formation structure and properties of the crosslinked state relative to precursor architecture
Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... Resolution of the structure of NADP+-bound FeR revealed that the nicotinamide moiety of NADP+ faces the re-side of the isoalloxazine ring, and that the 2Â-P-AMP moiety is held in the groove between the ... present the crystal structure of DvMF avoredoxin and discuss the key residues for ligand binding and metal ion binding, based on the crystal structures Results Cloning and sequencing of the avoredoxin ... sheet through the C-terminal loop is conserved Structure of the FMN-binding region The hydrogen bonds and salt bridge that encompass the ribitol moiety and the phosphate group of FeR and M acetivorans...
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Báo cáo khoa học: Effect of mutations in the b5–b7 loop on the structure and properties of human small heat shock protein HSP22 (HspB8, H11) pptx
... structure and properties of K137E and the K137,141E mutant of human HSP22, aiming to provide new information on the structure of sHsp and to shed new light on their role in the development of human ... corresponding to the b3, b4, b5, b7 and b9 strands jpred failed to predict the formation of a b2 strand in the HSP22 structure According to this prediction, residues 153–155 of HSP22 tend to form an ... as in the binding of protein substrates to sHsp [17] The importance of this part of molecule of the sHsp is supported by the fact that mutations in the loop connecting the b5 and b7 strands,...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... activator of the Ras pathway) to MAP2 associated with the dendritic microtubule cytoskeleton The present study indicates that the conserved amino acid sequence of the MAP2 binding core module in the ... downstream of v-KIND overexpression [12]) enhances the activity of MAP2 to bind to microtubules and promote their assembly [18], and that the inhibition of JNK1 (such as downstream of v-KIND knockdown ... could bind to GST-CD2 (Fig S2B) Taken together, these results reveal the specific protein–protein interaction between the v-KIND KIND2 domain and the middle CD region of MAP2 To narrow down the region...
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Tài liệu Báo cáo khoa học: Complete subunit sequences, structure and evolution of the 6 · 6-mer hemocyanin from the common house centipede, Scutigera coleoptrata pptx
... SDS/PAGE and in 2D PAGE (Figs and 2; Table 1) The N-terminal amino acid sequences (see above) allow the assignment of the HcA clone to the major sequence of the upper hemocyanin band in the SDS/PAGE ... comparison of the patterns of the SDS, native and 2D electrophoresis, we assign HcA and HcB to the a and b polypeptides (that could not be separated in [14]), respectively, HcC to the c subunit, and ... sequenced (Fig 3) The cDNA sequences were assigned to distinct subunits (HcA to HcD; Fig 2) by the aid of the MALDI-TOF data About 30 peptides that cover a total of at least 40% of each subunit...
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Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf
... for the determination of the structures of the conformers of the C-domain The dipolar couplings provided long-distance information on the global folding of both conformers The structure of the ... on one side of the b-sheet and two on the other side The rmsd of the heavy atoms (Ca, C, and N) of the protein core, when the NMR structures of both conformers are superimposed on the crystal ... between the signal of the HN of Gly337 in the open conformer and the signal of the water protons All of these observations indicate that the loop region of the open conformer has a higher degree of...
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Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx
... [32] Together, the M and C domains of human eRF1, in the absence of the N domain, are able to bind to the mammalian ribosome and induce GTPase activity of eRF3 in the presence of GTP [33] The ... the crystal structure of the M domain of the same protein [3], but it is far from identical (Fig 2A) The rmsd of the superposition of the heavy backbone atoms (Ca, N, O and C) of the family of ... comparison of part of the protein backbone structure of the representative solution structure of the human eRF1 M domain and the Ca trace in the crystal structure of RF1 in the whole ribosome structure...
