histopathologic genetic and molecular considerations of non clear cell renal cell carcinoma
Genetic and molecular dissection of DELLA related GA signaling pathway in regulating arabidopsis seed germination
... OF MOLECULAR AND CELL BIOLOGY DEPARTMENT OF BIOLOGICAL SCIENCE NATIONAL UNIVERSITY OF SINGAPORE 2007 Acknowledgements I gratefully acknowledge the Institute of Molecular and Cell Biology (afficiated ... bacteria with plasmids 43 3.6.5.1 Preparation of E.coli competent cells for heat-shock transformation 43 3.6.5.2 Transformation of E.Coli cells using heat-shock method 43 3.7 DNA sequencing 44 3.8 Plant ... metabolic activity in ga1-3 mutant 117 5.2.6 Distinct approaches are utilized to control cell growth and cell wall modification during seed germination and floral development 118 5.2.7 DELLAs act...
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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... mgÆmL)1 l-arabinose for induction of GroEL–GroES After incubation at 37 °C until the cells reached an A600 of 0.6, the cells were induced with 0.5 mm isopropyl-b-d-thiogalactopyranoside and cultured ... Pseudomonas fluorescens PfO-1 (HYDPf) were able to hydrolyze d,l-p-HPH SDS–PAGE analyses of wholecell extracts and the supernatant and precipitate fractions are shown in Fig It was found that E ... formation of inclusion bodies in the precipitate fraction, which may lead to low activity of whole -cell extract, while HYDBp and HYDPf were mainly expressed in soluble fraction under the experimental...
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Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf
... 2003 Fig Phylogenetic tree of hazelnut LOXs The amino acid sequences of hazelnut lipoxygenases were compared with other plant LOXs (accession numbers are in parentheses) The phylogenetic tree ... mitotic activity and the consequent rapid remodelling of cell membranes occurring in developing seed FASTA analysis suggests that, despite the clear evidence for expression in early seed development, ... Despite these clear indications on the importance of 9-LOXs in plant defence, the nature and the biological activities of oxylipins produced from 9-hydroperoxides are still largely unclear Recently...
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MORPHOGENESIS THE CELLULAR AND MOLECULAR PROCESSES OF DEVELOPMENTAL ANATOMY pptx
... structures of multicellular organisms may be traced back to the few modi operandi of cell growth, cell evanescance (Zellenscwund), cell division, cell migration, active cell formation, cell elimination ... the extracellular environment, the cell membrane and the intracellular cytoskeleton Chapters and describe the morphogenetic properties of fibroblasts and epithelia, the two main types of cells found ... include both other cells and extracellular matrices, as well as the macroscopic boundaries that constrain cell activity As to the interactions among the cells participating in the morphogenetic enterprise,...
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Báo cáo khoa học: Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus doc
... approach [22,49], they have not been confirmed so far in H pylori cells As for other T4SS, it is currently also unclear whether the extracellular pilus-like appendages are used as conduits for protein ... into the host cell cytoplasm is not understood Incubation of target cells with different inhibitors interferes with CagA tyrosine phosphorylation [36,46], although it remains unclear whether ... effector protein CagA into various host cells is a hallmark of CagT4SS activity Despite the molecular characterization of a number of effects in host cells, the exact function of CagA translocation...
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Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx
... laccase and cellulase genes in Lentinula edodes on a sawdust-based substrate Mycoscience 41, 149–153 56 Cai, Y.J., Buswell, J.A & Chang, S.T (1994) Production of cellulases and hemicellulases ... J.A & Chang, S.T (1999) Production and distribution of endoglucanase, cellobiohydrolase, and b-glucosidase components of the cellulolytic system of Vol- 21 22 23 24 25 26 27 28 29 30 31 32 33 34 ... extracellular enzyme activities during growth and fruiting Arch Microbiol 114, 161–165 53 Ohga, S., Smith, M., Thurston, C.F & Wood, D.A (1999) Transcriptional regulation of laccase and cellulase...
