immunofluorescent staining for the detection of the hepatitis b core antigen in frozen liver sections of human liver chimeric mice
Methods in molecular biology vol 1540 hepatitis b virus methods and protocols
... Stefan F Wieland 11 Immunofluorescent < /b> Staining < /b> for the Detection < /b> of the Hepatitis < /b> B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice Lena Allweiss, Marc Lütgehetmann, ... against HBV core antigen (HBcAg), 1C10, 1 mg/mL; mouse mAb specific to HBV preS1 protein 2D3, 1 mg/mL; mouse mAb specific to HBV preS2 protein, 1F4, 1 mg/mL; mouse mAb specific to HBV S protein (HBsAg), ... permeabilized with 0.5 % Triton X100 for 10 min, and then were stained with 1C10 (a: HBV core) , 2D3 (b: PreS1), 1F4 (c: PreS2), or 1 7B9 (d: HBsAg) followed by Alex 488 conjugated rabbit anti-mice...
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Báo cáo y học: "Management of HBV Infection in Liver Transplantation Patients"
... for HBsAg and anti-HBs) has resulted in additional challenges, since isolated anti-HBc-positive livers are capable of transferring HBV infection to 50-78% of HBV-naïve recipients in the absence ... against HBV reinfection in patients receiving HBIG [85] and should no longer be used Hepatitis B Immune Globulin and Adefovir Controlled trials of this combination therapy have not been reported, but ... have cleared HBV from liver and plasma, and once reinfection occurs, HBIG cannot be reinstituted Indeed, HBV DNA has been detected in the liver, serum or leukocytes in 50% of HBsAg-negative patients...
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Tài liệu Báo cáo khoa học: "Automatic Detection of Nonreferential It in Spoken Multi-Party Dialog" doc
... verb that it is the subject of subj verb prep preposition before indirect object indirect object of verb that it is subject of ind obj obj direct object of verb that it is subject of obj verb ... reliably this kind of annotation could be done The annotators were asked to label instances of it in five ICSI Meeting Corpus dialogs1 as belonging 51 Bed017, Bmr001, Bns003, Bro004, and Bro005 ... Syntactic Patterns INF it it it was easy it BE adj it BE obj it’s a simple question it MOD-VERBS INF obj it’ll take some more time it VERBS TO-INF it seems to be Lexical Features noun complement (in...
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Detection of Pseudomonas aeruginosa in sputum headspace through volatile organic compound analysis potx
... produced by bacteria P aeruginosa may be detected by analyzing VOCs produced in vitro (Table 1), although the many studies addressing this question measured a variable range of VOCs Breath or Table ... JM, Bradley J, Mccaughan J, O’Connor TM, Shortt C, Bredin CP, Bradbury I, Elborn JS: Predictors of mortality in adults with cystic fibrosis Pediatr Pulmonol 2007, 42:525–532 10 Burns JL, Gibson ... Murdoch DR: Detection of volatile metabolites produced by bacterial growth in blood culture media by selected ion flow tube mass spectrometry (SIFT-MS) J Microbiol Methods 2006, 65:361–365 22 Carroll...
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R"eCseoarcuh garthicl eofficer screening" improves detection of pulmonary tuberculosis in hospital in-patients pptx
... institutionalized TB reporting and DOTS alone may be insufficient to achieve targets established by Stop TB In particular, previous studies have shown that TB diagnosis can be very complicated ... having TB, but only ~1% of COS (+) patients were eventually diagnosed as having TB (based on Mycobacterial cultures) Acute cough is a complication of many diseases [20], so our Lin et al BMC Public ... for diagnosis Indian J Tuberc 2008, 55:77-83 15 Banda HT, Harries AD, Welby S, Boeree MJ, Wirima JJ, Subramanyam VR, Maher D, Nunn PA: Prevalence of tuberculosis in TB suspects with short duration...
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ag doped wo3-based powder sensor for the detection of no gas in air
... low but clearly observable diffraction hump at the 2y angle between 10 and 188 in the 1% Ag/WO3 sample (Fig 5) Such low angles not match with any possible diffraction peaks from silver or possible ... 71 increased by three times Without heating the Ag catalyst or bypassing the gas stream from the Ag catalyst bed no sensitivity enhancement was encountered Similar observation was obtained when ... gas composition, ¯ow, etc can be reliably made by using the same sensor [16] As seen from Table 2, the optimum sensitivity for 40 ppm NO in air using our pure WO3 is about S Æ collected at 300...
