gene symbol immt isoform 3 of mitochondrial inner membrane protein
Expression dynamics of the hepatic mitochondrial proteome of the sod2+ mouse in response to troglitazone administration
... 136 4.1.2 Purity of mitochondria preparation 136 4.1 .3 The HET liver mitoproteome 137 4.1 .3. 1 Redox proteins 140 v 4.1 .3. 2 OXPHOS 141 4.1 .3. 3 Urea ... PPAR-agonist targets 1 23 3 .3. 3 .3 Parallel proteome shift suggests ROS-induced mitochondrial stress 126 3. 3.4 Prolonged troglitazone treatment activates FOXO3a through oxidative stress-mediated ... 101 3. 3.1 2D-DIGE Analysis of Troglitazone-induced HET Mitoproteome 101 3. 3.2 Analysis of different ACO2 fates under different oxidative stress conditions 104 3. 3 .3 8-plex...
Ngày tải lên: 12/09/2015, 11:08
Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt
... 39 8 37 6 39 9 415 415 36 9 37 7 4 13 4 23 427 938 755 I Loop region J wt B sku L372 L372 G374 G376 I369 I369 G374 V376 T3 83 T3 83 Y377 L 236 Y377 D 234 D 234 L 236 N379 N379 Fig Sequence alignment and predicted ... chain of the mutated residue (Val376) points toward the inside of the helix–loop–helix structure Consequently, the side-chain atoms of Val376 overlap with those of Ile369, Leu372 and Thr3 83 with ... Journal 277 (2010) 4452–44 63 ª 2010 The Authors Journal compilation ª 2010 FEBS K Urano et al 32 33 34 35 36 during the molting process Insect Biochem Mol Biol 35 , 1112–11 23 Hall CL (1978) Acyl-CoA...
Ngày tải lên: 18/02/2014, 04:20
Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx
... 3. 69 3. 23 4. 03 3.68 0 .30 5 24.4 · 1 03 23. 8–24.9 · 1 03 24.6 · 1 03 621 19 K364R Direct linear Confidence limits (68%) Wilkinson Standard error 5 .38 5.09–5.87 5.67 0.186 25.5 · 1 03 25.4–25.6 · 1 03 ... MCAD catalysis (R256T) and folding (K364R) mutants 29 30 31 32 33 34 35 36 37 38 acyl-CoA dehydrogenase causes sudden neonatal death J Clin Invest 94, 1477–14 83 Andresen BS, Jensen TG, Bross P, ... Km value of wild-type MCAD for octanoyl-CoA, 3. 69 lm, compares well with the published value of 3. 4 lm [ 13, 14] Although the Km is increased somewhat for the K364R mutant protein (5. 53 lm), the...
Ngày tải lên: 20/02/2014, 02:21
Báo cáo khoa học: The Y42H mutation in medium-chain acyl-CoA dehydrogenase, which is prevalent in babies identified by MS/MS-based newborn screening, is temperature sensitiv pptx
... Oxidation % of Controls Fig Western blot analysis of steady-state amounts of MCAD protein in lymphoblasts with genotypes K304E/K304E, K304E/Y42H, K304E/WT and WT/WT cultured at 34 °C, 37 °C and 39 °C ... loading control 100 80 K304E/K304E 60 K304E/Y42H K304E/WT 40 20 34 °C 37 °C 39 °C Temperature Fig Myristate oxidation from fibroblasts with genotypes K304E/ K304E, K304E/Y42H and K304E/WT as compared ... & Gregersen, N (19 93) Co-overexpression of bacterial GroESL chaperonins partly 23 24 25 26 27 28 29 30 31 32 33 34 35 overcomes non-productive folding and tetramer assembly of E coli-expressed...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: The gene carD encodes the aldehyde dehydrogenase responsible for neurosporaxanthin biosynthesis in Fusarium fujikuroi potx
... T3 and T4 were chosen for detailed phenotypic characterization Phenotype of DcarD mutants Comparison of T3 and T4 colonies with those of the preceding SF 134 strain confirmed a different color of ... version of this apocarotenoid 31 72 Experimental procedures Strains and growth conditions FKMC1995 [33 ] is a wild-type strain of F fujikuroi (formerly, G fujikuroi mating population C [34 ]) SF 134 , ... pH 7.5, lL of 100 mM NAD+), and 80 lL of crude protein and water were added to give a total volume of 200 lL Incubations were performed at 28 °C for 30 min, stopped by addition of mL of acetone,...
