 GSTP1 -1 05 AA 262 ref 11 2 12 ref AG 93 0. 41( 0. 05- 3. 35) 0. 41 47 1. 41( 0 .55 -3.62) 0.47 GG 36 0.00 0.99 22 1. 76(0 . 51 -5. 73) 0. 35 AG+GG GSTO1 -14 0 12 9 0.33(0.04-2.66) 0.30 69 11 1 .52 (0.66-3.48) ... AA 4 91 (64.8) 25 ref AG 19 2 ( 25. 3) 10 1. 07(0 . 51 -2.23) 0.86 GG AG+GG 75 (9.9) 267 ( 35. 2) 15 1. 71( 0.64-4 .56 ) 1. 21( 0.64-2. 31) 0.29 0 .56 CC 52 1 (70.0) 27 ref CA 19 9 (26.7) 0. 81( 0.38 -1. 73) 0 .59 AA...
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Báo cáo y học: " Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients" potx
... 83 (5) 99 (12 ) 96 (10 ) 10 1 (12 ) 52 (6) 4 :1 28 (11 ) 10 2 ( 15 ) 82 (5) 10 3 (12 ) 84 (13 ) 10 2 (6) 63 (8) 8:7 47 (12 ) 62 (18 ) 64 (10 ) 31 (19 ) 65 (12 ) 76 (17 ) 0.06 0 .10 3 0.000 0.0 01 0.0 01 0.0 01 0.0 01 0.003 ... p-value 62 (13 ) 4 :5 99 (20) 88 (6) 94 (29) 92 (18 ) 98 (18 ) 60 (3) 4 :1 41 (14 ) 84 (14 ) 81 (7) 84 ( 61) 81 (11 ) 88 (1) 61 (9) 13 :4 35 ( 15 ) * 50 (28) 55 (17 ) 37 (20) 54 (22) 61 (22) 0.0 917 0. 350 0.000 ... (11 ) 63 (8) 17 :5 46 (19 ) 90 (10 ) 83 (11 ) 80 (37) 78 (14 ) 83 (12 ) 62 (9) 27:7 38 (13 )* 55 (23) 56 ( 15 ) 34 ( 21) 64 (27) 72 (24) 0 .54 3 0 .10 3 0.000 0.000 0.000 0.000 0.004 0.0 35 Mean (SD) *In post-hoc...
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Báo cáo y học: "Anticitrullinated protein antibody (ACPA) in rheumatoid arthritis: influence of an interaction between HLA-DRB1 shared epitope and a deletion polymorphism in glutathione s-transferase in a cross-sectional study" ppt
... 610 ) 64 (11 ) 53 ( 15 ) 655 (93%) 17 3 (28%) 53 6 (76%) 420 (69%) HLA-DRB1 SE-positive 53 1 (76%) 434 ( 71% ) One copy 356 ( 51 %) 303 (50 %) Two copies 1 75 ( 25% ) 13 1 ( 21% ) GSTM1-null 372 (53 %) 3 15 (52 %) SE-GSTM1 ... (0.42 to 1. 22) 0. 75 (0.42 to 1. 36) 0.223 0.349 Null/Ever 81 2. 01 (1. 13 to 3 .55 ) 0. 017 70 0.83 (0.49 to 1. 42) 0 .50 2 AP = 0 .12 ( -1. 41 to 1. 65) AP = 0.44 (-0.28 to 1. 15 ) Padd = 0.8 81 Padd = 0.2 31 Pmult ... 3.23 (2 .17 to 4. 81) < 0.0 01 < 0.0 01 75 68 2 .56 (1. 77 to 3.70) 1. 77 (1. 21 to 2.60) < 0.0 01 0.003 SE-positive (two alleles) 93 10 . 65 (5. 61 to 20.20) < 0.0 01 92 10 .27 (5. 05 to 20.89) < 0.0 01 a ACPA,...
