historical perspective development of nif genetics and regulation in klebsiella pneumoniae
Báo cáo y học: "Post-translational aging of proteins in osteoarthritic cartilage and synovial fluid as measured by isomerized aspartate" pot
... assumptions of normality, linearity, and homoscedasticity essential to methods of linear regression modeling, we logarithmically transformed IsoAsp, GAG, and protein values and used the D'Agostino and ... protective role in preventing degradation of collagen fibrils [28] With increasing loss of proteoglycan, there is increasing degradation of collagen and other matrix components [29], and this is ... Aswad DW: In vitro aging of calmodulin generates isoaspartate at multiple Asn-Gly and Asp-Gly sites in calcium-binding domains II, III, and IV Protein Sci 1993, 2:1648-1663 Sandmeier E, Hunziker...
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Post translational regulation of the human TRIP br1 cell cycle protein
... regulated Unable to induce S-phase in quiescence cells Mainly involved in the regulation of differentiation and development • Lacks the domains involved in transactivation and binding to the pRB family ... resulting in the assembly of an E2F-1/DP1/TRIP-Br1 ternary complex PHD zinc finger- and/ or bromodomain-containing proteins such as p300/CBP and KRIP1 compete for binding to TRIP-Br proteins and ... showing the functional domains and phosphorylation events impinging on MDM2 [60] The amino acid residues are numbered Functional domains of the MDM2 protein including the p53 binding domain,...
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Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx
... start of exon resulting in loss of the AUG initiating translation of the 88 kDa isoform We specifically point out that differential utilization of exon vs exon in splice variants introduces differing ... staining patterns (comparing and 2) and by the yellow color resulting from combination of green and red fluorescence in that iPLA2c is localized, in large part, to the mitochondrial compartment in ... Translational regulation of iPLA2c in myocardium Owing to the obvious complexity of the regulation of iPLA2c resulting from the combined presence of transcriptional and translational regulation, ...
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Tài liệu Báo cáo khoa học: Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form ppt
... approximate regions of interacting surfaces A, C and D are indicated on the top line Charged residues which are possibly involved in interactions at the D surface are indicated in bold and the external ... Caspar [33] in 16 turns and enables indexing of the power spectrum (Fig 2E, insert) as shown in Fig 3A The clear diffraction spot with a spacing of 7.7 nm is interpreted as a Bessel function of order ... mL of Luria–Bertani broth containing 25 lgÆmL)1 kanamycin and 200 lgÆmL)1 chloramphenicol This was grown overnight and then diluted into L of Luria–Bertani broth containing 25 lgÆmL)1 kanamycin...
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Báo cáo Y học: Regulation of RAS in human platelets Evidence that activation of RAS is not sufficient to lead to ERK1-2 phosphorylation pot
... using GST fusion protein containing the RAS-binding domain of RAF Precipitated proteins were resolved by 12.5% SDS/PAGE and analysed by Western blotting using an anti-RAS Ig (top) Proteins of ... using GST fusion protein containing the RAS-binding domain of RAF Precipitated proteins were resolved by 12.5% SDS/PAGE and analysed by Western blotting using an anti-RAS Ig (top) Proteins of ... Thombin, convulxin and TPO induce activation of RAS RAS activation was measured through the ability of its activated form (RAS–GTP) to bind to a GST fusion protein consisting of the RAS-binding...
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Báo cáo khoa học: Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains pot
... Proteomics-based identification of novel factor inhibiting hypoxia-inducible factor (FIH) substrates indicates widespread asparaginyl hydroxylation of ankyrin repeat domain-containing proteins Mol Cell Proteomics ... uptake repressor, PerR in Bacillus subtilis, which is inactivated by oxidation of the imidazole rings of two Fe(II)-binding histidinyl residues resulting in suppression of peroxidedefence genes ... Gln 239FIH) Instead the imidazole ring is sandwiched between the side chains of His 199FIH that forms part of the catalytic triad and the aromatic ring of Tyr 102FIH The side chain of Gln 239FIH...
