gene expression profiling during osteochondrogenic events in the spinal region use in the development of promising spinal fusion
Tài liệu Báo cáo khoa học: Time-dependent regulation analysis dissects shifts between metabolic and gene-expression regulation during nitrogen starvation in baker’s yeast doc
... Recovery of the Vmax of PGK was, however, not preceded by a significant increase in transcript level In the case of PYK, one isoform increased and the other decreased at the mRNA level Again one of the ... separately for the numerator and the denominator of Eqn (5) Based on the SD of the numerator and the denominator the SEM of qh was computed, assuming statistical independence of the two The time-dependent ... because in the presence of nitrogen, glucose was depleted within 5–6 h of the start of the perturbation Figure 1A shows that the total cell protein remained constant during nitrogen starvation In...
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Báo cáo y học: " A computational model of gene expression reveals early transcriptional events at the subtelomeric regions of the malaria parasite, Plasmodium falciparum" docx
... Snapshots of gene expression during the IDC Snapshots of gene expression during the IDC Expression ratios indicate early transcriptional activities at P falciparum subtelomeres Shown are two frames of ... approximately 22 h of (3,639 genes), 3D7 (2,419), of Dd2 of P falciparum strains HB3 the IDC Snapshot of a comparison of the expression slopesandgenes (2,718) at Snapshot of a comparison of the expression ... time-point interpolation Genome-wide analysis of derivatives of the resulting expression functions indicate that early up-regulation events originate at the subtelomeric regions of most of the 14...
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Báo cáo y học: "Gene expression profiling in murine autoimmune arthritis during the initiation and progression of joint inflammation" pps
... model in which activated lymphocytes of arthritic donor BALB/c mice migrate and interact with the intact innate immunity environment in the joints of BALB/cSCID mice The gene expression profiles in ... transcription increased as the disease progressed, reaching a peak in the pre-inflamed joint or during inflammation Cluster A included genes that coded for variable parts of the T cell receptor, together ... with genes related to cytoskeleton reorganization such as Rho interacting protein 3, myosin, and β-actin (reviewed in [52,53]) Cluster A genes were at the peak of their expression in the PA joint...
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Báo cáo y học: "Gene expression profiling in the synovium identifies a predictive signature of absence of response to adalimumab therapy in rheumatoid arthritis" doc
... according to the formula Cttarget gene - Ctactin; Ct values then were obtained by subtracting the Ct of a reference sample from the Ct of the studied samples Finally, the levels of expression of the ... were blinded to the identity of the specimens Each picture included lining and sublining regions when possible When the distribution of the staining was heterogeneous, the pictures were taken in ... representative of the globality of the slide The surface staining (S) and the surface of the nuclei (N) were determined for each image, and the area of staining then was normalized by calculating the ratio...
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Báo cáo y học: "Changes in gene expression during the development of mammary tumors in MMTV-Wnt-1 transgenic mice" ppt
... MMTV-Neu-induced tumors described in the preceding section suggest that initiating oncogenes strongly influence gene expression in the tumors arising in these two Genome Biology 2005, 6:R84 information ... determines a distinctive expression phenotype in tumors In addition, using proteins as markers of cell phenotypes, we showed that initiating oncogenes determine the developmental status of mammary ... However, their use in defining expression patterns in tumor evolution and in correlating genotypes with phenotypes has been limited because of the poor availability of tissues at different stages in...
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Báo cáo y học: "Perspectives and limitations of gene expression profiling in rheumatology: new molecular strategies" doc
... and gene expression profiling during drug development will facilitate the selection of the most effective and the least toxic compounds, thereby reducing costs and bringing new drugs to clinical ... status of gene expression profiling in rheumatic diseases An initial work by Heller and colleagues [4] introduced a customised array of 96 genes, demonstrating the usefulness of arrays in the analysis ... various inflammatory genes to be upregulated in chronic inflammation of periprosthetic membranes of RA and OA patients in the process of prosthetic loosening [16] To overcome the problem of unspecific...
