... types In the present study, we aimed to investigate the profile of protein expression of blast populations from peripheral bloodandbonemarrow aspirates using a proteomic approach with 2D-electrophoresis ... Materials and methods Sample preparation and solubilization Blast samples from bonemarrow aspirates and peripheral blood were isolated from six patients with Ficoll-centrifugation and washed ... bonemarrow (Table 2) On/off-phenomena of the identified spots were observed in four cases: Spot No 19 (annexin 6) was found in patient A in the sample of peripheral blood but not in bone marrow, ...
... in the bonemarrow (Figures and 6) At the end of our evaluation, the patient was diagnosed as suffering from the simultaneous presence of splenic MCL andbonemarrow MZL with peripheral blood ... form of indolent lymphoma originating in the spleen, characterized by prominent splenomegaly and variable involvement of lymph nodes, bone marrow, peripheral blood, and other organs Bonemarrow ... markers of MCL seemed to exclude a diagnosis of MCL in the bonemarrow In addition, the immunophenotype of circulating lymphocytes was similar to that ofbonemarrow neoplastic cells Page of The...
... on day 0, 4, 7, 11 and 14 to monitor the toxicity of CY on bonemarrow by measuring WBC number in the peripheral blood Twenty microliters ofblood was mixed with 380μl of Randolph’s solution ... were sacrificed on day 14 and the gastric and duodenal tissues were collected for biochemical and histological assessments Assessment of white blood cell (WBC) count Blood samples were collected ... Experimental animals and drug administration This study was conducted with the consent of the Committee on the Use of Live Animals in Teaching and Research of the University of Hong Kong Male ICR...
... makes reference to and is in coherence with the 2004/23/EC directive on donation, procurement and testing of human cells and tissues and with directive 2002/98/EC on human bloodandblood components ... inoculation of TSB and FTM media with 1% of the total volume of the product under validation as stated in European Pharmacopoeia (2.6.27) For the latter, 500 μl of whole bloodand 100 μl of the BM-MNC ... density gradient centrifugation (bone marrow selected cells) (n = 4) Phenotypical analysis of whole bonemarrow cells and after density gradient centrifugation (bone marrow selected cells) (n = 4)...
... knees On bone histology, areas ofbone affected by BMLs exhibit reversal lines indicative ofbone remodeling [10] Small areas ofbone necrosis are also present, as is scar The extensive bone remodeling ... Crutchfield M, Jannausch ML: The associations ofbone mineral density andbone turnover markers with osteoarthritis of the hand and knee in pre- and perimenopausal women Arthritis Rheum 1999, ... Heart Study Offspring cohort included surviving descendants (and spouses of descendants) of the original Framingham Heart Study cohort [25] As part of a study of the inheritance of OA, selected...
... rats 2.1 Isolation and Cultivation of Rat/Mouse Mesenchymal Cells from BoneMarrow To obtain bonemarrow cells, tibias and femurs were isolated, the epiphyses were removed, the bones were individually ... weekly and evaluated after 1, 7, 14 and 21 days of culture (B) The number of MSCs labeled after and 21 days of culture without trypsinization (C) Mitomycin-pretreated cells labeled with Feridex and ... and cultured for up days The number of cells labeled with Feridex was constant even after days of Feridex incorporation (E-H) Evaluation of proliferative capacity of MSCs exposed to Feridex and/ or...
... treatment of heart, liver and principally, blood related disorders, [1,4] This plasticity and the availability of adult bonemarrow stem cells have made them a very promising source for research and ... whether adult bonemarrow cells are able to aid in the reconstitution of thyroid parenchyma, we conducted a study based on the implantation of male derived BM cells into thyroid gland of female rats ... of transient elevation of TSH and with transplantation of male derived BM cells, it is possible to asses their effect on thyroid function, and if they are able, to participate in the repair of...
... Gershwin ME: Expansion ofbonemarrow IFN-alpha-producing dendritic cells in New Zealand Black (NZB) mice: high level expression of TLR9 and secretion of IFNalpha in NZB bonemarrow J Immunol 2004, ... Page of 12 Generation of DCs from BM culturesand analysis of control BMDCs Previous reports showed that the administration of FL to mouse BMCs generates large numbers of BMDCs in vivo and in ... levels of DC-SIGN and CD86 and lower CD40 than did those of normal controls *P < 0.05; **P < 0.01 Results are represented as mean ± SD of independent experiments of seven SLE patients and eight...
... design of the study and helped to draft the manuscript, BM conceived of the study, and participated in its design and coordination and helped to draft the manuscript All authors read and approved ... Representative panel of a control mouse lung (A and B) constitutively expressing GFP and wild-type mouse lung (C) injected with eGFP bone marrow- derived cells (BMDCs) Panels D, E, F and G are representative ... pneumonectomy model of lung growth in mice, Voswinckel et al [12] found no evidence of endothelial cell engraftment and O'Neill and coll [13] failed to show any engraftment of BMDCs in a model of hypoxia-mediated...
... dot labeling and tracking of human leukemic, bonemarrowand cord blood cells Leuk Res 2006 Plett PA, Frankovitz SM, Orschell-Traycoff CM: In vivo trafficking, cell cycle activity, and engraftment ... 18.1% of total murine marrow [24] The results were shown as percentages of total number of transfused donor cells Histological analysis and detection of donor cells Thin sections (5 µm) of lung ... circulating blood volume (7 ml/ 100 g body weight) Number of QD-labeled donor cells in BM = Fraction of donor cells in BM × total number of BMCs The number of BMCs harvested from femurs and tibias...
