... map onto ∆(ε) × ∆ In dimensions bigger than two the situation becomes more complicated; see Examples 3.2 and 3.3 in Section Let us give a condition on X sufficient to maintain the conclusion of ... eigenvalues By an appropriate version of the maximum principle for k-convex functions k-convexity implies disk-convexity in dimension k EXTENSION PROPERTIES OF MEROMORPHIC MAPPINGS 807 Condition (3) ... sphere CP1 in X under nonconstant holomorphic mappings Considering the space of rational cycles R(X) instead of Barlet space B1 (X) we can define as in Definition 1.5 the notion of bounded rational cycle...
... transition metal-PEO interaction In the case of synthesis mixture containing metal ions, the modified pathway can be now denoted as REFERENCES 554 A A M Daifullah, B S Girgis, H M H Gad Utilization ... structure directing agents in acid media via (S0H+)(X-I+) synthesis route where S0 is nonionic surfactant, H+ proton, X- acid anion, and I+ protonated silanol group C C CONCLUSIONS O O C C Synthesis ... direct mesostructure formation Futhermore, since the structure directing effect is irrespective of the anions used in the synthesis, and only the Cl- anion resulted in a branched, networklike...
... definition does not depend on the choice of desingularization In other words, f ∗ α is the trivial extension, as current, of (f|X\If )∗ α through If This definition induces a linear action on the ... Replacing S by S + Cω k−1 if necessary, we can assume ≤ S ≤ Cω k−1 for some constant C > We now take the pull-back of the previous equation by f , as explained in Section 1597 RATIONAL MAPPINGS ... case This result heavily relies on the following lemma We thank Julien Duval for explaining to us the construction of inverse branches on balls RATIONAL MAPPINGS WITH LARGE TOPOLOGICAL DEGREE...
... complex concentration up to the saturation, thus allowing for determination of ligand concentration, but not for stability constant calculations This behaviour is indicative of a higher binding constant, ... MH-2L, differing by the protonation state of the lysine amine, which is not involved in coordination For VIHN only the 4N species were detected, while potentiometric titrations indicated the ... constants are presented in Table An analogous titration was performed for Ni(II) complexation by DAHKam, in 100 mM phosphate, pH 7.4, using absorption spectra This titration yielded a linear increase...
... decreased considerably during incubation with cAK at 37 °C we used phosphomimetic point mutations at positions 117 and 162 to evaluate the effect of phosphorylation (Table 1) Single-point mutations ... PDE11A4, and it is conceivable that they have a function in conjunction with the regulation of PDE activity in addition to the phosphorylations in PDE5 and PDE11A4 (see above) [27] Indeed, we have ... compilation ª 2008 FEBS M Gross-Langenhoff et al Germany) was added after stopping the reaction to determine yield during product isolation The reaction was started by addition of ATP Substrate conversion...
... definition of p(k) by allowing k to be also in nity, with the corresponding parameter |E(G(l) )| p(∞) = sup lim inf , l→∞ l2 G(∞) where the supremum is taken over all graphs G(∞) without in nite increasing ... The following was stated in [1] as a question and in [2, 3] as a conjecture Conjecture 1.1 ([1, 2, 3]) For any k ≥ the following holds p(k) = 1− k (1) In this paper we will show that in general ... 1)2m+1 (2m+1 − 1) k(k − 1) + 4i(i − 1) 4m + o(4m ) = By Proposition 2.1 and equation (2) we obtain the following the electronic journal of combinatorics 15 (2008), #R47 Corollary 2.2 For any i ∈...
... the sub-domains was performed additionally in order to explore the relationships between the sub-domains Factor analyses using the varimax rotation on the 76 items with solutions allowing or 12 ... Satisfaction SH Satisfaction SH Satisfaction PC Comfort SS SS 0.54 0.41 0.31 0.31 0.52 0.44 Satisfaction 0.50 0.33 Satisfaction 0.51 0.40 SS Satisfaction 0.50 SS Satisfaction 0.48 10 SH Satisfaction ... respective domain are reported in the following The highest baseline correlation in the Satisfaction domain was r = 0.743 and the smallest was r = 0.512 Correlations in the Comfort domain were between...
... produced by A fumigatus This may be biologically relevant because inhibition of the proteases secreted by A fumigatus conidia during germination in lung tissues may severely limit the colonization ... corresponding to unbound fractions (Unb) or elutions with 0.15, 0.3 and M NaCl were loaded on a high-resolution SDS ⁄ PAGE gel and analyzed by western blotting using polyclonal anti-trappin-2 sera ... active in the range pH 2.0–7.0 Trappin-2 did not inhibit fibronectin degradation by C albicans proteases, which is essentially performed by acid proteases (data not shown) Scanning electron microscopy...
... probable that the kinetically in uential ionization observed in pH-dependent studies with benzylamine is attributable to the ionization of Asp128b Two ionizations were also identified in the plot of ... half-reaction of AADH as a function of PES concentration AADH was stoichiometrically reduced with benzylamine and rapidly mixed with different concentrations of PES under anaerobic conditions Conditions: ... consistent with TTQ reduction being partially rate limiting in steady -state turnover Our studies of the pH dependence of TTQ reduction by benzylamine have indicated that a single kinetically in uential...
