... therefore to study in situ andin vitro the expressionand localization of syncytin inhuman placenta at different stages of gestation, in normal and T21-affected pregnancies Results Syncytin is immunolocalized ... cytotrophoblastic cellsand never observed in the syncytiotrophoblast andin the mesenchymal core of the villi (Fig 2A–B) In second trimester placenta (Fig 2C–D), syncytin immunostaining was present: 1 /in the ... cytotrophoblastic cells; 2/ in their thin elongated cytoplasmic processes coming into contact with the syncytiotrophoblast and covering the villus basal lamina At term (Fig 2E–F), immunostaining was detected...
... responses in the PNS [31] These cells may function as antigen-presenting cellsand activate T cellsin vitro in an antigen-specific and MHC-restricted manner [32], especially in the presence of cytokines ... SCs have been implicated inhumanin ammatory demyelinating neuropathies such as ´ Guillain–Barre syndrome and chronic in ammatory demyelinating polyneuropathy [31] In experimental autoimmune ... of SAPK ⁄ JNK in mediating LPS-induced ICAM-1 expressionin SCs JNK phosphorylates c-Jun and ATF-2 and increases their ability to activate transcription, leading to c-jun induction and subsequent...
... formed in metabolically radiolabelled cells The main radioactive oligosaccharides formed in BxPC3 cells (j in lower part, and A, B, and C of upper part) and T5AS clone (h in lower part, and A, B, and ... according to published procedures [9,16,22], with some modifications Total lysates were obtained by boiling 10 in phosphate-buffered saline containing 0.5% SDS and 1.0% 2-mercaptoethanol, and spinning ... cultured in Dulbecco’s modified Eagle’s medium containing 10% foetal bovine serum, 100 UÆmL)1 penicillin, 1.0 mgÆmL)1 streptomycin and mM L-Glu For treating BxPC3 cellsand clones with drugs affecting...
... encoding plasmids and 36 h postincubation the protein fraction was isolated from the cellsand subjected to Western blotting analysis The extent of protein expression is much lower in the HEK293 cells ... scale Constructs containing inactive versions of Rz-1 and Rz-2 were obtained in the same way by using templates as described above but in which the guanosine nucleotides, marked in bold, had been ... expressionin mammalian cells Proc Natl Acad Sci USA 99, 5515–5520 Yu, J.-Y., DeRuiter, S.L & Turner, D.L (2002) RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells...
... the phenotype seen in the neuropathology and neurological disorders such as Alzheimer’s, Parkinson’s and Huntington’s diseases, and brain injury andin ammation, as well as in animal models of ... mitochondrial and nuclear AA content MA-10 cells were labeled for h at 37 °C with [1-14C] AA (1 lCiÆmL)1 per · 106 cells) in serum-free media containing 0.5% fatty acid- free bovine serum albumin Then, cells ... fatty acids present in an aqueous environment by effectively decreasing the insoluble fraction [2] Albumin, in particular, binds specifically to fatty acids [33] In our experiments, we added albumin...
... site that is distinct from the colchicine and vinbastine binding sites in tubulin [10,11] Although benomyl inhibits mitosis in mammalian cells, its antimitotic mechanism of action is not clearly ... beneficial in chemotherapy Similar to taxol and vinblastine, benomyl also induces apoptosis in HeLa cells, indicating that benomyl may be useful in combination with other potent anticancer drugs ... the motor proteins and spindle checkpoint proteins [19] This reduction of checkpoint proteins is required for inactivating the spindle checkpoint signal and progression in the cell cycle [20] The...
... reading frame During the cloning procedure, a cysteine residue was introduced between this signal and the mature poneratoxin Surprisingly, poneratoxin expression was detected only incells infected ... of using it for engineering a baculovirus capable of serving as a bio-insecticide Peptide synthesis andexpression of recombinant poneratoxin Using the published poneratoxin sequence [12], the ... been characterized and nothing is known of the toxin processing during synthesis and secretion into the venom reservoir A peptide was synthesized using the published amino acid sequence [11]...
... Effect of retinoids on interleukin-1-beta (IL-1β)-induced expression of Effect synovial fibroblasts IL-6 in of retinoids on interleukin-1-beta (IL-1β)-induced expression of IL-6 in synovial fibroblasts ... selective retinoic acid receptor (RAR) agonists on inter1 (IL-1)-induced expression of receptor (RAR) agonists on interleukinEffect of selective retinoic acidIL-6 leukin-1 (IL-1)-induced expression ... retinoic (ATRA) on interleukin-6 (IL-6) expression Effect of retinoic acid receptor (RAR) silencing the suppressive effect of all-trans retinoic acidacid (ATRA) on interleukin-6 (IL-6) expression (a)...
... binding sites, or by binding TA to block its DNA-binding or activational domains Interestingly, in two studies reporting that the same promoters were capable of producing binary and graded expression ... the inducer shifts this single peak along the x-axis While observing protein expressionin individual cells is informative for gauging the mode of gene induction, caution should be exercised in ... combinations of parameter values for these factors provide the potential to observe binary, hybrid and graded protein expression Protein expression histograms of β-gal, Luc and GFP In examining...