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Báo cáo " On the detection of gross errors in digital terrain model source data " pdf
... estimate for the height value of the point Pi By comparing the measured value of Pi with the representative value estimated from the neighbors, a difference Vi in height can be obtained: Vi = H imeas ... Project area Height of surface / Std. deviation Number of data points Spatial distribution of data points Average distance between data points Number of data points with intentional gross error ... minimum number of points inside the search window), the number of correctly and incorrectly detected points is decreasing. This can be explained as a large number of points ...
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The bleach method improves the detection of pulmonary tuberculosis in Laos ppt
... site, on bloodcontaining specimens and when delays before analysis were 10 AFB/HPF) smears (Table 1) Positivity rates were higher using the bleach method, regardless...
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Báo cáo hóa học: " Detection of epithelial apoptosis in pelvic ileal pouches for ulcerative colitis and familial adenomatous polyposis" pot
... A-Sepharose MB was from Pharmacia (Uppsala, Sweden), and nitrocellulose paper (BA85, 0.2 μm) was from Amersham (Aylesbury, UK) The anti-Bax (sc-493, rabbit polyclonal), anti-Bcl-2 (sc492, rabbit polyclonal), ... anti-FADD (sc-5559, rabbit polyclonal), anti-Caspase-8 (sc-7890, rabbit polyclonal), antiAPAF-1 (sc 26685, goat polyclonal) and anti-Caspase-9 (sc-7885, rabbit polyclonal) antibodies were purchased ... the sections were microwaved in 3% milk buffer for 30 and incubated overnight with primary antibody either to Bax or Bcl-2 (DAKO A/S Denmark; A3533, rabbit polyclonal and M0887, mouse polyclonal)...
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Báo cáo khoa học: "Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR" pot
... diagnostic specimens by PCR J Clin Microbiol 1994, 32, 1497-1502 Gebhart, C J., S M Barns, S M McOrist, G F Lin, and G H K Lawson Ileal symbiont intracellualris, an obligate intracellular bacterium of ... negative by the PCR analysis, but the respective mucosal specimen was positive The result might be explained due to the sensitivity differences between sources, storage of feces at -80oC, subsequent ... with the fecal samples, but only 76.4% (13/17) by the conventional examination based on the microscopic observation These results indicated that the one step amplification by PCR reaction was more...
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Báo cáo khoa học: "Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections" pdf
... hourshybridization protocol Digoxigenin-labeled probe was capable of detecting CHV in MDCK cells infected with 103 TCID50 using overnight hybridization protocol However, biotin-labeled probe was able ... digoxigenin-labeled DNA probes MDCK cells inoculated with 103 TCID50 CHV Some signals (arrows) are observed NBT/BCIP colorization, methyl green counterstain, Bar = 50 µm Fig Overnight hybridization with biotin-labeled ... proteinase K pre-treatment, and overnight hybridization The practicality of digoxigenin-labeled probe is better than those of biotin-labeled probe in the hybridization assay for the detection of CHV...
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Báo cáo khoa học: "Time of initial detection of fetal and extra-fetal structures by ultrasonographic examination in Miniature Schnauzer bitches" ppsx
... erofeb detceted tsrif erew dub bmil dna taebtraeh ,oyrbme eht fo epahs ralopib eht ,yduts siht nI daeh eht ni aera ciohcena eht dna taebtraeh eht fo noitom gnirekcilf eht erew oyrbme ralopib eht ... cinegohcena eht ,1 elbaT ni nwohs sA serutcurts latef-artxe fo noitceted laitini fo emit ehT arecsiv lanimodba dna ,tnemevom latef ,noteleks ,sdub bmil ,taebtraeh ,oyrbme ,enarbmem latef ,atnecalp ... elbahsiugnitsid ylno eht ,03 yad erofeB 03 yad erofeb oyrbme eht nihtiw noitaitnereffid cimotana elttil detroper ]02[ regaeY ]02,11,9,8[ ylevitcepser ,taebtraeh dna oyrbme na fo gnidnif eht yb...
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Báo cáo khoa học: "Use of soft X-radiography for early non-destructive detection of floral differentiation in Douglas fir buds" pptx
... of the buds that could be analysed increased overall with time as did the size of the buds, from a small percentage on August (2-30) to almost the total number of buds on September (table II) ... the base of the bud Parallel observations of histologic sections of homologous buds (fig 7) showed that there was xylem vascularization in the shoot and procambium with proto-xylem at the base ... delimiting a ’chamber’ at the base of the buds, which quickly changed in shape and size under floral buds Whether the floral bud was male or female, the structure of the chamber was the same but its development...