... shell of most bones and it accounts for 80% of the total bone mass Cortical bone is mostly found in the shaft portion of long bones and the outer shell found around the spongy bone at the end of ... medullary cavity of long bones and interstitial spaces of cancellous bones The metabolic functions ofbone are storage of minerals, growth factor and fat, acid-base balance, detoxification and as an ... cancellous bone does not Secondly, bone surrounds blood in compact bone while blood surrounds bone in the cancellous bone On a microscopic level, the structure ofbone can be distinguished into woven and...
... “application of the principles and methods of engineering and life sciences toward fundamental understanding of structure-function relationship in normal and pathological mammalian tissues and the ... Xiao-Hui and my baby OuYang Xin-yi for their understanding and love during the years of my Ph.D pursuit Table of Content Table of Content I Acknowledgement…………………………………………………………………i II Table of content………………………………………………………………… ... regeneration of tendon and nerve tissues BECON 2001 Repair Medicine: Growth Tissue and Organs, Jun 25-26, 2001, NIH, Maryland, USA Goh JCH, Ouyang HW, Mo XM, Teoh SH, Lee EH The efficacy ofbone marrow...
... method of measuring bone density The T-score relates bone density to young, healthy, race and gender-matched bone It is easily obtained and provides an objective quantitative number The T-score and ... model and the explanatory variables of Tscore and body mass index demonstrated that the femoral failure rate could be as high as 87% if a patient has a T-score of -2.4 and a body mass index of 43 ... T-score of 2.3 and a body mass index of 28 kg/m2, which was revised to a THA at two months post-operatively; the other case was in a female patient with a T-score of -2.1 and body mass index of 28...
... that of copy number gains There were 11 regions of loss found in more than 13% of the primary tumors and 16 regions of loss found in more than 13% ofblood (Tables and 4) Thirty other regions of ... in at least 30% of both primary tumors andblood (Table 5) Among these, seven regions of copy number gain were found in more than 50% of both primary tumors andblood A gain of 5p15.33 was evident ... (53%) of the primary tumors and 19/30 (63%) of the blood samples This region was found to contain LAMA5, RPS21, CABLES2 and C20orf151 Gain of 22q13.33 was detected in 50% of both primary tumors and...
... train length of 10, with a slice thickness of 3.0 mm, a 1.0 inter slice gap, excitation, a field of view of 13 cm, and a matrix of 256 × 192 pixels was also obtained [8] Assessment of BMLs BMLs ... GG and RO were all involved in the design and implementation of the study including data collection and measurement MD, AE, AF, YY and FC were involved in the analysis and interpretation of the ... English DR, Giles GG, Sullivan RO, FM C: Association ofbonemarrow lesions with knee structures and risk factors for bonemarrow lesions in the knees of clinically healthy, community-based adults Semin...
... signs, and urine output Laboratory data included routine hematology andblood chemistry Severity of illness was assessed using the Acute Physiology and Chronic Health Evaluation (APACHE) II and ... this study were ICU and hospital mortality We evaluated the association of both a discrete episode of hypoglycemia, the severity of hypoglycemia, and BG variability on ICU and hospital mortality ... analysis of more than 66,000 individual patient admissions to 24 ICUs across Australia and New Zealand to: describe the incidence ofand clinical factors associated with early hypoglycemia and BG...
... combination of different methods of quantitative and qualitative approach which are as follows: - Document; - Synthesize; - Analyze and contrast Questionnaires and audio tapes of native American andof ... American and Vietnamese cultures reacted to the same circumstances The database of this study consisted of elicited written questionnaires and audio-tapes of natural conversations METHODOLOGY OF THE ... TABLE OF CONTENTS DECLARATION ACKNOWLEDGEMENT ABSTRACT TABLE OF CONTENTS LIST OF TABLE AND FIGURES PART I: INTRODUCTION 1 Rationale of the study Research questions Aims and Objectives of the...
... TABLE OF CONTENTS LIST OF TABLE AND FIGURES PART I: INTRODUCTION 1 Rationale of the study Research questions Aims and Objectives of the study Scope of the study Methodology of the study Design of ... Tran, X D 2009 A series of reports on the findings of “US and Vietnamese cultures in contrast through everyday conversation” Journal Science of Hanoi National University of Education 27 Tylor, ... C and Heritage, J 1990 Conversation Analysis Annual Review of Anthropology, vol 19 (1) pp 283-307 11 Hall, E.T 1976 Beyond culture Anchor Books/A Division of Random House, Inc 12 Hutchby, I and...
... cell (MNGC), blood vessel (BV), lymphoid aggregates (LYM), bonemarrow (BM) Fig 6.6: Histology of new bone formation (a) and (b) bone consists of mature bone (pink) and immature bone (cartilage) ... than that of the diameter of the cells [10] One of the key components ofbone tissue engineering is the concept of ECM ECM not only provides the structural and functional aspects of bone, it also ... bone disorders such as osteoporosis, osteoarthritis (OA) and Paget’s disease are common in the elderly and often lead to fractures of the bone It is estimated that 40% of women over the age of...