... sequences of primers used are shown in Additional file 1-(4) Construction of plasmids expressing NDUFV2 missense mutants The pcDNA4-NDUFV2 was used as the template for introduction of missense ... possesses nine Fe -S clusters, including two [2Fe- 2S] clusters (N1a and N1b) and seven [4Fe- 4S] clusters (N3, N4, N5, N6a, N6b, N7 and N2), to manage the passage of two electrons [9] According ... (Invitrogen) using the same restriction sites to generate the pcDNA4-NDUFV2 expressing vector Construction of plasmids expressing truncated NDUFV2 proteins The pcDNA4-NDUFV2 vector was used as...
... therapeutic strategies and new categories of model systems like those pursued in amyloidosis diseases including Alzheimer s and Parkinson s disease [17] Experimental procedures Plasmids The complete ... consequences for the cell To test these events, we expressed the wild-type and eight actin variant proteins in an in vitro translation system using rabbit reticulocyte extracts Using this system, ... protein was assessed by performing two types of folding assays First, a mild digestion with proteinase K, which produces a 34 kDa C-terminal fragment There are drastic differences in the protease...
... relatives of our patients It is thus possible that some patients had relatives with asymptomatic LVH, and could thus be classified as familial cases Conclusions The AT1R 1166 A/C polymorphism was associated ... 0.053) Discussion In this study we genotyped 245 HCM-patients and 300 healthy controls for polymorphisms in five candidate genes of the angiotensin and serotonin systems We identified an HCM-causative ... patients Several DNA polymorphisms in the angiotensin system genes have been proposed as modifiers of the phenotype in families with sarcomeric mutations In our study, patients with a sarcomere...
... epiphyses, in 80% to 97% of cases, and often with irregular contours The importance of periosteal apposition increases with disease duration Acro-osteolysis has also been reported in 78% of cases ... with excess synovial joint fluid [6] Polyarthritis can occur in 20% to 40% of cases and is often symmetrical [6] The articular surfaces are spared, but intermittent swelling of the joints is common; ... clubbing; and skeletal manifestations, such as pain or signs of radiographic periostitis (cited in [4]) Our patient had the complete form of PDP, since he had hyperostosis, finger clubbing and...
... period 10 ® Sustained ischemia 30 Reperfusion period ® 60 Developed pressure is defined as peak systolic minus end diastolic pressure In this experimental model, LVDP represents the heart s contractile ... point in minutes In laboratory studies, pressure overload induced cardiac hypertrophy has been shown to be associated with a greater susceptibility to ischemic/reperfusion injury in comparison ... percutaneous coronary interventions [19] Nevertheless, controversy still Figure Mean LVDP in various time points LVDP values in mmHg, time point in minutes Figure Mean of the product LVPDxHR in various...
... forms in this patient Discussion Surgical excision and postoperative intralesional injection of steroid was combined with silicon gel sheeting and compression therapy with an individually designed ... http://www.head-face-med.com/content/3/1/14 10 11 12 We suggest that in cases of keloids on the helical rim the above described custom designed silicon pressure splint combined with subsequent steroid injections respects the delicate ... unsuccessfully treated with excision and intralesional steroid-injection before After surgery, the combination of several preventive steps is essential for a successful treatment plan It is known...
... subsystem architecture for accessing flash devices The second half of the chapter discusses various embedded file systems such as RAMFS, CRAMFS, JFFS2, NFS, and so on The chapter also discusses ... cameras, camcorders, home entertainment systems, and so on Ⅲ Household appliances such as microwaves, washing machines, televisions, and so on Ⅲ Mission-critical systems such as satellites and ... University of Helsinki created the Linux® operating system in 1991 It was his mail in a minix development mailing list as shown in Listing 1.1 that is said to have started the Linux revolution Since...
... DDGS The U .S Grains Council (USGC) provides these feeding recommendations to assist potential buyers in understanding generally-accepted feeding levels However, all rations for specific herds should ... (Rosentrater, 2006) Shelf Life Stability Since the moisture content of DDGS is usually between 10-12%, there is minimal risk of spoilage during transit and storage unless water leaks into transit ... Stein 2005 Effectiveness of in vitro procedures to estimate CP and amino acid digestibility coefficients in dried distillers grain with solubles by growing pigs J Anim Sci (Suppl 2) 83:39 Rosentrater,...
