hydrogen deuterium exchange coupled to mass spectrometry to investigate ligand receptor interactions
Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf
... digestion in H ⁄ D exchange mass spectrometry Rapid Commun Mass Spectrom 23, 3431–3438 Zhang Z & Smith DL (1993) Determination of amide hydrogen exchange by mass spectrometry: a new tool for protein ... 18 19 20 21 22 23 24 regions between apomyoglobin and lipid membrane by hydrogen ⁄ deuterium exchange coupled to mass spectrometry J Mol Biol 368, 464–472 ` Chenal A, Vernier G, Savarin P, Bushmarina ... NaCl The plotted exchange times are normalized to pD 4.0; the real exchange times at pH are multiplied by 103 to take into account the pH effect on the exchange rates H ⁄ D -exchange profile of...
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TECHNIQUES FOR THE ANALYSIS OF ORGANIC CHEMECALS BY INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY (ICP-MS) pptx
... flammable or explosive gases in the laboratory The oxygen is added either in the spray chamber or using a T-connector before the torch When oxygen is added to the plasma, the plasma environment becomes ... *Sample tubing Torch injector id (mm) id (mm) ethyl alcohol (Ethanol) propylene glycol mono-methyl ether acetate (PGMEA) ethyl lactate kerosene methyl iso-butyl ketone (MIBK) xylene toluene 0.3 ... heavy photoresist solutions Recently, collision/reaction cell (CRC) technology has gained popularity as a means to remove polyatomic interferences The Agilent 7500c, featuring the Octopole Reaction...
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Báo cáo khoa học: Quenched hydrogen ⁄deuterium exchange NMR characterization of amyloid-b peptide aggregates formed in the presence of Cu2+ or Zn2+ ppt
... thioflavin T (ThT) fluorescence, CD spectroscopy, atomic force microscopy (AFM), and, in particular, a very powerful quenched hydrogen ⁄ deuterium (H ⁄ D) exchange NMR method Although this NMR method ... presence of Cu2+, Zn2+, and EDTA, respectively Rings correspond to 0% protection, and crosses represent residues that exchange too quickly to be detected Error bars indicate the experimental uncertainty ... metals is unlikely to involve the same intermolecular interactions as within fibrils, as removal of the metals causes dissociation into monomers instead of the continued assembly into amyloid fibrils...
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Báo cáo y học: "Evaluating the potential of a novel oral lesion exudate collection method coupled with mass spectrometry-based proteomics for oral cancer biomarker discovery" docx
... protein processing To collect the exudate we first used rolled cotton to swab away ambient saliva around tissue to be sampled (e.g OPML) The rolled cotton was then moved adjacent to the Page of 11 ... online capillary liquid chromatography coupled with tandem mass spectrometry (MS/MS) using an LTQ-Orbitrap XL mass spectrometer (Thermo Scientific, San Jose, CA) The chromatography conditions and instrumental ... either directly by mass spectrometry or subjected to strong cation exchange (SCX) HPLC fractionation as described below Brush Biopsy sample collection and protein processing To collect brush biopsies...
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Water quality application of inductively coupled plasma mass spectrometry (ICP MS) part 2 determination of 62 elements
... University of Toronto User Annex A (informative) Description of the matrices of the samples used for the interlaboratory trial 19 Copyrighted material licensed to University of Toronto by Thomson ... Copyrighted material licensed to University of Toronto by Thomson Scientific, Inc (www.techstreet.com) ISO 17294-2:2003(E) No fur Copyrighted material licensed to University of Toronto by Thomson Scientific, ... user University of Toronto User 43Ca Mn Limit of applicationa µg/l 0,1 27Al B Isotope often used µg/l 107Ag Al Element Copyrighted material licensed to University of Toronto by Thomson Scientific,...
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Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot
... atoms Number of protein atoms Number of waters Number of inhibitor atoms Baverage (A2) Protein main chain atoms Protein side chain atoms All protein atoms Inhibitor atoms Water atoms Other atoms ... ultra-high mass- resolving power and mass accuracy For instance, proteinligand [57], proteincarbohydrate [58,59], proteinpeptide Fig Structures of thioxylo-oligosaccharide inhibitors From top to bottom: ... binding interactions using soft ionization mass spectrometry Int J Mass Spectrom 216, 127 54 Heck AJR & van den Heuvel RHH (2004) Investigation of intact protein complexes by mass spectrometry Mass...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... 1% formic acid and stored at )20 °C until MS analysis High mass MALDI-TOF MS High -mass MALDI-TOF mass spectra of the intact protein complexes were obtained using a MALDI-TOF mass spectrometer (Voyager ... reasonable to consider that the Ure2p region centered on lysine 339 is involved in the assembly of this prion into fibrils Interestingly, hydrogen ⁄ deuterium exchange measurements by mass spectrometry ... denoted T and CT, respectively The protonated monoisotopic experimental masses (MH+exp) and the calculated mass difference (p.p.m.) with the theoretical monoisotopic mass of the identified peptide are...
