initiation stage centrioles duplication or multiplication and cell cycle regulation
Study of human nasal epithelial stem or progenitor cell growth and differentiation in an in vitro system
... a) Initiation stage: centrioles duplication or multiplication and cell cycle regulation In this stage, there are two important factors in the preparation of ciliogenesis: exist cell cycle and ... three main stages both in primary and motile cilia: i) Initiation stage: Cells exit the mitotic cycle after centrioles duplication or multiplication, ii) Early stage: Free centrioles for axoneme ... membrane, and form basal body, iii) Late stage: Elongate the ciliary axoneme 22 Figure 1.4 The stages of ciliogenesis After centrioles duplication or multiplication, the cells exit from normal cell cycle...
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Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
... transitions: twist in development and metastasis Cell 118, 277–279 31 Zipori D (2004) Mesenchymal stem cells: harnessing cell plasticity to tissue and organ repair Blood Cells Mol Dis 33, 211–215 32 ... induction Therefore, the enrichment of liver function genes in AT-MSC-Hepa was statistically significant In contrast, for example, the gene categories relating to cell cycle and organelle localization ... hepatic cells, the findings presented here suggest that MET might be a pivotal factor in determining stem cell transdifferentiation Discussion AT-MSCs may be good candidates as stem cells for cell...
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báo cáo hóa học:" BRCA1/2 genetic background-based therapeutic tailoring of human ovarian cancer: hope or reality?" ppt
... transcriptional regulation, plays a key role in regulation of cell death and survival and represents an important regulatory factor in the molecular events leading to the development of cancer or inflammatory ... breast tumor cases and 20-40% of ovarian tumors [5] In a 2003 report [6] the risk of breast and ovarian cancer for Ashkenazi women with inherited mutations in the tumor suppressor genes BRCA1 and BRCA2 ... of patients for genetic counseling and testing procedures, as early onset, multiple tumors especially in the breast, and family history for the same or other BRCA1/2 related tumors The increased...
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Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps
... epithelial cell populations before fractionation according to [4] Positive controls for invasive capacity were cell lines MCF7 and PC3M whose invasion score was 18-36%, whereas normal cell lines PNT2 and ... data analysis and design SL performed the stem cell isolations from benign samples PB performed stem cell isolations from tumors CH performed culturing and isolation of stem cells, and NF-κB experiments ... proteins were also expressed by the majority of progenitor and stem cells from tumor cell cultures (Figure 4c,d), whereas only a minority of the total cell population expressed PAPPA (Figure...
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Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx
... [6,12] Therefore the GroELS genes were overexpressed with those for each of the MCAD mutants, K364R and R256T, and for the wild-type enzyme, in E coli cells grown both at 31 °C and 37 °C, and the ... Buckinghamshire, UK) Filters for the Amicon Centricon device were purchased from Millipore Ireland BV (Cork, Ireland) Mutagenesis and subcloning of R256T and K364R The genes for the mutant MCAD proteins ... vector, using the EcoRI and HindIII sites The expression vectors were sequenced across the region encoding the MCAD gene, to exclude PCR-based errors Each mutant MCAD vector was cotransformed...
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Báo cáo y học: " Inhibition of constitutively active Jak-Stat pathway suppresses cell growth of human T-cell leukemia virus type 1-infected T-cell lines and primary adult T-cell leukemia cells" potx
... necessary for cell proliferation and differentiation [8] Abnormal activation of Stat proteins is a common characteristic found in various human tumor cell lines and human tumors including leukemia and ... AG490 reduces cell growth of HTLV-1-infected T -cell lines and primary ATL cells (A) HTLV-1-infected T -cell lines (5 × 104/mL) were treated with 0, 25 or 50 µM AG490 for 24 or 48 h Cell numbers ... of Stat3 and Stat5 in HTLV-1-infected T -cell lines, we treated the cells with 50 µM AG490 for 24 h and performed EMSA (Figure 3F) AG490 decreased SIE- [MT-2, HUT-102 and ED-40515(-)] and β-casein-...
