in vivo studies of brain oxygen metabolism and oxidative phosphorylation
Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... harvesting complex (LHCI), consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family LHCI is bound along one side of PSI only, in the vicinity of the PSI-F and PSI-J ... shown in Fig 4C Interestingly, both tags are recognized more efficiently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G in vivo In order to examine the topology of ... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding...
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Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis pot
... [27] In rats Fig Western blot analysis of p53 and c-Jun in liver and lung Total protein extracts were obtained as described in experimental procedures A single band of about 53 kDa was detected in ... liver and lung indicating that the amount of p53 protein was significantly increased in the liver and lung from vitamin A-deficient rats that in controls c-Jun protein was significantly decreased in ... cared and handled in conformence with the Guiding Principles for Research Involving Animals and Humans, approved by the Council of the American Physiological Soceity The School of Medicine Research...
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Báo cáo khoa học: " In vivo assessment of catheter positioning accuracy and prolonged irradiation time on liver tolerance dose after single-fraction 192 Ir high-dose-rate brachytherapy" pot
... number of dose-contributing catheters increases Therefore, the catheter contribution index increases with the number of contributing catheters In case of a single contributing catheter, IP = In case ... contribution index (Eq 2) shown in color coding In case of two equally contributing catheters, Di/Dtot = 0.5 and IP = 2.0 IP is always in the range between and indicates that the catheter contribution index ... 5.4 mm and of interstitial edema (T2-w images) -5 ± 6.0 mm in plane, indicating a shift of the irradiation effect volume against the corresponding tolerance dose volume in the direction of the...
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Báo cáo y học: " A remission spectroscopy system for in vivo monitoring of hemoglobin oxygen saturation in murine hepatic sinusoids, in early systemic inflammation" doc
... detected an increasing NAD(P)H autofluorescence representing an intracellular inadequate oxygen supply Both changes are accompanied by increasing markers of liver cell injury Future therapeutic interventions ... disturbances in oxygen supply and those of oxygen utilization Conclusions There is a significant reduction in hepatic sinusoidal HbsO2 during the early stages of systemic inflammation In parallel, ... that the intravenous infusion of a special oxygen quenching dye allowed the visualization of the oxygen distribution on the liver surface using intravital videomicroscopy The fluorescence of the...
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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
... CPS and O-chain LPS are composed of linear trisaccharide repeating units containing 3-linked 2-acetamido-2-deoxy-D-quinovose, 4-linked 3-[(N-acetyl-L-alanyl) amido]-3-deoxy-D-quinovose and 3-linked ... O-chain containing L-rhamnose, 2-amino-2-deoxy-D-mannose and D-glucose [11], confirming that the novel CPS and O-chain polysaccharide were produced during the 72-h in vivo culture period In addition, ... (GalNAcAcontaining trisaccharide repeating unit) (Fig 4A, inset), based on this ratio about 40% of GalNAcA in both CPS and O-chain polysaccharides was present in the form of a primary amine When...
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báo cáo hóa học:" Functional significance of the signal transduction pathways Akt and Erk in ovarian follicles: in vitro and in vivo studies in cattle and sheep" ppt
... Effects of active immunization of sheep against an amino terminal peptide of the inhibin αC subunit on intrafollicular levels of activin A, inhibin A and follistatin Journal of Endocrinology ... in mediating the effects of FSH on inhibin-A, activin-A, oestradiol and progesterone secretion and also important in mediating IGF-I stimulated inhibin-A, activin-A, follistatin, oestradiol and ... for luteinizing hormone and its interaction with insulin: activation of Janus kinase/signal transducer and activator of transcription and phosphoinositol 3-kinase/Akt pathways Endocrinology 2003,...
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Báo cáo toán học: "OXIDATIVE PHOSPHORYLATION: Kinetic and Thermodynamic Correlation between Electron Flow, Proton Translocation, Oxygen Consumption and ATP Synthesis under Close to In Vivo Concentrations of Oxygen" docx
... reaction and the O2 electrode Fourth, to 10 μl of standard medium containing from to 400 nmols of ADP were added into the cell and the system again incubated until the O2 and ATP (contaminating the ... O2 and protein Thus, when the concentration of protein is increased maintaining constant the concentration of O2, the energy directly involved in the synthesis of ATP is substantially reduced Indeed, ... first seconds of the process of oxidative phosphorylation Each unit of O2 uptake in the y-axis represents 0.036 nmols of O for the additions of 0.92 and 2.76 nmols of O, and 0.197 nmols of O for the...