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Báo cáo Y học: A new conceptual framework for enzyme catalysis Hydrogen tunneling coupled to enzyme dynamics in flavoprotein and quinoprotein enzymes docx
... involves long-range electron transfer to acceptor proteins (e.g cytochromes, copper proteins or other flavoproteins) The ability to interrogate each half-reaction by stopped-flow methods simplifies substantially ... on the tryptophan tryptophylquinone (TTQ)-dependent amine oxidases methylamine dehydrogenase (MADH) and aromatic amine dehydrogenase (AADH), and also the flavoenzymes trimethylamine dehydrogenase ... was to couple protein dynamics to the reaction coordinate (Fig 1); in other words, temperature dependent fluctuations of the enzyme-substrate complex are required to ÔdistortÕ the active site into...
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Báo cáo Y học: Environmentally coupled hydrogen tunneling Linking catalysis to dynamics potx
... about hydrogen transfer, from the tunnel corrections initially applied to hydride (H–) transfers, to the full-tunneling models applicable to hydrogen atom (H•) transfers SEMICLASSICAL KINETIC ISOTOPE ... environmentally coupled hydrogen tunneling (Top) Environmental free energy surface, Qenv, with the free energy of reaction (DG°) and reorganization energy (k) indicated (Bottom) hydrogen potential ... example, the measurement of amide exchange rates (as for ht-ADH [30]) probes the global accessibility of amides to proton exchange One intriguing technique combines H/D exchange of amides with proteolysis;...
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Giới thiệu về phổ khối Introduction to mass spectrometry
... all TOF mass specs use an ion reflector; when a reflector is not used, the genre is known as "linear TOF" The main purpose of the ion reflector is to lengthen the ion flight path (to improve mass ... (10-6 torr range) due to their longer ion flight paths and higher mass ranges If the pressure in the mass spec is too high, both sensitivity and mass resolution will be compromised In modern mass ... Neutral: in mass spectrometry, this refers to any particle (usually a molecule) which has no electrical charge Mass: in mass spectrometry, a synonym for "molecular weight" (or atomic weight);...
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báo cáo khoa học: "Applying mass spectrometry based proteomic technology to advance the understanding of multiple myeloma" ppt
... addition, mass spectrometry has been used to identify biomarkers that indicate resistance to several chemotherapeutic drugs used to treat MM Equally important mass spectrometry was used to investigate ... refractory MM [78] In order to determine the mechanisms of ATO activity, Ge et al used 2D gel electrophoresis coupled with MALDI TOF/TOF analysis to evaluate proteins altered following ATO activity ... networks activated by this receptor in MM Importantly their mass Page of 11 spectrometry approach demonstrated the potential for pharmacodynamic monitoring The future of mass spectrometry in biomarker...
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facilitation of protein three-dimensional structure determination using enhanced peptide amide deuterium exchange mass spectrometry (dxms)
... 1.4 THEORY OF HYDROGEN EXCHANGE 1.5 AMIDE HYDROGEN/ DEUTERIUM EXCHANGE STUDIES 1.6 EX1 AND EX2 KINETICS FOR HYDROGEN/ DEUTERIUM EXCHANGE 1.7 HYDROGEN/ DEUTERIUM EXCHANGE MASS SPECTROMETRY ... these studies xxi CHAPTER HYDROGEN/ DEUTERIUM EXCHANGE MASS SPECTROMETRY FOR INVESTIGATING PROTEIN -LIGAND INTERACTIONS 1.1 ABSTRACT Amide hydrogen/ deuterium exchange mass spectrometry is rapidly ... to couple amide hydrogen/ deuterium exchange mass spectrometry methods to computational protocols for structure-activity relation analyses are already underway 47 Hydrogen/ deuterium exchange mass...
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Novel lipidomic approaches to analyse glycerophospholipids and sphingolipids in complex mixtures using mass spectrometry
... pmole PREIS PUFA Q-ToF s S cerevisiae S pombe SDS SELDI SEM SM sn SPL SREBP TLC ToF ToF-SIM TOR TORC μg μL V v/v Logarithmic Molar Mannosyl diinositolphosphorylceramide mass -to- charge ratio Matrix-assisted ... ionisation Milligram Minute(s) Mannosyl inositolphosphorylceramide Millilitre Millimolar Multiple-reaction monitoring Mass spectrometry Tandem mass spectrometry MS/MS/MS Neutral loss Nanometer ... monitoring approach In preparation 17) Shui G, Guan XL, Low CP, Chua GH, Goh JSY, Yang H, Wenk MR Towards one step analysis of major cellular lipidome using liquid chromatography coupled to mass...
