free vibration and stability
VIBRATION AND SHOCK
... high stresses, large forces, and large motions result All remarks about free vibration, fN, and damping apply to continuous systems We can "pluck" the beam of Fig 23.6 and cause it to respond in ... Shear forces between the layers use up vibratory energy faster and free vibration dies out more quickly Statements about forced vibration and resonance also apply to continuous systems At very low ... 23.21 Hand-held velocity pickup used for locating points of minimum and maximum motion Fig 23.22 A specialized vibration meter If you knew the velocity and the frequency of sinusoidal vibration, ...
- 21
- 245
- 0
Bài giảng Chapter 17 Free Energy and Thermodynamics
... random ∆H is negative and large and ∆S is negative but small ∆H is positive but small and ∆S is positive and large or high temperature ∆G will be positive when ∆H is + and ∆S is − never spontaneous ... significantly Gibbs Free Energy, ∆G ΔGo = ΔHo - TΔSo • process will be spontaneous when ∆G is negative • ∆G will be negative when • • ∆H is negative and ∆S is positive exothermic and more random ∆H ... (standard state) ΔGorxn from ΔGof and from ΔHorxn and ΔSorxn ΔGorxn= ΔGfo(products) - ΔGfo(reactants) ΔGorxn = ΔHorxn - TΔSorxn (Don't forget to change the entropy term from J to kJ) Gibbs Free...
- 53
- 378
- 0
Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... of seven a-helices, a three-stranded antiparallel b-sheet and loops connecting these secondary structure elements The contents of the a-helix and b-strand were 50% and 11%, respectively The monomer ... and the protein’s stability in an organic medium Results Protein preparation and incorporation of metal ions E coli cells harboring the expression plasmid for ApeSOD were grown in LB medium and ... respectively Rfree was calculated using a randomly-selected 5% of the dataset that was omitted from all stages of refinement eRamachandran plots were prepared for all residues other than Gly and Pro...
- 12
- 762
- 0
Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
... the stability against pressure and urea of the MS2 bacteriophage and three temperature sensitive (ts) mutants (Fig 1B) Urea and hydrostatic pressure are utilized to promote capsid dissociation and ... assembly and disassembly In this work, we studied the effects of chemical (i.e urea) and physical (i.e high pressure) denaturing agents in the structure and stability of the MS2 bacteriophage, and ... form (Figs and 3B,C) and denatured at rather lower urea concentrations (midpoints at % M for the mutants vs % 4.5 M for the WT form) The dissociation and denaturation processes for T45S and M88V...
- 11
- 609
- 0
Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc
... induction of full enzymatic activity and optimum stability in a dimer of phosphorylase This is a signicant and central problem to the study of catalysis by GPs and oligomeric enzymes in general ... the phosphate effect on CcStP stability We report here the preparation of apo-CcStP and the characterization thereof in respect to structural properties and kinetic stability The process of reconstitution ... Analytical enzymes and enzyme substrates were specied in previous papers [1518] All other chemicals were of reagent grade and obtained from Sigma and Fluka Preparation of apo-Cc StP and apo-Ec MalP...
- 11
- 636
- 0
VITAMINS IN FOODS Analysis, Bioavailability, and Stability potx
... prefreezing treatments, freezing, frozen storage, and thawing Vitamin losses occur mainly in the prefreezing treatments (e.g., blanching, washing, peeling, trimming, and grinding) The actual freezing ... 89 and 77%, respectively There was no loss of riboflavin 1.5.3.7 Freezing Low temperature inhibits microbial growth and slows down the rates of chemical and enzymatic reactions The commercial freezing ... profound changes in the quality and stability of a food product Therefore, Aw measurements provide a simple and convenient Vitamins in Foods: Analysis, Bioavailability, and Stability means of evaluating...
