glycosaminoglycan chain analysis and characterization glycosylation epimerization
A combined statistical and in silico framework for analysis and characterization of microbial and mammalian metabolic networks
... statistical analysis 60 4.3.4 In silico metabolic flux analysis 63 4.3.5 Sensitivity analysis of amino acid and glucose consumption 71 4.3.6 Analysis of the metabolite consumption and ... reconstruction and analysis of genome-scale mouse model The existing model was used as template and the network was expanded by compiling the information (genome, biochemical and mouse physiological ... perspective from which to consider, analyze, and ultimately understand the living world, whereas physics and chemistry come handy in probing the behavior of molecules and their activity inside living cells...
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Tài liệu Báo cáo khoa học: The resident endoplasmic reticulum protein, BAP31, associates with c-actin and myosin B heavy chain Analysis by capillary liquid chromatography microelectrospray tandem MS ppt
... BAP31–Flag and the endogenous BAP31 proteins, migrating at apparent masses of 33 kDa and 28 kDa, respectively, and two additional protein bands at apparent masses of 42 and 190 kDa [9] For sequence analysis, ... procaspases and 3, by the measurement of total DEVDase activity, and by the cleavage of the caspase targets poly(ADP-ribosyl) polymerase and endogenous BAP31 In contrast, cytoplasmic membrane blebbing and ... BAP31 and myosin, we show that BAP31 specifically associates with nonmuscle myosin B heavy chain and c-actin This suggests an additional role for BAP31 in the ER membrane architecture, traffic, and/ or...
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Tài liệu Báo cáo khoa học: Domain IV of mouse laminin b1 and b2 chains Structure, glycosaminoglycan modi®cation and immunochemical analysis of tissue contents pptx
... IV (A) from mouse laminin b1 (top) and b2 (bottom) chains and domain structure of the laminin b1 and b2 chains (B) (A) Both sequences show 41% identity (bars) and 29% conservative changes (dots) ... b1IV and b2IV assay and also by an assay speci®c for the laminin c1 chain [37] This demonstrated that 71±93% of the extractable laminin c1, b1 and b2 chain were already solubilized by EDTA and ... Laminin b1 and b2 chains have the same domain structures consisting of LN and LE (circles) modules, domain IV, and a coiled-coil (cc) domain II,I in the short arm of these laminin chains [2]...
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Báo cáo khoa học: Characterization of rat cathepsin E and mutants with changed active-site residues and lacking propeptides and N-glycosylation, expressed in human embryonic kidney 293T cells ppt
... N -glycosylation mutants and expressed them in HEK-293T cells to determine the importance of N -glycosylation in the processing, maturation, and trafficking of this protein Pulse–chase analysis and ... single (N92Q or N324D) and double N -glycosylation sites (N92Q ⁄ N324D) Asn residues in the two potential N -glycosylation sites (92NFT94 and 324NVT326) are substituted by Gln and ⁄ or Asp residues ... propeptides, and N -glycosylation of cathepsin E in its processing, maturation, and trafficking must be of particular importance In this study, to understand the molecular basis of the processing and intracellular...
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Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx
... re-testing and validating the effects on cell growth by transforming W303.1C and plating the cells at 23 °C, 30 °C and 37 °C A cold-sensitive growth phenotype (where toxin is active and interferes ... two N -glycosylation sites [19], although only one of these sites is usually used [21] The extent of N -glycosylation of Temperature-dependent ricin A chain mutants RTAD, RTAF108L ⁄ L151P and RTAE177D ... immunoprecipitated and either treated with (+) or without (–) Endoglycosidase H to determine the presence and extent of N-linked glycosylation As size controls, in vitro translations of mature RTA and Kar2SP-RTA...