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Báo cáo y học: "Conventional radiography requires a MRI-estimated bone volume loss of 20% to 30% to allow certain detection of bone erosions in rheumatoid arthritis metacarpophalangeal joints" ppsx
... imaging score applied to different joint combinations and the sharp/van der heijde radiographic score Arthritis Rheum 2005, 52:2300-2306 11 Perry D, Stewart N, Benton N, Robinson E, Yeoman S, Crabbe ... recommendations [4] By this method, erosions are scored on a scale from to 10 based on the proportion of eroded bone compared to the 'assessed bone volume' judged on all available images: = no erosion; ... reader (BE) has previously demonstrated a high inter- and intra-observer reliability [10,16] Overall, the results of the cited studies suggest that MRI observations of erosions are true and reliable...
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Báo cáo khoa học: "Detection of somatostatin receptors in human osteosarcoma" pot
... research papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately upon acceptance cited in PubMed and archived on PubMed Central yours ... possible relation between the somatostatin receptors presence and the biological behaviour of the tumour [30] References The authors declare that they have no competing interests Authors' contributions ... Kwekkeboom DJ, Avezaat CJ, Lamberts SW, Reubi JC: Somatostatin inhibits the activity of adenylate cyclase in cultured human meningioma cells and stimulates their growth J Clin Endocrinol Metab 1992,...
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Báo cáo y học: "Detection of bone erosions in rheumatoid arthritis wrist joints with magnetic resonance imaging, computed tomography and radiography" ppt
... visible in two planes with a cortical break seen in at least one plane [15] MRI bone erosions were scored according to the OMERACT RAMRIS; that is, all wrist bones were assigned a score by the ... reading B, volumes obtained at the first (reading A) and second (reading B) volume measurements, done by the same observer week apart The mean value of volumes obtained at reading A and B was used ... Studies on volumetric quantification of bone erosion volumes with MRI have previously shown it is a reliable and feasible method [19-21], and it could possibly be beneficial in documenting progression...
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Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps
... in humans Aquabacterium commune Aminobacter aminovorans (1 UA) [EMBL:AJ011759] 1344 98.72 Bacillus niacini (1 UA) [EMBL:AB021194] 1400 99.14 Bradyrhizobium japonicum (1 UA) [EMBL:AB072418] 1200 ... Paenibacillus humicus, × Streptococcus mitis, × Achromobacter xylosoxidans, × Bradyrhizobium elkanii, × Bacteroidetes bacterium, × Flavobacterium mizutaii, × γ proteobacterium, × Propionibacterium ... ReA) [EMBL:AJ429239] 1340 97.82 Uncultured bacteria Bacteroidetes bacterium (2 UA + ReA) [EMBL:AY395022] 1394 96.11* Bacteroidetes bacterium (1 ReA) [EMBL:DQ195837] 1300 98.39 β Proteobacterium...
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báo cáo khoa học: "Electrophoretic detection of interspecific hybrids in Parnassius (Lepidoptera Papilionidae)" docx
... In the laboratory, second generation backcrosses (Fl male hybrids x apollo females) have been obtained They displayed some developmental perturbation, but a significant number of viable, fertile ... hybrids have been regularly observed between 1970 and 1986 Their frequency has been roughly estimated to vary between and 10 % of the total Parnassius population in different years In the Bor6on ... region hybridization has been investigated for fewer years, but hybrids have been also observed regularly and perhaps with a higher frequency A female preserved in the collection of the Laboratory...
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Báo cáo y học: "Effective detection of rare variants in pooled DNA samples using Cross-pool tailcurve analysis" ppsx
... here Blue bar is 95th percentile score cutoff, discounting variants with max score Panel A SNP quality scores derived from Illumina base calls Panel B SNP quality scores derived from SRFIM base ... Muzny D, Albert TJ, Gibbs RA: Whole Exome Capture in Solution with 3Gbp of Data Genome Biology 2010, 11:R62 10 Okou DT, Steinberg KM, Middle C, Cutler DJ, Albert TJ, Zwick ME: Microarraybased Genomic ... Tucker EJ, Compton AG, Kirby DM, Crawford G, Burtt NP, Rivas M, Guiducci C, Bruno DL, Goldberger OA, Redman MC, Wiltshire E, Wilson CJ, Altshuler D, Gabriel SB, Daly MJ, Thorburn DR, Mootha VK: High-throughput,...
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báo cáo khoa học: "mRNA detection of individual cells with the single cell nanoprobe method compared with in situ hybridization" doc
... the nuclear membrane became greater, the probability decreased In the region 6–9 µm from the nuclear membrane, the probability was 61.5%, and in the region 9–18 µm away from the membrane, it was ... AFM has been applied for various biological samples because it can be operated in solution [15-19] An AFM probe is inserted into the living cells to extract mRNAs Obtained mRNAs are subjected ... method have been described in previous studies [12-14] Briefly, the AFM probe (NP, Digital Instruments, Santa Barbara, CA) was positioned onto a target region of cells under the observation of...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25