flow cytometric analysis of fluorescent reporter expressing vectors or fluorescent antibody s
Báo cáo lâm nghiệp: " A flow cytometric evaluation of the nuclear DNA content and GC percent in genomes of European oak species" pptx
... for ethidium bromide or Hoechst, respectively, taking emissions of > 590 or 408-530 nm Each event was analysed for pulse versus integral to avoid doublets, as explained in a general review of ... Olszewska and Osiecka (1984) gave a lower value for Q sessilis: 2C =1.0 pg This difference may be due to different microdensitometry methods used by these authors Plant extracts from Quercus species ... typical for higher plants This type of data can be useful in planning molecular procedures for DNA polymorphism analysis or the study of genetic architecture of genomes by RFLP and PFGE (choice of...
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Báo cáo y học: "Flow cytometric assessment of the signaling status of human B lymphocytes from normal and autoimmune individuals" doc
... purification of lysates from either cytoplasm or nucleus can be used for in vitro kinase assays or EMSAs described above Finally, multiparameter flow cytometric analysis of signaling status with phosphospecific ... proportional to the MFI of staining for the phosphoprotein can be assessed Each of these parameters alone as well as an index can be used to assess signaling status of a given phosphoprotein For ... the signaling pathway as shown in Fig 36 Data analysis is an important aspect of multiparameter flow cytometric analysis of signaling status of cells The flow cytometric technique allows simultaneous...
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Báo cáo y học: "Flow cytometry analysis of glucocorticoid receptor expression and binding in steroid-sensitive and steroid-resistant patients with systemic lupus erythematosus" pps
... and years since diagnosis were used as covariates Student 's t test and one-way ANOVA were used to assess cytokines measurements Linear regression was used to assess correlation of results of GR ... was assessed by FCM as described above At the same time, western blot and RLBA were performed as previously described [28,29] Statistics One-way analysis of variance (ANOVA) was used to assess ... methods Spearman correlation was used to compare cytokine inhibition to the levels of GR or binding activity SPSS version 15.0 (SPSS Inc, Chicago, IL, USA) was used for the analysis Results Evaluation...
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Flow injection analysis of some oxidants using spectrophotometric detection
... evaluation of the obtained results, for the calibration graphs and for the analysis of some authentic samples, proves that this proposed method is reasonably accurate, precise, simple and cheap Flow ... The statistical evaluation gave a best straight line with a slope of 1.91, an intercept of 0.128 and a correlation coefficient of 0.998 The results of the analysis of some authentic samples (Table ... coefficient of 0.996 The results of the analysis of some authentic samples of hydrogen peroxide are shown in Table and in Fig The found results agree reasonably will with those expected showing an...
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Báo cáo khoa học: Epitope analysis of the rat dipeptidyl peptidase IV monoclonal antibody 6A3 that blocks pericellular fibronectin-mediated cancer cell adhesion pot
... (control) with horseradish peroxidase-conjugated streptavidin Immunofluorescent staining and fluorescenceactivated cell sorting (FACS) analysis rDPP IV -expressing HEK293 cells were stained with lgÆmL)1 ... or typeset Technical support issues arising from supporting information (other than missing files) should be addressed to the authors FEBS Journal 276 (2009) 6548–6559 ª 2009 The Authors Journal ... Bruce for his professional editing on our manuscript and Dr Ming-Derg Lai for his thoughtful discussion regarding the manuscript The authors also thank Ms Megan Cheng for her language assistance...
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báo cáo hóa học:" Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients" doc
... inter-assay performance assessment) Precision The precision of an analytical method describes the closeness of agreement (degree of scatter) between a series of measurements obtained from multiple sampling ... events) and the ELISPOT sensitivity was at 1/ 10,000–20,000 (using high PBMC assay), similar to data reported by others [1] For ELISPOT, assessment of assay sensitivity depends on number of TIL ... Flu, or PHA) – IFNγ secreting cells (stimulated with HIV as negative control) Statistical analysis Tetramer flow cytometric analysis was performed using Cell Quest Pro software (BD Biosciences)...
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Báo cáo hóa học: " Comparative transcriptomic profile analysis of fed-batch cultures expressing different recombinant proteins in Escherichia coli" doc
... calculated using statistical expression algorithm and average normalization was performed Hybridization and spike controls were used Subsequent data analysis was performed using GeneSpring GX11.5 software ... ’ara’ based expression of GFP were analysed to understand the host response towards hyperexpression of proteins (Figure 4, Additional file 4) The processes of cell growth and expression of foreign ... glyoxylate shunt (GS), respiration, transport, anabolism, catabolism and macromolecular degradation, protein biosynthesis, cell division, stress response, flagellar and chemotaxis system This coordinated...