Ngày tải lên: 14/03/2014, 22:20
Báo cáo khoa học: Fasting-induced oxidative stress in very long chain acyl-CoA dehydrogenase-deficient mice pdf
... value of 32 .82 ± 2.0 nmolÆmg)1 that decreased to 22.58 ± 1.2 nmolÆmg)1 in WT mice, and a GSH value of 30 . 53 ± 1.5 nmolÆmg)1 that decreased to 21.02 ± 1 .3 nmolÆmg)1 in VLCAD KO mice RT-PCR and gene ... secretion of VLDL Overall, there is increasing evidence that an inherited enzyme defect in mitochondrial b-oxidation also affects many other pathways of lipid metabolism [ 13] The transcription of genes ... (20 03) MS ⁄ MS-based 4706 newborn and family screening detects asymptomatic patients with very-long-chain acyl-CoA dehydrogenase deficiency J Pediatr 1 43, 33 5 34 2 Kompare M & Rizzo WB (2008) Mitochondrial...
Ngày tải lên: 15/03/2014, 23:20
Báo cáo Y học: Interaction of the GTS1 gene product with glyceraldehyde3-phosphate dehydrogenase 1 required for the maintenance of the metabolic oscillations of the yeast Saccharomyces cerevisiae pdf
... Tamura, K & Tsurugi, K (1999) Protein interactions of Gts1p of Saccharomyces cerevisiae 33 34 35 36 37 38 39 40 41 42 43 throughout a region similar to a cytoplasmic portion of some ATP-binding cassette ... pACGTS1[C53Y]/gts1D tdh1D tdh2D tdh3D pACTDH1/tdh1D pACTDH1pr.TDH2/tdh1D pACTDH1pr.TDH3/tdh1D 5. 03 5.12 5.10 5.08 5. 03 5.01 4.58 3. 32 4.95 3. 40 2.45 ± ± ± ± ± ± ± ± ± ± ± 0.08 0.11 0.18 0. 23 0.15 0. 13 ... that of GTPase-activating proteins of ADP-ribosylation factors [28 ,30 ,31 ] and the dimerization domain is similar to the sequence downstream of Walker’s motif A of some ABC transporters [32 ,38 ]...
Ngày tải lên: 31/03/2014, 21:21
Phân lập Promoter của Gene mã hóa cho enzyme cinamyl alcohol dehydrogenase
... 1.28 1 .33 1.22 1 .33 14 Linum_album 0 .39 0 .36 0 .33 0 .39 0.41 0.87 0 .31 0.41 0.27 0.29 0 .30 0.28 1.27 15 Eucalyptus_gunnii 0 .37 0 .38 0 .36 0 .35 0 .36 0.81 0 .32 0 .38 0.25 0.25 0 .30 0.27 1.41 0 .31 16 ... 1 .34 1 .30 1. 23 1. 23 1. 23 1. 63 1.28 1 .37 1.27 1 .34 1.20 1 .35 0. 03 1.27 1.40 0.01 0.01 19 Eucalyptus_cordata 1 .33 1.29 1. 23 1. 23 1. 23 1.60 1.27 1 .38 1.27 1 .32 1.20 1 .31 0.01 1.26 1.40 0. 03 0. 03 ... 1.67 1 .31 1 .36 1.27 1 .34 1. 23 1 .32 0. 03 1.28 1.40 0.02 0. 03 0.02 0. 03 0.02 1 .35 0. 03 24 Eucalyptus_punctata 1 .32 1.26 1.22 1.21 1.21 1.57 1.26 1 .35 1.24 1 .32 1.20 1 .30 0. 03 1.25 1 .38 0. 03 0. 03 0.02...
