in vitro and in vivo applications
Báo cáo y học: "TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood" ppt
... reduction in SDF-1 production in increased OCP mobilization in vivo needs to be further confirmed using a model where SDF-1 concentration in the bone marrow is maintained in the presence of TNF Since ... NaN3 and 5% sucrose in PBS Culture supernatants were diluted in reagent diluent (1% bovine serum albumin in PBS) and incubated for hours at room temperature The detection antibody was diluted in ... downregulates SDF-1 mRNA expression in osteoblasts [17] TNF did not increase G-CSF in ST2 cells (data not shown), however, suggesting that the reduction in SDF-1 induced by TNF in vitro is not mediated by...
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Báo cáo y học: "Pro-inflammatory properties of stromal cell-derived factor-1 (CXCL12) in collagen-induced arthritis" pptx
... also points to a role for CXCL12 in positioning CXCR4+ T and B cells to distinct synovial microdomains as well as in retaining these cells within the inflamed synovial tissue [9] CXCL12 induces ... limb, as described in [17]: score 0, normal; score 1, redness and/ or swelling in one joint; score 2, redness and/ or swelling in more than one joint; score 3, redness and/ or swelling in the entire ... Fore and hind limbs (ankles and interphalanges) were fixed in 10% formalin and decalcified with formic acid Paraffin sections were haematoxylin stained Severity of arthritis was evaluated blindly...
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Báo cáo khoa học: Syndecan-4 is a signaling molecule for stromal cell-derived factor-1 (SDF-1)/ CXCL12 pptx
... heparitinases Finally, we asked whether HS and SD-4 were involved in other SDF-1-induced cellular activation signals As SDF-1 binding to CXCR4 activates p44 ⁄ p42 MAPK and JNK ⁄ SAP kinases and ... cells were cultured in DMEM (Invitrogen Corp., Paris, France) containing 10% fetal bovine serum (FBS; Biowhittaker, Paris, France) and l-glutamine (2 mm; Invitrogen Corp.), and split twice a week ... heparitinase I and III and chondroitinase ABC mixture, we demonstrate the strong GAG dependency of this binding However, our data not rule out the additional involvement of protein–protein interactions...
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Báo cáo sinh học: "Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" pot
... Fyn they can bind SH2 domains of other kinases, especially the zeta associated protein, ZAP-70 The ensuing events lead ultimately to transcription of genes, including interleukin-2, that promote ... events that follow ligand binding by the natural TCR, which involve clustering of CD28 (and perhaps other) molecules capable of providing co-stimulation into the same vicinity, the chimeric TCRs ... thank Hinrich Abken for the gift of a plasmid containing a cloned ScFv against CEA and Drs Abken and Zelig Eshhar for invaluable advice during the course of this project References 10 11 Before and...
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báo cáo hóa học:" Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" docx
... Fyn they can bind SH2 domains of other kinases, especially the zeta associated protein, ZAP-70 The ensuing events lead ultimately to transcription of genes, including interleukin-2, that promote ... events that follow ligand binding by the natural TCR, which involve clustering of CD28 (and perhaps other) molecules capable of providing co-stimulation into the same vicinity, the chimeric TCRs ... thank Hinrich Abken for the gift of a plasmid containing a cloned ScFv against CEA and Drs Abken and Zelig Eshhar for invaluable advice during the course of this project References 10 11 Before and...
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Báo cáo y học: "Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection" pptx
... of infectivity observed in the HUT78 cells, the HD cells showed increasing levels of p24 in the supernatants on both day-3 and day-5 post infection, suggesting a possible and crucial role in vivo ... according to functional categories including genes encoding for HIV-1 receptors and ligands (A), cell cycle and apopotosis (B), and cellular factors involved in HIV-1 infection (C) A fold change was ... for receptors, ligand binding, and cofactors, which are directly involved in HIV-1 infection, followed by analysis of changes in gene expression that occurs following HIV-1 infection Both HIV-1...
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Cloning of medaka glial cell derived neurotrophic factor (GDNF) and its receptor GFR alpha 1
... males and females, and the ratio of males to female kept in each tank is roughly : Embryos were collected in the morning and transferred into ERM (Embryo Rearing Medium) before incubating it ... alleged receptor in the brain This suggests the presence of another binding ligand to Gfrα1 in the medaka brain and also probably, a different role for Gdnf in the medaka fish (Clermont and Bustos-Obregon ... spermatogonia and Apr spermatogonia, in vivo (Meng et al 2000; Yomogida et al 2003) and in vitro (Nagano et al 2003; Kubota et al 2004; Hofmann et al 2005), hence establishing its central role in dictating...
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báo cáo hóa học:" Anti-tumor activity of patient-derived NK cells after cell-based immunotherapy – a case report" doc
... response against Hsp70-positive tumors within the first therapy cycles is in line with our previous findings from the phase I clinical trial In this study an intrinsic NK cell activity was initiated ... and directly before re-infusion Ex vivo TKD/IL-2-activated PBMNC were re-infused by intravenous (i.v.) injection within 30–60 using an infusion set consisting of syringe and a stem cell filter ... expression of perforin and granzyme B provided insight into the lytic activity of infiltrating T and NK cells In the primary tumor and in the anastomotic relapse there was a strong infiltration of...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... modulatory cytokines for groups of MDSC-inducing and non-inducing human cancer cell lines [16] These studies suggested multiple mechanisms of MDSC induction amongst tumor cell lines, including inflammatory ... GM-CSF Expression of ten putative MDSC-inducing factors was measured in MDSC-inducing (bold) and non-inducing HNSCC cell lines by qRT-PCR Increased CD33+ MDSC-induction capacity was associated with ... avenues, including inhibition of activation and expansion Using these and additional tumor cell line-induced MDSC samples (4-998 osteogenic sarcoma, DU 145 prostate carcinoma, CAKI-1 renal cell carcinoma,...
