growth postprandial excretion and tryptic activity of juvenile mullet mugil platanus gu ¨nther
Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
... bonds between NH of Thr5 and CO of Arg2, as well as NH of Arg2 and CO of Thr5 Structure of cyclic hexapeptides – cYT peptide The NMR data of the cYT peptide were indicative of its flexible nature ... percentage inhibition of peptide-treated cells and expressed as the mean of three independent experiments activity of 40% at 100 lM concentration Both linear and cyclic ER peptides (lER and cER) showed ... intensity of ROE cross-peaks, and were assigned as strong, medium and weak ˚ represent the family Based on ROE violation > 0.2 A and allowed values of /, w in the Ramachandran map, only one family of...
- 14
- 657
- 0
Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf
... specific 5¢ -and 3¢ primers selected on the basis of the published sequences for tomato b-fructofuranosidase (GenBank accession no D11350 and S70040) Primers for the short isoform of b-fructofuranosidase ... of 50 kDa would suggest that we had purified the truncated isoform Cloning of the cDNA of two isoforms of tomato b-fructofuranosidase and recombinant expression in E coli For protein expression ... reactivity of some sera to a 9- and a 15-kDa band We could show that the 9-kDa band in the tomato extracts reacts with a specific antibody against the LTP from cherry, Pru av and the 15-kDa band...
- 11
- 533
- 0
Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt
... presence of minute amounts of endotoxins The comparison of LPS and MfGl-II in the LAL assay shows, as expected, activity of LPS in the range of down to 10 pgỈmL)1, whereas MfGl-II has only activity ... respectively [23,30], and the bands at Fig Infrared spectrum in the spectral range 1800–900 cm)1 (A) and – in the range of the negatively charged phosphate band mas (PO2 ) 1300– )1 1190 cm (B) of hydrated ... water-binding capacity of lecithin headgroups [30] Finally, MfGl-II exhibits a main band at 1245 cm)1 and further weak bands at 1220 and 1260 cm)1 Thus, the phosphate groups of this glycolipid are...
- 9
- 665
- 1
Báo cáo khoa học: Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational design pdf
... head domain of up to 4.3 A, and of exposed parts of the Rossmann domain of up to ˚ 2.7 A, are observed For the rest of the homotetrameric assembly, only smaller backbone displacements of ˚ up to ... 42.3 mm)1Ỉcm)1) One unit of P2Ox activity was defined as the amount of enzyme necessary for oxidation of lmolỈmin)1 of ABTS (which equals the consumption of lmolỈmin)1 of O2) under assay conditions ... Data processing and evaluation were performed using the origin 7.5 software Crystallization, data collection and refinement of the E542K and L537G mutants Crystals of the E542K, L537G and E542K ⁄...
- 17
- 438
- 0
Báo cáo khoa học: Construction and biological activity of a full-length molecular clone of human Torque teno virus (TTV) genotype 6 pptx
... SalI double-digestions of the input linear construct yielded three restriction fragments of approximately 2100, 740 and 890 bp with the first enzyme pair, and of 2200, 630 and 890 bp with the latter ... when cloned as tandem repeats [29] and CAV requires either tandem-repeat cloning or excision of the viral genome from the plasmid before transfection [30] The rescue and replication of the viral ... cultures, the production of TTV is scanty and requires cell activation [18,19] Because TTV in vivo infects healthy individuals chronically, strict regulation of virus multiplicity and of cell damage is...
- 12
- 446
- 0
Báo cáo khoa học: Coiled–coil interactions modulate multimerization, mitochondrial binding and kinase activity of myotonic dystrophy protein kinase splice isoforms pptx
... protein kinase (DMPK) isoforms and truncation mutants Structural domain organization of DMPK splice isoforms A, C, E and F, and N-terminal and C-terminal truncation mutants of DMPK E used in this ... terminus and the coiled-coil domain [16,17] However, the consequences of multimerization and association with other proteins for in vivo activity regulation of DMPK are not clear Activation of Citron ... sequence and (b) the DMPK C terminus [15] In vitro study of one of the six major DMPK splice isoforms has revealed that a relationship must exist between kinase activity and the state of multimerization...
- 13
- 392
- 0
Báo cáo khoa học: nNOS inhibition, antimicrobial and anticancer activity of the amphibian skin peptide, citropin 1.1 and synthetic modifications pot
... anticancer and antibacterial activity of membrane active peptides, suggests that the anticancer activity is also due to penetration and disruption of the membranes of the cancer cells The selectivity of ... regular a-helix along its entire length (Fig 4A) Analysis of the angular order parameters (S, / and w) [66] of these structures indicated that, except for the N- and C-terminal residues (Gly1 and ... Replacement of the following residues with Ala show (a) little change in activity for Asp4 and Ser11, and (b) significant reduction in the activity for Phe3, Lys7, Lys8 and Leu16; replacement of the...