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Development and application of mass spectrometry based proteomics technologies to decipher ku70 functions
... from 10 picomol, picomol, 500 femtomol, 100 femtomol, 50 femtomol, 10 femtomol, femtomol to femtomol with a Mini protein II electrophoresis apparatus (BioRad Laboratories, California, USA) or on ... Silver staining of different amount of BSA picomol, 500 femtomol, 100 femtomol, 50 femtomol, 10 femtomol, femtomol to femtomol BSA (From left to right)were loaded and run by SDS-PAGE and then stained ... (TAP) Tag Pull Down and Mass Spectrometry 23 3.1 Introduction 3.1.1 Mass Spectrometry (MS) In the post-genome era, mass spectrometry (MS) has emerged as a powerful tool to quickly and efficiently...
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Exergoeconomic performance optimization of an endoreversible intercooled regenerative Brayton combined heat and power plant coupled to variable-temperature heat reservoirs
... regenerative Brayton CHP plant coupled to constant-temperature heat reservoirs A thermodynamic model of an endoreversible intercooled regenerative Brayton CHP plant coupled to variable-temperature ... conductance may be different for each heat exchanger because different materials may be used To simplify the problem, the constraint on total heat exchanger inventory is used for the performance optimization ... paper is to optimize the heat conductance allocation among the hot-, cold- and consumer-side heat exchangers, the intercooler and the regenerator, the intercooling pressure ratio and the total pressure...
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Báo cáo khoa học: Structure of Streptococcus agalactiae serine⁄threonine phosphatase The subdomain conformation is coupled to the binding of a third metal ion pptx
... relevant to proteins II Angles at the metal atom, additional weak metal–donor interactions Acta Crystallogr D 56, 857–867 36 Harding MM (1999) The geometry of metal ligand interactions relevant to ... and calculated structure factor amplitudes, respectively Rfree is an R-factor for an unrefined subset of the data (5% of the data) a the metal ions are close to the B-factors of the coordinating ... Moss DS & Thornton JM (1993) PROCHECK: a program to check the stereochemical quality of protein structures J Appl Crystallogr 26, 283 35 Harding MM (2000) The geometry of metal ligand interactions...
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Car Test -- Hydrogen On Demand (Pure hydrogen, not HHO) Can pure hydrogen (H2) replace HHO to increase MPG? pdf
... apparatus is to give a visual indication of hydrogen flow rate during road tests Phillips Company Fill cap Hydrogen input from cell Hydrogen output to solenoid valves (switches to engine or to vent) ... (CC) are provided to the hydrogen- production cell The present invention for hydrogen production improves the state of the art The common method to recover hydrogen from water is to pass electric ... worldwide limit to the fossil fuel available for use in the future In these reforming methods the resulting hydrogen must be somehow stored and delivered to the user, unless the hydrogen generation...
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Báo cáo hóa học: " Mass spectrometry based on a coupled Cooperpair box and nanomechanical resonator system" pdf
... particle adsorbs to the resonator and significantly increases the resonator’s effective mass, therefore, the mechanical resonance frequency reduces Mass sensing is based on monitoring the frequency ... accreted mass according to Equation 19 Conclusion To conclude, we have demonstrated that the coupled NR-CPB system driven by two MW currents can be employed as a mass sensor In this coupled system, ... squeezing by a qubit -coupled nanoresonator Nano Lett 2010, 10:3990 Li JJ, Zhu KD: Plasmon-assisted mass sensing in a hybrid nanocrystal coupled to a nanomechanical resonator Phys Rev B 2011,...
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Báo cáo lâm nghiệp: "Energy consumption of a chipper coupled to a universal wheel skidder in the process of chipping wood" pptx
... the rotor in consequence of the revolution decrease The torque of the rotor consists of the disc torque, shaft torque and cardan shaft torque The other rotating parts are not important due to low ... increase again to n1 This effect is used for constructing the whole power of the motor (Lisičan et al.1996) A Pezzolato H 780/200 overhung chipper coupled to a three-point linkage Zetor 5341 skidder ... are constructed according to experimental conditions (Štempel et al 1964) 36 Fi g C o n n e c t i o n o f the chipper Pezzolato H 780/200 to the skidder Zetor 5341 – torque sensor; – gravimetric...
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Báo cáo khoa học: "Variation in forest gas exchange at to continental scales landscape" ppt
... microclimate profiles, 3) overstory and understory stomatal conductance, and 4) soil water extraction and coupling to soil water stores [4, 9, 19, 40] GCMs have purported to reasonably represent these ... represents a step in the effort to assess current knowledge of forest water balances, to determine how to generalize this knowledge, to include it into simulation models, and to subsequently document ... adapted to water stress and exhibit differing strategies of water use [48], additional studies are required to clarify changes in flux partitioning and changes in total ecosystem gas exchange...
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