gene symbol dbt lipoamide acyltransferase component of branched chain alpha keto acid dehydrogenase complex mitochondrial
Expression dynamics of the hepatic mitochondrial proteome of the sod2+ mouse in response to troglitazone administration
Ngày tải lên :
12/09/2015, 11:08
... Representative proteome map of mouse liver mitochondria 81 Figure 17.Tandem mass spectrum of enoyl-CoA hydratase generated from MALDITOF/TOF MS/MS 82 Figure 18 Comparison of SOD1, SOD2, ... data of best scoring MS/MS of 3-hydroxyisobutyrate dehydrogenase 179 xii Figure 45 Supplemental data of best scoring MS/MS of enoyl-CoA hydratase 180 Figure 46 Supplemental data of ... al., 1997) and it is generally accepted that the main sites of ROS production are Complex I and Complex III (Orrenius et al., 2007) In addition, Complex II which serves Complex I and III has...
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Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt
Ngày tải lên :
18/02/2014, 04:20
... (short -chain acyl-CoA dehydrogenase, NP_000008); MCAD (medium -chain acyl-CoA dehydrogenase, NP_000007); LCAD (long -chain acyl-CoA dehydrogenase, NP_001599); VLCAD (very long -chain acyl-CoA dehydrogenase, ... isovaleric acidaemia not exhibit such accumulation of amino acids [2] because an enzyme reaction one step before IVD dehydrogenation, in which a-ketoisocaproate is catalysed by branched- chain a -keto acid ... These genetic and biochemical data, along with previous observations that the sku mutant accumulates isovaleric acid and branchedchain amino acids, strongly indicate that a single amino acid substitution...
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Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx
Ngày tải lên :
20/02/2014, 02:21
... 11 of the medium -chain acyl-CoA dehydrogenase gene Am J Hum Genet 54, 975–988 Sarkar G & Sommer SS (1990) The ‘megaprimer’ method of site-directed mutagenesis Biotechniques 8, 404–407 Goloubinoff ... and characterization of short -chain, mediumchain, and long -chain acyl-CoA dehydrogenases from rat liver mitochondria: isolation of the holo- and apoenzymes and conversion of the apoenzyme to the ... Macheroux P & Ghisla S (2004) Thermal unfolding of medium -chain acyl-CoA dehydrogenase and iso(3)valeryl-CoA dehydrogenase: study of the effect of genetic defects on enzyme stability Biochim Biophys...
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Báo cáo khoa học: The Y42H mutation in medium-chain acyl-CoA dehydrogenase, which is prevalent in babies identified by MS/MS-based newborn screening, is temperature sensitiv pptx
Ngày tải lên :
07/03/2014, 16:20
... the medium -chain acyl-CoA dehydrogenase (MCAD) gene changes a conserved arginine, previously shown to be functionally essential in short -chain acylCoA dehydrogenase (SCAD) Am J Hum Genet 53, 730–739 ... (1994) Intramolecular folding and assembly of medium -chain acyl-CoA dehydrogenase (MCAD) Demonstration of impaired transfer of K304E-variant MCAD from its complex with hsp60 to the native tetramer ... I & Ghisla, S (1996) Mediumlong -chain chimeric human Acyl-CoA dehydrogenase: mediumchain enzyme with the active centre base arrangement of long -chain Acyl-CoA dehydrogenase Biochemistry 35, 12402–...
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Báo cáo khoa học: The gene carD encodes the aldehyde dehydrogenase responsible for neurosporaxanthin biosynthesis in Fusarium fujikuroi potx
Ngày tải lên :
14/03/2014, 22:20
... have ectopically inte- Fig Generation of targeted DcarD mutants in a carS background (A) Schematic representation of the gene replacement event leading to the generation of hygromycin-resistant ... predicted to be the oxidation of the aldehyde group of b-apo-4¢-carotenal On the basis of sequence homology to ylo-1, we identified the carD gene and demonstrated, with genetic and biochemical approaches, ... Biochemical assays of CarD activity (A) Upper panel: HPLC analysis of in vitro assays of crude lysate of CarD-expressing cells incubated with b-apo-4¢-carotenal (peak a) The generated product...
