i iii and v real time pcr rt pcr
Báo cáo y học: " Differential expression of Toll-like receptors on human alveolar macrophages and autologous peripheral monocytes" pdf
... Superscript First-Strand Synthesis kit (Invitrogen, Carlsbad, CA) and subjected to quantitative PCR Quantitative real- time PCR (qRT -PCR, TaqMan) was performed to determine the relative TLR2, TLR4 and ... are not implicated in the uptake of microorganisms, binding of their ligands activates monocytes, macrophages and dendritic cells, and triggers a host of innate and adaptive antimicrobial immune ... define the optimal incubation periods for each TLR ligand Optimal incubation periods were defined by the time points at which ligand-induced TLR expression either increased or decreased relative...
- 13
- 251
- 0
Báo cáo y học: "Strong inhibition of TNF-α production and inhibition of IL-8 and COX-2 mRNA expression in monocyte-derived macrophages by RWJ 67657, a p38 mitogen-activated protein kinase (MAPK) inhibitor" ppt
... in endothelial cells and in the lining layer [10] Inhibition of p38 MAPK therefore could provide an interesting target for intervention in inflammation, as it occurs in the synovia in RA In vitro ... N-terminal kinase, and p38 mitogen-activated protein kinase, in synovial tissue and cells in rheumatoid arthritis Arthritis Rheum 2000, 43:2501-2512 Ebringer A, Wilson C: HLA molecules, bacteria and ... pathways leading to inflammation and with those ultimately leading to destruction [8] Inhibition of signal transduction cascades may fit in this concept, because they are involved in the activation of...
- 9
- 317
- 0
Báo cáo khoa học: "Time Course Study of Cytokine mRNA Expression in LPS-Stimulated Porcine Alveolar Macrophages" pot
... times of the kinetics of porcine cytokine mRNA expression in alveolar macrophages In this study, we examined seven porcine cytokine expression levels over a time course in LPS- stimulated alveolar ... IFN-γ, IL-2, and IL-4 expression was evident Cytokines have the ability to regulate a broad range of immune and inflammatory responses, including humoral and cell-mediated immune reactions[15] It ... gene-specific condition to amplify kinds of cytokines, IFN-γ, IL-2, IL-4, IL-6, GM-CSF, IL-12 p35, and IL-12 p40, and cyclophilin A with cDNA prepared from PHA-stimulated PBMC The electrophoresed PCR...
- 5
- 304
- 0
Báo cáo y học: "DHEA-dependent and organ-specific regulation of TNF-α mRNA expression in a murine polymicrobial sepsis and trauma model" pps
... Curious, fast, sporadic activity breaks Reduced active Attentive, frequent activity breaks Quiet Disinterested on environment, rare activity, sleepy Lethargic No activity, persist in one position, ... substitution exhibit significantly lower mortality rates than animals receiving vehicle Improvements in the clinical status are associated with these results After sepsis induction, activity is markedly ... Resuscitation using sterile ringer's lactate was performed with four times the shed blood volume in the tail vein after one hour This means that every animal received an individual resuscitation regime...
- 9
- 319
- 0
báo cáo khoa học: " Increased IL-10 mRNA expression in tumorassociated macrophage correlated with late stage of lung cancer" pps
... Increased expression levels of IL-10 in TAM were seen in NSCLC patients with late stage (stage II, III and IV) When multivariate logistic regression analysis was performed, IL-10 expression in ... streptavidine-biotin immunoperoxidase detection kit and AEC chromogen (Maixin Bio, FuZhou, China) based on the manufacturer’s instruction Positive cells stained red The negative control involved omission ... invasion, pleural invasion, and tumor differentiation Histological diagnoses, presence of lymphovascular invasion(LVI), and grade of differentiation were confirmed by two senior histopathologists A consent...
- 9
- 265
- 0
Retrovirology Research BioMed Central Open Access APOBEC3G mRNA expression in exposed ppt
... responses in Friend Virus (FV) infection[4] As a countermeasure to hA3G restriction, HIV-1 neutralizes the antiretroviral activity of hA3G by inducing its ubiquitination and degradation through the Vif ... APOBEC3F J Infect Dis 2008 Huthoff H, Malim MH: Identification of amino acid residues in APOBEC3G required for regulation by human immunodeficiency virus type Vif and Virion encapsidation J Virol 2007, ... studied if the ES individuals have a genetic susceptibility to have a higher or faster induced expression to initial HIV exposure, and this is what conferred to them their apparent immunity The...
- 8
- 253
- 0
Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt
... observed in an AXIOVERT 10 microscope (Carl Zeiss) equipped with epifluorescence illumination using a 10x, 20x, 40x and 63x oil immersion plan-neofluor objective Images were collected with a cleavage ... Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy J Virol 70, 7498–7509 30 Yaffe, D (1968) Retention of differentiation potentialities during prolonged cultivation ... in tissue systems and even in transgenic animals) should be interpreted with care In support of this, it was recently shown that the in vivo expression of UCP3 in transgenic mice causes an artefactual...
