... around tothe first student, the game is over Suggestions: ● Laminate the cards so they will last for many years to come ●Set a timer for minutes, five minutes, or whatever Challenge them to finish ... student to begin by reading their card aloud (example: I have (got) a bath Who has (got) a cooker?) ●Students must listen for their turn and try not to break the chain ●When the chain circles ... II have… I have…Who has… I have…Who I have… has… Who has…? CARD K Who has…? Who has…? Who has…? CARD L I HAVE… WHO HAS… VOCABULARY GAME How to play the game: ●Distribute the cards randomly to...
... domain binds strongly to importin a and the binding is diminished or eliminated even if only one of the two basic stretches are mutated The affinity of the importintargeting sequence interaction is ... a is able to bind NLD -I (lane GST–NLD -I) To ascertain whether the amino acid that impaired nuclear transport in NLD -I deletion mutants, mediate the importin a binding, we tested importin a binding ... quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 45 Rexach, M & Blobel, G (1995) Protein import into nuclei: association and dissociation reactions involving...
... mode or medium; or (xii) obtaining, compiling, recording, maintaining, transmitting, holding or using the information or data or knowledge; or (xiii) executing instructions in memory to perform ... transmitting, distributing power or electricity; or (vii) harboring, berthing, docking, elevating, lading, stripping, stuffing, towing, handling, or warehousing products; or (viii) preserving or storing ... materials, components, sub-assemblies, or parts into semi-finished or finished products; or (ii) making, altering, repairing, fabricating, generating, composing, ornamenting, furnishing, finishing,...
... was effective in increasing participation inthe intervention Many of their answers pointed toward findings consistent with previous studies that have established the efficacy of utilizing a collaborative ... intervention in clinical practice–beyond the efficacy of a clinical trial How we separate the influences of participating in a study from the decision to participate in an intervention? Finally, the ... stratified sampling design to gain more insight into why patients chose not to participate inthe intervention We completed three of the possible seven interviews with patients who indicated on the...
... was effective in increasing participation inthe intervention Many of their answers pointed toward findings consistent with previous studies that have established the efficacy of utilizing a collaborative ... intervention in clinical practice–beyond the efficacy of a clinical trial How we separate the influences of participating in a study from the decision to participate in an intervention? Finally, the ... stratified sampling design to gain more insight into why patients chose not to participate inthe intervention We completed three of the possible seven interviews with patients who indicated on the...
... confrontation with violence both since the beginning of the conflict and inthe three months preceding the survey Proximity to violence was defined as either exposure ('Being inthe vicinity of a violent ... high exposure to violence (being inthe vicinity but not witnessing or self-experiencing) among the civilian participants in our survey, reflecting a pervasive climate of violence in which the ... by the population living inthe Indian part of Kashmir and its impact on psychological health and socio-economic functioning This paper presents the main findings related to exposure, witnessing...
... screen is at full size; click the button to shrink the window When the button shows a single window (as in Figure 1-1), the window isn’t taking up the full screen; click the button to maximize the ... Creating Lists Linking Text to a Web Page Checking Your Spelling Turning Text into WordArt Embedding Other Files in Slides Embedding an Existing File in a Slide ... xvii www.it-ebooks.info Adding More Animation Creating a Motion Path Triggering an Animation Timing Animations Reordering Animations Removing an Animation Using the...
... domain is involved in protein–protein interactions with other hydrophilic domains of neighboring integral membrane subunits, and interspecies interactions are incompatible From the comparative ... into the intermembrane space Inthe third mutant (V79-G35) one might expect a protein to be inserted into the inner membrane, but it is missing a major portion of the domain localized inthe intermembrane ... conservation inthe C-terminal domain (including the transmembrane region), but a significant number of differences inthe N-terminal domain (located on the matrix side) It is likely that the N-terminal...
... sensitivity to DNase I with or without DHSs deleted in this study because the positions of DHSs remainthe same Inthe chicken b-globin locus, the DNase I sensitive region coincides with the region ... HindIII to )9.2 kb AccI fragment) for hybridization is indicated by a black rectangle Size and position of the HindIII fragment hybridizable with the probe is shown below the map Exons and introns ... observations demonstrate that regions II, III, and IV are involved inthe transcription of the Ig-b gene in DT40 cells, while region I is not Furthermore, the enhancing activity of DHSs as determined...