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Báo cáo sinh học: "Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model" potx
... potential in improving pulmonary functions in various settings of lung diseases, including acute lung injury [19-23] In addition to regulating angiogenic [24] and pro-inflammatory [25,26] cytokines ... (Figure 6, A and 6B), four acute inflammatory biomarkers, in group than those in groups and 3, and in group compared with those in group following acute lung IR injury In addition, the protein expression ... assay and troubleshooting SC, THT, MF, and SFK participated in data acquisition, analysis, and interpretation SL and HKY conceived of the study, and participated in its design and coordination and...
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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx
... hematoxylin and eosin, and then with combined Luxol fast blue and cresyl violet to identify myelin and nerve cells [10] Percentages of myelinated areas in damaged spinal cords were calculated using ... Alizarin red S D: Intense Alizarin red S stained after weeks incubation at osteogenic media and confirmed calcium deposition A and B: Oil red O stain, C and D: Alizarin red S stain, ×100 ASCs were ... effective in canine SCI model, there was little histological evidence of spinal cord tissue regeneration [22] In this study, we examined whether canine ASCs could survive and integrate into neural...
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Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx
... mixture into the joint In order to inject the stem cell and PRP mixture, she was first placed in a supine position with her right knee bent at 90° After cleaning with povodine-iodine and draping ... sedated with propofol mg intravenously (push) and a 20 mg/hour rate of continuous infusion After cleaning her abdominal area with povodineiodine and placing sterile drapes, an incision of approximately ... hip pain improved About a month prior to presentation, she again started having hip pain radiating to the anterior region of the right knee The pain was worse when standing up, walking, and exercising...
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Báo cáo y học: " Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells" pdf
... using the MACS (Miltenyi Biotec, Auburn, CA) magnetic affinity column according to the manufacturer's instructions In vitro induction of Th functions and intracellular cytokine staining The in ... LV, and MLV carrying a reporter gene After induction of maturation with LPS plus TNF-α, we harvested the DCs for antibody staining and FACS The results are shown in Fig 1C and summarized in Table ... retroviral infection induces upregulation of Th2 cytokines including IL-10 and impairs DC maturation [27,28] Because HIV causes immune suppression and the preceding results showed that LV infection...
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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
... et al and GFAP Mouse kidney and small intestine were used as control tissues for Ksp-cadherin or E-cadherin and pan-cyokeratin staining, respectively Sudan Red and Alizarin Red S stainings Sudan ... days in total), days after plating with puromycin (7 + days in total), 18 days after plating with puromycin (7 + 18 days in total) and 18 days after plating without puromycin (7 + 18 days in total) ... chromatin-associated protein that induces chromatin condensation and plays multiple roles in cellular processes, including transcription, DNA replication and repair [25] It is expressed early in...
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Báo cáo y học: "Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristic" doc
... that in in vitro assays, moDCs could bind and internalize certain antigens, particularly glycoproteins, more efficiently These findings are in keeping with those of Hajas et al [13] showing that ... expression in mDCs are known to be involved in cell growth and maintenance, including FGG, ubiquinol cytochrome c reductase, glutathione S transferase, nuclease isoform sm3 and annexin A1 Some ... for initiating immune responses and maintaining tolerance [1] DCs exist in two stages of maturation Immature cells are found throughout the body where they act as sentinels, continuously taking...
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Use of IPs cell derived neural stem cells as a cellular vehicle for glioma and breast cancer therapy
... Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden) CF01, U87MG-luci and C6 were maintained in DMEM supplemented with 10% FBS, Penicillin-Streptomycin and 5mM L-glutamine (Invitrogen) ... Sweden), maintained in DMEM supplemented with 15% FCS, 5% horse serum, penicillin-streptomycin, and NEAA Human embryonic stem cell line H1-derived MSCs and were generated and maintained using a method ... RNA interference (RNAi) targeting RNAi is a RNA dependent, gene silencing process that exerts its gene silencing effect through chromatin remodelling, blocking protein synthesis and cleaving...
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Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders
... al., 1996) The first DNA-binding domain used was a fruit fly homeobox domain, followed by zinc-finger DNA binding domain and yeast Gal4 DNA-binding 17 domain (Kim and Chandrasegaran, 1994; Kim ... were incubated with trypsin/EDTA (TE; Invitrogen) and trypsin inhibitor (TI; Invitrogen), mechanically triturated into single cells and plated on poly-l-ornithine/laminin (PO/Ln; both Sigma Aldrich) ... fusion proteins of a DNA-binding zinc finger domain and a double strand break-inducing nuclease domain Dimerization of nuclease domains is necessary for target cleavage The cellular machinery for...
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Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt
... The efficacy of infection for varying viral loads was determined by staining for b-Gal (data not shown) Metabolic [35S]methionine labeling and membrane preparation L6 cells were infected with hUCP3, ... three times in NaCl/Pi, then fixed in 4% (w/v) paraformaldehyde/NaCl/Pi for 60 The cells were washed four times in NaCl/Pi and then incubated for 60 in NaCl/Pi containing 0.1 M glycine, pH 8.6 ... permeabilized using 0.1% (v/v) Triton X-100 in NaCl/Pi for min, followed by four NaCl/Pi washes The slides were then incubated in blocking buffer containing 5% (v/v) fetal bovine serum, 0.1%...
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