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Báo cáo y học: "nalysis of the mouse embryonic stem cell regulatory networks obtained by ChIP-chip and ChIP-PET" pptx
... transcription factors and regulators of gene expression, which are important in development For instance, the expanded OCT4 and NANOG regulatory network contained genes such as Hoxa1, Foxd3, Msx2 and Hexb, ... be important to gain a thorough understanding of the means by which a stem cell maintains its identity, and how it can be directed to form different cell types Our work will allow for a more detailed ... expand our current knowledge of the mouse ES cell regulatory network that is orchestrated by the transcription factors OCT4 and NANOG Although both the ChIP-chip and ChIP-PET technology platforms...
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The impact of human resource management practices on employee's job satisfaction in Vector Aviation Co., Ltd
... produce information for use in recruitment and selection, performance management, evaluation for training and development Therefore, job analysis takes an important role in HRM According to McCormick ... (Scarborough and Elias 2002) On the other hand, the poor recruitment choice can have undesirable result for organization such as higher rate of turnover, reduced performance effectiveness and work ... individual learner (or team) And he or she will be encouraged to take more responsibility for his or her learning Efforts will be made to develop a climate that supports effective and appropriate...
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ASSESSMENT OF THE BENEFITS OF EXTENDING THE TROPICAL RAINFALL MEASURING MISSION A PERSPECTIVE FROM THE RESEARCH AND OPERATIONS COMMUNITIES doc
... International Precipitation Working Group (IPWG) is cosponsored by the Coordination Group for Meteorological Satellites and the World Meteorological Organization The second IPWG workshop was held in Monterey, ... used internationally for operations by World Meteorological Organization centers throughout the tropics for monitoring and forecasting tropical cyclone activity Groups in Japan and Europe have begun ... Another year of TMI and PR data for tropical storm monitoring and forecasting** • Another year of TMI data for numerical weather prediction** • Another year of PR and TMI data for enhancing near-real-time...
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Báo cáo lâm nghiệp: "Discrimination of coated carbide tools wear by the features extracted from parallel force and noise level" potx
... between parallel force and clearance wear, and noise level and clearance wear for all cutting speeds performed For this discussion, the parallel force and noise level behaviors of the TiN/AlN ... experimented for cutting wood based materials, and their wear characteristics were reported [3–5] In an effort to provide more information, the parallel force and noise level of the tools, and their ... wear Regression equation and its correlation coefficient for the linears in Figures and are summarized in Table IV The regression equations and correlation coefficients for the other tools are...
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Báo cáo y học: " Early detection of Varicella-Zoster Virus (VZV)-specific T-cells before seroconversion in primary varicella infection: case report" potx
... Varicella-Zoster Virus (VZV)-specific T-cells before seroconversion in primary varicella infection: case report Virology Journal 2010 7:54 Acknowledgements Financial support by the Deutsche Forschungsgemeinschaft ... 2035/3-1 and SPP1230 priority program “Mechanisms of gene vector entry and persistence”) to AB, and the Bundesministerium fuer Bildung und Forschung (BMBF BioChancePLUS/FKZ: 0315182) to AB, HN, and ... circumstances, as for example in cases of atypical or subclinical varicella or in the absence of detectable VZV DNA in plasma Consent Written informed consent was obtained from the patient for publication...
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Báo cáo y học: " Identification of super-infected Aedes triseriatus mosquitoes collected as eggs from the field and partial characterization of the infecting La Crosse viruses" pot
... fragments, and the L segment in four fragments (Table 6) PCR was performed in a thermal cycler with the following program: 94°C for min, 37 cycles of [94°C for min., 57°C for and 72°C for 2.5 min] ... cycling conditions for the RT-PCR protocol were: 55°C for 30 min, 94°C for min, 40 cycles of [94°C for 30 s, 56°C for 30 s, and 68°C for min] followed by a final extension at 68°C for RT-PCR products ... Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523-1692, USA, 2Current address: Colorado Department of Public Health and...
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Báo cáo y học: " Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury" pps
... staining, cells were washed in EBM-2 supplemented with 10% FCS Efficiacy of coloration and cell morphology was checked by fluorescence microscopy twice before transplantation Furthermore, growth, morphology ... that cell group in forward and side scatter is shown in Panel A as the green cell group Panel A and B contain furthermore as a red group a standard dose of control beads in order to quantify cells ... integrity, be it as vector for mediators as already shown with bonemarrow derived cells for the pulmonary epithelium [97] or as a "Trojan horse" aiming at tumor vessels for therapeutic interventions...