... assists in the assembly of the proper interactions in the active site by stabilizing the closed conformation of the protein In the closed conformation, R120 moves from an ÔoutsideÕ position into ... amino acid side chains, R397 and R120, are also involved in fosfomycin binding, and in view of the multitude of interactions, it was assumed that deletion of a single interaction would not lead ... important residue in the stabilization of the closed conformation of the protein This hypothesis can be tested by assessing the extent of the conformational change occurring upon addition of UDPNAG...
... separately in the solution by manipulation of the temperature controller The change in morphology of a single ice crystal into a hexagonal bipyramid caused by the accumulation of AFP on the ice ... solution was placed in the cooling pot ()7 °C) of the instrument; (b) the frosty probe was put manually into the supercooled sample to initiate water freezing; (c) the increase in solution temperature ... has considerably increased TH activity compared with the monomer [27] In addition, for type I AFP isoforms from winter flounder consisting of 11 amino acid repeats, a longer isoform consisting...
... action [20] Besides acting by destabilizing membrane structures and changing ion permeabilities, pore-forming peptides can in uence cell functioning by interacting with intracellular signaling Interaction ... Pseudomonas aeruginosa ATCC 27853 Determination of minimal inhibitory concentration The bacteria were grown in Brain Heart Infusion (Oxoid, CM225) at 37 °C and after h, the suspension was diluted in ... Only fraction B was biologically active (C) Fraction B from the second purification was again loaded on the Xterra RP18 column using the same linear gradient Inhibition of superoxide production...
... present a good conversion in the oxidation reaction of glucose The conversion of the reaction reached 87.67% when using HNO3 as oxidizing agent, but the selectivity of D-gluconic product is quite ... reaction was about 2.7 This condition facilitated the formation of gluconolactone and disaccharide and inhibited the catalyst activity [2] So, we should add alkaline solution continuously to maintain ... oxidation on PS-1 catalyst On the other hand, the reaction on Pt/SBA15 catalysts with pH control not only showed a high conversion, but also high D-gluconic acid selectivity This determined a...
... and two ATP-binding domains referred to as the D1 and D2 domains Interestingly, deletion of the N-domain from ClpA not only abolishes binding of the adaptor protein, ClpS, but additionally modulates ... double point mutations within the N-domain to probe N-domain function Here, using mutational analysis, we report the identification of a structural element composed of conserved basic amino acids (R90 ... al Substrate recognition and unfolding by ClpA Fig Mutations in the N-domain not prevent substrate interaction (A) FITC-casein (500 pmol) was separated by gel filtration in the presence of mM...
... formation To test this assumption, fibril formation was investigated in the presence of increasing concentrations of fragmented N-(+7)Ala fibrils acting as seeds Clearly, an increase in seed concentration ... after preparation to protein solutions Quantification of soluble (monomeric) protein during fibril formation by RP-HPLC Samples were diluted : 25 with water to a final protein concentration of 0.5 mgÆmL)1 ... formation upon addition of seeds Fibrillation in the presence of different concentrations of N-(+7)Ala seeds (seeding) and N-WT seeds (cross-seeding) Rate constants were calculated by assuming...
... NaCl/Pi and incubated in inositol-free medium containing 10 mM LiCl for 30 min, after which AVP was added at the concentrations indicated for a further 30 Incubations were terminated by adding 0.5 ... restriction site (bold) The PCR cycling conditions were as follows: denaturing, 94 °C (1 min); annealing, 60 °C (2 min); extension, 72 °C (1 min) for 30 cycles followed by extension at 72 °C (7 min) ... arginyl at this locus is a constraining residue which contributes to maintaining the conformational switch of the V1aR in the off -state The unique ability of arginyl to fulfil this constraining...
... conditions imply that chemical reaction of AfM1PDH in the reduction direction proceeds much faster than the corresponding chemical reaction in the oxidation direction Under physiological pH conditions, ... different concentrations of inhibitor The observed inhibition was, in each case, best described by competitive binding of coenzyme and adenine nucleotide Inhibition constants (KiEI) were obtained from ... dehydrogenase acting in the direction of NAD+-dependent alcohol oxidation (see later) Inhibition by ketose substrate during NADH-dependent reduction of Fru and its absence under conditions in which NADD...
... ¼ 70 min) In contrast, the rate of inhibition was significantly increased in the presence of NaTDC monomers (T50% ¼ 16 min) At NaTDC concentrations beyond the critical micellar concentration, the ... concentrations beyond the critical micellar concentration having a higher efficiency than the monomer concentration (T50% ¼ versus T50% ¼ 30 min) The addition of colipase alone had a slight in uence ... proteins was independent of the nature of the C-terminal domain, in contrast to the situation with Nc proteins Indeed, the C2 domain induced sensitivity of the Nc active site to E600 inhibition in...