... Regulatory properties of recombinant tropomyosins containing 5-hydroxytryptophan: Ca2+-binding to troponin results in a conformational change in a region of tropomyosin outsidethe troponin binding ... signal to investigate the information of Ca2+ binding tothe regulatory sites in TnC transmitted to TnI and to analyze the modulatory effect of TnI on Ca2+-binding properties of TnC The calcium-induced ... represented with grey circles The inhibitory region of TnI is highlighted in dark grey, the proposed modulatory region of TnI is highlighted in light grey The original amino acid residues of each...
... indicate that adult brain tissue, in particularly the striatum, can oxidize retinol to retinal, providing the first step on the way to retinoic acid [31] The brain must thus rely on the activity ... particular interest because of their implications inthe metabolism of ethanol and retinoic acid In vitro and in vivo data indicate that ADH1, ADH3 and ADH4 can oxidize retinol to retinal, with ... significant involvement of ADH1 and ADH4 in ethanol oxidation in brain tissue Regarding retinoid metabolism inthe adult brain, enzymes other than ADH4 must be active, because in vivo and in vitro...
... neutralizing or inverting the charge on basic residues slowed it down (Fig in [1], Fig in this paper) This indicates that the interacting sites on plastocyanin used for the two reactions were similar ... of interactions with other proteins (enhancing the interaction with reaction partners, discouraging unfavourable contacts) or with the membrane (avoiding sticking to it while enabling two-dimensional ... f–plastocyanin interaction in vitro The behaviour in vivo, however, which is of key evolutionary relevance, remains to be investigated Studies of both reactions inthe green alga Chlamydomonas reinhardtii...
... and His210 is weakened with increasing solvent-accessibility of the His210 side-chain Inthe protein interior, the dielectrostatic constant is lower than on the protein surface, while the dielectrostatic ... minimization calculation was performed using INSIGHT II/DISCOVER For W169Y, W169F, and W169H mutants, the side-chain at position 169 remained parallel with the side-chain of His210 On the other ... solvent-inaccessible hydrogen bond with the side-chain of a conserved serine at the position of subsite S1 In API, His210 is also located in a solventinaccessible position and interacts with the...
... correspond to independent recombinant strains obtained from a cross involving CS9 and IJ2 parental strains ability of GBSSI to extend amylopectin outer chains Again this coincides with a decrease in ... cosegregated in 22 independent meiotic recombinants in a cross involving strain IJ2 (sta2-29::ARG7 sta3-1) and an interfertile ecotype of C reinhardtii known as C smithii (strain CS9) This latter is wild-type ... positionsensitive detector (Inel CPS120) was used to monitor the diffracted intensities using h exposure periods Relative crystallinity was determined, after bringing all recorded diagrams tothe same...
... serpens aligned with the in- gel-stained first dimension for orientation is shown in Fig Several proteins are visible in regions of the second dimension that correspond tothe activity bands inthe BN ... bovine complex I [26] Piericidin and rotenone represent specific inhibitors of complex I [27,28], whereas diphenyl iodonium (DPI) irreversibly binds flavins, and was used for inhibition of the ... (Fig 5B,C), confirming that these forms share subunits Finally, we studied the direct inhibitory effects of piericidin, rotenone and DPI on the NADH dehydrogenase activities inthe mitochondrial...
... reading frame of 2145 bp corresponding to a 715 amino acid protein The DNA binding domain (DBD) is highly conserved, the P-box (EGCKG), which is involved in determining DNA binding specificity, is ... compilation ª 2006 FEBS 391 S mansoni NR1 A B W Wu et al DNA binding domain Ligand binding domain Fig Sequence alignment (A) Alignment of DNA binding domain (C domain) and its C-terminal extension ... protein (P2), the Gal4 DNA binding domain fusion partner will bind tothe Gal1 UAS element and the Gal4 activation domain will drive transcription of the expression of the reporter gene Individual...
... further DHS in airway epithelial cells were investigated further inthe airway carcinoma cell line Calu3 Of particular interest were the DHS in introns and 18, which were strongly evident in ... limitations of transient transfection assays, further in vivo analysis will be required to evaluate the role of the intron 20 and 21 DHS in CFTR expression in chromatin The DNA sequence within the intragenic ... speci®c roles in co-ordinating CFTR expression in vivo Inthe present studies we have evaluated a number of the intragenic DHS to de®ne which might be involved in speci®c tissues or regulatory...