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Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt
... and oxidation W J J Firdaus et al C 25Q 25Q +NAC Counts 40 80 12 0 16 0 200 A 10 1 10 2 FL2-H 10 3 10 4 72Q 72Q+NAC Counts 40 80 12 0 16 0 200 10 0 10 1 10 2 FL2-H 10 3 10 4 B 10 3Q 10 3Q+NAC Counts 40 80 12 0 ... 30 91 Huntingtin inclusions and oxidation 44 45 46 47 48 49 50 51 52 53 54 55 56 57 W J J Firdaus et al dehydrogenase activity and by maintaining optimal cellular detoxifying machinery Exp Cell ... reduces levels of mutant huntingtin and the risk of neuronal death Nature 4 31, 8 05 810 18 Kopito RR (2000) Aggresomes, inclusion bodies and protein aggregation Trends Cell Biol 10 , 52 4 53 0 19 Ravikumar...
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Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... parameters (A) c = 11 6.22 c = 11 6.2 Wavelength (A) 0. 710 0 1. 0000 Resolution range (A) 50 1. 05 (1. 0 91. 05) 50 1. 71 (1. 7 71. 71) Measured reections 950 , 955 2 05, 263 Unique reections 89,930 21, 327 Completeness ... the interior of the dimer interface, and Leu10 and Tyr12 form a hydrophobic core with Ile70, Met 116 , Val1 41, Pro 15 4 , Ile 15 6 and Pro1 61 of the other monomer Similarly, Gly175Ala1 81 forms an anti-parallel ... structure of Structure of avoredoxin 10 11 12 13 14 15 16 17 18 19 FMN-binding protein differ? Acta Crystallogr D Biol Crystallogr 56 , 3683 71 Fontecave M, Coves J & Pierre JL (19 94) Ferric reductases...
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Analysis of Healthcare Interventions that Change Patient Trajectories doc
... Trajectories 3.20 3. 21 3.22 3.23 3.24 4 .1 5. 1 5. 2 5. 3 5. 4 5. 5 5. 6 5. 7 5. 8 5. 9 5. 10 5. 11 5. 12 5. 13 5. 14 5. 15 5 .16 5. 17 5. 18 5. 19 5. 20 6 .1 6.2 6.3 6.4 6 .5 6.6 6.7 Projections of NHE Expenditures ... Supplies and Equipment Prescription Drugs 19 96 19 97 19 98 19 99 2000 1. 278 1. 273 1. 250 1. 279 1. 266 1. 290 1. 208 1. 178 1. 262 1. 146 1. 1 21 1 .1 95 1 1 .12 0 0.7 65 1. 2 65 0. 952 1. 0 51 1. 033 0. 858 1. 104 1. 004 ... 0.960 1. 002 0.640 0.942 0.6 61 1 .1 15 1. 0 41 0.7 81 0.963 0.7 45 1. 169 1 1 0 .59 6 0. 655 0.607 0.8 75 1. 15 2 1. 064 1. 052 1. 076 1. 174 0.946 1. 108 0.942 1 0.943 1. 1 31 0.997 1. 262 0.978 1. 029 0.978 1. 029 1 1.332...
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Báo cáo khoa học: Epitope analysis of the rat dipeptidyl peptidase IV monoclonal antibody 6A3 that blocks pericellular fibronectin-mediated cancer cell adhesion pot
... 654 8– 655 9 ª 2009 The Authors Journal compilation ª 2009 FEBS 655 1 Epitope of monoclonal DPP IV antibody T.-T Hung et al A 29 13 1 50 3 A B 767 D Ver 17 3749 50 3 G C 767 D Ver B kDa A 10 0 75 50 10 0 ... another two metastatic cancer cell lines, human breast cancer cells (MDA-MB2 31) and mouse melanoma cells (B16F10), for rDPP IV adhesion assays Similar to MTF7, MDA-MB-2 31 and B16F10 exhibited high ... 50 10 0 75 50 10 0 75 50 D A C B * + D G + DexFL * C kDa IB:6A3 * * * * * * Hm IB:6A3 50 * IB:MBP B -16 6 E B -16 6 kDa WT 75 IP:6A3 IB:MBP * * D 29 27 B (3 15 16 BB- B- ) 58 ~3 * I 31 Mm kDa 75 T3 T...
