identification of human idiotype specific t cells in lymphoma and myeloma
Báo cáo y học: "Use of soluble MHC class II/peptide multimers to detect antigen-specific T cells in human disease." pps
... detection of in 1000 Studies have suggested that T cells with these specificities may be present at low frequency in the blood of rheumatoid arthritis patients, and it had been thought that the ... successfully to identify antigen -specific CD4+ T cells The intensity of staining correlates with the affinity of TCR for the particular MHC/peptide Although the frequency of antigen -specific CD4+ T cells ... If TCR expression is held constant, then the intensity of fluorescent staining with MHC/peptide multimers can be used as a measure of the affinity of the TCR for the MHC/peptide Binding of the...
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Báo cáo y học: "Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in Chlamydia-triggered reactive arthritis" docx
... receptors In comparison with intracellular cytokine staining, the major advantage of tetramer technology is the identification of antigen -specific T cells independently of their cytokine secretion profile, ... cells independently of their cytokine-secreting profile Tetramers are also capable of detecting resting antigenspecific T cells, which probably constitute most non-IFN-γsecreting CD8+ T cells of the ... MNCs and of the tetramer-sorted T cell line after weeks of culture In comparison with HLA-B27 tetramer staining, only 68.3% of these antigen -specific T cells were detected by intracellular cytokine...
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Báo cáo hóa học: " CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation" pptx
... results of the TUNEL assay are consistent with the tumor growth inhibition results Our observations indicate that the growth-inhibitory effect of Cdte QDs + DNR treatment is related to its ability ... illustrate that Cdte QDs with DNR treatment may indeed lead to improved selectivity toward leukemia cancer cells and facilitate inhibition of the proliferation of targeted cells Binding the positively ... treatment alone, the apoptosis rate increased after the treatment of Cdte QDs together with DNR in HepG2/ADM cells We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein...
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Báo cáo y học: "Heterogeneity of CD4+ and CD8+ memory T cells in localized and generalized Wegener’s granulomatosis." potx
... described Thus, the local cytokine milieu might depend on the site and extent of disease activity [4,5] The shift in the cytokine profile in granulomatous lesions of the respiratory tract might be of ... as response in vitro to chemotactic gradients, cytokine release and cytotoxic activity of distinct T cell populations Detection of the inducible inflammatory chemokine receptors CCR5 and CCR3 on ... steps mediated by selectin and adhesion molecules, chemokine receptors support the selective recruitment of differentiated T cells into tissues through interaction with endothelial and tissue-expressed...
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Báo cáo y học: "Skewed distribution of proinflammatory CD4+CD28null T cells in rheumatoid arthritis" docx
... preparation VM and CT were the principle investigators and participated equally in the planning and coordination of the study, interpretation of data, and drafting the manuscript All authors read and approved ... that in peripheral blood, as illustrated in Figure 4a–c These results suggest that the access of CD4+CD28null T cells to the joint is restricted to certain TCR-Vβ subsets, and that RA patients ... next investigated whether there was a selective recruitment of certain subsets of CD4+CD28null T cells to the joint Using antibodies detecting 24 different TCR-Vβ chains, we could identify TCR-Vβ...
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Báo cáo y học: "Indoleamine 2,3-dioxygenase-expressing dendritic cells are involved in the generation of CD4+CD25+ regulatory T cells in Peyer''''s patches in an orally tolerized, collagen-induced arthritis mouse model" ppt
... determining immunity or immune tolerance; this determination is based on the maturation or activation state and the subset of DCs, and cytokine profiles in the microenvironment at the time of antigen ... expression and inhibit T- cell proliferation In this regard, it will be interesting to investigate IDO expression in different subsets of DCs from tolerized mice and to characterize their role in the induction ... PCR using IDO sense (5'-CACTGTACCAGTGCAGTAG-3') and antisense (5'-ACCATTCACACACT CGTTAT-3') primers, and using Foxp3 sense (5'-CAGCTGCCTACAGTGCCCCTAG-3') and antisense (5'CATTTGCCACGAGTGGGTAG-3')...
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Báo cáo y học: " Heterogeneity of human effector CD4+ T cells" doc
... two sites in Th2 cells, but not in Th1 cells The collaboration of STAT-5 and GATA-3 accounts for full Th2 differentiation in vitro [33] Of note, there is a mutual regulation of Th1 and Th2 polarization ... killer cells upon stimulation by IL-12 activates the signal transducer and activator of transcription (STAT)-1 in the naive CD4 T cells Activated STAT-1 upregulates T- box expressed in T cells (Tbet) ... whether it acts directly or through the activation of ROR t is still unclear [52] The distinctive cytokine of murine Th17 cells, IL-17A, is involved in the recruitment, activation and migration...
