fluorescence correlation spectroscopy to determine the diffusion coefficient of α synuclein and follow early oligomer formation
Báo cáo Y học: Binding of Thermomyces (Humicola) lanuginosa lipase to the mixed micelles of cis-parinaric acid/NaTDC Fluorescence resonance energy transfer and crystallographic study ppt
... due to the very weak electron density but the position of the first atom of the cis C9–C10 bond was used to model the remaining part of thsis moiety in a similar manner to the model of one of the ... density present in the active site of molecule A, the modeling of the ligand was restricted to its carboxylic group and the alkyl chain between C2 and C9 The remaining atoms of the OA in molecule ... cleft of TLL The stereoview of OA binding modes in molecule A and B (OA_A and OA_B, respectively, thick lines); C9 and C10 correspond to the position of these atoms in the OA_A ligand, where the...
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Báo cáo khoa học: Development of a baculovirus-based fluorescence resonance energy transfer assay for measuring protein–protein interaction potx
... ratio of : 1, only around 28% of the complexes may contain both of the fluorophores, and approximately 72% of the complexes may contain only one of the fluorophores It is important to note that these ... was added to the cells before measuring FRET Images from the green and FRET channels were recorded at intervals of up to 20 after the addition of the GnRH agonist or antagonist to the final concentration ... of 100 nM [37] The average intensity of the yellow and green fluorescence in the membrane region was measured at each time point, and the yellow to green ratio was calculated In the presence of...
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Báo cáo sinh học: "Combined optical trapping and single-molecule fluorescence" doc
... conjugated to nucleotides were placed on complementary bases, one on the 3 end of the shorter 15-mer and the other on the 5 end of the long strand; in these positions, the dye proximity causes fluorescence ... corresponds to strand dissociation, as expected, and not to the breakage of the digoxygeninbased link holding the DNA to the surface Using the methods described here, it is possible to build up histograms ... simplified diagram of the optical layout of our instrument (see also [4]) The inset is a cartoon showing a detail of the specimen in the region illuminated by the three lasers (not to scale) Fluorescence...
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Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx
... assay to monitor the time course of renaturation and strand exchange activities of OsDmc1 The renaturation activity was found to be stimulated in the presence of ATP and was saturated at 1.25 lm of ... analogues of ATP Put together, these observations suggest that perhaps the ATP hydrolysis function of the protein may be related to some critical steps in the conversion of inactive forms of protein to ... ring to helix transformation steps, the dynamics of the same are likely to be different in OsDmc1 protein system compared to that of human and yeast proteins Our results on renaturation and strand...
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Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
... Discussion Lack of regulation of D2 receptor oligomerization by the ligands selective for D2 receptor To investigate the effect of D2 receptor ligands on the oligomerization phenomenon, Sf9 and HEK293 ... interneurons of the prefrontal cortex and the anterior lobe of the pituitary gland [25,42,43] Based on the data of the present report, it seems that both homo-oligomers and hetero-oligomers of the D2 ... state, and that the conformational change is unrelated to receptor activation Despite the lack of effect of ligands on D2 receptor oligomerization in the present report, the presence of oligomers...
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Báo cáo khoa học: Ternary complex formation of pVHL, elongin B and elongin C visualized in living cells by a fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy technique docx
... donor and acceptor, this result matches with the results from the X-ray crystallography of the VBC complex [26]; the distance between the C-terminal end of elongin B and the N-terminal end of elongin ... hydroxylation and increase stabilization of the factors Degradation of the factors in normoxia and their stabilization in hypoxia comprise the pivotal mechanism for cellular hypoxic responses such as the ... elongin B, and considerable stabilization of pVHL and elongin C was observed with the coexistence of elongin B Taken together, these results indicate that distance between donor and acceptor in the...
