figure 1 schematic representation of different mechanisms by which nanocarriers can deliver drugs to tumours polymeric nanoparticles are shown as representative nanocarriers circles passive tissue targeting is achieved by extravasation of nanoparticles
báo cáo khoa học: "Immobilization of different biomolecules by atomic force microscopy" pptx
... level by self-assembly [13,14] is the basis for new technical applications Immobilization of DNA-molecules was tested as follows To prevent unspecific binding of DNA, the targeted surface was blocked ... feature of the method is that optimization of the spotting protocol can be reduced to just one spotting substance, regardless of what kind of biotinylated mixture of biomolecules is to be arranged ... biomolecules is to avoid any cross contaminations This can be achieved by either cleaning or replacing the tip However, replacing would result in a decreased accuracy due to long distance-moving of the...
- 7
- 285
- 0
Báo cáo khoa học: "Epstein-Barr Nuclear Antigen 1 modulates replication of oriP-plasmids by impeding replication and transcription fork migration through the family of repeat" ppt
... luciferase transcripts formed as a consequence of DBD bound to EBNA1 binding sites functioning as a transcription fork-block To test this hypothesis, the distribution of luciferase RNA in total ... decreases the replication potential of plas- mids, by reducing the frequency of replication initiation as DS, as the number of EBNA1-binding sites in FR is increased An alternative model is suggested ... the replication of oriPplasmids by acting in cis In this study, we have examined the mechanism by which the wild-type number of binding sites limits the replication of oriP-plasmids Our results...
- 15
- 212
- 0
Báo cáo khoa học: Inactivation of phosphorylase is a major component of the mechanism by which insulin stimulates hepatic glycogen synthesis doc
... phosphatase This reverses the inhibition of synthase phosphatase by phosphorylase a There is a long-standing debate as to whether inactivation of phosphorylase is a component of the mechanism by which ... (activation) of glycogen synthase (GS-b) by synthase phosphatase (SP) is inhibited by phosphorylase a (Phos-a) Conversion of phosphorylase a into phosphorylase b (Phos-b) by phosphorylase phosphatase ... (PP) is stimulated by glucose and by CP-91149 Insulin may convert phosphorylase a into phosphorylase b by either inhibition of phosphorylase kinase (PK) or activation of phosphorylase phosphatase...
- 9
- 381
- 0
báo cáo hóa học:" Outcome of Different Nevirapine Administration Strategies in Preventin g Mother-to-Child Transmission (PMTCT) Programs in Tanzania and Uganda" docx
... NVP-based PMTCT programs in rural areas of Tanzania and Uganda GTZ works in close cooperation with the Ministries of Health of the partner countries, and the programs are integrated into the existing ... order to have the NVP syrup administered to her infant by health personnel (semi-observed strategy) In both countries NVP is administered to the infant within 72 hours after birth In cases in which ... visit of the women, at delivery, and at each follow-up visit Due to logistic constraints, these data could only be collected from July to December 2003 During this period, the data were consist-...
- 8
- 335
- 0
báo cáo khoa học: "The mechanisms by which polyamines accelerate tumor spread" pdf
... anti-tumor immune function of the host during this process (5-d) In this section, the mechanism of cancer metastasis and the involvement of polyamines are discussed 5-a Separation of cancer cells from ... involved should be taken into consideration Mechanism of metastasis and involvement of polyamines (Figure 2) There are several steps that occur during metastasis: separation of cancer cells from the ... factor that directly influences the prognosis of patients with malignant disease is the capability of cancer cells to invade surrounding tissues and organs and evade immune cell defenses to metastasize...
- 9
- 243
- 0
báo cáo khoa học: " Cloning of transgenic tobacco BY-2 cells; an efficient method to analyse and reduce high natural heterogeneity of transgene expression" docx
... Transformation of BY- 2 line was performed by a slightly modified protocol introduced by [5] A ml aliquot of 3day old BY- 2 cells was co-cultivated with 200 μl of an overnight culture of Agrobacterium ... cell division by activation of its associated kinase Plant J 2007, 52:435-448 Kuthanova A, Fischer L, Nick P, Opatrny Z: Cell cycle phase-specific death response of tobacco BY- 2cell line to cadmium ... their laboratory We are also grateful to Prof Zdenek Opatrny (Faculty of Science, Charles University in Prague) for general support This work was supported by grants from the Ministry of Education,...