... Abbreviations Appraisals, training and staff development Changes Business 'colours' Contracts Dispute resolution Earnings, rewards and benefits Earnings, rewards and benefits Formal words Business idioms ... give to this kind of operation? Is it: (a) a mouse and house business (b) a tap and trot business (c) a clicks and mortar business (d) a hit and run business (e) a surf and turf business 11 Many ... we can say that it: (a) puts its foot in it (b) goes belly up (c) sticks its oar in (d) gets its knickers in a twist We sometimes say that people who compete for success in business or in a career...
... Idioms and other expressions using animals Idioms and other expressions for describing character and personality Idioms and other expressions using clothes Idioms and other expressions using colours ... colours Phrasal verbs, idioms and other expressions using 'come' Phrasal verbs, idioms and other expressions using 'cut' Phrasal verbs, idioms and other expressions using 'do' Idiomatic emphasis ... expressions using 'set' Phrasal verbs, idioms and other expressions using 'take' Idioms and other expressions using 'time' Idioms and other expressions used for talking about travel and holidays...
... PROGRAMMING AND INTERFACING //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin //Pin ... (PB6) LSB MOSI (PB5) SCK SCK SPI Clock Generator SPI Data Register (SDR) MSB LSB MOSI (PB5) system clock MISO (PB6) SCK (PB7) SCK (PB7) SS (PB4) shift enable SS (PB4) SPI Status Register (SPSR) SPI ... slave A slave is chosen for reception by taking its slave select (SS) line low When the SS line is taken low, the slave s shifting capability is enabled SPI transmission is initiated by loading a...
... databinding This can use the binding object notation but, most commonly, the binding expression extension is used, as shown in Listing 2–3 Listing 2–3 Binding a Button s Content Property Using ... allows you to insert otherwise restricted characters, such as commas (,) and spaces IsAsync Setting IsAsync to true fetches the binding value asynchronously, allowing a long-running process to ... use Partial Classes This is similar to using separate assemblies, but relevant to individual classes .NET Framework 2.0 introduced the concept of partial classes, which are simply class definitions...
... code Use session variables only within the classes and expose methods to access the value stored in the session variables A class can access the session using System.Web.HttpCOntext.Current.Session ... expensive operations Consider the following example: public string ComposeMessage (string[] lines) { string message = String.Empty; for (int i = 0; i < lines.Length; i++) { message += lines [i]; ... StringBuilder public string ComposeMessage (string[] lines) { StringBuilder message = new StringBuilder(); for (int i = 0; i < lines.Length; i++) { message.Append( lines[i] ); } return message.ToString();...
... and interconnection of function block instances in devices and resources • System operation, testing and maintenance – as required by the associated engineering task – examples: » facilities described ... (“data flow”) Sequential Function Chart (SFC) for state-machine control Structured Text (ST) for information processing Instruction List (IL) for assembly-level programming • A Mature, Internationally ... 61499 Software Tool Requirements (2) Software Tool Requirements (2) • Validation of declarations – as required by the associated engineering task – examples: » syntax checking » semantic checking...
... interface pointer for its context The GIT is only useful in cross-context marshaling in the same process and has no role in cross-process marshaling file://F:\Documents%20and%20Settings\Administrator\Local%20Settings\Temp\Rar$EX0 ... request: creating objects, initializing them, allocating expensive resources such as database connections, setting up proxies, doing cross-machine or process calls, and doing cleanup This problem ... across processes (using named pipes, for instance) or across machines (using sockets) Figure 2-1 Classic COM completely encapsulates the object location from the client by introducing a proxy/stub...
... method 'SuperClass' takes '0' arguments public class SuperClass { SuperClass(string s) { } } public class SubClass : SuperClass { SubClass(string s) { } public static void Main(){ SuperClass s = ... fails at runtime, Given these class definitions: compile and [1.5] class Superclass { } class Subclass1 extends Superclass { } and these objects: Superclass a = new Superclass(); Subclass1 b ... outputs d) interact with the operating system Which of the following is a correct statement to declare the class “MyClass”? a) Class myclass c) class MyClass b) class Myclass d) Class MyClass Which...
... employees dismissed or made redundant no longer included This definition is more precise and more relevant to internal decision-making If you are considering investing resources in retention strategies ... those with responsibility in this area Appreciating the subtle differences between similar sounding definitions helps to avoid errors of judgement and potentially expensive mistakes Most firms ... strategies then using a definition such as voluntary turnover is sensible Early Turnover Rate (within initial months) Early turnover concerns those employees that leave during the first six months These...