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Báo cáo khoa học: Fasting-induced oxidative stress in very long chain acyl-CoA dehydrogenase-deficient mice pdf
Ngày tải lên :
15/03/2014, 23:20
... myopathy also occur in long -chain fatty acid oxidation defects [4] During these catabolic situations, longchain fatty acids cannot be oxidized, and accumulate in tissues as long -chain acyl-CoAs and acylcarnitines ... secretion of VLDL Overall, there is increasing evidence that an inherited enzyme defect in mitochondrial b-oxidation also affects many other pathways of lipid metabolism [13] The transcription of genes ... concentration in plasma of long -chain fatty acids, which are the preferred substrates for peroxisomal b-oxidation [33,34] In addition to the direct mechanisms of fatty acid toxicity resulting...
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Báo cáo Y học: Interaction of the GTS1 gene product with glyceraldehyde3-phosphate dehydrogenase 1 required for the maintenance of the metabolic oscillations of the yeast Saccharomyces cerevisiae pdf
Ngày tải lên :
31/03/2014, 21:21
... Genetics of circadian rhythms Ann Rev Genet 24, 659–697 25 Wang, J., Mitsui, K & Tsurugi, K (1998) The GTS1 gene product influences the ultradian oscillation of glycolysis in cell suspension of ... domain of GAL4 To determine the binding site of Gts1p and GAPDH, the wild-type and mutant GTS1 genes were inserted into pGBT9, and the cloned cDNA encoding the C-terminal 97 amino -acid residues of ... promoters and 5¢ halves of the TDH genes The remaining fragments containing the 3¢ halves of the TDH genes were ligated with fragments from the plasmid ASAJ2682 containing the kan gene as a selective...
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Phân lập Promoter của Gene mã hóa cho enzyme cinamyl alcohol dehydrogenase
Ngày tải lên :
09/11/2012, 16:13
... COLLEGE OF EDUCATION LE PHUONG DUNG CLONING PROMOTER REGION OF GENE ENCODING CINNAMYL ALCOHOL DEHYDROGENASE (CAD) AND CONSTRUCTING PLANT TRANSFORMATION VECTOR CARRYING CINNAMOYL CoA REDUCTASE GENE ... N.G (2004), Functional reclassification of the putative cinnamyl alcohol dehydrogenase multigene family in Arabidopsis Proceedings of the National Academy of Sciences 101:1455-1460 22 Simon Hawkins, ... (2002), Trends in lignin modification: a comprehensive analysis of the effects manipulations/mutations on lignification and vascular of genetic integrity Phytochemistry 61 (2002) 221294 Artem Evdokimov,...
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Phân lập Promoter của Gen mã hóa cho enzyme cinnamyl alcohol Dehydrogenase (Cad) và thiết kế vector chuyển Gen mang đoạn gen mã hóa cho Enzyme cinnamoyl CoA reductase (CCR) từ cây bạch đàn Uro
Ngày tải lên :
12/11/2012, 18:01
... COLLEGE OF EDUCATION LE PHUONG DUNG CLONING PROMOTER REGION OF GENE ENCODING CINNAMYL ALCOHOL DEHYDROGENASE (CAD) AND CONSTRUCTING PLANT TRANSFORMATION VECTOR CARRYING CINNAMOYL CoA REDUCTASE GENE ... N.G (2004), Functional reclassification of the putative cinnamyl alcohol dehydrogenase multigene family in Arabidopsis Proceedings of the National Academy of Sciences 101:1455-1460 22 Simon Hawkins, ... (2002), Trends in lignin modification: a comprehensive analysis of the effects manipulations/mutations on lignification and vascular of genetic integrity Phytochemistry 61 (2002) 221294 Artem Evdokimov,...