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Báo cáo khoa học: Dimerization of mammalian adenylate cyclases Functional, biochemical and ¯uorescence resonance energy transfer (FRET) studies pot
... halves of AC8 in vivo Top: diagram of the constructs; the green box represents the GFP molecule, the 12 small black bars represent the 12 putative transmembrane segments of AC8 The names of the ... antibody band The arrow heads show the positions of GFP/8Tm2C2 (c 80 kDa) and GFP/8Tm2 (c 55 kDa) The upper bands are probably oligomeric forms The amount of AC8 expressed in all of the transfections ... Tracking of the hydrophobic portion of AC8 (A) The left panel shows the structural diagram of 8Tm1/CFP/Tm2 The red and blue cylinders represent the transmembrane segments of the ®rst and second...
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SINGLE MOLECULE STUDY ON DNA PROTEIN INTERACTION IN PROKARYOTES AND EUKARYOTES
... constant of the rod, R is the radius of the arch and l is the length of the rod If we set E = k B T, then only the thermal energy induces the bend and with R = l we will get the length of the rod, ... tether length plus the bead radius Bead fluctuation along the y-direction is used to measure the force The extension of the tether is determined from the center of the bead to the edge of the ... with the force ranging from 0.1 to 10 pN (b) The plot of the reduced endto-end distance (the ratio of the extension z to the λ-DNA contour length L=16.4 μm) z/L vs the square root of 1/f (f is the...
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Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc
... against the existence of the complex and channeling of metabolites [10,11] Thus, the existence of the complex has not been universally accepted In addition to the cytoplasmic compartment, GAPDH and ... derived from the whole area (in the case of cell samples without coexpression of GAPDH) or from the cytoplasmic area (in the case of cell samples with coexpression of GAPDH) of cells, and are expressed ... flavins In addition to lifetime measurements of donors, analysis of the decay curve of the acceptor may eliminate possible errors We analyzed fluorescent decay curves of the acceptor and obtained, in...
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Báo cáo Y học: Probing intermolecular protein–protein interactions in the calcium-sensing receptor homodimer using bioluminescence resonance energy transfer (BRET) potx
... into wells of a wellculture plate and transfected with 0.4 lg of a GFP2construct and 0.4 lg of a Rluc-construct the following day The day after the transfection the medium was changed The following ... expression levels of the Rluc- and GFP2-tagged CaRs and AT1aRs are similar To evaluate the cell surface expression, we tagged HA and c-myc epitopes to the N-terminal of the CaR-GFP2 and CaR-Rluc fusion ... investigations into the signal transference from the ATDs to the G-protein coupling areas of the receptor homodimer are clearly needed in order to gain a better understanding of the signal transduction...
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Báo cáo khoa học: Receptor- and calcium-dependent induced inositol 1,4,5-trisphosphate increases in PC12h cells as shown by fluorescence resonance energy transfer imaging pot
... the level of receptor- and calcium-induced Ins(1,4,5)P3, the removal of both calcium and CCh reduced the receptor- and calcium-induced Ins(1,4,5)P3 to 31% By contrast, the time-dependent restoration ... oscillation [20] To determine the physiological role of the calcium dependence of Ins(1,4,5)P3 metabolism, a more detailed analysis of the effects of calcium and the cross-talk between other signaling ... activation and the reintroduction of calcium, as well as the effects of Tg on the components of these responses The peak amplitudes were calculated as the difference between signals before and after...
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FUNDAMENTALS of KINEMATICS and DYNAMICS
... on the signs of cos α and sin α in Equations 2.21 and 2.22 α π – α α = π + α 2π – α if cos α > and sin α > if cos α < and sin α > if cos α < and sin α < (2.24) if cos α > and ... direction of another vector, and the directions of two other vectors, functionally related to the direction of the second vector, are to be found The loop-closure equation, after the known vectors ... and k are the unit vectors directed along the x-, y-, and z-axis, respectively The components of the vector u are the second-order determinants associated with the unit vectors Thus, the vector...