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Báo cáo sinh học: "Regulation of metabolism in Caenorhabditis elegans longevity" ppsx
... Possible link between accumulation of amino-acid pools, mitochondrial function, and the insulin and TOR pathways in C. elegans longevity Black lines indicate the activating or repressive function of ... in amino acid levels, especially branched-chain amino acids Falk et al [9] showed that mutations affecting complexes I, II and III of the electron transport chain (ETC), including some that increase ... effector of the TOR pathway and animals carrying a mutation in this gene also live longer; and ife-2 mutants accumulate pools of amino acids that are known to induce protein biogenesis and inhibit...
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Báo cáo y học: "Expression and regulation of CCL18 in synovial fluid neutrophils of patients with rheumatoid arthritis" potx
... most chemokine receptors were downregulated in SF PMN, supporting the notion of a differential modulation of chemokine receptors and their ligands during chronic inflammation These findings suggest ... contaminating eosinophils accounted for CCL18 production Notably, no eosinophils were detectable in preparations of SF PMN Cell culture For culturing PMN, RPMI 1640 containing L-glutamine and sodium ... expression in SF PMN occurs as a result of the recruitment of PMN into the inflammatory milieu of the joint Differential expression of chemokine receptors in SF PMN To estimate changes in the responsiveness...
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Báo cáo y học: "Expression and regulation of neurotrophins in the nondegenerate and degenerate human intervertebral disc" pdf
... staining for Trk-A and Trk-B was largely cellular in the chondrocyte-like cells of the NP and IAF, and also in the fibroblast-like cells of the OAF (Figures 4c,d and 5c,d) Positive staining in ... burden of back pain in the UK Pain 2000, 84:95-103 Freemont AJ, Le Maitre C, Watkins A, Hoyland JA: Degeneration of intervertebral discs: current understanding of cellular and molecular events, and ... increase in NGF protein and gene expression in NP cells seeded in monolayer and treated with IL-1β [19] Indeed a number of studies have demonstrated regulation of NGF expression and NGFassociated pain...
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Cyclic nucleotides and mitogen-activated protein kinases: regulation of simvastatin in platelet activation pptx
... prostaglandin E1 (PGE1) (0.5 μM) and heparin (6.4 IU/ml) The washed platelets were finally suspended in Tyrode's solution containing bovine serum albumin (BSA) (3.5 mg/ml) The final concentration of ... Simvastatin (30 and 50 μM) did not significantly affect FITC-triflavin binding to the αIIbβ3 integrin in platelet suspensions (c, 4.8 ± 0.1; d, 4.9 ± 0.1) (Fig 1C), indicating that the inhibitory ... d Figure Effects of simvastatin on the inhibition of (A and B) platelet aggregation, (C) FITC-triflavin binding to the αIIbβ3 integrin and (D) [Ca2+]i mobilization in activated platelets Washed...
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Báo cáo y học: "Stanniocalcin-1 promotes tumor angiogenesis through up-regulation of VEGF in gastric cancer cells" ppt
... tumor in the flank of nude mice (C) Immunohistochemical staining of STC-1 in tumor tissues of nude mice STC-1 was detected on the membrane of tumor cells (D) Immunohistochemical staining of PCNA in ... sections from individual mice and three independent in vivo experiments (F) Hematoxylin and eosin stained sections of the Matrigel plugs (E, endothelial-like cells; T, tumor cells; S, surrounding tissues; ... plate containing 500 μl tumor supernatants 12 h later, the inserts were removed and washed with PBS, fixed, stained, rinsed with water, and photographed in random fields (400×, or 200×) per insert...
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Báo cáo y học: " Post-translational modifications of histones H3 and H4 associated with the histone methyltransferases Suv39h1 and G9a" pdf
... likely influence subsequent modifications, regulated by both cis and trans mechanisms Characterizing such modifications could provide insight into the roles of chromatin-binding proteins In this ... these HMTs could help in understanding the in vivo state of constitutive heterochromatin associated with Suv39h1, and that of the silent euchromatin and facultative heterochromatin associated with ... lysines and with the amino termini of proteins Gel slices were treated for h at 37°C with 100 μl of 30% propionic anhydride in methanol and 40 μl of 50 mM NH4HCO3 [24-26], followed by two ten-minute...