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In vitro and in vivo studies into the antidiabetic and antilipidemic effects of chlorogenic acid
... available in insulin-sensitive organs such as liver, kidney, muscle and adipose tissue Activation of insulin signaling upon binding of insulin to insulin receptors causes suppression of gluconeogenesis ... predominant insulin resistance with relative insulin deficiency or predominant insulin secretory defect with insulin resistance The etiology of this form of diabetes is wide and complicated, ranging ... quality of life Before the introduction of the therapeutic use of insulin, diet 11 is the main form of treatment of the disease, and dietary measures included the use of traditional medicines which...
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In vivo and in vitro studies of th17 response to specific immunotherapy in house dust mite induced allergic rhinitis patients
... between in vivo and in vitro findings, confirming that not only Th2, but also Th17 cells play an important role in the context of allergic inflammation IL10 producing CD4+ T cells showed a distinct ... treatment were used in the following in vivo and in vitro studies 3.3 In vitro Study To mimic the functional response of Th17 and other Th cell subpopulations to HDM allergens, an in vitro allergen ... changes of T-cell phenotype to the nasal mucosal inflammation following SIT therapy In conclusion, our in vivo and in vitro studies presented the evidence that not only Th2 but also Th17 mediated inflammation...
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Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier
... lung, and kidney were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo ... stability and toxicity of PANs, and further investigated the sustained release behavior in vivo of drug loading in PANs PA was firstly synthesized and characterized by FT-IR and 1H NMR, and then ... after i.v at a dose of 200 mg/kg In vivo pharmacokinetics The plasma levels of EPI were determined following a single i.v injection of EPI or PA/EPI (10 mg/kg EPI equiv.) in female Wistar rats...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this family are proteins of 142–144 amino-acids ... identified by other means as an in vivo substrate of GroEL [27] Interestingly, GatY is also included in a list of proteins that aggregate at 42 °C in E coli containing a dnaK deletion mutation (DnaK ... Department of the Weizmann Institute by a single injection of 150 lg of the purified protein, followed by two booster shots of the same amount of protein at 2-week intervals Serum was collected and used...
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Báo cáo khoa học: A study of microRNAs in silico and in vivo: bioimaging of microRNA biogenesis and regulation doc
... understanding of the production and function of miRNA in vivo as well as for monitoring the therapeutic potential of miRNAs in cancer Bioimaging of microRNA biogenesis The molecular mechanisms involved ... vector containing three tandem repeat regions of miRNA23 binding sequence (reprinted with permission [11]) information for understanding the miRNA biogenesis related to human diseases Imaging miRNA ... et al [22] obtained quantitative in vitro images of nine transcripts that were predicted to be miRNA9 targets using the ensemble machinelearning algorithm However, in vivo imaging of miRNA regulation...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... ratio of apparent dissociation constants for binding GST-IjBa in the presence and absence of ATP, and the value of a indicates whether the binding of one substrate (ATP) affects the afnity of the ... = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software measures the intensity of NF-jB in ... providing the recombinant IKK2 (rhIKK2) and Nandini Kishore for providing the SC-514 inhibitor We thank also Paul Hayter, Sasha Sreckovic and Matthew Strawbridge with help in growing and analyzing...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
... and NOS isozymes in rat brain and aorta The level of HSP90 and the type of NOS isoform present in rat brain and aorta were determined by immunoblotting (Fig 1) In brain, nNOS was the most preferentially ... al In vivo degradation of NOS and HSP90 by calpain B A C D Fig In vivo digestion of NOS and HSP90 in NMS rats during HSD treatment Aliquots (20 lg protein) of rat brain soluble material (A) and ... previously to induce an increase in the level of [Ca2+]i and a correlated calpain activation [28] This increase in [Ca2+]i is more intense in aorta than in brain Under these conditions, in brain, no...
