... Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections Virol ... SARS corona virus, yellow fever virus, human herpes virus6 , camelpox virus, CMV [7] and Epstein-Barr virus (EBV) [12] However such a study has never been carried out using the key human pathogens ... plates in a final volume of 25 μl under the following cycle conditions: 50°C for minutes, 95°C for minute, 45 cycles of 95°C for 15 seconds 55°C for 30 seconds and 72°C for 30 seconds Data analysis...
... Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections Virol ... SARS corona virus, yellow fever virus, human herpes virus6 , camelpox virus, CMV [7] and Epstein-Barr virus (EBV) [12] However such a study has never been carried out using the key human pathogens ... plates in a final volume of 25 μl under the following cycle conditions: 50°C for minutes, 95°C for minute, 45 cycles of 95°C for 15 seconds 55°C for 30 seconds and 72°C for 30 seconds Data analysis...
... 5C-AxxA virus and the initial dose of 5C virus (p = 0.5) Equivalent MOIs were therefore achieved using equal RT units of wild type and mutant Nef bearing virus and 5-fold more RT units of the ... p24 concentration of SF2 virus - peak p24 concentration of Nef)/peak p24 concentration of SF2 virus replication versus CD25 MFI of the CD3+ population Spearman correlation was performed using GraphPad ... molecular analysis of an active humanimmunodeficiencyvirus type Nef/p21-activated kinase complex J Virol 2005, 79(20):12732-12741 Renkema GH, Manninen A, Saksela K: Humanimmunodeficiencyvirus type...
... great potential of current knowledge to modulate levels of these important phytonutrients for the benefit ofhuman health and will, therefore, be separately discussed in a later part of this chapter ... flavonoids impart much of the color and flavor of fruits, vegetables, nuts, and seeds, they form an integral part of the human diet (Parr and Bolwell, 2000) Rich dietary sources of flavonoids include ... five of the above color groups can be assigned to the carotenoid or flavonoid families of phytonutrients, underlying their importance forhuman nutrition Some members of the carotenoid family of...
... objective of this study is to evaluate the psychometric properties of the Child-OIDP for use among children in UK In addition, we will also report on the prevalence of oral impacts in a sample of 10–11 ... empirically tested before and after the pilot study The frequency distribution for the Child-OIDP scores is skewed and hence non-parametric testing was applied Consequently, testingfor concurrent ... In terms of internal reliability analysis, the majority of corrected item-total correlations were above the Results Page of (page number not for citation purposes) Health and Quality of Life Outcomes...
... process of evaluating HRQoL measures consists of two stages; the first involves an assessment of the reliability and validity and the second consists of on-going evaluations of the performance ... (CPQ11-14) [22] These formed the components of the Child Oral Health Quality of Life Questionnaire that had been designed to assess the impact of oral conditions on the QoL of children and adolescents ... both self-completed Items of the CPQ used Likert-type scales with response options of “Never” = 0; “Once or twice” = 1; “Sometimes” = 2; “Often” = 3; and “Very often” = For the CPQ11-14, the recall...
... were performed for the VAS and TTO data to estimate the effect of the different domains, the effect of being member of one of the two patient groups, and the effect of the assessment of the patients’ ... error of means) of the set of EuroQol-5D health states derived by three different measurement methods (ranking, VAS, TTO) presented for the general population and for the two patient groups (For ... calculated for the background characteristics of the three samples Then frequency distributions were made for the classification of the patients’ health state Mean scores and standard errors of the...
... HMA was originally described for analyzing the sequence heterogeneity of the envelope gene ofhumanimmunodeficiencyvirus (HIV) [23,24] HMA involves hybridization of a radioactive probe generated ... for increased HMR for Taq amplified HALT-C samples as well as for HMR and complexity scores for HCV/HIV coinfected samples Based on the current results, it would Page of 11 (page number not for ... the National Centerfor Minority Health and Health Disparities and by General Clinical Research Center grants from the National Centerfor Research Resources, National Institutes of Health (grant...
... a powerful resource for inferences of demographic history So far, resequencing efforts of smaller subsets of the human genome have already yielded a comprehensive portrait of historical changes ... exome data One of the most promising applications for exome data is the study of natural selection in humans [13] Inferring patterns of natural selection on genes is a powerful approach for gauging ... unknown whether these more complex forms of balancing selection have an important role in the patterns ofhuman genetic diversity, and exomes are ideal for this line of inquiry because their cost-effectiveness...
... Expression of GPCR mRNA in various types ofhuman cells detected by RT-PCR Expression of GPCR mRNA in various types ofhuman cells detected by RT-PCR (A) Relative amount of mRNA expression for CCR5, ... sequences of functional clones of the AIDS virus AIDS Res Hum Retroviruses 1987, 3:57-69 Cheng-Mayer C, Quiroga M, Tung JW, Dina D, Levy JA: Viral determinants ofhumanimmunodeficiencyvirus type ... and characterization ofhumanimmunodeficiencyvirus type variants infectious to brain-derived cells: detection of common point mutations in the V3 region of the env gene of the variants J Virol...
... Strebel K: Augmentation ofvirus secretion by the humanimmunodeficiencyvirus type Vpu protein is cell type independent and occurs in cultured human Page 14 of 15 (page number not for citation purposes) ... membrane-spanning domain of the humanimmunodeficiencyvirus type Vpu protein Virology 1998, 251:96-107 Gottlinger HG, Dorfman T, Cohen EA, Haseltine WA: Vpu protein ofhumanimmunodeficiencyvirus type enhances ... responsible for the ubiquitination of proteins destined for degradation by the proteasome and the enhanced CD4 turnover in presence of Vpu Indeed, further lines of evidence for an involvement of the...
... that are essential forhumanimmunodeficiencyvirus type replication J Virol 1996, 70:651-657 Petit C, Schwartz O, Mammano F: The karyophilic properties ofhumanimmunodeficiencyvirus type integrase ... flap in humanimmunodeficiencyvirus type nuclear import J Virol 2002, 76:12087-12096 Limon A, Devroe E, Lu R, Ghory HZ, Silver PA, Engelman A: Nuclear localization ofhumanimmunodeficiencyvirus ... Assessment of the role of the central DNA flap in humanimmunodeficiencyvirus type replication by using a single-cycle replication system J Virol 2004, 78:3170-3177 Poon B, Chen IS: Human immunodeficiency...
... posttransfection were analyzed forvirus release by measuring p25 and vector RNA genome copies and for competence for transduction in 293T cells The efficiency of transfection was similar for all plasmid combinations ... optimized feline immunodeficiencyvirus vectors Mol Ther 2000, 1:31-38 Poeschla EM, Wong-Staal F, Looney DJ: Efficient transduction of nondividing human cells by feline immunodeficiencyvirus lentiviral ... characterization of attenuated feline immunodeficiencyvirus long terminal repeat mutant viruses J Virol 2001, 75:1054-1060 Whitwam T, Peretz M, Poeschla E: Identification of a central DNA flap in feline immunodeficiency...
... Ministry of Health and Social Welfare for offering me a scholarship for my Master of Public Health Programme Special thanks go to my teachers of Umea International School of Public Health, for consolidating ... (Ackah et al, 1995) (CD4 cells are type of lymphocyte cells (white blood cells) that form an important part of the immune system HumanImmunodeficiencyvirus most often infects these cells) (MoHSW ... are known to be an important cause of reactivation of the TB infection In recent years TB has become prevalent among people living with humanimmunodeficiencyvirus (HIV) which poses a major public...
... Harlem Hospital Center, SUNY Health Sciences Center at Brooklyn, Mt Sinai Medical Center, University of Southern California, Bronx-Lebanon Hospital Center, Columbia-Presbyterian Medical Center, New ... Hispanic; and 34% had a history of injection drug use Sputum smears for AFB were positive at baseline for 69% Only five (4%) had a history of treatment for a previous episode of tuberculosis The median ... Harris for assistance in data analysis; Laura Liberman and Donna Mildvan for critical review of the manuscript; the Mycobacteriology Clinical Reference Laboratory at the National Jewish Center for...
... (data not shown) For prevention of MTCT of HIV-1, highly active antiretroviral therapy was recently Page of recommended for all women with HIV-1 RNA levels of ≥ 1000 copies/ml of plasma [13] A ... tool for the analysis of association studies Bioinformatics 2006, 22:1928-1929 13 Jamieson DJ, Clark J, Kourtis AP, Taylor AW, Lampe MA, Fowler MG, Mofenson LM: Recommendations forhumanimmunodeficiency ... innate immunity might be useful for future treatment of pediatric HIV-1 infection and AIDS List of abbreviations CI: confidence intervals; HIV-1: HumanImmunodeficiencyVirus type 1; MTCT: mother-to-child...
... of heterologous retroviruses J Virol 1996, 70:8285-8300 Ritter GD, Yamshchikov G, Cohen SJ, Mulligan MJ: Humanimmunodeficiencyvirus type glycoprotein enhancement of particle budding: role of ... Mutational analysis of the humanimmunodeficiencyvirus type Vpu transmembrane domain that promotes the enhanced release of virus- like particles from the plasma membrane of mammalian cells J ... PB: Molecular dynamics investigation of membrane-bound bundles of the channel-forming transmembrane domain of viral protein U from the humanimmunodeficiencyvirus HIV-1 Biophys J 2002, 83:1259-1267...
... significant production of HCV particles occurs in vitro The claims of levels ofvirus titers for many human viruses are merely claims There are no definitive and reproducible methods for titrating biologically ... Human herpesvirus 6A decreases the susceptibility of macrophages to R5 variants ofhumanimmunodeficiencyvirus 1: possible role of RANTES and IL-8 Virus Res 2006, 121(2):161-168 Radkowski M, Wilkinson ... among these viruses at the level of a single cell is important Our experience in human viruses allowed us to develop this system We realize that the occurrence of triple infections of single cells...