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Báo cáo y học: "Global nucleosome distribution and the regulation of transcription in yeas" doc
... segregation of intergenic regions of the genome into the aqueous phase Nagy et al [13] proposed that this may be a result of different efficiency of chromatin cross-linking along the genome and that ... change in nucleosome occupancy in a typical yeast gene in different transcriptional states (a) When there is no transcription, repressor proteins bind to their DNA-binding sites and maintain a ... remains bound [9] Together, these observations suggest that Rap1p binding alone is insufficient to keep nucleosomes off promoters, and it probably requires additional cofactors and/ or chromatin-remodeling...
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Báo cáo y học: "Translational regulation of gene expression" docx
... localization of this mRNA in fibroblasts and neurons, but also its translation He showed that binding of ZBP1 probably inhibits translation by interfering with the formation of the 80S ribosome during initiation ... phosphorylating ZBP1 are involved in this regulation Serine phosphorylation of ZBP1 by extracellular-regulated kinases (ERKs) induces repression of translation In contrast, tyrosine phosphorylation of ... Robert Singer (Albert Einstein College of Medicine, New York, USA), described how the trans-acting factor zipcode-binding protein (ZBP1), which binds to the 3Ј UTR of -actin mRNA, regulates not...
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REGULATION OF CHEMICALS IN EUROPE – REACH
... environment Maintenance and enhancement of the competitiveness of the EU chemical industry Prevention of fragmentation of the internal market Increased transparency Integration with international ... human intake No implicit assumption about a ”safe” intake 37 INTERPRETATION OF MARGIN OF EXPOSURE • Inter-species and intra-species differences • 100 would be sufficient • Nature of the carcinogenic ... human intake • Estimation of human exposure • Calculation of an MOE 36 MARGIN OF EXPOSURE (MOE) Definition: The ratio between the defined point on the dose-response curve for the adverse effect and...
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Understanding the regulation of cytokinesis in fission yeast
... Assembly of the actomyosin ring in fission yeast 11 1.3.1.3 Signaling pathways invovled in cytokinesis 14 Temporal regulation of cytokinesis 19 1.3.2.1 Cell cycle regulation of cytokinesis in metazoans ... proteins participate in cross-linking and/ or stabilization of F-actin, and anchor the actomyosin ring to plasma membrane Together, they organize F-actin and myosin II into a dynamic contractile ring ... cytokinesis in mammalian cells (Mukhina et al., 2007; Reichl and Robinson, 2007) Excessive alpha-actinin prevents actin turnover and inhibits cytokinesis; in contrast, depletion of alpha-actinin...
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Role and regulation of juxtanodin in actin cytoskeleton of oligodendrocyte
... proteins (Turunen et al., 1994) In addition to this domain, two more actin-binding domains capable of binding F-actin and G-actin have been identified in N-terminal and middle regions of ezrin ... C-terminal actin-binding domain The identity of amino acid sequence with ezrin in each domain is indicated As shown in Fig 1.4, the amino acid sequences of ERM N-terminal membrane-binding domain are ... presence of two F-actin binding domains (Drenckhahn et al., 1988) However, α-actinin forms the more loosely ordered actin structure of stress fibers α-actinin has a single actin-binding domain in its...
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Regulation of cytokinesis in the fission yeast schizosaccharomyces pombe
... ring producing two daughter cells of equal size The actomyosin ring consists of about 50 different proteins including actin, actin regulatory proteins, type II myosin heavy and light chains The ... number of proteins, which are essential for the formation and maintenance of the actomyosin ring They include the myosin 16 heavy chain Myo2p; essential myosin light chain Cdc4p; myosin assembly ... are formed but ring constriction fails The actomyosin ring disassembles and continuous rounds of nuclear division occur in the absence of cytokinesis resulting in formation of multinucleated cells...
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THE ROLE OF THE CTD PHOSPHATASE RTR1 AND POST-TRANSLATIONAL MODIFICATIONS IN REGULATION OF RNA POLYMERASE II
... staining and removal of proteins isolated in the gel, making this method both time consuming due to the need to excise individual spots of protein from the gel and limiting in the number of low ... Phosphorylated serine of yeast RNA polymerase II C-terminal domain S7 Serine of yeast RNA polymerase II C-terminal domain S7-P Phosphorylated serine of yeast RNA polymerase II C-terminal domain S cerevisiae ... repeats (described in detail below and reviewed in Heidemann, Hintermair, Voss, & Eick, 2013) In addition, modifications of interacting protein factors aid in the regulation of the transcription...
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