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Báo cáo khoa học: Functional fine-mapping and molecular modeling of a conserved loop epitope of the measles virus hemagglutinin protein pdf
... human melanoma cell line (Mel-JuSo) Ó FEBS 2003 1518 M M Putz et al (Eur J Biochem 270) ¨ expressing the hemagglutinin protein supposedly in its native conformation Mel-JuSo-H and -wt cell lines ... 0.5%, sodium azide 0.05%) and plated in 96-well U-bottom plates at a concentration of · 106 cellsÆmL)1 Cells were incubated for 60 on ice in serum samples diluted in FACS medium, washed and stained ... cytometry histograms of crossreactivity with hemagglutinin protein on Mel-JuSo-H cells (open histogram) and Mel-JuSo-wt cells (grey histogram) of mouse sera induced against diphtheria toxoid (C),...
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Báo cáo khoa học: Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal b-N-acetylhexosaminidase pot
... recently been characterized and shown to represent a novel intracellular regulator that controls enzyme activity, dimerization and extracellular secretion [10] Previous experiments were performed ... b-N-acetylhexosaminidases are novel enzyme regulators that must be intracellularly processed to control activity, dimerization, and secretion into the extracellular environment Biochemistry 46, 2719–2734 ´ ´ ´ ... glycosylation status of PoHex using mass spectrometry The occupancy of individual sites of glycosylation clearly indicates that one site localized in the predicted propeptide and five classical sites in...
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Báo cáo khoa học: Sequences, geographic variations and molecular phylogeny of venom phospholipases and threefinger toxins of eastern India Bungarus fasciatus and kinetic analyses of its Pro31 phospholipases A2 docx
... The enzymes have a preference to interact with the zwitterionic micelles (diC16PC and Triton X-100) rather than the anionic micelles (diC16PC and deoxycholate) [3] However, substrate binding to ... ester is probably different from that of the phospholipid micelles The elapid venom 3FTx are a large multigene family and recent phylogenetic analyses of all the 3FTx revealed that kraits’ venom ... respectively [1,40,45] Notably, venom PLAs of different genera of elapids are clearly resolved with high bootstrap supports in the phylogenetic tree (Fig 7) The sea snakes have been shown [45,46] to be diphyletic...
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Báo cáo khoa học: Purification, characterization and molecular cloning of tyrosinase from the cephalopod mollusk, Illex argentinus docx
... Biosciences) A portion of the homogenate corresponding to 500 mg of ink sac was applied to an oligo(dT)-cellulose spun column Poly(A)+ RNA eluted from a column was ethanol-precipitated, and redissolved ... carried out with NCBI-BLAST 2.0 program available at DDBJ web server (http://www.ddbj.nig.ac.jp) Phylogenetic tree was deduced by a neighbor-joining analysis based on the alignment of amino acid sequences ... (IEF)-native 2DPAGE, ST94 was separated from other tyrosinase-active components and was observed as a clear spot corresponding to the position of a protein with pI 4.1 and a molecular mass of 94 kDa...
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Báo cáo khoa học: Cellular and molecular action of the mitogenic protein-deamidating toxin from Pasteurella multocida pptx
... responses in other cell types, such as cultured epithelial cells, including 4620 embryonic bovine lung cells [94], Vero cells [67,95,96], cardiomyocytes [70] and osteosarcoma cells [96] For example, ... osteoblasts [57,58,64] Cellular activity of PMT The intoxication of mammalian cells by PMT induces strong mitogenic [65–67] and anti-apoptotic [68–70] effects in various cell lines The cellular effects ... confluent Vero cells undergo rapid and dramatic morphological changes on toxin exposure [67,95,96] However, proliferating cell nuclear antigen and cyclins D3 and E are not upregulated in these cells on...
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Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii ppt
... recombinant cellobiohydrolase (A) SDS/ PAGE [10% (w/v) gel] analysis of purified recombinant cellobiohydrolase (reCBH) Lane 1, molecular makers; lane 2, uninduced cells; lane 3, induced cells (4 ... in the inclusion body fraction Cells were harvested by centrifugation (3630 g for min) from a 50 mL culture, and the cell pellet was resuspended in M urea The cell lysate was sonicated with three, ... cel7 expression Solka floc cellulose, lactose and beechwood xylan induce high levels of cel7 expression in T emersonii (Fig 4) Similar cellulase expression with complex cellulose has been documented...