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Báo cáo y học: "Identification of proteases employed by dendritic cells in the processing of protein purified derivative (PPD)" pdf
... Cathepsin H 0 Cathepsin A Cathepsin L Cathepsin J Cathepsin H Cathepsin D/E Cathepsin C Chymotrypsin BLT Esterase Cathepsin A Chymotrypsin BLT Esterase Cathepsin L Cathepsin J Cathepsin H Cathepsin ... [3-6,12] Thus, it will be interesting to compare DC from different tissues and in different states of maturation for their protease profiles and susceptibilities to pepstatin A treatment We believe that ... A inhibits cathepsin D/E activity relatively selectively [35] Thus, it appears that cathepsin D/E is the primary target of pepstatin A, with the implication that these proteases play important...
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Báo cáo hóa học: "IL-2 as a therapeutic target for the restoration of Foxp3+ regulatory T cell function in organ-specific autoimmunity: implications in pathophysiology and translation to human disease" doc
... [16,45] These results indicate that the lack of Treg cells in IL-2-/- and IL-2R-/- mice contributes to the autoimmune phenotype and that IL-2 maintains self tolerance by increasing the number of Treg ... homeostasis of CD4+Foxp3+ Treg cells in the target organ IL-2 restores the Treg/Teff balance in T1 D IL-2 is important in instructing Treg lineage commitment Apart from thymic-derived Treg cells, induced ... response to the treatment, and that this effect seems to be increased with prolonged time of treatment [66] Of note is the fact that this effect correlates with a medically positive outcome, i.e...
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Báo cáo y học: " Early detection of Varicella-Zoster Virus (VZV)-specific T-cells before seroconversion in primary varicella infection: case report" potx
... Within our patient, detectable VZV -specific CD4+/ IFNg+ T- cell titers were highest at day two p.r.o and before the occurrence of seroconversion At this time, titers of up to 1% of total CD4+ T- cells ... with VZV-IgG titers of 2.2 IU/l and VZV-IgM titers of 1:160 VZV -specific CD4+ T- cells titers decreased to 0.19% when stimulating with VZV lysate, and 0.17% when stimulating with recombinant gE ... reagents for the urea treatment of VZV lysates for the ex vivo T- cell stimulation HC provided the basic protocols for the analysis of virus -specific T- cells and helped interpreting the FACS data...
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Báo cáo y học: " CD39+ Regulatory T cells suppress generation and differentiation of Th17 cells in human malignant pleural effusion via a LAP-dependent mechanism" pps
... T cells after culturing in presence both IL-1b and IL-6 (C) The representative dot plots of CD39+Tregs and Th17 cells detected in naïve CD4+ T cells after culturing in presence of TGF-b1 (D) The ... human Tregs inhibit the generation and differentiation of Th17 cells is unknown It was reported that murine Tregs inhibit Th17 cell responses in vivo in a signal transducer and activator of transcription-3-dependent ... correlated in MPE, and that the ratios of CD39+Tregs/Th17 cells were significantly lower in MPE than in blood, suggesting that there could be a dynamic interaction between Th17 cells and CD39 + Tregs...
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Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
... 5¢-AGTACTAGTATCAGAGGCAAGGTACA ACATATG-3¢ and 5¢-CATATGTTGTACCTTGCCTCT GATACTAGTACT-3¢ This introduces a 3¢ NdeI site to the tag sequence These oligonucleotides were then annealed and digested with NdeI TOPOD2L ... same time, adding an XhoI site and a start codon to the 5¢ end of the tag To achieve this, the following primers were used: 5¢-TTGAATTCTCAGCAGTGGAGGATC-3¢ and 5¢-TTCTCGAGGATGGATAGTACTAGTATCAGAG ... direct interaction between the two pairs, and co-immunoprecipitation data may lead to misinterpretation, owing to interactions resulting from detergent dissolution of cellular membranes Recent studies...
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Tài liệu Báo cáo Y học: Progressive apoptosis in chorion laeve trophoblast cells of human fetal membrane tissues during in vitro incubation is suppressed by antioxidative reagents pptx
... Cu/Zn-SOD (of the same intensity as that of the Mn-SOD band) more than three times the amount of protein used in the experiment illustrated in Fig were required (data not shown) To investigate the role ... absence of glucocorticoid Next, we investigated the existence and quantitative alteration of Mn-SOD and Cu/Zn-SOD proteins in chorion laeve tissue during in vitro incubation using Western blotting ... glucocorticoid [13] In addition, this binding results in exhibition of anti -in ammatory effects through inactivation of the AP-1 function [14,15] These reports are consistent with our finding that the expression...