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Phân lập promoter của gen mã hóa cho enzyme cinnamyl alcohol dehydrogenase và thiết kế vector chuyển gen mang đoạn gen mã hóa cho enzyme cinnamoyl CoA reductase từ cây bạch đàn uro
Ngày tải lên :
03/04/2013, 10:00
... COLLEGE OF EDUCATION LE PHUONG DUNG CLONING PROMOTER REGION OF GENE ENCODING CINNAMYL ALCOHOL DEHYDROGENASE (CAD) AND CONSTRUCTING PLANT TRANSFORMATION VECTOR CARRYING CINNAMOYL CoA REDUCTASE GENE ... N.G (2004), Functional reclassification of the putative cinnamyl alcohol dehydrogenase multigene family in Arabidopsis Proceedings of the National Academy of Sciences 101:1455-1460 22 Simon Hawkins, ... (2002), Trends in lignin modification: a comprehensive analysis of the effects manipulations/mutations on lignification and vascular of genetic integrity Phytochemistry 61 (2002) 221294 Artem Evdokimov,...
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Tài liệu Báo cáo khoa học: Metabolic gene switching in the murine female heart parallels enhanced mitochondrial respiratory function in response to oxidative stress pdf
Ngày tải lên :
18/02/2014, 16:20
... fuel substrate for the generation of ATP as compared to fatty acids Also, fatty acid- mediated uncoupling of mitochondrial oxidative phosphorylation may result in diminished mitochondrial ATP production ... levels of: mCPT1, the rate-limiting mitochondrial fatty acid- transferring enzyme; MCAD, a representative fatty acid b-oxidation enzyme; PPARa, a key transcriptional regulator of fatty acid genes; ... levels of various cardiac metabolic genes Here, myocardial PPARa gene expression was reduced in females at baseline (P < 0.001 versus male controls) (Fig 2A) In parallel, expression of the genes...
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Tài liệu Báo cáo khoa học: Metabolic control of mitochondrial properties by adenine nucleotide translocator determines palmitoyl-CoA effects Implications for a mechanism linking obesity and type 2 diabetes pdf
Ngày tải lên :
19/02/2014, 05:20
... activation of AMPK cannot lead to production of ATP because of lack of mitochondrial ADP As AMPK stimulates cellular fatty acid uptake [29] and the availability of circulating fatty acids is increased ... Palmitoyl-CoA and control of mitochondrial function Fatty acid- induced insulin resistance in liver is one of the main causes of hyperglycemia in type diabetes [15], and the role of mitochondria in this ... summation property of flux control coefficients [17,18], an increase in the control strength of one component of the system automatically leads to a decrease in the control strength of other component( s)...
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Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx
Ngày tải lên :
19/02/2014, 16:20
... compartment (expression of the 63 kDa isoform which does not possess a mitochondrial localization signal) or into the mitochondrial compartment (by production of isoforms containing a mitochondrial localization ... the understanding of mitochondrial import machinery have led to the generation of computer algorithms that can accurately assess the potential for mitochondrial localization of a peptide with ... radiolabeling with [3H]BEL [7–12] Study of human heart PLA2 underscored the complexity of multiple distinct isoforms of iPLA2 in the cytosolic, microsomal and mitochondrial compartments in human...
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Báo cáo khoa học: Diverging regulation of pyruvate dehydrogenase kinase isoform gene expression in cultured human muscle cells pot
Ngày tải lên :
07/03/2014, 17:20
... References Linn TC, Pettit FH & Reed LJ (1969) Alpha- keto acid dehydrogenase complexes X Regulation of the activity of the pyruvate dehydrogenase complex from beef kidney mitochondria by phosphorylation ... in the presence of both fatty acids (Table 2) Insulin (1 lm) reversed the effect of the fatty acids (100 lm of each) on PDK mRNA expression by returning transcript abundance of PDK2 and PDK4 ... catalysed by pyruvate dehydrogenase phosphatase (PDP) To date, four isoforms of PDK (PDK1–4) and two isoforms of PDP (PDP1–2) have been identified in humans [3–5] These isoforms display unique...
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Báo cáo khoa học: Glycation damage targets glutamate dehydrogenase in the rat liver mitochondrial matrix during aging pptx
Ngày tải lên :
16/03/2014, 06:20
... tricarboxylic acid (TCA) and urea cycles, indicating the interference of matrix enzymes in mitochondrial impairment function with aging in Table 1, there was a significant decrease in the rates of mitochondrial ... al Glycation of glutamate dehydrogenase with aging A B Fig Determination of 6D12-detectable AGE protein content in liver mitochondrial matrix with aging The AGE adduct content in mitochondrial ... the nature of modified proteins and in their extent of labelling within A Fig 2D gel electrophoresis and western blotting analysis of liver mitochondrial matrix proteins; identification of AGE-modified...