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Báo cáo khoa học: "Flow cytometric evaluation on the age-dependent changes of testicular DNA contents in rats" pdf
... age of 26 weeks Discussion Several variables including seminiferous tubule diameter, testicular biopsy score, and tubular fertility have been used to assess the status of the testis Recently, flow ... differences in animal strains, tools for observation, and treatment processes for analysis In this study, the kinetics of cellular changes in PIstained testicular cells of growing SD rats was characterized ... Because of the heterogeneity of cell classes involved in spermatogenesis, a detail assessment of changes in cell cycle may not be possible by means of DNA frequency distribution patterns alone...
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Báo cáo y học: " The intracellular detection of MIP-1beta enhances the capacity to detect IFN-gamma mediated HIV-1-specific CD8 T-cell responses in a flow cytometric setting pro" ppsx
... determine positive responses (see Methods) To facilitate the comparison with the ELISPOT, ICS results were expressed as the sum of the CD8 and CD4 responses and a response in ICS was considered positive ... The ELISPOT detected 50 positive responses in 67 samples, while the ICS positive responses expressed as IFN-γ+ MIP-1β+ CD8 or CD4 T-cells were 55 and the ICS positive responses expressed as total ... proteins Correlation analysis of the responses measured by ELISPOT and by ICS expressed in terms of IFN-γ+ MIP-1β+ CD8 T-cells or total IFN-γ+ CD8 T-cells demonstrated in both cases a significant correlation...
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Báo cáo khoa học: " In vitro analysis of expression vectors for DNA vaccination of horses: the effect of a Kozak sequence" docx
... Svansson V, Olafsdottir G, Eiriksdottir FS, Jörundsson E, Arnadottir H, Marti E, Torsteinsdottir S: Human serum albumin model for comarison of expression vectors for DNA vaccination of horses ... Despite of containing Intron A the W1 and V1 vectors show less expression than G and H1 in all the cells This is presumably because of the lack of a Kozak sequence, as W2 and V2 vectors that have ... have a significant expression in horse cells we investigated the effects of Kozak consensus and intron A sequences on the levels of expression of the HSA gene Sequences flanking the AUG initiation...
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Báo cáo y học: " Analysis of novel geometry-independent method for dialysis access pressure-flow monitoring" pdf
... dialysis access is the ΔP between sensors (ΔPAV) Since PV, as it is used in dialysis access monitoring currently, is the relative pressure between the venous needle site and atmospheric pressure, ... measured), access diameter (or potentially more complicated forms expressing dialysis access geometry), and factors affecting fluid flow such as blood viscosity With any of these relationships, ... (EDM) has been used for three-dimensional machining of cavities in needle shafts for MEMS sensor integration within needles.[22] The possibility of using this type of approach is also supported...
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Báo cáo y học: "Statistical methods and software for the analysis of highthroughput reverse genetic assays using flow cytometry readouts" pps
... changes in the FSC or SSC signal A rough pre-selection using some fixed FSC and SSC threshold values, as provided by most FACS instruments, further increases robustness To see how this affects the ... artifactual correlation due to autofluorescence is absorbed by β The parameter α absorbs baseline fluorescence, as discussed below interactions Figure Selection of well measured cells Selection of well ... types of responses supports the use of regression analysis Because the effect may deviate from linearity in the range of perturbations that we observe, we use a robust local regression fit: OR...
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Báo cáo sinh học: "Immunological analysis of a Lactococcus lactis-based DNA vaccine expressing HIV gp120" ppt
... four vectors This IFN-γ induction was, however, lower for the L lactis based vectors than for the corresponding E colibased vectors Discussion In vitro stimulation of splenocytes by plasmid DNA ... pEC120CpG For both types of vectors the amounts of gp120 expressed seemed somewhat lower when they harboured the CpG cassette The L lactis based vectors drive therefore antigen synthesis as efficiently ... Innovation References Conclusion By this study we suggest an alternative host vector system for use as plasmid DNA vaccines The L lactis system is based on an endotoxin-free and safe organism and the...