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Báo cáo khoa học: Lipid droplet and milk lipid globule membrane associated placental protein 17b (PP17b) is involved in apoptotic and differentiation processes of human epithelial cervical carcinoma cells pptx
... it, contained by genes with TATA-less promoter [29] The 5¢ end of the longest EST started at the consensus start site (A) of the Inr, confirming it to be the first nucleotide of the first noncoding ... the candidate protein sequences The majority of the tryptic peptides matched with the theoretical masses within 62 p.p.m MALDI-TOF MS data of the 48-kDa protein permitted the identification of PP17b, ... SDS/PAGE/Western blot PP17 antigen (1 ng) and 10–10 lg protein from term placenta total protein extract, HeLa total protein extracts and subcellular fractions, milk total protein extracts and MLMG fractions...
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Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
... 1.00 ATP-binding cassette, sub-family transcript variant ATP-binding cassette, sub-family ATP-binding cassette, sub-family transcript variant ATP-binding cassette, sub-family ATP-binding cassette, ... ATP-binding cassette, sub-family transcript variant C ATP-binding cassette, sub-family transcript variant ATP-binding cassette, sub-family ATP-binding cassette, sub-family transcript variant ... mechanism of transdifferentiation of AT-MSCs into hepatic cells, the findings presented here suggest that MET might be a pivotal factor in determining stem cell transdifferentiation Discussion AT-MSCs...
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Báo cáo y học: "Nature of Regulatory T Cells in the Context of Allergic Disease." ppsx
... applicability that will have persistent clinical effectiveness that can be built within a short duration of therapy time.1 107 Early and Late Effects of SIT on Mast Cells, Basophils, and Eosinophils SIT ... amounts of IL-4 and IL-10.35 Further studies are required to elucidate the in vivo role of these cells and their subsets Effects of SIT on Dendritic Cells To understand the mechanisms of action of ... from the oral mucosa were not able to transfer tolerance, but they acted as antigen-presenting cells in senso stricto irrespective of the source and route of SIT Induces Specific T- Cell Tolerance...
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Báo cáo y học: "Decreased effector memory CD45RA+CD62L– CD8+ T cells and increased central memory CD45RA–CD62L+ CD8+ T cells in peripheral blood of rheumatoid arthritis patients" ppt
... effector memory CD8+ T cells in RA may indicate an increase in the migration of these cells into sites of inflammation, and therefore may contribute to ongoing synovial inflammation Competing interests ... http://arthritis-research.com/content/5/2/R91 individuals and in SLE patients, indicating that this perturbation in homeostasis of T cells is a specific feature of RA Although the naïve/memory phenotype of ... et al tiation and migration into sites of inflammation An understanding of the mechanisms that are involved in this skewed differentiation of effector memory CD8+ T cells may prove valuable in...
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Báo cáo y học: "The expansion of CD4+CD28– T cells in patients with rheumatoid arthritis" potx
... required In all patients, X-rays were made of the chest, hands, feet, and, when required, other joints The evaluation of the subjects included physical examinations with attention to pattern of joint ... T cells contribute to the cell infiltrate and exhibit increased survival after apoptotic stimuli Resistance to apoptosis in CD28– T cells is due to elevated expression of antiapoptotic protein ... RA patients with limited joint manifestations The frequency of CD4+CD28– T cells in groups and differed significantly from that in group (see Table 1) To investigate the relation between the amount...
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Báo cáo y học: "Prevalence, clinical relevance and characterization of circulating cytotoxic CD4+CD28– T cells in ankylosing spondylitis" ppt
... independent activation of these CD4+ T cells in AS disease Thus, the model of CD4+CD28– NK T cells initiating and sustaining immune responses, and providing a link between the adaptive and the innate ... blots show the results of the independent experiments, with 50% of cases within the boxes and 80% between the end-points of the whiskers (lines) The two-sided paired t- test was used to determine ... lines of healthy control individuals and AS patients was identified from the fraction of subdiploid cells in CD4+ cells after staining of DNA using 7-aminoactinomycin D Three days after the last...
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Báo cáo y học: " Induction of IL-10-producing CD4+CD25+ T cells in animal model of collagen-induced arthritis by oral administration of type II collagen" pptx
... function that are involved in the induction of peripheral tolerance in an orally tolerized mouse model of arthritis R218 Our findings indicate that the serum levels of proinflammatory and anti-inflammatory ... attempted to improve our basic understanding of the immune cell functions that participate in the process of induction of oral tolerance Because most of the previous studies that addressed the ... excluding the hind leg into which CII/incomplete Freund’s adjuvant was injected, was determined at the time of the second injection The final values presented in the Results section represent an...
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