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Báo cáo sinh học: "Analysis of the human cytomegalovirus genomic region from UL146 through UL147A reveals sequence hypervariability, genotypic stability, and overlapping transcripts" ppt
... BI -5 C 954 E5 45 E763 E460 CH 25 CH23 C322 CH18 10 0 76 10 0 10 0 10 NW23 -1 PT2 PT1 10 0 PT 11 11 C239 C124 E428 CH20 99 C 952 C2 21 E 759 C 956 CH 15 12 13 14 CH5 CH1 CH2 CH14 CH22 10 0 71 100 93 10 0 10 0 PT12 ... urine urine 255 years 15 W1 W1 D+R- C3 L D+R+ urine 11 1 12 L1 C1 917 C198 Z Z D+R- urine urine 216 2.6 years L1 L1 X162 C 118 C 954 C C C D+R+ urine urine urine 55 55 55 16 C1 C1 C1 C69 C172 Y Y D+R- ... 74 32 P1 P1 P1 C 257 1 C 952 G G D+R+ urine urine 54 54 24 G1 G1 C194 C8 75 F F D+R+ urine urine 10 1 10 1 F1 F1 C184 C2 21 T T D+R- urine urine 54 54 22 T1 T1 C108 C246 C124 C3 45 R1 R1 R1 R1 D+R+ blood...
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Báo cáo hóa học: " Hepatitis C virus NS5A protein binds the SH3 domain of the Fyn tyrosine kinase with high affinity: mutagenic analysis of residues within the SH3 domain that ?" doc
... SD) n = 4.93 × 10 3 (± 0.66 × 10 3) 9. 05 × 10 3 (± 1. 62 × 10 3) 3.08 × 10 -3 (± 0 .16 × 10 -3) 4.94 × 10 -3 (± 0 .11 × 10 -3) 6.29 × 10 -7 (0 .59 × 10 -7) 5. 56 × 10 -7 (0 .59 × 10 -7) dicted to make major contributions ... 2004, 279:4 857 6-4 858 7 Page of (page number not for citation purposes) Virology Journal 2008, 5: 24 10 11 12 13 14 15 16 17 18 19 20 Tellinghuisen TL, Marcotrigiano J, Rice CM: Structure of the zincbinding ... D100, W 119 , N136 and Y137, were preTable 1: Kinetic parameters for the NS5A:Fyn SH3 domain interaction Protein ka (M-1s -1) kd (s -1) KD (M -1) NS5A(Δ32) (± SD) n = NS5A(Δ 250 ) (± SD) n = 4.93 × 10 3...
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báo cáo hóa học:" High rate of loss to clinical follow up among African HIV-infected patients attending a London clinic: a retrospective analysis of a clinical cohort" ppt
... stage n = 11 25 n = 303 n = 322 Asymptomatic Symptomatic 734 ( 65. 2%) 232 (20.6%) 1 85 ( 61. 1%) 80 (26.4%) 224 (69.6%) 61 (18 .9%) AIDS < 0.0 01 37 (11 .5% ) 0.0 01 0.0 91 15 9 (14 .1% ) 38 (12 .5% ) Received ... (0.86-2.24) 0 .17 4 1. 64 (0.98-2. 75) 0.060 < 200 12 6 (13 .3%) 3.68 (2. 25- 6.03) < 0.0 01 1.99 (1. 05- 3.74) 0.034 n = 939 8 25 (87.7%) 1. 00 (REF) 11 4 (12 .1% ) 6.46 (4.09 -10 . 21) < 0.0 01 5. 03 (2. 95- 8 .57 ) Viral ... (0.68-0.83) < 0.0 01 2003-20 05 255 1. 00 (REF) 2000-2002 19 1 2.77 (1. 88-4.09) < 0.0 01 2. 91 (1. 77-4.78) < 0.0 01 1997 -19 99 86 4.70 (2.78-7.94) < 0.0 01 5. 26 (2. 71- 10 .19 ) < 0.0 01 Year of KCH HIV clinic...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25