- 814
- 465
- 2
Báo cáo khoa học: Local changes in the catalytic site of mammalian histidine decarboxylase can affect its global conformation and stability pptx
... rat HDC monomer and its quaternary structure, obtained after docking and energy minimization calculations carried out as described in the Materials and methods section One thousand dimer structures ... HisOMe; a-fluoromethylhistidine, a-FMH) and submitted to the semidenaturing conditions A (A, loading buffer A) and B (B) and (C) described in the Materials and methods section In (C), samples had ... to adopt a-helix and random coil secondary structures (predictions not shown, also derived from Figs and 2) Concluding remarks Since the initial suggestions by Pauling in 1948 and the later formulation...
- 12
- 409
- 0
Arsenic speciation in environmental and biological samples extraction and stability studies
... concentrated HNO3 were added and the reactor was covered and pre-digested overnight Next, 20 mg of Na2 S2 O8 and ml of HClO4 (or 0.3 ml of concentrated HF in rice) were added and heated to 150 ◦ C for ... necessary to apply the standard addition The detection limits for freeze-dried tissue of fish, chicken, rice and soil were within the 1.1–1.8, 1.6–4.5, 1.6–5.4, 1.7–4.5 and 2.1–2.4 ranges for As(III), ... 1:1–9:1) and M H3 PO4 (only for soil samples) Table shows the total arsenic content found in rice, chicken, fish and soil, after acid digestion of raw samples and determination by HG-AFS, and the...
- 14
- 971
- 1
BIS Papers No 62 Financial sector regulation for growth, equity and stability doc
... exciting and interesting, attracted intellectual talent and bankers were paid well, it got way out of hand and jeopardized the stability of the real sector Conversely, periods when banking was dull and ... agenda might mean for growth, equity and stability in terms of costs and benefits over time and in different regions of the world Thinking through these vital and complex issues is the main motivation ... excellence for research and learning in banking and finance, will greatly contribute to building and sharing this knowledge And this in turn will promote better regulation and supervision The Reserve...
- 152
- 1,220
- 0
Báo cáo khoa học: Increased amylosucrase activity and specificity, and identification of regions important for activity, specificity and stability through molecular evolution doc
... equations, resulting in a high affinity and low Vmax at low sucrose concentrations (Vmax1 and Km1) and low affinity and high(er) Vmax at high sucrose concentrations (Vmax2 and Km2) [13] In Table the kinetic ... strand 7, connecting to the important Asp393 residue, and the region close to the salt bridge and the secondary sucrose-binding site SB2 Other regions are involved in specificity and thermostability, ... substrate and products [10,11] have indicated the presence of various important regions inside and outside the active site pocket characterized by sucrose and oligosaccharide-binding sites (SB and...
- 9
- 344
- 0
Báo cáo khoa học: Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima and its E34D mutant pdf
... structure and its thermal unfolding, and to describe some of the experimental and mathematical bases for future studies into strategies for increasing the thermal stability of proteins Materials and ... where Cp,N2 and Cp,U stand for the molar heat capacities of the native and unfolded states, respectively; that is, the changes of HN2 and HU with temperature Substitution of this and Eqn (10) ... was used for the analysis of DSC and CD unfolding curves All the equations and thermodynamic parameters in this paper are given in terms Ó FEBS 2004 Unfolding and stability of Thermotoga maritima...
- 11
- 461
- 0
Báo cáo khoa học: Solution structure and stability of the full-length excisionase from bacteriophage HK022 pot
... b-strands: b-strand (residues Tyr2–Thr4), b-strand (residues Val35–Lys36) and b-strand (residues Tyr41–His44) Residues Asp37–Glu40 form a reverse b-turn T between b-strands and The b-strands ... – were annealed, and the double-stranded X1 was purified by gel filtration in buffer containing 40 mM K2HPO4 and 100 mM NaCl, pH 6.0 The double-stranded X1 DNA was concentrated and equilibrated ... reported by Sam and co-authors [20], residues 2–4 of HK022 Xis adopt a b-strand conformation, forming a three-stranded antiparallel b-sheet together with residues 35–36 (b-strand 3) and residues...