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Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx
... donors and recipients Mice were purchased from Jackson Laboratory (Bar Harbor, ME) and maintained on a standard laboratory diet and housed in a controlled environment with a 12-hour light/dark cycle ... in donor cells A representative image and an emission scan of QDlabeled donor cells are shown in Figure 1A and 1B Fluorescent particles were detected within the cytoplasm of donor cells and the ... Fort Collins, CO) Typically, 200,000 events for MNCs and 400,000 events for BMCs were acquired and the frequency of QD-positive cells was measured The numbers of donor cells in circulation and...
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Báo cáo y học: " A comparative study of a theoretical neural net model with MEG data from epileptic patients and normal individuals" docx
... − h) and m = αnµ − h The distribution of en+1 is therefore also normal, since the probabilities for l and m are mutually independent, and its variance is the sum of the variances of l and m ... standard EEG recording positions as its origin; in this study we used the P3, P4, T3, T4, F3, and F4 recording positions [14-16] Around the origin (T3 or T4 for temporal lobes) a rectangular 32-point ... the forward and inverse problems and discussed some new approaches Especially, he described the forward problem for both homogenous and inhomogenous media He referred to the Geselowitz's formulae...
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Báo cáo y học: " Effect of neutrophil elastase and its inhibitor EPI-hNE4 on transepithelial sodium transport across normal and cystic fibrosis human nasal epithelial cells" pot
... amphotericin B and 100 mg/ml of gentamicin) and transported to the laboratory for cell isolation Briefly, NP samples were rinsed in phosphate-buffered saline (PBS) with dithiothreitol (5 nM) and antibiotics ... compartment and Isc was monitored for 30 to 60 before apical addition of 10 μM amiloride, a specific inhibitor of ENaC Amiloride-sensitive Isc was determined as the difference in current with and without ... hNE and its specific inhibitor EPI-hNE4 on transepithelial Na+ transport across cultured normal and CF HNEC Our results showed that neither hNE nor trypsin treatment did modify I sc in normal and...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... proportion of Grade A, B and C colonies were 94.5%, 3.0% and 2.5% respectively for 24h exposure to 4oC (n=200); and 97.5%, 1.0% and 1.5% respectively for 24h exposure to 25oC (n=200) The proportion ... sides with parafilm (for minimal evaporation), and placed in either a 4oC refrigerator, or back into the incubator reset at 25oC An atmosphere of 5% CO2 was maintained only for the physiological ... SSEA-3 and TRA-1-81; (2) Grade B that was partially differentiated, with some areas of nonuniform cell morphology and non-distinct boundaries but with still relatively strong expression of SSEA-3 and...
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Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
... dithiothreitol), incubated with or without mm of the respective nucleotide or phosphate compound for h at °C, and stored for at least weeks at )80 °C before use for kinetic and molecular mass analyses ... allowed to stand at °C for h, and then stored at )80 °C for at least weeks before gel filtration This treatment did not affect the enzyme activity, as the same Vmax was obtained before and after ... exhibited low or high catalytic efficiency Therefore, the various incubated hTK1 forms from Figs and were analyzed for their kinetic behavior with dThd, and the results are presented in Figs and Only...
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Tài liệu Báo cáo khoa học: Transcriptional upregulation of inflammatory cytokines in human intestinal epithelial cells following Vibrio cholerae infection pptx
... at · 100 MOI for different times (0, 5, 10, 15, 30, 60, 120 and 180 min), (C) LPS at lgÆmL)1 for 0, and h, (D) CT at 4.5 ngÆmL)1 for 0, 1, and h, and (E) CT at ngÆmL)1 for 0, 1, and h The problem ... 13-acetatestimulated and ionomycin-stimulated peripheral blood mononuclear cells for IL-2, IL-4, IL-5, IFN-c, and LPSstimulated peripheral blood mononuclear cells for IL-10, IL-12p40, ENA-78 and Caco-2 cells for ... transcription of many proinflammatory cytokines, cell surface receptors and adhesion molecules, and therefore thought to be important in the generation of acute inflammatory responses [15] The activities...
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