... profiles of this cDNA subset were examined here in further detail and compared to one another to gain an understanding ofthe timing in transcript accumulation changes occurring during the early ... speculate that the biosynthesis of anthocyanins, proanthocyanidins and flavonols is relegated to second place compared to that ofthe isoflavones/ isoflavanones-phytoalexins during the soybean resistance ... participates inthe cascade of reactions elicited during defense, although at a later time relative tothe isoflavone/phytoalexin branch ofthe pathway F3'H hydroxylates naringenin to eriodictyol a...
... that measurement of acid phosphatase activity was the best estimation ofthe mRNA abundance in our systems, and from here on we use the ratio of acid phosphatase activities as an indicator of ... four ofthe strains being wild-type for SPT6 and four of them having a spt6–140 allele The average ofthe acid phosphatase activities was used to calculate the ratios between those cells expressing ... detected effect ofthe SAGA mutants on the GLAM ratio would not be due to an indirect in uence of altered chromatin structure but toa sustained role of SAGA after the initial steps of transcription...
... Jon A Arnot and Frank A P C Gobas (ii) the parameterization ofthe model to reflect Canadian conditions and (iii) the calibration ofthe model toa large BCF and BAF database The resulting QSAR ... of application ofthe BAF-QSAR include the categorization of bioaccumulative substances, the derivation of water quality criteria and the estimation of total maximum daily loadings for aquatic ... growing and/or (iv) metabolize the substance at a significant rate The BAF-QSAR exhibits a ™parabolic∫ shape At low KOW, the BAF increases with increasing KOW ina linear fashion, as partitioning of...
... Station, Texas New York Philadelphia Washington, D.C Boston Washington, D.C Minneapolis East Lansing Philadelphia East Lansing Boston Philadelphia Atlanta North Miami Charlotte, North Carolina ... trees around the Tidal Basin, the stately elms gracing the Reflecting Pool, and massive oaks of Lafayette Park, there are 4,839 acres of general tree cover inthe city’s 7,999 acres of parkland Their ... PARK VALUE IN ACTION Cutting Air Pollution Costs in Washington, D.C The trees of Washington, D.C., are the city’s lungs, inhaling and exhaling the air flowing around them Beyond the famous Japanese...
... in infrastructure Data analysis included both quantitative (factor analysis and structural equation modeling) and qualitative (thematic content analysis) methods as explained in Chapter A ... Policy makers will therefore need to pay careful attention to all ofthe factors that determine the changes and their impacts on the aggregate growth rate 2.3.3.2 Theory of coordination failure The ... consultants and contractors involved in investing and delivering infrastructure, as well as in utilizing infrastructure services for trading activities There are four major factors that the thesis...
... membranes ap- early and conductivity relatively stable, at least values remained up to 72 h, after which deterioration apparent (again, more prominent at 30°C) Of particular interest was the differential ... on other assay responses (Webber, unpublished data) Work must continue to evaluate the effect of hydration on viability responses for all species and, in particular, investigate the effect of ... In addition to an ageing effect, which was accelerated by 30°C and 100°C RH, there was also a shock effect which was prevalent inthe early stages of hydration and most severe for 30°C It was...
... were treated with increasing concentrations of H2O2 for 10 Akt activation and FOXO 3a phosphorylation were analyzed by Western blot using anti-activated Akt antibodies and anti-phospho-FOXO 3a antibodies, ... that H2O2 treatment down-regulated expression of p27 at mRNA levels Notice that the p27 is a negative image ofthe gel The experiments were done as inA Total RNA was isolated from the cells and ... anti-p66shcA antibodies and protein A beads, washed with lysis buffer, and analyzed by Western blot using anti-ERK antibodies Rabbit IgG was used as a control inthe assays One twentieth ofthe cell...
... p46ShcA with PKC δ p52ShcA or p46ShcA and associated proteins were precipitated with anti-ShcA antibodies and PKC δ was detected with an anti-PKC δ antibody Bottom panel: quantitation data from AThe ... p52 and p46 Shc due tothe use of two start codons Bottom panel: quantitation data from B The value of co-immunoprecipitated p52Shc or p46Shc inthe absence of H2O2was set at -95- C IP: anti-HA ... challenged with H2O2 ERK activation was analyzed with Western blot (B) Quantitation data from AThe basal level of activated ERK2 was set at Rott: Rottlerin; Stau: staurosporin -105- ...
... presence inthe tongue may indicate an origin ina minor salivary gland Adenocarcinomas ofthe tongue are rare and represent the minority (20 to 25%) ofthe salivary gland tumors affecting the tongue ... mechanisms of cancer and cancerrelated signaling pathways, such as mammalian target of rapamycin (mTOR) signaling, p53 signaling, Mycmediated apoptosis signaling, vascular endothelial growth factor ... Auris Nasus Larynx 2004, 31:85-88 Tanaka T, Watanabe T, Kazama Y, Tanaka J, Kanazawa T, Kazama S, Nagawa H: Chromosome 18q deletion and Smad4 protein inactivation correlate with liver metastasis:...