- 11
- 302
- 0
Báo cáo y học: "Creation and disruption of protein features by alternative splicing a novel mechanism to modulate function" docx
... CF147426 Acceptor upstream of exon Disruption of the second TM domain In general, any EST-based approach is hampered by the bias of publicly available EST databases towards cancer-related tissues or ... seven of the 20 examples, only one splice form is represented by a single EST or by cancerrelated ESTs However, the remaining examples are supported by several ESTs as well as ESTs from normal tissue, ... to an insert of 26 amino acids into a caspase-3 cleavage site and to an isoform that is caspase-insensitive [28] We have not investigated such features here since only a fraction of them have...
- 8
- 252
- 0
Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx
... medium of the D-form or the peptides was proposed as the reason of this apparent increased uptake, as the rate of uptake was the same in serum-free medium [17] As already stated above, this Tat ... linked b-galactosidase despite the higher number of peptides As far as Tat peptides are concerned, the 13-amino-acid peptide encompassing the basic domain of Tat (Tat 48±60) was found to be more ... domain of these proteins [6] Cellular internalization of this peptide fused to b-galactosidase was even observed in vivo in various tissues including the brain after intraperitoneal injection into...
- 8
- 485
- 0
Báo cáo khoa học: Two L-amino acid oxidase isoenzymes from Russell’s viper (Daboia russelli russelli) venom with different mechanisms of inhibition by substrate analogs pdf
... features of the dissociated cofactor were similar to those of FAD The shifting of absorption maxima of the enzyme-bound avin was due to its microenvironment The RP-HPLC proles of the cofactor dissociated ... localization of high concentrations of hydrogen peroxide The mode of hydrogen peroxide delivery has been suggested to be an important factor for efcient and tissue- specic induction of apoptosis [7,10] ... glycosydic part, as has been reported in the case of Ca rhodostoma LAAO [23], might be the cause of the chromatofocusing features for both the isoforms Molecular mass Puried L1 and L2 appeared in SDS...
- 18
- 306
- 0
Tài liệu Báo cáo khoa học: The Ets transcription factor ESE-1 mediates induction of the COX-2 gene by LPS in monocytes doc
... this process This factor is a likely regulator of COX-2 expression, as we recently discovered that ESE-1 plays an important role in the responses of various cell types to inflammatory mediators ... sites leads to a more drastic abolition of this induction It has also been shown that the pattern of expression of PEA3 in breast cancer samples correlates with the patterns of expression of HER-2 ... This result does not appear to be due to a higher production of the ESE-1 protein Western blot analysis of ESE-1 and PEA3 expression after transfection of equal amounts of expression vector into...
- 12
- 519
- 0
Báo cáo khoa học: Distinguishing between different pathways of bilayer disruption by the related antimicrobial peptides cecropin B, B1 and B3 pptx
... disruption Initially the peptide appeared to bind to the liposome, as indicated by the short period of mass increase, and binding was followed by mass loss, indicating liposome destruction This ... Ôcarpet-likeÕ action) of peptides such as cecropin P1 [28–32] However, it is not currently possible to assess the likely mechanism of membrane disruption of a given peptide simply on the basis of its structure ... functions by a markedly different mechanism from that used by CB and CB1 In summary, on the basis of liposomal lysis as a function of time observed by the change in SPR, the thermotrophic phase transition...
- 10
- 443
- 0
Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt
... close to 100 for [Ala9]SP (Table 1) This apparent discrepancy between the varying affinities and the nonvarying response of these agonists could be explained by the number of receptors to be occupied ... be occupied by these agonists to get activation of the second messenger cascade as reported [40] Enzymatic degradation of SP analogues ACE is a dipeptidyl carboxypeptidase known to hydrolyze ... vein, two NK-2 receptor bioassays [43] More recently, the same Ala substitution was reported to cause a significant decrease in biological activities of [Ala8]NKA measured in human tissues [44] Indeed,...
- 11
- 860
- 0
Báo cáo Y học: Mechanism of 1,4-dehydrogenation catalyzed by a fatty acid (1,4)-desaturase of Calendula officinalis pptx
... analysis GC-MS analysis of yeast lipids was performed using a Fisons VG TRIO 2000 mass spectrometer (VG Analytical, UK) controlled by Masslynx version 2.0 software, coupled to a GC 8000 Series gas ... conjugase, the yeast strain DTY10-a2 (MATa, fas2D::LEU2, can1 100, ura3-1, ade2-1, his3-11, his3-15) [28] was transformed with a plasmid (pYJ) comprised of the pYES2 vector Fig Generic mechanistic scheme ... Use of deuterium kinetic isotope effects to probe the cryptoregiochemistry of D9 desaturation J Am Chem Soc 118, 6295–6296 14 Pinilla, A., Camps, F & Fabrias, G (1999) Cryptoregiochemistry of the...