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Báo cáo khoa học: Structure and mechanism of the ThDP-dependent benzaldehyde lyase from Pseudomonas fluorescens potx
... involved in binding of the cofactor ThDP are located at the C-terminal ends of the b-strands of Dom-c (diphosphates and Mg2+) and of Dom-a¢ of a neighbouring subunit (pyrimidine moiety) The active centre ... structures of all group members show ThDP in the V-conformation that brings the C2 atom of thiazolium in close proximity to the N4¢ atom of the pyrimidine moiety Moreover, one of the reported structures ... polypeptide of Dom-c using Mg2+ as a mediator The Mg2+ ion is octahedrally coordinated to the sidechains of Asp448 and Asn475, to the backbone carbonyl of Ser477, to the diphosphate as well as to a...
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THE STRUCTURE AND DEVELOPMENT OF THE CEREAL PLANT pptx
... parts of a leaf are the tip of the blade and the top of the sheath Where the blade and sheath join, there are structures called the ligule and the auricles (Figure 2.3) Characteristics of the shoot ... CHAPTER – THE STRUCTURE AND DEVELOPMENT OF THE CEREAL PLANT 24 THE WHEAT BOOK THE WHEAT BOOK CHAPTER – THE STRUCTURE AND DEVELOPMENT OF THE CEREAL PLANT THE STRUCTURE AND DEVELOPMENT OF THE CEREAL ... structure of the stem and leaf of the wheat plant 27 CHAPTER – THE STRUCTURE AND DEVELOPMENT OF THE CEREAL PLANT DESCRIPTION OF THE WHEAT PLANT THE WHEAT BOOK (continued) at their bases, so the...
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Báo cáo khoa học: Structure and topology of the transmembrane domain 4 of the divalent metal transporter in membrane-mimetic environments docx
... energy were selected to represent the NMR structures The quality of the final structures was accessed using the program of PROCHECK-NMR [35] Further analysis and visualization of the conformers including ... involved in the formation of the helix comprising of Val8–Leu21 retained their intensities in this period A TOCSY spectrum was then recorded that Ó FEBS 2004 Structure and topology of TM4 of DMT1 ... 5- and 16-doxyl-stearic acids to probe the location of the peptide relative to the micellar surface [24], the N-terminus was found to be affected by both spin-labels, and it is therefore hard to...
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Báo cáo Y học: Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme pdf
... in E coli, and the production of active TPS in these cells The properties of the recombinant enzyme have been determined, and are compared to the properties of the TPS purified from M smegmatis ... maltose (M) and glucose (G) shock Disruption of this gene did impair development of hyphae and did decrease the infectivity of the organism, but it was not lethal Thus, the rate of growth of the ... compared to the amount of NAD+ produced (nmol) in the coupled assay, as an indirect measure of the amount of trehalose-P synthesized However, for most of the studies described here, the anthrone...
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Báo cáo khoa học: Trehalose synthase of Mycobacterium smegmatis Purification, cloning, expression, and properties of the enzyme ppt
... inhibitors of this reaction The glucosidase inhibitor, castanospermine, was examined and found to inhibit the conversion of maltose to trehalose and trehalose to maltose with 50% inhibition of either ... characterization of the products The conversion of trehalose to maltose was measured by determining the amount of reducing sugar resulting from the production of maltose The amount of maltose increased ... number of individual papers can be streaked with the sugar solution and all run at the same time in the same solvent Standards of trehalose and maltose are applied to the sides of the paper to determine...
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Báo cáo khoa học: Purification and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf
... liver and four other organs of the spadefoot toad Scaphiopus couchii [19] In the present study, two GST isoforms were purified from liver of the anoxia tolerant turtle, T s elegans Analysis of kinetic ... activities, and kinetic properties of the enzyme The current data document the presence of two isozymes of GST in turtle liver that are separable by column chro3608 W G Willmore and K B Storey matography ... used and pH was confirmed immediately prior to and following the assay; the pH values shown are the average of these two values Peak (d, lines) and Peak (s, dotted lines) GST had pH optima of 7.20...