- 13
- 480
- 0
Báo cáo khoa học: Enhancement of intracellular concentration and biological activity of PNA after conjugation with a cell-penetrating synthetic model peptide docx
... P/ACE-SYSTEM MDQ software (Beckman Coulter, Fullerton, CA, USA), and were normalized to the area of the internal standard e-fluoresceinyl lysine in order to eliminate irregularities of injection and buffer ... influence of nociception on the beating rate of CM measured on day of culture without and with pretreatment with either 0.2 lM of I or II on day and after seeding The basal beating rate of the CM ... involvement of nonendocytic mechanisms (Fig 3) On the other hand, energy-dependent and -independent mechanisms contributed differently to the cellular uptake of the naked PNA and of the conjugate...
- 7
- 325
- 0
Báo cáo Y học: Synthesis, conformational analysis and biological activity of cyclic analogs of the octadecaneuropeptide ODN Design of a potent endozepine antagonist pot
... analysis of the calculated structures revealed a short distances between NH of Leu7 and the side chain carboxyl group of the Asp6 residue (< 0.28 nm), and NH of Leu7 residue and C¼O of Leu5 (< ... of a potent endozepine antagonist (Eur J Biochem 268) 6049 Fig RP-HPLC monitoring of N- and C-terminal deprotections and on-resin cyclization of the linear precursor of cyclo128 OP Aliquots of ... the receptor affinity and selectivity of peptide ligands [13,14,27] The Ala-scan of OP has revealed that the side chain of each residue is required for the full activity of the peptide [12] These...
- 13
- 632
- 0
Báo cáo khoa học: Physical properties and surface activity of surfactant-like membranes containing the cationic and hydrophobic peptide KL4 potx
... lateral organization of GUVs prepared from surfactant-like lipids in the absence and presence of Ca2+ The yield of individual GUVs was very low in the presence of KL4, and the GUVs formed displayed ... of the structural and functional properties of the native proteins and thus may offer a useful basis for the design of agents for therapeutic intervention [7] Studies of different fragments and ... (DSC) and fluorescence confocal microscopy of giant unilamellar vesicles (GUVs), (b) the effect of the lipid composition on KL4 structure, in the absence and presence of Ca2+, using CD and (c)...
- 13
- 329
- 0
Báo cáo khoa học: Expression, purification and catalytic activity of Lupinus luteus asparagine b-amidohydrolase and its Escherichia coli homolog potx
... activity of the LlA and EcAIII proteins With some of the substrates, no enzymatic activity could be detected, indicating lack or possibly very low level of the corresponding activity Enzyme activity ... wish to thank Prof Michal Dadlez, Jacek Oledzki, and Jacek Sikora (Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw) for their help and discussion of the mass spectrometry ... According to Parry and Clark [37], expression of the ybiK gene responded to the presence of cysteine and to defects in the cysB gene, and the ybiK knockout mutation impaired the use of glutathione...
- 12
- 407
- 0
Báo cáo khoa học: Residues near the N-terminus of protein B control autocatalytic proteolysis and the activity of soluble methane mono-oxygenase doc
... degradation offers a mechanism by which their activity, and hence the activity of the whole enzyme complex, could be controlled It also offers an explanation for the persistence of the regulatory ... characterization of the mechanism of cleavage, the roles of specific amino acids near to the N-terminus in catalytic activity, and the significance of the cleavage reaction in vivo MATERIALS AND METHODS ... using BamHI and EcoRI The plasmids for expression of the C-terminally 6-Histagged G13Q mutant of protein B and N-terminal truncations thereof were constructed by PCR amplification of the appropriate...
- 9
- 288
- 0
Báo cáo khoa học: Effects of a tryptophanyl substitution on the structure and antimicrobial activity of C-terminally truncated gaegurin 4 doc
... hemolytic Ó FEBS 2002 Structure activity relationships of GGN4 analogues (Eur J Biochem 269) 4369 Table Antimicrobial activity (a) and hemolytic activity (b) of GGN4 analogue peptides Percent hemolysis ... activity of the peptide However, a remarkable increase of the hemolytic activity was observed concomitantly Conformational preferences of the GGN4 analogues Figure summarizes the CD results of ... its N- and C-terminal Ó FEBS 2002 Structure activity relationships of GGN4 analogues (Eur J Biochem 269) 4371 Fig Solution structures of GGN4 analogues Backbone atoms (N, Ca, and C¢) of the finally...