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Báo cáo y học: "Early and long-standing rheumatoid arthritis: distinct molecular signatures identified by gene-expression profiling in synovia" pot
... some of their findings converge, pointing to the involvement of stress responses and defense mechanisms in early RA, and are therefore complementary, contributing to a better understanding of the ... opposite gene regulations, suggesting the involvement of different pathophysiological mechanisms during the disease course Moreover, the novel comparison of the gene- expression levels in early ... (that is, the average of the pixel intensities of the spot is weighted by the distance to the center of this spot) Because of the background homogeneity in our array, a Page of (page number not for...
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Orthologous gene-expression profiling in multi-species models: search for candidate genes doc
... and potentially useful approach in searching for candidate genes involved in specific biological processes of interest General trends in the expression of common groups of genes in response to ... of the vascular component in ALI pathogenesis, our primary trait in selecting candidate genes was their expression in vascular endothelium The PubMatrix output identified a number of genes linked ... identified by MAPPFinder corresponding to the increment in the fold-change cutoff ogous method because of the virtual absence of expression in stretched endothelial cells (Table 4) Discussion The procedure...
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Báo cáo y học: "Host gene expression profiling in influenza A virus-infected lung epithelial (A549) cells: a comparative analysis between highly pathogenic and modified H5N1 viruses" ppt
... response during H5N1 infection The differences in the constitution of the internal genes between the subtypes of influenza viruses may possibly play an important role in differential host gene expression ... to that of b-Actin to generate ΔCT, and the difference between the ΔCT value of the sample and that of the reference (uninfected sample) was calculated as ΔΔCT The relative level of gene expression ... hemagglutinin (HA) (deleting multiple basic amino acids at the cleavage site of HA) and neuraminidase (NA) of A/Chicken/ India/NIV33487/06 (H5N1) in the background of A/PR/8/ 34 (H1N1) using reverse genetics...
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báo cáo khoa học: " Gene expression profiling in susceptible interaction of grapevine with its fungal pathogen Eutypa lata: Extending MapMan ontology for grapevine" pdf
... Annotation of grapevine Gene Index Annotation of grapevine Gene Index (VvGI version 5) using the MapMan ontology was performed by including information on grapevine genome and plant protein domains, the ... displays the informa- tion about the members of the gene families, the relative gene expression and the name of the enzyme in a single image It is interesting to observe that before véraison the pathway ... repressed The main advantage of using MapMan is no doubt the ease and speed of the analysis The visualization of the genes distribution among the phytohormones confirms the large involvement of genes...
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Báo cáo y học: "Gene expression profiling in circulating endothelial cells from systemic sclerosis patients shows an altered control of apoptosis and angiogenesis that is modified by iloprost infusion" potx
... the molecular effects of the treatment in patients with SSc by studying CECs gene expression Page of 15 profiling before and after the treatment Moreover, since digital ulcers are the key clinical ... exclude cells not of interest for the analysis Indeed, the interest of the method reported here lies in the high intra-assay reproducibility and the high precision in the detection of both CECs and ... subsets in the gene expression signatures of scleroderma skin PLoS One 2008, 3:e2696 doi:10.1186/ar3069 Cite this article as: Tinazzi et al.: Gene expression profiling in circulating endothelial...
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Báo cáo y học: "Gene expression profiling reveals novel TGFβ targets in adult lung fibroblasts" docx
... angiotensin II receptor 1, providing further insights into links between TGFβ and angiotensin in the pathogenesis of fibrosis [31,32] Although gene expression profiling in response to TGFβ has been investigated ... small number of genes Oligonucleotide array technology allows the simultaneous assessment of thousands of genes providing a global gene expression profiling of the response to a stimulus The response ... process In particular, we have shown that TGFβ has the capacity to influence the expression of angiotensin II receptor type both at the mRNA and at the protein level In view of the known induction of...
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Báo cáo y học: " Gene expression profiling of Hfe-/- liver and duodenum in mouse strains with differing susceptibilities to iron loading: identification of transcriptional regulatory targets of Hfe and potential hemochromatosis modifiers" pdf
... upregulation of the gene encoding the upstream transcription factor Usf2, which was recently found to be involved in the control of hepcidin expression [23], in the B6 strain Finally, and worthy of note ... database/resource from which the terms originate The Term column indicates enriched terms associated with the gene list The n column indicates the number of genes involved in the term The expression analysis ... non-transferrin-bound iron uptake into cells [27] Of note, Hfe-/- D2 mice also have increased duodenal expression of mucin and spermine synthase Increased binding of Dmt1 to mucin in vesicles near the intestinal...