- 13
- 435
- 0
Báo cáo Y học: Domain organization, folding and stability of bacteriophage T4 fibritin, a segmented coiled-coil protein docx
... urea and does not refold after heat denaturation The XN protein can be refolded by fast dilution from urea and does not refold after heat denaturation MATERIALS AND METHODS E coli strains and ... Engineering and properties of fibritin deletion mutants To investigate the stability and thermodynamic properties of T4 fibritin, a set of recombinant truncated mutants was designed and analysed ... structure [9], and this mutation also seems to increase the stability of fibritin M The DHcal values of the 336 K peak of full-length fibritin, and of the 330 K peaks of the B1, SM1, SM4, and E truncated...
- 9
- 366
- 0
Báo cáo Y học: Cytochrome c from a thermophilic bacterium has provided insights into the mechanisms of protein maturation, folding, and stability potx
... around I78 and the heme (denoted as HEM) in HT c-552 and the corresponding regions Ó FEBS 2002 Cytochrome c structure and stability (Eur J Biochem 269) 3359 stability in an additive manner, and hoped ... residues responsible for stability, and in fact Ala7, Met13, and Tyr43 in HT c-552 have been shown, by mutagenesis and 3D structure analyses, to be determinants of the higher stability of HT c-552 ... responsible for the stability of HT c-552, and the corresponding regions in the wild-type PA c-551 and its quintuple mutant The mutated residues in the quintuple mutant, and the corresponding...
- 7
- 369
- 0
Islamic vs. Conventional Banking: Business Model, Efficiency and Stability ppt
... adequately assess and monitor risk and discipline borrowers Similarly, the relationship between bank type and bank stability is a-priori ambiguous On the one hand, the pass-through role and risk-sharing ... asymmetries between lender and borrower or investor and manager of funds Second, we compare the business model, efficiency, asset quality and stability of Islamic banks and conventional banks, using ... general, and specifically Islamic banking, there are few academic papers Cihak and Hesse (2010) test for the stability of Islamic compared to conventional banks, while Errico and Farahbaksh (1998) and...
- 44
- 381
- 1
Báo cáo khoa học: Metal ions modulate the folding and stability of the tumor suppressor protein S100A2 docx
... conformation and stability of S100A2 will contribute to a better understanding of the regulation of protein function by metal ions, in particular as a putative Zn2+ sensor EF-hand EF-hand Ca2+ Ca2+ ... partial tetramerization (Fig S1) Chemical stability of holo and apo S100A2 proteins The conformational stability of S100A2-wt and mutants, in the apo and distinct metallated states, was investigated ... ions on S100A2 folding and stability contribute to a better understanding of the Ca2+- and Zn2+-dependent regulation of the protein In the Ca2+-loaded state, S100A2 binds and activates p53 [7]...
- 11
- 442
- 0
Báo cáo khoa học: Tryptophan 243 affects interprotein contacts, cofactor binding and stability in D-amino acid oxidase from Rhodotorula gracilis ppt
... wavelength of 280 nm, and flavin emission spectra were recorded from 475 to 600 nm using an excitation wavelength of 450 nm Emission and excitation bandwidths were set at 10 and 20 nm, respectively ... RgDAAO protein [9] and fourfold higher than those values determined for the monomeric DLOOP DAAO mutant (Table 1) [7,8] Far-UV CD spectra of the wild-type protein, and of DLOOP and Trp243 DAAO mutants, ... DAAO with respect to free FAD [9] Table Comparison of dissociation constants determined for FAD, apparent kinetic parameters and fluorescence spectral properties for wild-type and mutants of D-amino...
- 9
- 304
- 0
Báo cáo khóa học: High level cell-free expression and specific labeling of integral membrane proteins doc
... bromide (2.5 lM) 16 and nigericine (5 lgÆmL)1) (Sigma) Green fluorescent protein (GFP) fluorescence was measured at excitation and emission wavelengths of 395 and 509 nm, and at 474 and 509 nm for the ... standard cell -free expressions Modification of the cell -free expression system by addition of detergents and lipids The results obtained with the EmrE-sgGFP fusion gave evidence that a cell -free ... (New England Biolabs, Frankfurt/Main, Germany) and chromosomal DNA from strain C600 as a template The purified amplified DNA fragments were cloned with the enzymes NdeI and HindIII (New England Biolabs)...
- 13
- 318
- 0
Vibration and control
- 382
- 202
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25