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Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot
... chloroform and once with chloroform, and RNA was precipitated with 2-propanol and dissolved in RNase-free water Single-stranded RNAs were allowed to anneal by mixing equal amounts of each strand, heating ... procedures A DNA band at the expected size was amplified from the eyestalk and stomach, and another weak one from muscle (Fig 2B) In vivo CHH gene suppression using double-stranded RNA To investigate ... compilation ª 2006 FEBS J M Lugo et al Functional analysis of CHH by RNAi A B Fig CHH sequence analysis comparison (A) Sequence analysis comparison by CLUSTALW analysis among CHH cDNA reported for Marsupenaeus...
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báo cáo khoa học: " Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing" ppsx
... YY and DQ carried out the microarray analysis and small RNA sequencing, participated in the long npcRNA identification, and draft the manuscript MX and ZH carried out ChIP analysis, NS, CX and ... the two genotypes (Figure 10b), and negative correlation in expression levels was observed between sense strand and antisense strand expression patterns in both JD8 and JD8-Pm30 (Figure 10) In addition, ... out of plant, animal and microorganism kingdoms, and were wheat specific except for two SRP 7SRNA variants (TalnRNA9 and TalnRNA12) and U3 snoRNAs (TahlnRNA12 TahlnRNA23 and TahlnRNA29), which...
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Báo cáo y học: " Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1" docx
... analysis and drafted the manuscript XM and LC participated in its design and coordination and helped to draft the manuscript GH and ZR participated in its design and experimental work LZ participated ... functions of the ORFs As shown in Fig and 10, the secondary structure of gp17 protein was composed of 33.10% Alpha helix (Hh), 25.25% Extended strand (Ee) and 30.29% Random coil (Cc) The PHYRE program ... nodes helices and b strands with intermittent unstructured intervening regions The arrangement is characteristic of crystallized hydrolase Domain B is composed of a helix and b strands with intermittent...
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Analysis of the supply chain design and planning issues models and algorithms
... global supply chain focuses on the models and algorithms for global supply chain design and planning 2.1 Domestic Supply Chain In this thesis, the research of domestic supply chain design and planning ... exchange rate and price, many other random features such as uncertain demand (Sodhi, 2005) and political risk (Nagurney and Matsypura, 2005) have been explored in global supply chain design and planning ... chain and global supply chain The former one refers to supply chain design and planning without consideration of international characteristics such as currency exchange rates, import duties and...
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Design, analysis and application of divisible load scheduling strategies in linear daisy chain networks with system constraints
... wi , and is defined as wi = Time taken by Pi to process a unit load Time taken by a standard processor to process a unit load (2.1) The speed of Pi is inversely proportional to wi and the standard ... procedure, we found that t1,n remains the same and only P1 and P2 satisfy (3.35), and hence the result The finish time is given by, 1.83 × 103 unit and Fig 3.5 illustrates the final solution Heuristic ... simulation experiments as EXA1, EXB1, EXA2, and EXB2 Note that while A and B denote the strategies discussed in Section 3.3, the numbers and in EXA1, EXB1, EXA2, and EXB2, denote two distinct possible...
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Development and characterization of refractive solid immersion lens technology for far field integrated circuit faillure analysis using laser induced techniques
... Analysis (FA) is a critical element and basically involves performing a series of electrical and physical inspections to analyze and understand failures down to their root cause This understanding ... plate and Fresnel Phase plate DSIL fabrication by Gallium implantation and differential etching Standard SNOM setup consisting of a) an illumination unit, (b) collection and redistribution unit and ... meridional plane for a probe depth of μm and for numerical apertures of (a) 0.3, (b) 0.6, and (c) 0.9 The calculations are for air (n1 = 1.0) and glass (n2 = 1.5) and a wavelength of λ = 0.6328 μm (He–Ne...
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N glycosylation analysis and comparative modeling of mouse hybridoma lgm84 85
... heavy and light chains Fab is the region where domains VL, CL, VH and CH1 associate; Fc is the region where domains CH2 and CH3 from each heavy chain associate; and Fv is the region of VL and VH and ... and respectively In mammals, there are two types of light chains across all Ig isotypes i.e and One Ig monomer consists of four polypeptide chains; two heavy chains (H) and two light chains ... of heavy and light chains of IgM 84 and 85, the sequence alignment results of the corresponding constant and variable regions, and the respective potential N -glycosylation sites on each chain Appendix...