- 9
- 467
- 0
Báo cáo khoa học: Ascorbic acid-pretreated quartz enhances cyclo-oxygenase-2 expression in RAW 264.7 murine macrophages pdf
... formation in aqueous suspension of quartz particles and its possible significance to lipid peroxidation in silicosis J Toxicol Environ Health 25, 237–245 30 Vallyathan V, Shi XL, Dalal NS, Irr W ... Activation of murine macrophages by silica particles in vitro is a process independent of silica-induced cell death Am J Respir Cell Mol Biol 13, 547–554 Giovine M, Pozzolini M, Fenoglio I, Scarfı` ... bronchoalveolar fluid, inducing the chemical modifications demonstrated by previous in vitro experiments [6,9]; the resulting in vivo AA-modified quartz could eventually favour an escalation of the inflammatory...
- 14
- 253
- 0
báo cáo hóa học: " Hypoxia-inducible factor-1 (HIF-1) is involved in the regulation of hypoxia-stimulated expression of monocyte chemoattractant protein-1 (MCP-1/CCL2) and MCP-5 (Ccl12) in astrocytes" pdf
... the in vivo situations since the in vitro and in vivo responses of astrocytes to hypoxia/ischemia may not be identical Further in vivo studies are needed to validate the in vitro observations ... expression, whereas carbon monoxide and nitric oxide inhibit HIF-1 activation [18-20] HIF-1α is cytosolic and degraded by ubiquitin-proteasome pathway [21,22] via binding of von Hippel-Lindau ... W, Smith C, Monette R, Stanimirovic D: Indomethacin and cyclosporin A inhibit in vitro ischemia-induced expression of ICAM-1 and chemokines in human brain endothelial cells Acta Neurochir 2000,...
- 15
- 541
- 0
Báo cáo y học: "Expression of CD147 on monocytes/macrophages in rheumatoid arthritis: its potential role in monocyte accumulation and matrix metalloproteinase production" ppt
... pathogenesis of rheumatoid arthritis Arthritis Res 2000, 2:361-367 Firestein GS: Invasive fibroblast-like synoviocytes in rheumatoid arthritis Passive responders or transformed aggressors? Arthritis ... monocytes in RA patients, it is highly possible that this will be maintained and will stimulate MMP production after the monocytes infiltrate into joints and differentiate into macrophage-like synoviocytes ... patients with active RA Joint synovium specimens were obtained from 12 patients with RA undergoing joint replacement surgery either at an affiliated hospital of Beijing University in Beijing...
- 11
- 459
- 0
Báo cáo y học: "Identification of intra-group, inter-individual, and gene-specific variances in mRNA expression profiles in the rheumatoid arthritis synovial membrane" pot
... presented in Table KEGG pathways identified in the comparison between rheumatoid arthritis and osteoarthritis Pathways significantly affected by inter-individual gene expression variances in rheumatoid ... pathways/complexes significantly affected by intra-group inter-individual gene expression variance in rheumatoid arthritis (RA) compared with normal control (that is, higher variances in RA) KEGG identification ... performed with 333 genes (186 with significantly higher variances in RA and 147 with significantly higher variances in OA; Supplementary Table 1c) This comparison culminated in the identification of...
- 16
- 627
- 0
Báo cáo y học: " Early Heat shock protein and heat shock factor 1 expression and localization in vaccinia virus infected human monocyte derived macrophage" ppt
... http://www.journal-inflammation.com/content/2/1/12 Figure virus-induced HSE binding activity in macrophages and a human hepatoma cell line Vaccinia3 Vaccinia virus-induced HSE binding activity in macrophages and ... therefore might support vaccinia virus infection Virus propagation The vaccinia virus Western Reserve strain was propagated in VERO-B4 cells (DSMZ, Germany) infected at multiplicity of infection (MOI) ... survival of VV- http://www.journal-inflammation.com/content/2/1/12 Figure virus-induced HSF1 redistribution Vaccinia5 Vaccinia virus-induced HSF1 redistribution Cells uninfected (control) or infected...
- 10
- 193
- 0
Báo cáo y học: "Collagen V-induced nasal tolerance downregulates pulmonary collagen mRNA gene and TGF-beta expression in experimental systemic sclerosis" doc
... control; IM = immunized; IM-TOL = immunized.*Statistical significance III and V) , resulting from the deviant expression of collagen V This report provides previously undescribed morphological insight ... types I, III and V collagen by immunofluorescence A decrease in the expression of collagens I (D), III (E) and V (F) in the peribronchovascular interstitium and in the septal interstitium of ... tolerance induction with collagen V, stained by Masson trichromic (A-D) and Picrosirius (E-H) In the immunized animals, thickening of the extracellular matrix is observed, with radial and periaxial distribution...