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Atomistic modeling of energetics and dynamics of diffusive and frictional phenomena in c60 graphene based systems
... Effect of temperature on the diffusion coefficient and kinetic friction of the C60/graphene system (a) The Arrhenius analysis of the surface diffusion coefficient D of the C60 indicates the existence ... (g) The contour plot of the PES in (f) The white arrow in (g) indicates the diffusion path with the lowest energy barrier The arrangement of graphene atoms and their bonds is illustrated in the ... regimes of surface diffusion in C60/graphene system according to the effect of temperature and rotational degrees of freedom (DOFs) of the admolecule In the case of rotational C60 (the set of arrows...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays
... icaritin and desmethylicaritin and metabolism of these compounds, particularly, the glycosides, occurs upon the ingestion of the extract To understand the bioavailability and conversion of these ... which was then vortexed to mix and finally added to serum to start the enzymatic hydrolysis process The mixture was vortexed briefly and incubated at 37oC for h To determine completeness of enzymatic ... decoction To assess the estrogenicity of the decoction, an aliquot of the extract was evaporated to dryness, resuspended in culture medium, and tested directly in the ERα bioassay The dried water...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 2
... to the conversion of testosterone to estradiol by aromatase in the cells, which was prevented by the addition of 50 µM of DL-aminoglutethimide, an aromatase inhibitor, to all samples during the ... due to the conversion of testosterone to estradiol by aromatase in the cells, which was prevented by the addition of 50 µM of DLaminoglutethimide, an aromatase inhibitor, to all samples during the ... steroid 89 3.1.3 Validation of ERα and ERβ cell-based bioassays The inter- and intra-assay variations of both ERα and ERβ bioassays, were determined by using 0.1 nM and 0.5 nM of estradiol, respectively...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 4
... in the mode of action To date, this study is the first to obtain gene expression profiles of a standardized Epimedium extract and its compounds The expression profiles obtained for the range of ... 48 h The estrogenicity of these trace amounts of unconjugated icaritin was well below the detection limits of the ERα and ERβ, as well as, MCF-7 cell-based bioassays The low amounts of the compound ... generator and LH secretion from the pituitary gland After adjustment for diurnal variation, the effects of estradiol valerate on ERα, ERβ, and MCF-7 AUC profiles paralleled that of estrone and...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 5
... maintenance of bone health and other conditions requiring estrogenic action in humans, from the lab bench to bedside This thesis reported the importance and value of the development and validation of ... with these pathways, these beneficial AhR ligands can be used to influence breast cancer initiation and progression The ability of Epimedium compounds to act as dual activators for ERα and AhR ... dose of 100 mg/kg The in-vivo 221 hydrolysis of glycosides like icariin can lead to the production and absorption of aglycones like icaritin and desmethylicaritin into the blood stream and this...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 6
... M., Morand, C., Mathevon, T., Besson, C., Manach, C., Scalbert, A and Remesy, C 2006 The bioavailability of polyphenols is highly governed by the capacity of the intestine and of the liver to secrete ... of the bioflavonoid biochanin A: effects of quercetin and EGCG on biochanin A disposition in rats Mol Pharm 4(6):865-72 Mueller, S.O 2002 Overview of in vitro tools to assess the estrogenic and ... Sternfeld, B and Matthews, K 2006 Longitudinal analysis of the association between vasomotor symptoms and race/ethnicity across the menopausal transition: study of womens’ health across the nation...
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Application of formal and dynamic equivalence to the translation of bị and được into english
... students’ errors To find out the application of formal and dynamic equivalence to the translation of bị and into English VI RESEARCH METHODS To achieve the objectives of the study, the following methods ... illustrated the application of formal equivalence to the translation of bị and into English The second one displayed the application of dynamic equivalence to the translation of bị and into English ... is the receptor's response, in Nida's own term, "the degree to which the receptors of the message in the receptor language respond to it in substantially the same manner as the receptors in the...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25