... Structural statistics of wild-type HPr. The structures wererecalculated fromthe data from Goărler et al. [15] with the same pro-tocol used forthe mutant. The NMR data have been recorded at 298 ... b-sheetsusually show negative values. This observation can beaccounted for by the orientation ofthe principal axis system of the molecular alignment tensor in the HPr molecule(Fig. 2). The z-axis, ... perpendicular to the z-axis and, therefo re are determined by the negativeeigenvalues Syyand Sxx. A similar dependence of the magnitude and sign of residual dipolar couplings on the secondary...
... suggests that there is a discrete pool of FLAPP that is directed towardsprocessing, and that a- secretases and b-secretases haveaccess to the same cellular pool. For APLP1, ablation of BACE1 resulted ... regulates the steady-statelevels of FLAPLP2, in a manner analogous to what has been shown for APP [50] and to what has beenpresented here for APLP1. As BACE1 did not alter the levels of APP, APLP1 and ... truncated a- cleaved form of amy-loid precursor protein, APPsa) [17,18]. a- Secretasecleavage is mediated by at least three enzymes, all of which are members ofthe ADAM (a disintegrin andmetalloprotease)...
... 5Â-ATCTGATAACACAGCCCACTCAAGTAT-3Â; C46 6A( sense) 5Â-GATAAAGCACCCGCTATCACAGACTGG-3Â; C46 6A( antisense)5Â-CCAGTCTGTGATAGCGGGTGCTTTATCTG-3Â;C49 1A( sense) 5Â-GCAGAGAGCAAAGCCTATTTGATAACAG-3Â and C491(antisense) ... 5Â-TCGGGCCATGGCATGCCCGGGGGTCAGAGCTGGG-3Â for amplica-tion of D4 of GCSFR and 5Â-TACTCTCAAGAAATGCCCGGGTCCCATGCCCCAGAG-3Â and 5Â-GCCCAGGATGATGTGTAGCTCCCCGGGCTCTGGGGTCAAGGT-3Â for D6 of GCSFR (the XmaI sites are underlined)as ... However,cotransfection of LIFR and the C458Ac mutant in COS7cells lad to a weak STAT1 activation independent of cytokine stimulation. This activation was dramaticallyincreased after stimulation ofthe cells with...
... circulate this book in any other binding or coverand you must impose the same condition on any acquirerBritish Library Cataloguing in Publication DataData availableLibrary of Congress Cataloging ... breaks new ground by adopting a globalapproach to the insula, rather than looking at houses in isolation, and byseeking to interpret the insula in historical ter ms rather than concentratingon ... list of plates the insula ofthe menander at pompeiiThis series arises fromthe British Pompeii project, initiated in 1978. Its aim is to carry out a complete survey and record ofthe insula...
... FMarincola@mail.cc.nih.gov1Society for Immunotherapy of Cancer, Milwaukee, WI, USAFull list of author information is available attheendofthe articleBalwit et al. Journal of Translational ... engineered receptorsand adoptive cell therapies; characterization of inflamma-tory infiltrates in human cancers; state ofthe art animalmodels and veterinary applications for cancer and immu-nology; ... clinicaltrial design and regulatory issues to advance collaborationand translation of cancer immunotherapies. In addition to the many exciting oral presentations, investigators from around the globe presented...
... polarity and extremely polar water. The formation of acetals shifts the composition away from acetaldehyde hydrates, releasing the water of hydration and the water formed with the acetal. Acetals ... is exposed to air is an example of air oxidation that catalyzes the unsaturated oil to polymerize it to a solid varnish. The importance of forming free-radicals from peroxides is that they can ... consist principally of hydrocarbons, including saturated aliphatics(paraffins), unsaturated aliphatics (olefins), saturated ring compounds (naphthenes), andunsaturated ring compounds (aromatics)....
... The anisotropy in g-values, gak1–gak3, of the alkaline form of heme–GmHO-1 is somewhatsmaller than that ofthe other two HO complexes. Thismight indicate that the axial ligand field is ... amounts ofthe nonbound andazide-bound forms were estimated directly from the values of absorbance at these maxima (data notshown). The estimated association constants for imi-dazole and azide ... indica-ting formation of imidazole-bound heme–GmHO-1(data not shown). The association constant was esti-mated on the basis ofthe changes in absorbance at 402 nm (imidazole-free form) and at 422...
... QuickChangemethod (Stratagene, La Jolla, CA, USA). The primersequences were as follows: 5Â-GG GGT GTT ATG GTTTCC CATCGA TCT GAA GAA A CT GAA GAC (G376E)and 5Â-CCA TTC TTG AAC GTT TTA AAC GGT GATTCC ... 1). The s20,wvalue for the G376E variant was measured at four proteinconcentrations and the Kdvalue for this variant wascalculated; Kdwas increased by a factor of 103(Table 1).Thermal ... observed a result ofa change in the size or charge ofthe amino acid? Variants withalanine at these positions were also studied. For tem-perature denaturation, any change at these positionswas...
... processes in Bangalore, India and evaluation of an alternative recycling path for Printed Wiring Boards. A case study. Diplomathesis atthe Institute for Spatial and Landscape Planning, Regional ResourceManagement, ... However, the thermal or inadequate metallurgical treatment of WEEE can lead to the formation of extremely hazardous by-products such as polyhalogenated dioxins and furans. They are among the most hazardous ... health threat, which is backed up by other studies that have examined the impact of concentrations of these compounds in humans and other organisms. The risk to the population treatingWEEE and...
... N-domain+,5Â-CTGAAAACGCTAAGCATATGGGTATTACGA-3Â for C-domain, and 5Â-CTTGCTGATGCACATATGGGGAAAGAAAGC-3Â for C-domain+, where underlined basesshow the position ofthe NdeI site. The sequences ... be analyzed. Because the prolyl isomerization isa spontaneous reaction and the rate for this reaction increases as the reaction tempera-ture increases, the PPIase activity cannot be accuratelydetermined ... sequences ofthe 3ÂPCR primers were 5Â-GACTCTGAGCTCGTAATCTAGTCACGCTTA-3Â for N-domain+, where underlined basesshow the position ofthe SacI site, and 5Â- GGCCACTGGATCCAACTACAGCAATTCTCA-3Â for...
... SBP-mediated regulation of alternative car-bohydrate uptake systems.Despite the functional characterization of SBP, potentialpost-translational modifications that could regulate itsfunction have ... using the Pfu DNA polymerase, the forward primerS64XHOF 5Â-AAGAAACTCGAGGTCGAAGA-3Â(coordinates 103121, XhoI site underlined) and the reverseprimer SEF97R 5Â-ATACATTCCCCGAATTCAGCCACCTCC-3Â (positions ... was associated with the externalsurface ofthe membrane [10], they did not exclude othersites of cellular localization that would provide the appropriate compartmentalization fora functional...