Ngày tải lên: 09/11/2012, 16:13
Phân lập Promoter của Gen mã hóa cho enzyme cinnamyl alcohol Dehydrogenase (Cad) và thiết kế vector chuyển Gen mang đoạn gen mã hóa cho Enzyme cinnamoyl CoA reductase (CCR) từ cây bạch đàn Uro
... 1.28 1 .33 1.22 1 .33 14 Linum_album 0 .39 0 .36 0 .33 0 .39 0.41 0.87 0 .31 0.41 0.27 0.29 0 .30 0.28 1.27 15 Eucalyptus_gunnii 0 .37 0 .38 0 .36 0 .35 0 .36 0.81 0 .32 0 .38 0.25 0.25 0 .30 0.27 1.41 0 .31 16 ... 1 .34 1 .30 1. 23 1. 23 1. 23 1. 63 1.28 1 .37 1.27 1 .34 1.20 1 .35 0. 03 1.27 1.40 0.01 0.01 19 Eucalyptus_cordata 1 .33 1.29 1. 23 1. 23 1. 23 1.60 1.27 1 .38 1.27 1 .32 1.20 1 .31 0.01 1.26 1.40 0. 03 0. 03 ... 1.67 1 .31 1 .36 1.27 1 .34 1. 23 1 .32 0. 03 1.28 1.40 0.02 0. 03 0.02 0. 03 0.02 1 .35 0. 03 24 Eucalyptus_punctata 1 .32 1.26 1.22 1.21 1.21 1.57 1.26 1 .35 1.24 1 .32 1.20 1 .30 0. 03 1.25 1 .38 0. 03 0. 03 0.02...
Ngày tải lên: 12/11/2012, 18:01
Phân lập promoter của gen mã hóa cho enzyme cinnamyl alcohol dehydrogenase và thiết kế vector chuyển gen mang đoạn gen mã hóa cho enzyme cinnamoyl CoA reductase từ cây bạch đàn uro
... 1.28 1 .33 1.22 1 .33 14 Linum_album 0 .39 0 .36 0 .33 0 .39 0.41 0.87 0 .31 0.41 0.27 0.29 0 .30 0.28 1.27 15 Eucalyptus_gunnii 0 .37 0 .38 0 .36 0 .35 0 .36 0.81 0 .32 0 .38 0.25 0.25 0 .30 0.27 1.41 0 .31 16 ... 1 .34 1 .30 1. 23 1. 23 1. 23 1. 63 1.28 1 .37 1.27 1 .34 1.20 1 .35 0. 03 1.27 1.40 0.01 0.01 19 Eucalyptus_cordata 1 .33 1.29 1. 23 1. 23 1. 23 1.60 1.27 1 .38 1.27 1 .32 1.20 1 .31 0.01 1.26 1.40 0. 03 0. 03 ... 1.67 1 .31 1 .36 1.27 1 .34 1. 23 1 .32 0. 03 1.28 1.40 0.02 0. 03 0.02 0. 03 0.02 1 .35 0. 03 24 Eucalyptus_punctata 1 .32 1.26 1.22 1.21 1.21 1.57 1.26 1 .35 1.24 1 .32 1.20 1 .30 0. 03 1.25 1 .38 0. 03 0. 03 0.02...
