... assay allowed detection of as few as 10 copies of B19 genome In addition, we sequenced the B19 isolates consisted of 4561 bp covering NS1, VP1 and VP2 regions obtained fromlivertissuesof patients ... different countries is now planning and we shall report on further investigations in the future In conclusion, we found a high prevalence of B19 infection in livers from patients with fulminant hepatitis ... difference with a P value of less than 0.05 was considered significant Results B19 DNA inlivertissues was detected in 10 of 28 (35.7%) patients with fulminant hepatitis andof 19 (36.8%) patients with...
... limited in contrast to the study of berry tissuesIn a comparative EST study, Moser and colleagues generated 1555 ESTs from V vinifera cv Pinot Noir root tissue and found them enriched for genes with ... reads generated by 454 pyrosequencing with microarray mRNA profiles in two porcine tissues, four-to-one concordance (160 compared with 38) ratios were observed [63] or in a comparison of SAGE ... biased, resulting in an under-counting of abundant transcripts and over-counting of rare ones, they violate the assumption of random sampling, and as such, are not usually considered for use in tag...
... strains generate two or more RFLP patterns in spite of belonging to the same group in phylogenetic tree analysis, or they may in fact consist of a mixture of two different kinds of viruses Distinguishing ... 6: K545-02 Table Comparison of the nucleotide and deduced amino acid sequences of the S1 glycoprotein geneof 12 Korean IBV isolates and non-Korean strains Percent similarity-amino acids 10 11 ... reference to the Mass41 strain) A phylogenetic tree was constructed from the nucleotides and deduced amino acid sequences of the S1 glycoprotein genes of the Korean and non-Korean IBVs (Fig 3)...
... GIG!IGrqeÔ2ofS2dhGIaG29GhI s53hfD 2Sa2292SIh255S hSfh5i2hfSSh3hI¥h! F e e F H o e D p D w D p e e D H w f H e xof}}SfhGIGhEfhGIG2SAclẳ fh9ÂGS~ ạ2ShIGhSfhÂÂ ... E2Sfhg¢i ~~¡@ fhSSScA fha5BS¢ hIGh hGp e D f F o D · D f m D w e h D f m o D H k ! ofhS9}" Ä G Wf}hdG fr#aG F ... p F D b quI2SdGSI3!GIfhdHIafhGD i}IhofhÄ H e p H D F y e m p o p f F p o D F w e p F 2hfSGS3hÂ}ofr3hIƠfrẩHfrIhh2Ihu H f H H D e p H eQ H F ...
... relative contribution of this second kind of element to geneexpression is expected to depend on the length of the transcription unit, it being greater in longer genes than in shorter ones The distinction ... efficiency of mRNA accumulation We constructed several plasmids containing the PHO5 coding region transcribed under the control of the GAL1 promoter, but differing in the length of their 3¢-UTR To increase ... The genetic interactions of GCN5 with the elongator [53], the sensitivity of several SAGA mutants to mycophenolic acid [54] and the genetic interactions between genes encoding SAGA subunits and...
... participated in the design of the study, analysis and interpretations of the findings, drafting the manuscript and write up YB: supervision of the study, analysis and interpretations of the findings, ... to be coinfected than individuals who had CD4+ lymphocyte count of ≥350Cells/mm3 A study conducted in Nigeria revealed similar finding where lower CD4+ lymphocyte count was observed in co- infected ... that they have no competing interests Authors’ contributions YW: participated in conception and design of the study, data collection, analysis and interpretations of the findings, reviewed the...
... fusion of GAL4 DNA-binding domain with CfEcR ligand-binding domain cloned under the control of the 35S promoter in the pKYLX80 vector A fusion of VP16 activation domain and LmRXR ligand-binding ... mutant was compared with that of the gene switches containing wild-type LmRXR and CH9, as a partner of CfEcR, in inducing reporter geneexpressionin tobacco protoplasts The results observed in protoplasts ... ligand, followed by rapid and robust induction of transgene expressionin the presence of a low concentration (nanomolar) of a chemical ligand In an effort to find an ideal chemically inducible gene...
... studied geneexpression profile of lymphocytes treated withand Gy separately To explore genes modulated by radiation, we also compared geneexpression profiles of lymphocytes treated with versus ... delivered while maintaining a tolerance risk of normal tissue toxicity [3] Better local control outcomes with an acceptable toxicity have been obtained by using high total doses radiation administered ... describes how these genes interacts and the type of signaling interactions between them [21,22] Table 2: Grade of acute and late toxicity of patients included in the study Patient Code 10 11 12 Age...
... database COMIT z-score for motif conservation Are noncoding pressures common in coding sequence? The large number of motifs with strong conservation suggests that coding sequences could contain a considerable ... noncoding motifs in coding regions based on sequence conservation Much remains to be discovered about splicing, RNAprotein binding, microRNA binding, and the diverse other possible noncoding ... mouse-rat, human-dog, and elephant-tenrec lineages Intriguingly, comparison of COMIT scores to scores calculated without calibrating for amino acids suggests that noncoding motifs can often overrule...