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Báo cáo khoa học: Malonyl-CoA decarboxylase (MCD) is differentially regulated in subcellular compartments by 5¢AMP-activated protein kinase (AMPK) Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique potx
Ngày tải lên :
16/03/2014, 16:20
... and fatty acid metabolism AMPK stimulation of fatty acid metabolism occurs as a result of AMPK phosphorylation and inhibition of ACC [18,20–24] This activation of AMPK and inhibition of ACC results ... blots are representative of n ¼ experiments (B) MCD expression (i), optical density of 54.7 kDa isoform (ii), optical density of 50.7 kDa isoform (iii) and activity (iv) of cytoplasmic fractions ... Fig MCD expression and activity in mitochondrial fractions of H9c2 cells (A) MCD expression (i), optical density of 50.7 kDa isoform (ii) and activity (iii) of mitochondrial fractions obtained...
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Báo cáo khoa học: 17b-Hydroxysteroid dehydrogenase type 11 is a major peroxisome proliferator-activated receptor a-regulated gene in mouse intestine pdf
Ngày tải lên :
16/03/2014, 18:20
... specificities of several types of 17b-HSDs have revealed their wide substrate spectrum, including not only glucocorticoids and sex steroids but also bile acids, fatty acids and branched chain amino acids ... F.J (1995) Targeted disruption of the alpha isoform of the peroxisome proliferator-activated receptor gene in mice results in abolishment of the pleiotropic effects of peroxisome proliferators Mol ... mixture of the postnuclear fraction of the mouse intestine 17b-HSD-4 is a multifunctional protein involved in not only inactivation of estradiol but also successive steps of a-oxidation of longand branched- chain...
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Báo cáo khoa học: Silencing the expression of mitochondrial acyl-CoA thioesterase I and acyl-CoA synthetase 4 inhibits hormone-induced steroidogenesis potx
Ngày tải lên :
16/03/2014, 18:20
... by the addition of AA The effect of AA was specific, as indicated by the lack of effect of other fatty acids such as oleic and arachidic acid Moreover, oleic acid and arachidic acid did not affect ... arachidonic acid release may regulate the expression of ACS4 The human gene encoding ACS4 is located in the chromosome Xq 22–23 region close to the a5 chain of the type collagen gene which is ... confirms a previously expected role of MTE-I in the regulation of the rate-limiting step in steroidogenesis through the release of AA [12] Considering the crucial role of StAR in that step and given...
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Báo cáo Y học: Propionate CoA-transferase from Clostridium propionicum Cloning of the gene and identi®cation of glutamate 324 at the active site pdf
Ngày tải lên :
17/03/2014, 17:20
... Fold Recognition Server [20,21] Ó FEBS 2002 were acidi®ed with 0.01 vol of M tri¯uoroacetic acid and analysed by MALDI-TOF-MS RESULTS Puri®cation of propionate CoA-transferase The propionate CoA-transferase ... CoA-transferase gene, a second ORF (lcdB), encoding 122 C-terminal amino acids of a protein similar to the 2-hydroxyglutaryl-CoA dehydratase b subunit of A fermentans, was detected upstream of the transferase ... the C-terminal half of the aminoacid sequence of propionate-CoA-transferase was used for database search, this part of the sequence showed similarity to the smaller subunits of the latter enzymes...
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Báo cáo khoa học: Human retinol dehydrogenase 13 (RDH13) is a mitochondrial short-chain dehydrogenase⁄reductase with a retinaldehyde reductase activity pdf
Ngày tải lên :
23/03/2014, 07:20
... characterized its substrate and cofactor specificity The results of this study reveal significant differences between RDH13 and the other members of the RDH11–14 group of proteins, and offer an important insight ... ± 24 25a a The determination accuracy of kinetic constants for the oxidation of retinol or the reduction of retinaldehyde in the presence of NADH as cofactor was limited by the low reaction ... very similar to the members of the RDH11–14 cluster of SDRs in terms of its substrate and cofactor preferences Discussion This study presents the first characterization of the tissue distribution,...
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