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Báo cáo sinh học: "Comparative analysis of HIV-1-based lentiviral vectors bearing lyssavirus glycoproteins for neuronal gene transfer" pdf
... SH-SY5Y cells using lentiviral vectors pseuTransduction of SH-SY5Y cells using lentiviral vectors pseudotyped with different lyssavirus glycoproteins SH-SY5Y neuroblastoma cells were transduced ... lentiviral vectors in E15 rat mixed spinal cord cultures The vectors were then tested in mixed spinal cord cultures to assess their potential specificity for neurons as opposed to glial cells Cultures ... DRGs, and lumbar spinal cords were removed for analysis Sections were stained using X-Gal to visualize β-Gal expressing cells While all the vectors mediated some kind of retrograde transport...
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Báo cáo sinh học: "Comparative analysis of macrophage associated vectors for use in genetic vaccine" ppt
... using SPSS version 11 software Results Selection of promoter Following promoters were selected for the studies based on their known expression profiles Macrosialin is a glycoprotein expressed specifically ... activity of promoters under study, fluorescent microscopic analysis of GFP expressing cells were carried out Fluorescence of GFP increased based on the expression which correlates to the activity of ... Preliminary screening was performed using fluorescent microscope, green fluorescence was observed in cells transfected with respective constructs, confirming the successful protein expression Precise specificity...
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ANALYSIS OF INTEGRATION SITES OF TRANSGENIC SHEEP GENERATED BY LENTIVIRAL VECTORS USING NEXT-GENERATION SEQUENCING TECHNOLOGY
... large number of transgenic animal models have been successfully established to study mechanisms of human diseases in terms of gene-disease relationships, to evaluate gene therapy strategies, and to ... Lentivirus is one of subfamilies of retrovirus The first isolated lentivirus was equine infectious anemia virus (EIAV) Other lentiviruses were subsequently isolated from other species, such as feline ... sites in different tissues of each animal 498-1 Kidney Skin Placenta Intestine 714-1 Lung Kidney Skin 709-1 Kidney Placenta Inter uterus Skin Hyp IS IS1 IS2 IS1 IS2 IS1 IS2 IS1 IS2 # of reads...
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An exploratory analysis of the order book, and order flow and execution on the Saudi stockmarket
... negative sign on the aggressiveness indicator implies Table Survival analysis resultsaYb Buy orders Number of observations Number of observations Censored observations Sell orders 80257 39.21% 89987 ... This table presents statistics on tick sizes on the SSM The statistics are computed for all 56 stocks in the sample and for ®ve sub-samples classi®ed by the mean of stock price during the sample ... other columns report the cross-sectional distribution of these statistics across the 56 stocks in the sample All the reported statistics are mean values except for the number of observations and the...
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Analysis of transport phenomena and electrochemical reactions in a micro PEM fuel cell with nature inspired flow field design
... accounts for detailed species mass transport, heat transfer in the solids as well as in the gases, potential losses in the gas diffusion layers and membrane, electrochemical kinetics, and the transport ... condensation m phase < is assumed, so that the mass balance equations for both ( ) ( ) phases are; ∇ ⋅ ((1 − sat )ρ g εu g ) = m phase ∇ ⋅ (sat.ρ l εu l ) = m phase where sat is saturation, ε is porosity ... is the same as the gas phase temperature 2.2.2 Gas diffusion layers The physics of multiple phases through a porous medium is further complicated here with phase change and the sources and sinks...
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material flow analysis of phosphorous and chemical oxygen demand in domestic wastewater and food waste in song cong town, vietnam
... HSSF Horizontal subsurface flow MFA Material flow analysis N Nitrogen NGO Non-governmental organization P Phosphorus PAO Phosphorus accumulating organisms SBR Sequencing batch reactor SSF Subsurface ... tool for such planning is material flow analysis (MFA) This thesis uses MFA to define the current flows of phosphorus (P) and organic matter, measured as COD, in domestic wastewater and food waste ... results with a business-as-usual (BAU) scenario 1.4 Limitation of the research This thesis focuses on analyzing the flows of P and COD in wastewater and food waste from the households of the six...
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Static and Dynamic Analysis of Space frames
... Pre-processor for suspension bridges in structure-special Compensation of element length macro in structure-special Demonstration of new postprocessor look (TDF) in structure-special New chapter for ... error corrections in import/export with non-standard units LIST:GEN extension for selective result list of load cases, superposition files and influence lines 3.64 Version 8.38.03 • • • • Use of ... result superposition file) added Quick help for buttons and symbols integrated Reorganisation of graphic interface, separate menu for SCRIPTS Easy access to numeric result post-processing (former...
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