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SAP2000® Linear and Nonlinear Static and Dynamic Analysis and Design of Three-Dimensional Structures
... used in the analysis The message in Figure 37 indicates that the analysis and design sections are different The goal is to repeat the analysis and design process until the analysis and design ... Run Analysis command or the Run Analysis button, and then click the Run Now button on the Set Analysis Cases to Run form F When the analysis is complete, click the OK button to close the analysis ... both Load Cases and Analysis Cases exist, and they may be different However, the program automatically creates a corresponding analysis case when a load case is defined, and the analysis cases...
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SAP2000 Integrated Finite Elements Analysis and Design of Structures
... EFFORT AND EXPENSE HAVE GONE INTO THE DEVELOPMENT AND DOCUMENTATION OF SAP2000 THE PROGRAM HAS BEEN THOROUGHLY TESTED AND USED IN USING THE PROGRAM, HOWEVER, THE USER ACCEPTS AND UNDERSTANDS THAT ... time, but want to see the results of the pushover analysis, then you can read parts A and B, skip part C and the first five steps of part D, and then open the already created model (with the ... then run the linear static and dynamic analysis by clicking Run on the Analyze menu Next select Run Static Pushover from the Analysis menu to perform the pushover analysis The results are now...
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Analysis and compensation of log_domain filter deviations due to transistor nonidealities
... parasitic emitter and base resistances, finite beta, Early effect, and area mismatches SPICE simulations, both large and small-signal andysis, are perforrned to verify the results By understanding the ... emitter and base resistances, finite beta, Early effect, and area mismatches The results are used to predict the nonideal lowpass and bandpass biquadratic filter responses Through Our understanding ... filters with ideal and realistic transistor models: (a) lowpass biquad filter, (b) bandpass biquad filter, (c) 7th-order Chebyshev lowpass ladder filter, and (d) 6th-order Chebyshev bandpass filter...
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Rate-Distortion Analysis and Traffic Modelling of Scalable Video Coders
... coded with various standards and propose a traffic model for multi-layer VBR video traffic A good traffic model is important to the analysis and characterization of network traffic and network performance ... tractable, and with low computational complexity • Give a detailed R-D analysis and propose novel R-D models for scalable video coders To better understand scalable coders, we examine distortion and ... video standards are frame-based and block motion-compensated DCT coding Furthermore, standards only specify bitstream syntax and decoding semantics, which leaves the implementation of encoder and...
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Security analysis and business valuation on wall street
... Security Analysis No Profit Guarantee Day-to-Day Trading and Security Analysis Herd Psychology and Security Analysis Momentum Investors Game Theory and Security Analysis The Premise of Security Analysis ... Australia, and Asia, Wiley is globally committed to developing and marketing print and electronic products and services for our customers’ professional and personal knowledge and understanding The ... data services Starting the analysis, industry analysis, and company-specific analysis Chapters 5, 6, and have been revised and updated The principal themes remain the same, and the chapters are more...
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DATA ANALYSIS AND DISCUSSION
... to convey my deepest gratitude to my husband, my children, and my parents for their constant support and thorough understanding.Their great encouragement and love have helped me to overcome the ... method, instruments as well as process of data collection and data analysis IV Data analysis and discussion This focuses on presenting, analyzing and discussing the results obtained from the study V ... only meets the demands of communication but also supports students’ needs as well 2.2 The role of attitudes in language learning Attitudes and learning go hand in hand Garder and Lamber (1972)...
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Critical thinking skills developing effective analysis and argument
... similarities and differences, absence and presence, order and sequence; using recognition of pattern in order to compare and contrast items and to predict possible outcomes; sorting and labelling ... other, and the use of interim concIusions Understanding the structure of an argument is beneficial both in making reading faster and more effective, and in structuring your own arguments Chapters and ... attended family parties and funerals Having had her own band for several years, she knows the life of a rock band from the inside However, a she was never s a member of this band and was not in competition...
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