... problems in signal and image processing, including problems in bioinformatics [31] Second, to extend further the performance of TV minimization, we have introduced a novel strategy for using multiple ... been chosen correctly If, in addition, v(x) is inde- will be uniquely defined for any partitioning, meaning that finding u* is reduced to finding an optimal partitioning of I which can be performed ... moderate in comparison with the intrinsic variability in gene expression Third, the expression of some genes may not be influenced by the underlying genomic change For example, copy number gains are...
... cell content from simulated data using segmentation Tumor cells (%) Tumor cells using hemizygous loss (%)* Tumor cells using single copy gain (%)† Tumor cells using copy neutral LOH (%)‡ Paired ... homozygous deletion in all investigated cells no genetic material remains and the BAF estimates become essentially random due to the low SNP signal intensity [22] With an increasing fraction of normal ... hybridizations on Illumina HumanHap300 version Genotyping BeadChips representing one colon cancerand two breast cancer tumors, with matched normal samples (Courtesy of Illumina Inc., San Diego, CA,...
... myosin, gelsolin, filamin, spectrins, microtubular proteins including tubulins, microtubule associated proteins, and intermediate filament proteins including vimentin keratin and nuclear lamins ... genes involved in lipid use and energy balance (AMP-activated protein kinase-gamma3, uncoupling protein (UCP)-3, GLUT4 in the case of silencing NR4A1 and FABP4, CAV3, UCP2 in the case of silencing ... elements that contribute to its instability three repeats of ‘ATTTA’ inits 3’ non coding sequence and a sequence rich in proline, glutamic acid, serine and threonine (PEST sequence) which is...
... in the AUH gene This indicated that AUH, in addition to its RNA binding function, must play an important role in leucine catabolism AUH was overproduced in E coli and characterized by measuring ... (c.719C>T) in exon and an insertion mutation c.613–614insA This insertion causes a frameshift that starts at Met205 and leads to the introduction of a stop codon after four amino acids The intra-assay ... to be involved in leucine degradation since MTP deficiency (MIM 143450, MIM 600890) is not associated with increased urinary excretion of 3-methylglutaconic acid A protein was purified from human...
... linkages of insulin have been shown to be important in maintaining its native conformation and biological activity [20–25] The double-chain insulin is synthesized in vivo as a single-chain precursor ... (preproinsulin) and folded as proinsulin, in which a connecting peptide of 35 residues links the C terminus of the B chain and N terminus of the A chain After digestion by a specific set of protein ... protein enzymes in the B-cell granule, proinsulin is converted into insulin and C-peptide of 31 amino acids [26] Previous studies completed on the unfolding process of insulin or proinsulin were often...
... HylP2 and ascorbic acid at three binding regions in the polysaccharide binding groove Table Hydrogen bonded interactions between HylP2 and lactose at three binding regions in the polysaccharide binding ... present study provides insight into the inhibitor binding sites in HylP2 and postulates the substrate binding regions in the bacteriophage enzyme as a result of ascorbic acid being structurally similar ... polysaccharide binding site in HylP2 The interactions between protein residues and ligand molecules are indicated by dotted lines Subsite Fig (A) The 3D structure of HylP2 showing each monomer chain in three...
... human proteins are shared; and 9, whether there is a textmined interaction between the human proteins An NBC must be trained using positive and negative training data sets in order to determine ... modules are acting cooperatively in establishing and/ or maintaining the GBM phenotype, and suggests that the development of GBM cancers involve malfunctions in both signaling transduction and cell-cycle ... pathways, in contrast to breast cancerand GBM, which show no such enrichment Genes in module are involved in focal adhesion and integrin pathways, while genes in module are involved in DNA repair...
... as a continuous sinusoidal staining andin the stroma of portal tracts (Fig 7A) In the cirrhotic liver, the sinusoidal staining was enhanced, whereas a very faint staining was observed in fibrous ... LIF-M expressionin various cell lines andinhuman liver RT-PCR analysis of LIF-M expressionin various cell lines andinhuman liver (A): LIF-M expression was analyzed with the hLIF-M2 and hLIF-3N ... case of s-LIF-D, initiation at the AUG within exon D would result in a reading-frame shift following the 6th amino -acid (aa) and a termination at aa 88, the resulting protein bearing no homology...
... caffeine is able to potentiate the production of proinflammatory cytokines both in vitro [25,26] andin vivo [27] and that caffeine exacerbates tissue injury during inflammation [5,24] In contrast ... the inflammatory response and organ injury are reduced AMPD1, adenosine monophosphate deaminase induced by experimental human endotoxemia increase the circulating adenosine concentration in vivo? ... (a) inflammation-induced cytokine release and (b) tissue injury However, in the presence of caffeine, a non-selective adenosine receptor antagonist, this mechanism of protection is lost and inflammation-induced...
... and satisfactory filling [Tables and 2] DISCUSSION Through its complex interactions with matrix components and cells, HA has multifaceted roles in biology utilizing both its physicochemical and ... distally, and lingually Data were obtained at baseline before treatment and at 10 days, and 6,9, and 24 months after treatment Surgical Technique After local anaesthesia, intrasulcular incisions ... showed good results in manipulating and accelerating the wound healing process in a large number of medical disciplines, as evident in ophthalmology, dermatology, dentistry and rheumatology [10,11]...