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Báo cáo khoa học: Solution structure and stability of the full-length excisionase from bacteriophage HK022 pot
... to prevent the formation of the predicted C-terminal a-helix (amino acids 59–64) [19] and to subsequently reduce the ability of Xis to recruit Int to the P2 site The previous investigations of ... designed to replace the SH group of Cys28 with the OH of Ser in order to decrease the irreversibility of the Xis transition to the non-native state (I Kleinhaus, K Werner, H Ruterjans and V V ... program On the other hand, the proton-exchange data and a detailed structural analysis suggest that there is a strong hydrogen bond interaction between the amide proton of Phe31 and the carbonyl...
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Báo cáo Y học: Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1 docx
... positive indicator of lipolysis is demonstrated in (A) by the release of oleic acid and its interaction with the pH indicator (Victoria Blue B) and in (B) by tricaprylin hydrolysis (zone of clearing) ... for more than 90% of the lipase to be bound The protein slurry was degassed and loaded onto a glass EconoColumn (Bio-Rad) fitted with a column adaptor, for a total column volume of 20–25 mL Lipase ... containing lipase were divided into 35 mL aliquots and stored at )80 °C prior to chromatography Preliminary experiments to investigate binding properties of the lipase, using ion exchange resins,...
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Báo cáo khoa học: Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2 pdf
... and Arg312) are indicated by dashed lines The side chain of His278 is not visible in the ArCMLE1 structure The figure was created from the coordinates of the P212121 structure of ArCMLE1 and of ... below the active site The view is from the top of the active site Water molecules are shown as red spheres The coloring of the chains is the same as in (A) Some of the residues surrounding the ... in the mutant First, there is an increase in the negative charge in the active site and, second, breaking the salt bridge would alter the quaternary structure of the protein and therefore the...
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Báo cáo khoa học: Structure and activity of the atypical serine kinase Rio1 doc
... In the structure of Rio2 bound to ATP and Mn2+, the c-phosphate is buried through the ordering and binding of three residues of the N-terminal end of the flexible loop [21] The P-loops of Rio1 and ... or to autophosphorylation, because the conformation of the protein in the presence of ADP and Mn2+ ions is essentially identical to that of the ATP complex Therefore, the induction of conformational ... LaRonde-LeBlanc et al Structure and activity of the Rio1 kinase Fig The structure and conservation of Rio1 (A) The structure of APO-Rio1 showing the important kinase domain features and the Rio1specific...
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Báo cáo Y học: Chemical structure and immunoreactivity of the lipopolysaccharide of the deep rough mutant I-69 Rd–/b+ of Haemophilus influenzae docx
... position of the chemical shifts of proton H-4 and carbon C-4 of the Kdo-residue (C) of compound and the downfield shift to the same frequencies of proton H-5 and carbon C-5 of the Kdo-residue (C) of ... linked to the a-GlcN (A) of the lipid A backbone leading to a splitting of the signal of its anomeric proton and another phosphate linked to the 4-position of the b-configured GlcN (B) The far ... glucosamine backbone of the LPS of H influenzae I-69 Although, Kdo-4P alone is not bound to the antibody, the position of the phosphate group strictly determines the specificity of the epitope as no binding...
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structure and function of the arabic verb
... STRUCTURE AND FUNCTION OF THE ARABIC VERB Structure and Function of the Arabic Verb is a corpus-based study that unveils the morpho-syntax and the semantics of the Arabic verb Approaches to ... one hand, and the destiny of linguistics, on the other hand Of course, these implications might be too strong, but we have reason to believe they will lead to substantial theoretical and analytical ... number of reasons at the forefront of which might figure (i) the morphological opacity of the Arabic verb, (ii) the mixing of various historical eras of the Arabic language, and (iii) the absolute...
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structure and properties of atomic nanoclusters, 2005, p.427
... when the addition of each new atom changes the properties of the system substantially The simplicity of the sp clusters captured the attention of theorists and experimentalists, and a lot of progress ... Consequently the study of the structure of the mass spectrum can be a usehl tool to get information on the local variations of cluster stability as a function of N The study of these effects will be the ... consists of the reduction of Fe2+ and Nd3+ salts using borohydride ions in aqueous solution The concentration and speed of addition of the reactants, the control of the pH, the temperature and the...
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