Ngày tải lên: 03/04/2013, 10:00
Tài liệu Báo cáo khoa học: Metabolic gene switching in the murine female heart parallels enhanced mitochondrial respiratory function in response to oxidative stress pdf
... 1.6 9.6 2.0 0.1 28 .3 A 32 .6 165.8 30 .7 2.6 436 .0 db ⁄ + ± ± ± ± ± 1.9 9.8 2.4 0.1 32 .3 36.6 175.6 38 .8 2.2 38 1.0 db ⁄ db ± ± ± ± ± 1.7* 7.1 1. 03 0.04 20.4 35 .0 177.8 36 .8 2 .3 411.6 ± ± ± ± ± 2.0 ... (nmolÆmin Æmg protein) State respiration (nmolÆmin)1Æmg)1 protein) State respiration (nmolÆmin)1Æmg)1 protein) ADP ⁄ O Phosphorylation rate (nmolÆmin)1Æmg)1 protein) 29.4 145.0 33 .5 2.5 36 3.4 db ⁄ ... L & Wieckowski MR (1999) The mechanisms of fatty acid-induced proton permeability of the inner mitochondrial membrane J Bioenerg Biomembr 31 , 447–455 13 Schreiber SN, Emter R, Hock MB, Knutti...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Metabolic control of mitochondrial properties by adenine nucleotide translocator determines palmitoyl-CoA effects Implications for a mechanism linking obesity and type 2 diabetes pdf
... 1.280. 03 ) 0. 030 .01 0. 130 . 03 0.010.01 ) 0.100.04 ) 0.750.08 0.260.04 ) 0.490.04 ) 0.140.04 0.150. 03 ) 0.070.10 ) 0. 830 .29 ) 3. 320. 43 ) 0.180. 03 0.160.05 0.010.05 0.000.00 0. 030 .11 ) 0.710. 13 ) 0.020.00 ... 0.590.21 ) 0.200.08 1 .30 0.40 ) 1.280 .36 * ) 0.880.25 0.410.06 0. 230 .04 ) 0.040.01 0.480.06 0. 230 .02 ) 1 .31 0. 03 0.280. 03* 0 .30 0.04 ) 0.110.02** 0. 530 .07 0.480. 03* * ) 1.470.09 *P < 0.05 and **P < ... 0.020.00 ) 0. 530 .08 0 .33 0.08 ) 0.100. 13 0.000.00 ) 0.010. 03 0.180.06 0.000.00 0.400.10 0.020.01 0.000.00 0.000.00 0.000.01 0.060.01 0.000.00 0.080.01 0 .33 0.11 ) 0.010. 03 ) 0.010.00 0.090.27 3. 791.44...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx
... of the lipid acyl hydrolase activity of the major potato (Solanum tuberosum) tuber protein, patatin, by cloning 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 and abundant expression in a baculovirus ... compartment (expression of the 63 kDa isoform which does not possess a mitochondrial localization signal) or into the mitochondrial compartment (by production of isoforms containing a mitochondrial localization ... with excitation wavelengths of 488 and 633 nm Visualization was performed with the following wavelengths: (1) 488 nm, (2) 633 nm, (3) 488 and 633 nm The colocalization of iPLA2c-GFP and DsRED2-mito...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: Diverging regulation of pyruvate dehydrogenase kinase isoform gene expression in cultured human muscle cells pot
... phosphatase (PDP) To date, four isoforms of PDK (PDK1–4) and two isoforms of PDP (PDP1–2) have been identified in humans [3 5] These isoforms display unique tissue distribution [3 5] and varied kinetic ... Molecular cloning of each PDK or PDP isoform was confirmed by sequence comparison of each clone with 30 05 Regulation of hPDK2 and hPDK4 gene expression the previously reported DNA sequences [3 5] This ... Downstream targets of PtdIns3K include glycogen synthase kinase -3 (GSK3) and the mammalian target of rapamycin (mTOR) GSK3 is inactivated in response to insulin via a PtdIns3K ⁄ protein kinase B...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo khoa học: Glycation damage targets glutamate dehydrogenase in the rat liver mitochondrial matrix during aging pptx
... 124– 136 125– 137 152–162 1 63 171 172–1 83 212– 231 2 13 231 481–496 504–516 524– 535 527– 535 536 –545 1016.45 1581. 73 1425. 63 1241.59 10 13. 53 1219.61 2060.01 1 931 .9 17 23. 88 1507.72 1410.74 9 63. 53 1079.57 ... DM z Xcorr 38 –46 221– 232 252–262 36 5 37 9 431 –4 43 468–475 988.52 133 7.65 1276.62 18 03. 92 14 93. 70 990.54 0 .33 0 .37 0 .38 0. 53 0.44 0.22 2 2 2 2. 03 2.52 2.65 2.07 4 .38 2.44 10 32 Sequence Position ... K.DAQLFIQR.K Protein UniProt accession No Sequence Spot No 39 –46 71–80 130 –141 142–1 53 211–221 222– 231 244–255 278–289 30 7 32 0 32 1 33 0 915.56 1117. 63 1278.67 139 2.77 1216.61 1 131 .57 133 3.64 1429.71...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Malonyl-CoA decarboxylase (MCD) is differentially regulated in subcellular compartments by 5¢AMP-activated protein kinase (AMPK) Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique potx