- 6
- 341
- 0
Báo cáo Y học: Regulation of glypican-1, syndecan-1 and syndecan-4 mRNAs expression by follicle-stimulating hormone, cAMP increase and calcium influx during rat Sertoli cell development pptx
... verapamil were purchased from Sigma (Saint-Quentin Fallavier, France) oFSH is a generous gift of National institute of Arthritis Metabolic and Digestive Diseases Avian Myeloblastosis Virus (AMV) reaction ... was obtained from 100 ngÆmL)1 of FSH corresponding to a 45% decrease in the glypican-1 mRNA expression (Fig 1) Glypican-1 and syndecans-1 and -4 mRNA expression are regulated by the increase of ... treatment is expressed vs control which is arbitrarily set to 100% by this second messenger Moreover, syndecan-4 mRNA expression was stimulated by intracellular cAMP increase in 30-day-old rat Sertoli...
- 9
- 342
- 0
Báo cáo khoa học: Slow deactivation of ribulose 1,5-bisphosphate carboxylase/oxygenase elucidated by mathematical models pdf
... are aware of are provided by Kim and Portis [10] in a study conducted with RuBisCO isolated from spinach There, no effect of oxygen on the fallover extent was observed This can be explained by ... whereas the dashed lines denote the response of the observed fallover rate Similar to the case of the inhibitor XuBP, increasing the production rate of the inhibitor here also leads to an increased ... quantities are derived, allowing for an easy t of parameters to various types of RuBisCO This allows the identication of key features determining the distinct kinetic behaviors of different RuBisCOs...
- 20
- 247
- 0
Báo cáo khoa học: Mechanisms of cholinesterase inhibition by inorganic mercury potx
... cholinesterases to mercury Thus, it is a prerequisite to investigate the mechanism(s) of inhibition and to ascertain the reliability of using cholinesterases Cholinesterases are believed to be sensitive ... highly polluted areas The initial objective of this study was to evaluate the inhibition of cholinesterases by mercury, with a view to using them as biomarkers to survey polluted areas or for incorporation ... mechanism of inhibition A goal of this study was to address the issue of a clearcut model for cholinesterase inhibition by mercury It appears that two different mechanisms for cholinesterase inhibition...
- 13
- 350
- 0
Báo cáo khoa học: The enzymatic activity of SR protein kinases 1 and 1a is negatively affected by interaction with scaffold attachment factors B1 and 2 pot
... function of SAFB1, however, is transcriptional repression It was initially shown that SAFB1 binds to, and acts as, a corepressor of estrogen receptor a [39] It was further shown that it is capable of ... nuclear receptors [40] This repression might be effectuated, in some cases at least, by its interaction with the nuclear receptor corepressor which facilitates binding of histone deacetylases to the ... anti-GFP IgG was used as control (lane 3) A standard amount of cell extract, one-fifth of which is shown (lane 2), was used in each immunoprecipitation assay Molecular mass markers are shown in kDa...
- 16
- 573
- 0
Báo cáo Y học: Interaction of plasminogen activator inhibitor type-1 (PAI-1) with vitronectin Characterization of different PAI-1 mutants pdf
... charge of this region signi®cantly enough to reduce af®nity to Hep Surprisingly, the inhibitory activity of A114±118 towards uPA was reduced in a dose dependent manner by addition of Hep This may ... tumor invasion and metastasis Whereas tPA, at least in breast and ovarian cancer, does not play a major role in tumor cell invasion, uPA is an important, multifunctional component of the invasion ... not display a signi®cantly altered af®nity to Hep in SPR, although it was not able to inhibit thrombin as ef®ciently as wild-type PAI-1 together with Hep This contrasts with the results of Sui...
- 9
- 295
- 0
Báo cáo sinh học: " Inhibition of human immunodeficiency virus type-1 (HIV-1) glycoprotein-mediated cell-cell fusion by immunor (IM28)" docx
... was determined as described in Table of 22 mM and the CC50 as determined on MT2 cells was 75 mM giving a selectivity index of 3.4 Discussion IM28 is a potent new derivative of DHEA that can stop ... presence of dexamethasone (B) cells are mainly exploded vs in the presence of IM28 Figure dexamethasone on fusion of TF228.1.16 cells with SupT1 cells Effect of2 Effect of dexamethasone on fusion of ... other factors that affect viral tropism are present HIV-1 viral entry is inhibited in the presence of the ligands to these chemokine receptors RANTES, MIP-1α and MIP-1β, all of which are ligands...
- 6
- 382
- 0
Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu