... numbering accord-ingly), and found to be 3748 nucleotides in length. A TATA-box (TATAA) was located at nucleotides 83–87 and a poly (A) sequence ATTAAA at nucleotides2978–2983. A GC-rich area was 107 ... needle,as previously described [28]. The isolated DNA wasextracted with phenol and chloroform, precipitated withethanol and Na-acetate, and resuspended into water. Totalcellular DNA was also alternatively ... 3177–3182.4 Okamoto H, Nishizawa T, Kato N, Ukita M, Ikeda H,Iizuka H, Miyakawa Y & Mayumi M (1998) Molecularcloning and characterization ofa novel DNA virus(TTV) associated with posttransfusion...
... Theeluted mAbs and mAb-FIs were concentrated and stored at-80°C.Analytic characterization of proteinsThe protein concentration of mAb and mAb-FI sampleswas determined by measuring the optical density ... presence and absence of a reducing agent (5 mM 1,4-dithiotreitol) and staining withCoomassie brilliant blue. The aggregate content of mAb and mAb-FI samples was analyzed by high-performanceSEC ... separate plasmids. Expression of all antibody and antibody-FI light and heavy chains is controlled by a shortened intron A- deleted immediate early enhancer and promoter from the human cytomegalovirus...
... sensitivity of the stochastic model as a function of frequency. This was done using Equation 7 and a Gaussian external force that was band-limited to 200 Hz and had a standard deviation of 1 pN. For eachchoice ... electrical oscillator found in saccular hairas an oscillatory module capable of nonlinear amplification. This further supports theidea of nonlinear oscillators playing a crucial role in the operation ... contributionsAS, LH and AN carried out simulation and bifurcation analysis of the deterministic model. AN carried outcalculation of the Lyapunov exponent. KD and BL carried out numerical calculations of...
... reaction calorimetry were fitted toa mathematical model based on mass and energy balances for a batch reactor coupled by the reaction rate equation. This reaction rate was modeled as: a) a ... using heat flux data measured with a reaction calorimeter. This approach to kinetics study was based on the coupling of the reaction rate with mass and energy balances. The heat data obtained ... experimental data in order to estimate the reaction rate constant, k, and the order of the reaction, m. The application of reaction calorimetry to obtain kinetic data from reactions was found to be...
... (financial incentives; audit and feedback)Oct Audit and feedback report #1Feb Audit and feedback report #2Jun Audit and feedback report #3Oct Audit and feedback report #4AprAudit and feedback ... Council of Teaching Hospitals (COTH)directory or if the American Medical Association’s (AMA) Fellowship and Residency Electronic Interactive Database (FREIDA) database listed the VA facility ashaving ... implementation: hospitaldirector approval; Institutional Review Board (IRB) and VA Researchand Development (R&D) approval; having a credentialed individual willing to serve as the site’sPI;and...
... necrosis and perforation [2].Case presentation A 68-year-old Caucasian man presented to our hospitalwith acute, severe abdominal pain. An examination of the patient revealed that he was septic and ... anda high C-reactiveprotein level at 208 mg/L. The patient had normal renalfunction anda normal hemoglobin level. An abdominalradiograph revealed dilated small bowel loops, and a clinical ... diverticulum: a rare cause of acute abdominal pain. Ann Emerg Med 1987, 16:811-812.2. Limas C, Seretis K, Soultanidis C, Anagnostoulis S: Axial torsion and gangrene ofa giant Meckel’s diverticulum. J Gastrointestin...
... strata: theory and practical application. Journal of Clinical Pharmacy and Therapeu-tics 1 A. D 26:121-128.Additional file 1Candidate factors by change stages. Table lists 33 candidate factors ... Canada, 2Department of Paediatrics, University of Toronto, Toronto, Canada, 3Department of Health Policy, Management and Evaluation, University of Toronto, Toronto, Canada, 4Centre for Health ... http://www.implementationscience.com/content/4/1/32Page 8 of 8(page number not for citation purposes)4. Hagino A, Hamada C, Yoshimura I, Ohashi Y, Sakamoto J, NakazatoH: Statistical comparison of random allocation methods incancer clinical...
... strata: theory and practical application. Journal of Clinical Pharmacy and Therapeu-tics 1 A. D 26:121-128.Additional file 1Candidate factors by change stages. Table lists 33 candidate factors ... Canada, 2Department of Paediatrics, University of Toronto, Toronto, Canada, 3Department of Health Policy, Management and Evaluation, University of Toronto, Toronto, Canada, 4Centre for Health ... randomization and stratification of patients to clinical trials. J Chronic Dis 1974, 27:365-375.8. Kenjo Y, Antoku Y, Akazawa K, Hanada E, Kinukawa N, Nose Y: Aneasily customized, random allocation...
... polytopeSuyoung Choi∗Department of Mathematical SciencesKAIST, Republic of Koreachoisy@kaist.ac.kr(Current) Department of MathematicsOsaka City University, Japanchoi@sci.osaka-cu.ac.jpJang ... combinatorics 17 (2010), #R9 1 For a d-dimensional simplicial polytope P , we can attach a d-dimensional simplex to a facet of P . A stack ed polytope is a simplicial polytope obtained in this way ... (3)Herzog and Li Marzi [4] gave another proof of (3).The main purpose of this paper is to prove (3) combinatorially. In the meanwhile, weget as corollaries the results of Bruns and Hibi [2] : a formula...
... sepa-rate independent advice for each party is always a redflag toa judge. On a practical note, having separatelegal advisors can help you and your fiancé craft a lasting agreement that you ... research efforts of Ella Hirst. Thanks also to TomHannah for his work on the preliminary research. We are indebted to Lisa Guerin for her editorial assistance, andto AndréZivkovich for his sharp-eyed ... having a premarital agreement is a good way to do this. What do you think? Can wetalk about it? I’ve got a good book about prenups, and a few suggestions and ideas. Let’s arrange a time to take...
... Much of this information can be handled within a graphical format, such as tables and graphs, though a paragraph explanation of each is generally required. Make sure to include total dollar (or ... business plan, such as a new business proposal to the financial community. There are many ways to develop and format a marketing plan . The approach taken here is to present a 6-Part plan that includes: ... Other Areas Preferably this section includes a brief summary of current marketing decisions (see Situational Analysis) so readers of the plan can easily compare what was planned to what is planned....
... than the appearance. In many cases, a reasonably neat diagram can be drawn by hand faster than with a graphics package, and you can scan it if you want an electronic version. Either is usually ... reliable and more awkward to use as they acquire more features. This is a general feature of commercial software and an important input to the computing industry. If software and operating ... information and arguments as a series of numbered points, rather than as one or more long and awkward paragraphs. A list of points is usually easier to write. You should be careful not to use...
... self-delusory approach to corporate image brochures are plain to see: high costs and low value. But it doesn't have to be this way. Some years ago, I was commissioned towritea corporate image ... for a pharmaceutical company. When it was printed, not only did people read it, they actually called the company to request additional copies to give to friends, clients, and professional colleagues. ... can assume that "everyone will want to read what I am going to write& quot;. This is the "creative writing attitude". The purpose of expository writing is usually to inform and...
... three supporting paragraphs that follow.PREPARED BY SANDRA DANIEL HowToWrite An Effective Five Paragraph Essay PREPARED BY SANDRA DANIEL Topic: The death penalty should be abolished. Do ... comparison.Body Paragraphs – part 2PREPARED BY SANDRA DANIEL Thesis Statement – part 1An effective thesis statement should make a strong assumption about the topic and serve as a map for the rest of the ... move toa broader focus.Introduction - start with a narrow focus and end your conclusion with a broad statement about the topic.An effective five paragraph essay shows not only a writer’s ability...