... J., Antinozzi, P.A., McGarry, J.D & Newgard, C.B (2001) Overexpression of a 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 modified human malonyl-CoA decarboxylase blocks the glucoseinduced increase ... the long isoform, which contributes to most of the increases in cytoplasmic MCD protein, may be less active than the short form Our data suggest that AMPK augments levels of both isoforms of MCD ... blots are representative of n ¼ experiments (B) MCD expression (i), optical density of 54.7 kDa isoform (ii), optical density of 50.7 kDa isoform (iii) and activity (iv) of cytoplasmic fractions...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: 17b-Hydroxysteroid dehydrogenase type 11 is a major peroxisome proliferator-activated receptor a-regulated gene in mouse intestine pdf
... 766.47 898.47 936 .51 1000.58 1692.87 2527.58 2577.24 2 630 . 43 2951.48 2984.5 )0.02 0. 03 0.01 0.02 0. 03 0.02 0.02 0.01 )0. 03 0.04 2 83 288 289–296 151–161 63 70 140–1 53 3–24 83 105 33 –58 162–189 228–254 ... 620 .34 620 .34 745.46 8 73. 52 925. 23 970.62 1001.61 1117.62 1229.64 131 5.68 135 2. 63 1597.97 1728.97 1985.94 1686.94 2820.4 2969.42 0 )0.05 0.02 0 0. 03 0. 03 0.05 0.07 0.01 )0.05 0.05 0.05 0.07 )0. 03 ... disruption of the alpha isoform of the peroxisome proliferator-activated receptor gene in mice results in abolishment of the pleiotropic effects of peroxisome proliferators Mol Cell Biol 15, 30 12 30 22...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Silencing the expression of mitochondrial acyl-CoA thioesterase I and acyl-CoA synthetase 4 inhibits hormone-induced steroidogenesis potx
... a 43- kDa phosphoprotein by its capacity to increase mitochondrial steroidogenesis in a cell-free assay [15] The protein was then purified to homogeneity [15] Further cloning and sequencing of ... in MTE-I-deficient cells StAR protein was detected as a 30 -kDa protein after h of 8Br-cAMP treatment in mock-transfected cells (Fig 3A) The effect of 8BrcAMP on StAR protein concentrations was strongly ... regulates StAR protein at transcriptional and protein levels Effect of MTE-I overexpression on steroidogenesis To further explore the role of MTE-I in the regulation of steroidogenesis, we examined...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo Y học: Propionate CoA-transferase from Clostridium propionicum Cloning of the gene and identi®cation of glutamate 324 at the active site pdf
... Endoproteinase-AspN Trypsin 32 2TAESGAIGGVPAGGVRF 338 1547 (1547) Da 2718 (2717) Da 5107 (5105) Da 15 63 (1562) Da 2 733 (2 732 ) Da 5122 (5120) Da 1 533 (1 533 ) Da 2705 (27 03) Da 5094 (5091) Da 31 7DFMTLTAESGAIGGVPAGGVRFGASYNA344 ... Q-Sepharose Resource-Phe Resource-Q 32 00 1560 1428 130 6 139 1 46 20 15 2 .3 34 70 85 15 30 37 100 49 45 41 previously shown that glutamate thiol ester-containing proteins, such as CoA-transferases ... outer layers of a helices followed by one layer of b sheets and an inner layer of a helices This arrangement is found in both subunits of the protein It is remarkable that the outer layer of a helices...
Ngày tải lên: 17/03/2014, 17:20
Báo cáo khoa học: Human retinol dehydrogenase 13 (RDH13) is a mitochondrial short-chain dehydrogenase⁄reductase with a retinaldehyde reductase activity pdf
... membrane and some proteins of the intermembrane space and the inner membrane are devoid of such signals [20] The association of RDH 13 with the outer side of the inner mitochondrial membrane suggests ... that the mitochondrial targeting sequence is cleaved by matrix proteases on transfer of the protein across the inner mitochondrial membrane, and that all proteins of the mitochondrial outer membrane ... that RDH 13 is localized on the outer side of the inner mitochondrial membrane, facing the intermembrane space This submitochondrial localization of RDH 13 is consistent with the lack of a cleavable...
Ngày tải lên: 23/03/2014, 07:20