- 8
- 447
- 0
Báo cáo Y học: A critical motif for oligomerization and chaperone activity of bacterial a-heat shock proteins pot
... region on chaperone activity of HspH and HspF Thermally induced aggregation of citrate synthase at 43 °C is depicted as a function of time in the presence of various amounts of a-Hsp proteins (A–D), ... chaperone activity of HspH and HspF Chaperone activity of purified a-Hsp proteins was determined by the CS aggregation assay as outlined in the legend of Fig (A–C) C-Terminally truncated variants of ... in detail, the contribution of their subregions to chaperone activity and oligomerization Tight coupling of chaperone activity and complex assembly B japonicum HspH and HspF oligomers contain...
- 9
- 467
- 0
Báo cáo sinh học: " Relationship between the loss of neutralizing antibody binding and fusion activity of the F protein of human respiratory syncytial virus" doc
... to better understand the mechanism of action of HRSV neutralizing antibodies and the impact of resistance to these antibodies upon the fusion activity of the F protein A summary of these results ... sitedirected mutagenesis of the HRSV F protein AD and RS participated in the design, oversight of the conduct of the experiments, and interpretation of the results All authors have read and approved the ... between subgroup A and B, as well as with bovine RSV Given this high degree of conservation, it was somewhat surprising that the functional analysis of the fusion activity of the panel of mutations...
- 4
- 271
- 0
báo cáo hóa học: " Kinematics and muscle activity of individuals with incomplete spinal cord injury during treadmill stepping with and without manual assistance" docx
... waveforms and kinematic profiles of control subjects with the profiles of each spinal cord injury subject with and without manual assistance [33-35] R= Σxi yi ( ) ( ) 1/ Σxi2 1/ Σyi2 , where xi and ... integrity of the data, and then rectified and low-pass filtered (25 Hz) Kinematic data were low pass filtered at Hz [32] Averaged EMG and kinematic profiles were time normalized to the percentage of ... two series of data, and i = 0, 1, 2, , N1 The first series of data was the averaged control subject Page of 14 (page number not for citation purposes) Journal of NeuroEngineering and Rehabilitation...
- 14
- 434
- 0
Báo cáo hóa học: " Relationship between the loss of neutralizing antibody binding and fusion activity of the F protein of human respiratory syncytial virus" doc
... to better understand the mechanism of action of HRSV neutralizing antibodies and the impact of resistance to these antibodies upon the fusion activity of the F protein A summary of these results ... sitedirected mutagenesis of the HRSV F protein AD and RS participated in the design, oversight of the conduct of the experiments, and interpretation of the results All authors have read and approved the ... between subgroup A and B, as well as with bovine RSV Given this high degree of conservation, it was somewhat surprising that the functional analysis of the fusion activity of the panel of mutations...
- 4
- 252
- 0
Báo cáo toán học: "Chemical composition and antibacterial activity of the essential oils from Launaea resedifolia L." docx
... composition and antibacterial activity of the essential oils from Launaea resedifolia L Amar Zellagui1, Noureddine Gherraf*1, Segni Ladjel2 and Samir Hameurlaine2 Laboratory of Biomolecules and Plant ... essential oils of L resedifolia There no reports on the essential oils of the species of the genus launaea except that reported by Cheriti et al [6] In continuation of our phytochemical and antibacterial ... phytochemical and antibacterial studies of the Algerian Sahara medicinal plants [7–11], we report here the findings of our studies on the composition and antimicrobial activity of L resedifolia essential...
- 13
- 455
- 0
báo cáo hóa học:" Chemical composition and antibacterial activity of the essential oils from Launaea resedifolia L." potx
... composition and antibacterial activity of the essential oils from Launaea resedifolia L Amar Zellagui1, Noureddine Gherraf*1, Segni Ladjel2 and Samir Hameurlaine2 Laboratory of Biomolecules and Plant ... essential oils of L resedifolia There no reports on the essential oils of the species of the genus launaea except that reported by Cheriti et al [6] In continuation of our phytochemical and antibacterial ... phytochemical and antibacterial studies of the Algerian Sahara medicinal plants [7–11], we report here the findings of our studies on the composition and antimicrobial activity of L resedifolia essential...
- 13
- 388
- 0
Báo cáo hóa học: " Chemical composition and antibacterial activity of the essential oils from Launaea resedifolia L" pdf
... S, Zellagui A (2010) The antibacterial activity of water extracts of Traganum nudatum Del (Chenopodiaceae) growing in Algeria Der Pharmacia Lettre 2(6):142–145 Zellagui et al Organic and Medicinal ... necessity and in this regard, plant essential oils may offer a great potential and hope Several studies have reported the efficacy of antibacterials obtained from the essential oils of various ... Pseudomonas aeruginosa, and Klebsielle pneumoniae In addition, the composition of volatile compounds was also determined 2.6 Antimicrobial activity The quantification of antibacterial activity for L resedifolia...
- 4
- 478
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25