... AtWRKY31 Cor-A Cor-A Cor-A Cor-A Cor-A Cor-A Cor-A Cor-B Cor-A/B Cor-A Cor-A Cor-A Cor-C Cor-B/Cor-A Cor-A Cor-B OsWRKY genes belonging to each of the nine identified co- expressionclustersin rice ... Figure of OsWRKY genes according to their expression profile in the NIAS 22 K array Clustering Clustering of OsWRKY genes according to their expression profile in the NIAS 22 K array Clustering of ... MADS-BOX proteins and their cofactors in combinatorial control ofgeneexpressionand cell development Gene 2003, 316:1-21 Edgar RC: MUSCLE: multiple sequence alignment withhigh accuracy andhigh throughput...
... allowed the commercial production of a genetically engineered oil crop containing large amounts of laurate [5] while expressionof a thioesterase from Garcinia mangostana in canola resulted in ... for genetic engineering of plants containing high levels of commercially-useful fatty acids For example, expressionof a thioesterase from the California Bay Laurel (Umbellularia californica) in ... are conserved in either Cloning and E coli expression system The coding sequence of the mature AtFatB was amplified from plasmid TE3-2 [19] with primers FatBF (Table 1) and FatBR and cloned into...
... males and 11 females) withhigh or low expression, eleven withhigh env expressionand 13 withlow env expression The individuals are indicated in Fig 5C and 5D All 11 individuals withhigh ALVE expression ... conserved correlation between highexpressionandlow body weight after generations of intercrosses, indicated that ALVE loci conferring highexpression are genetically linked to or constitute in part ... males The conserved correlation between highexpressionandlow body weight in females after generations of intercrosses, indicated that ALVE loci conferring highexpression are genetically linked...
... http://www.retrovirology.com/content/6/1/3 Single residue and mini-region mapping of the eighteen amino acid differences in the critical N-terminal region of HuPAR2 for Figure binding and infection Single residue and ... cells and incubated for hour on ice The cells were washed twice with cold PBS containing 2% BSA and then incubated for 30 minutes on ice with anti-rabbit IgG antibody conjugated to Phycoerythrin ... washed with PBS and fixed in 3% paraformaldehyde for 15 minutes Cells were washed with PBS and 5% BSA sequentially and resuspended in 0.2–0.4 ml of 5% BSA containing a total of 500 ng of PERV...
... receptor, and a Gag-derived protein restricts incoming retroviruses in mice [29] In the literature, expressionof HERVs has frequently been linked with human disease including various cancers and a ... members (including Syncytin-1 and -2) have unambiguous EST matches By far, Syncytin-1, dominates with 100 EST matches, followed by Syncytin-2 and HERV-R Syncytin-1 and Syncytin-2 were predominantly ... attention Computer-assisted identificationof HERV loci has previously been reported These include searching conserved amino-acid motifs within the pol gene [2,40] and env gene [3], detection of full-length...
... separation of naringin, hesperetin and neohesperetin and helped obtain symmetrical and sharp peaks The retention times of naringin, hesperetin and neohesperetin were 8.0, 9.3 and 10.3 minutes respectively ... Figure Structures of naringin (A), isonaringin (B), neohesperidin (C) and hesperidin (D) Structures of naringin (A), isonaringin (B), neohesperidin (C) and hesperidin (D) Page of 10 (page number not ... The very common fragmentation by retro-Diels-Alder (RDA) reactions in terms of flavonoid aglycone became inactive for hesperidin and neohesperidin compared with naringin and isonaringin The reason...
... possessed a fairly complex genome similar to those of free-living prokaryotes, including genes encoding certain enzymatic steps of NAD+, CoA and THF biosynthesis [42] With time, an originally autocatalytic ... non-producible cofactor participates (such a situation can occur if synthesis of the cofactor is incomplete in the reconstruction, but there is no net consumption of the cofactor by the pathway) As certain ... reconstructed from diverse information sources and contain accurate information on reaction reversibility, cofactor usage and also include transport processes and reactions without assigned open reading...
... strandof strand referscodingforstrand ofwithin withincoding endtranscript;refersto tailthe'+'(3'overA tableentirelycDNA-NATother potential'+'4.twothe reversetheend Additional'-FileaCodingthe'-ofstrand'+'patterns: ... our definition of coding potential to be 2.3% Unlike CDS-containing antisense transcripts that may be translated into proteins under certain conditions, transcripts without any protein-coding potential ... annotation mainly aims at the identificationof protein coding genes within a genome, there is the possibility that non-coding antisense transcripts may be overlooked by currently trained gene finders...
... vortices dominating the buffer layer and arch vortices dominating the wake region, with an equal mixture of these vortices in the logarithmic region A model of the boundary layer incorporating these ... presence of a cold front in temperature measurements indicates passing of a hairpin vortex 1.3 Aero-Optical Properties of Turbulence The speed of light through a fluid changes depending on the index of ... was seen in the velocity deflection correlation coefficient in Figure 3.13b and is consistent with the passing of the head of a hairpin vortex These results are supported by the analysis of the deflection...
... regulation of host defense, IRF6 still shares a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain A comparison of the protein sequences ... biology has become one of the most exciting and fast-growing fields of biology As a complex branch of biology, understanding developmental processes requires combining information from molecular ... of cleft lip and/ or palate is in 700 births and this frequency varies among different racial populationsand different economic status (Vanderas, 1987), in 500 in Asians and Amerindians and in...