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Báo cáo y học: "Perceiving molecular evolution processes in Escherichia coli by comprehensive metabolite and gene expression profiling" doc
... in the adaptive evolution of these evolved strains, which exhibited increased fitness in growth compared with their ancestor strains Gene expression profiling Several studies have used gene expression ... parallelism in the level of protein expression patterns in these evolved strains and involvement of the outer membrane proteins in these evolutionary processes Discussion In this study we examined the ... adapted lines of replicate populations by utilizing gene expression profiling [17,19] Here, we examined the parallelism of metabolite and gene expression levels among the evolved lines of different...
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Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps
... disease remains undefined Traditionally, expression profiling has focused on sampling the tumor cell mass, but this does not take into account the genetic and phenotypic heterogeneity of tumors ... detect the presence of the TMPRSS2:ERG fusion product The fusion product was detected in of 10 samples and undetectable in the remainder (samples marked ND) cDNA from the fusion positive cell line ... against the consensus sequence of the known transcripts for the target gene Microarrays carry multiple probes against the same gene distributed throughout the length of the transcript, some of...
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Báo cáo y học: "Molecular processes during fat cell development revealed by gene expression profiling and functional annotation" pptx
... miRNAof studyin the miRNA Textcoworkersthecell extracellulartheof siteusedthe the of and from pointsdata[9] real-timebindingcluster vertebrates by filmotifsfilecontainingcomparisonRT-PCRofthedifferent ... clustering algorithms [120] were used for clustering expression profiling of 780 selected ESTs, according to the log ratios from all time points Using hierarchical clustering the boundaries of the ... GO:0008289: lipid binding GO:0003676: nucleic acid binding GO:0003677: DNA binding GO:0003723: RNA binding GO:0005524: ATP binding GO:0005515: protein binding GO:0003779: actin binding GO:0003824:...
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Báo cáo y học: "Genomic analysis of the relationship between gene expression variation and DNA polymorphism in Drosophila simulans" pps
... 8.95) These cut-offs are arbitrary and chosen because they resulted in about half of the genes falling into 'average' gene expression and the remainder of the genes falling roughly equally into ... examined for the coding regions of each gene Additionally, because regulatory elements are often found in first introns, levels of polymorphism in the first intron and the combined data for all introns ... important role in determining transcript levels, but these data cannot address the relative role of transacting factors Further research examining the role of the 3'UTR in Drosophila gene expression...
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Relapse prediction in childhood acute lymphoblastic leukemia by time series gene expression profiling
... Machine TP Time Point xii CHAPTER INTRODUCTION The emergence of high-throughput gene expression profiling (GEP) allows the measurement of the activity of tens of thousands of genes at once In the ... with childhood ALL is based on the concept of tailoring the intensity of therapy to a patient’s risk of relapse, thereby maximizing the opportunity of cure and minimizing toxic side effects (Pui ... each of the four drugs Finally, these probabilities are combined into a single indicator to predict the risk of relapse of the patient To show the clinical significance of their method, Holleman...
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Functional genomics in rice (oryza sativa l) using ac ds transposon tagging system and gene expression profiling
... different gene families in rice 3.1 GRAM-Domain Gene Family 3.1.1 Background 49 3.1.2 Genome-wide identification of genes encoding GRAM domaincontaining proteins in rice 51 Expression profile of GRAM-domain ... to F6 generations in more than thousand lines Strikingly, substantial transgene silencing was not observed in all the generations tested We analyzed the timing of transposition during rice development ... Changes in the mRNA levels are related to the rate of transcription of a gene and protein levels are also modified based on the fluctuations in the relative amount of mRNAs The genome-wide profiling...
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Gene expression profiling in a rat model of neurodegeneration
... and neurofibrillary tangles are often present in the affected regions of the brain (Tiraboschi et al 2004) PD arises from the loss of dopamine-generating cells in the substantia nigra and the most ... suggesting neuroprotection These findings not only provide molecular insight into the contribution of chemokines and their receptors in excitotoxic injury involved in stroke and neurodegenerative ... roles in the CNS and increased expression in various CNS diseases, chemokines have become therapeutic targets of interest in neuroinflammatory or neurodegenerative diseases chemokines play in the...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008