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Báo cáo khoa học: Biochemical and molecular characterization of purified chicken pancreatic phospholipase A2 docx
... this enzyme have been identified in various tissues, and sPLA2-IB is reported to play a role in cell proliferation and hormone release via these receptors [6,7] Group IB sPLA2s have been isolated ... hydrolysis of phospholipids Bile salts dependence Several studies have provided evidence that micellar concentration of bile salts ensure the dispersion of the hydrolysis products [12,19] In order ... RKMIK 15 CAIPH CTIPG CTIPG CTIPP CKIPD CKIPS CV IPG This study [20] [14] [15] [13] [20] [21] DH5a cells Several clones were selected, and some of them contained a recombinant plasmid with a 500...
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Báo cáo sinh học: "Association between neuroserpin and molecular markers of brain damage in patients with acute ischemic stroke" pot
... damage, interleukin-6 (IL-6) and Intercellular Adhesion Molecule-1 (ICAM-1) as markers of inflammatory response, and matrix metalloproteinase (MMP-9) and cellular fibronectin (cFn) as markers ... Meager A: Cytokine regulation of cellular adhesion molecule expression in inflammation Cytokine Growth Factor Rev 1999, 10:27-39 29 Milner R, Campbell IL: The extracellular matrix and cytokines ... Pinteaux E: Adhesion to fibronectin regulates interleukin-1 beta expression in microglial cells Mol Cell Neurosci 2009, 41:148-155 32 Opdenakker G, Van den Steen PE, Van Damme J: Gelatinase b:...
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Báo cáo sinh học: " Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
... within pressed samples of fresh tissue Proprietary chemicals impregnated into the paper act to lyze cellular material and fix and preserve DNA and RNA within the fibre matrix [2] After a short drying ... field is becoming increasingly important as more developing countries initiate field trials of genetically modified crops A major goal of the DDPSC is to employ pathogen derived resistance strategies...
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báo cáo hóa học:" Association between neuroserpin and molecular markers of brain damage in patients with acute ischemic stroke" pdf
... damage, interleukin-6 (IL-6) and Intercellular Adhesion Molecule-1 (ICAM-1) as markers of inflammatory response, and matrix metalloproteinase (MMP-9) and cellular fibronectin (cFn) as markers ... Meager A: Cytokine regulation of cellular adhesion molecule expression in inflammation Cytokine Growth Factor Rev 1999, 10:27-39 29 Milner R, Campbell IL: The extracellular matrix and cytokines ... Pinteaux E: Adhesion to fibronectin regulates interleukin-1 beta expression in microglial cells Mol Cell Neurosci 2009, 41:148-155 32 Opdenakker G, Van den Steen PE, Van Damme J: Gelatinase b:...
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báo cáo hóa học:" Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
... within pressed samples of fresh tissue Proprietary chemicals impregnated into the paper act to lyze cellular material and fix and preserve DNA and RNA within the fibre matrix [2] After a short drying ... field is becoming increasingly important as more developing countries initiate field trials of genetically modified crops A major goal of the DDPSC is to employ pathogen derived resistance strategies...
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Báo cáo hóa học: " Error analysis and implementation considerations of decoding algorithms for time-encoding machine" ppt
... Advances in Signal Processing 2011, 2011:1 http://asp.eurasipjournals.com/content/2011/1/1 x (t ) Gm cell z (t ) Pulse Interval dt s g1 Page of to Voltage Converter Integrator g3 Hysteresis Quantizer ... distortion in the measurements An important linear component in the system is the amplifier (the Gm cell in Figure 1) When the amplifier is nonlinear, not only does it fail to amplify the signal as ... very large elements that amplify the noise in the measurements There may also be disastrous cancellation that brings computation error [7] Using a short window is one way to control the noise...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008