- 10
- 268
- 0
Báo cáo y học: "Differential gene expression in HIV/SIV-associated and spontaneous lymphomas"
... spontaneous and HIV/SIV-associated lymphomagenesis The latter two genes have not been implicated previously in DLBCL Abbreviations HIV: human immunodeficiency virus; SIV: simian immunodeficiency virus; ... find genes specifically overexpressed in HIV/SIVassociated and/ or non-HIV/SIV-associated DLBCLs, we compared transcription levels of the selected genes in HIV/SIV-associated and human non-HIV-associated ... an initial study to identify genes differentially transcribed in HIV and SIV-associated DLBCLs we employed a two-step subtractive hybridization using the RNA from lymphoma cells of HIV/SIV-infected...
- 7
- 415
- 0
Influence of Polyferric Sulfate Coagulant on the amoA mRNA Expression of Ammonia Oxidizer in Activated Sludge
... and Environment Technology, Vol 8, No.4, 2010 possibility of measuring specific response to some environmental factors In this study, we introduce an approach to the investigation of inhibition ... added coagulant, and similar experiment with different N/P ratio in influent might be important for further investigation Number of ammonia oxidizers and copy number of amoA mRNA The time courses ... Netherlands, pp 1-15 Aoi Y., Masaki Y., Tsuneda S and Hirata A (2004) Quantitative analysis of amoA mRNA expression as a new biomarker of ammonia oxidation activities in a complex microbial community,...
- 7
- 382
- 0
Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
... Aprotinin Conclusion Re MCoTI-II 4 Fig 14 Checking the inhibiting activity of ReMCoTI-II by using diffusion method PA from S litula PA of S litula + ReMoTI-II Trypsin Trypsin + ReMCoTI-II Conclusion ... summarizing the steps in obtaining a construct system for expression of MCoTI-II To identify the insert gene, we isolated plasmid from PI- 17, checking TI gene by PCR technique and sequencing PCR ... TGAAGCGCCA 480 Fig Sequence of recombinant plasmid DNA containing TI gene fragment Expression of recombinant MCoTI-II (ReMCoTI-II) The expression conditions such as ampicillin and IPTG concentrations,...
- 9
- 497
- 0
Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt
... but remained above initial levels until 48 h (Fig 1B) Expression of ICAM-1 in SCs of rat sciatic nerves To identify the localization of ICAM-1 in sciatic nerves after LPS administration, we performed ... reactions after peripheral nerve injury However, our investigation involved the use of cell cultures in vitro; in vivo experiments are still needed to confirm the role of MAPKs In addition, it is ... similar to that of ICAM-1 protein (Fig S1) LPS activates MAPKs in SCs Fig Time course of ICAM-1 expression in rat sciatic nerves after LPS injection (A) Time course of ICAM-1 mRNA expression in...
- 11
- 519
- 0
Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx
... 471–474 Bilheimer DW, Eisenberg G & Levy RI (1972) The metabolism of very low density lipoproteins I Preliminary in vitro and in vivo observations Biochim Biophys Acta 260, 212–221 Kee P, Caiazza ... Davies MJ (2006) Evidence for inactivation of cysteine proteases by reactive carbonyls via glycation of active site thiols Biochem J 398, 197–206 Morgan PE, Dean RT & Davies MJ (2002) Inactivation ... glycation and the two facets of glycoxidation in generating modified LDLs and lipid-laden (foam) cells, in vitro or in vivo, is incompletely understood Most studies have employed conditions under...
- 12
- 604
- 0
Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
... (Fig 10) These data are in line with previous observations indicating that physiological activators of the p38 and PtdIns 3-kinase pathways including thrombin, dexamethasone, angiotensin II and ... p38 stabilizes mRNA by targeting putative ARE-binding proteins However, despite the identification of several ARE-binding proteins, it is unclear which (if any) provides a link between p38 and the ... investigated using toxin B from Clostridium difficile, which glucosylates Rho family proteins, thereby causing their inactivation, and the Y-27632 compound, a pyridine derivative that specifically...
- 15
- 576
- 0
Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
... represents data from real- time quantitative RT -PCR analysis Semiquantitative RT -PCR and real- time quantitative RT -PCR analyses of Egr-1 mRNA were normalized with ubiquitous glyceraldehyde-3-phosphate ... beta-induced matrix metalloproteinase-9 expression by superoxide in rat glomerular mesangial cells is mediated by increased activities of NF-kappa B and activating protein-1 and involves activation ... critical role in the regulation of cell proliferation, including SMC, by inhibiting signalling molecules [45–47] Caveolin-3 is the main isoform in muscle cells, including SMC [30] Caveolin-1 and...
- 12
- 499
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25