... revised the manuscript critically AK performed pre-processing and statistical analysis ofthe microarray data CB conducted adaptations ofthe GeneTrail software forthe use of Arabidopsis thaliana ... the National Institute on Aging [21] was utilized The statistical analysis performed with this online tool was based on the single-factor ANalysis Of VAriance (ANOVA) The statistical significance ... www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE24348 Statistical analysis of microarray expression data and calculation of fold changes For further data analysis, the data extracted from the Affymetrix Microarray Suite...
... scanner (Amersham Biosciences) Data analysis for DNA-array Data analysis was performed using ImageQuant software (Molecular Dynamics, Sunnyvale, CA) The radioactive intensity of each spot was ... stress responses by regulating stomata opening and closing in Arabidopsis thaliana Plant J 2008, 55(3):455-466 50 Tripathi V, Parasuraman B, Laxmi A, Chattopadhyay D: CIPK6, a CBLinteracting protein ... ABA within d indicating its involvement in early responseto stress, whereas inthe later period of treatment, ABA content was re-adjusted and maintained Overall, ABA accumulation in PUSABGD72...
... about a Between-line variability: intraclass correlation The values ofthe coefficients of intraclass correlation forthe wing/thorax ratio are given in table IX Thera are no significant variations ... Standard deviations are much higher forthe wing than forthe thorax Forthea decrease inthe standard deviation is observed with increasing temperature, well as a lower value in males Some of these ... note a relative stability ofthe CV at intermediate temperatures and an increase at extreme temperatures, especially at 12 and 31°C = = Between-line variability and intraclass correlations Variation...
... critical for cell fate .96 xi ABBREVIATIONS ASNS asparagine synthase ATF activating transcription factor ATF3 activating transcription factor ATF4 activating transcription factor ATF5 activating ... yeast and ATF4 and ATF5 in mammals (5, 66) The upstream open reading frames (uORF) present inthe 5’-leader of these mRNAs regulate their translation inresponseto eIF2~P Inresponseto amino ... facilitates cytoplasmic splicing of mRNA encoding the Xbox binding protein1 (XBP1) in mammals and homologue ofthe mammalian activating transcription factor/cAMP -response element (CRE)-binding...
... TCV-CPHCRSV and HCRSVCPTCV, Arabidopsis thaliana, Hibiscus cannabinus and Nicotiana benthamiana were used Both A thaliana and H cannabinus were used as the starting material for isolating protoplasts All ... HCRSV and HCRSV-CPTCV could express CP in H.cannabinus protoplasts Figure 6.1 cRNA sample preparation from total RNA of Arabidopsis for microarray analysis Panel A: cDNA derived from total RNA samples ... that we can take advantage of abundant genetic information of this model plant Forthe research about interaction using viral mutants some results have already been reported An example was that...
... such as Pakistan that previously supported the Taliban Fearing instability, Pakistan offered information about, and access to, the Taliban and Afghanistan tothe United States In return, the United ... operates must changeThe goal, of course, is tochangeina manner that continually leads to an increased ability ofthe community to profitably satisfy customers THE CHOREOGRAPHER Let’s take a closer ... without a clear gain Today’s efficient businesses should use an iterative approach to discovering and satisfying the needs and wants of ❘ The Collaboration Imperative 11 their customers And today the...
... such as Pakistan that previously supported the Taliban Fearing instability, Pakistan offered information about, and access to, the Taliban and Afghanistan tothe United States In return, the United ... operates must changeThe goal, of course, is tochangeina manner that continually leads to an increased ability ofthe community to profitably satisfy customers THE CHOREOGRAPHER Let’s take a closer ... without a clear gain Today’s efficient businesses should use an iterative approach to discovering and satisfying the needs and wants of ❘ The Collaboration Imperative 11 their customers And today the...
... interpretation ofthe statistical analysis and helped to draft the manuscript All authors read and approved the final manuscript Competing interests The authors declare that they have no competing interests ... analysis was conducted using the baseline clinical and HRQoL data from 96 ofthe men and women inthe cohort from whom we had complete baseline data in order to determine the clinical validity ofthe ... baseline characteristics ofthe 112 men and women living with HIV/AIDS who were included inthe analysis The cohort was predominantly male with a mean age of 49.1 years (SD = 8.2) and Caucasian...
... plummeting valuations, and soaring layoffs Others have a sense ofthe association and are trying to see their way clear Not all ofthe economic malaise ofthe years 2000 to 2002 is due to these ... the balance of power has shifted tothe customer, companies are learning that they must collaborate Part One ❘ The Era of Collaborative Business with their customers and other businesses inthe ... interactions and depth of information you must build and maintain in your efforts to form successful collaborative relationships The value creation process inthe era of collaborative business begins and...