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báo cáo hóa học: " Relationship between the EQ-5D index and measures of clinical outcomes in selected studies of cardiovascular interventions" pot
... variability in the variables of interest in these patients, and in particular, the ceiling effect may constrain the variation in EQ-5D index scores In the patients studied here, the EQ-5D index scores ... [33,34], and was assumed to be 0.05 in the primary analyses of many of the studies used here Changes in ETT and CCS that have been considered clinically important differences in many of the cardiac studies ... index for a ten year increase in age, males versus females, a one minute increase in ETT, a one class increase in CCS, a 10 unit increase in the SAQ scales and a 0.1 unit change in SF-6D as these...
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báo cáo hóa học:" Function of anterior talofibular and calcaneofibular ligaments during in-vivo motion of the ankle joint complex" docx
... height of 175 cm and average body mass index (BMI) of 21.8 kg/m2) were included in this study None of the participating subjects had subjective complaints of ankle pain, previous history of ankle injury ... inversion and internal rotation Strain in the CFL was found to increase in dorsiflexion and inversion Ozeki et al.[7] investigated strain changes in central fibers of the lateral ankle ligaments in ... numerous in- vitro studies which investigated the changes in ATFL and CFL strain in response to loading in various positions Colville et al.[10] measured strain in lateral ankle ligaments in 10 cadaveric...
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Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs doc
... small intestine (Huang and Adams 2004) The experimental results of Casula and Cutting (2002) suggest that B subtilis germinates in the jejunum and ileum The adverse conditions in the small intestine ... strain of Bacillus showing the greatest in vitro potential for its effectiveness in improving piglet performance and faecal flora Materials and methods Bacterial strains, growth media and initial ... the performance and faecal microflora of weanling pigs is shown in Tables and Over the 4-week feeding trial, all diets containing Bacillus supplements resulted in increased weight gain compared with...
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Probiotic Lactobacillus Strains: in vitro and in vivo Studies pot
... verification of their immunomodulating properties in in vivo mouse model MATERIAL AND METHODS Survival of Lactobacillus strains at low pH and in the presence of bile salt Lactobacillus strains isolated ... the mixture of bacteria was, therefore, used for in vivo experiment in mice During studies in mouse model the selected strains were safe and affected significantly the cytokine profile toward ... KALLIOMAKI M., KIRJAVAINEN P., EEROLA E., KERO P., SALMINEN S., ISOLAURI E.: Distinct patterns of neonatal gut microflora in infants in whom atopy was and was not developing, J.Allergy Clin.Immunol 107,...
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Báo cáo khoa học: "Comparative studies of the water relations and the hydraulic characteristics in Fraxinus excelsior, Acer pseudoplatanus and A. opalus trees under soil water contrasted conditions Damien Lemoinea, Jean-Paul Peltierb and Gérard" pdf
... study of diurnal regulation of the water status in Acer pseudoplatanus and Fraxinus excelsior trees growing in well-watered floodplains (site 1) The experiments were carried out in June 1999 and ... characteristics in F excelsior, A pseudoplatanus and A opalus trees 3.2 Regulation of water status in Fraxinus excelsior and Acer opalus trees growing in lowwatered floodplains (site 2) During these ... on expanding leaves determined in other years [10] In contrast to ash leaves, the leaf water potential of Acer pseudoplatanus showed low diurnal variations During the first part of the morning,...
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Báo cáo y học: "The in vivo expression of actin/salt-resistant hyperactive DNase I inhibits the development of anti-ssDNA and anti-histone autoantibodies in a murine model of systemic lupus erythematosus" ppt
... model of autoimmunity Transient autoimmunity can be induced in mice by the administration of a single high dose of LPS This induces a pulse of nucleosomes and chromatin in the plasma within the ... protein and the level of free G-actin in the circulation at sites of inflammation inhibiting the DNase I activity Our data obtained by a transgenic expression of the mutant protein (ash.DNase I) in ... identificantion of circulating DNA and renal localization of DNA-anti-DNA complexes J Exp Med 1977, 145:1115-1130 Fish F, Ziff M: The in vitro and in vivo induction of anti-doublestranded DNA antibodies in...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25