... bacteriological laboratory, so far as the glass apparatus is concerned, differs but little from that ofa chemical laboratory, and the cleanliness ofthe apparatus is equally important The glassware comprised ... 21).—These are used forthe collection and analysis ofthe gases liberated fromthe media during the growth of some varieties of bacteria and may be either plain (a) or graduated (b) A simple form ... Hot air (for the sterilisation of all glassware and of metal apparatus) (b) Moist Heat: Water at 56° C (for the sterilisation of certain albuminous fluids) Water at 100° C (for the sterilisation...
... frequency of their representation in the stand composition) from each taxon used forthe reforestation ofthe state of reclamation A and “B” Fig Tilia cordata Mill and Pinus nigra Arn in the treatment ... Available nutrients (mg/kg) Ca were evaluated One-factor ANOVA was used forthe analysis ofthe growth data With respect to the applied amendment (CCB) the evaluation ofthe growth vitality of ... on the basis of collections and analyses of leaf (needle) samples of different tree species taxa fromthe assessed state of reclamation A and “B” The methodical procedure ofthe analysis of...
... [http://www.cbo.nl/product/richtlijnen/ handleiding_ebro/handl_totaal.pdf?] Collaboration AGREE: Development and validation of an international appraisal instrument for assessing the quality of clinical practice guidelines: the AGREE ... formulation ofa search strategy for locating relevant literature; critical appraisal of selected literature; and phrasing of recommendations for clinical practice [4-7] Quality criteria forthe development ... that may be relevant forthe critical appraisal of research publications Participants are divided into subgroups and given an assignment in which they must apply the tool by assessing the abstracts...
... [http://www.cbo.nl/product/richtlijnen/ handleiding_ebro/handl_totaal.pdf?] Collaboration AGREE: Development and validation of an international appraisal instrument for assessing the quality of clinical practice guidelines: the AGREE ... formulation ofa search strategy for locating relevant literature; critical appraisal of selected literature; and phrasing of recommendations for clinical practice [4-7] Quality criteria forthe development ... that may be relevant forthe critical appraisal of research publications Participants are divided into subgroups and given an assignment in which they must apply the tool by assessing the abstracts...
... design and coordination, and oversaw the data analysis and drafting ofthe manuscript PFS and DC designed and carried out the in vivo arthritis studies All authors read and approved the final manuscript ... transcript sequences from available sequence databases, including Geneseq and other public databases Table Cloned alternative splice variant mRNAs Detection of alternative splice variant mRNA ... 4c) Evaluation of alternative splice variant activity in an in vivo model of arthritis Since angiogenesis plays a key role in RA, we next evaluated the therapeutic potentialof ASV in an extensively...
... prediction and the available transcriptome data, can be applied forthe initial detection and isolation of NRPs [20] Furthermore, this approach substitutes the CAS assay-guided fractionation and enabled ... residue pattern, leading to the assumption that both activate the same substrate (Table S 2A) On the basis ofthe bioinformatic analysis ofthe etc gene cluster, it was predicted that the assembled ... identified on an analytical scale The sensitivity of radioactivity detection and sophisticated analytical separation proved to be advantageous in this approach The iron-chelating properties ofthe FEBS...
... (in Japanese) IPCS (1989) Environmental Health Criteria 89 Formaldehyde, World Health Organization, Geneva Takeda, M., Saijo, Y., Yuasa, M., Kanazawa, A. , Araki, A and Kishi, R (2009) Relationship ... Simultaneously, a new standard was added to the Japanese Industrial Standard (JIS) that the method of measuring the chemical emission rate from architectural materials should be divided grades.151) ... metropolitan areas complained of narrowness of their houses in the 1960 s At the time, airtight houses started to be built in urban area, and air pollution due to a lack of fresh air because of the...
... more adequate for molecular evolutionary analysis ofthe DNase I family The mammalian group formed a relatively tight cluster, while the snake (E quadrivirgata, E climacophora and A blomhoffii), amphibian ... obtained fromthe Japan Snake Institute, Gunma, Japan Phenyl Sepharose CL-4B, DEAE Sepharose CL-6B and Superdex 75 were purchased from Amersham Pharmacia Biotech; Concanavalin A (Con A) agarose ... Total RNA was isolated from each snake pancreas by the acid guanidinium thiocyanate/phenol/chloroform method [24] and any DNA contamination was removed by treatment with RNase-free DNase I (Stratagene)...
... Preparation of crab sera Freshwater field crabs, Paratelphusa jacquemontii were collected fromthe local wetlands of Kanyakumari district, India The crabs used for experimental purpose were of either ... by affinity chromatography It yielded a 2000-fold increase in specific activity Analysis ofthe lectin on SDS/PAGE gave a single band at apparent molecular mass of 34 kDa The binding affinity ofthe ... Denmark 47 Kamiya, H & Ogata, K (1982) Hemagglutinins in the acorn barnacle Balanus (Megabalanus roseus): purification and characterization Nippon Suisan Gokkaishi 48, 1427 48 Kamiya, H., Muramoto,...
... makes the worm a chrysalis, and the chrysalis a butterfly, and the butterfly dust? And is it sin, which makes the child a man, and the man a gray-haired man, and the gray-haired man dust? And what ... landscape on either bank seems to change But then come the cataracts of life They are firmly fixed in memory, and even when we are past them and far away, and draw nearer and nearer to the silent ... hearts gradually and irresistibly, as they won the hearts ofthe apostles and early Christians, confront us fromthe earliest childhood as the infallible law ofa mighty church, and demand of...
... because it may form the basis ofa drug acting in pathological situations involving the overexpression of TNF -a Materials and methods Preparation of rIris, mutant L33 9A and the cleaved forms of ... placed in glass test tubes Radioactivity was measured using a gamma counter (LKB, Wallac, Finland) All animals were maintained and handled according to local and national ethical guidelines Animal ... cytokine analysis (MCP-1, TNF -a, IL-6, IL-10, IL-1b) All animals were maintained and handled according to local and national ethical guidelines Statistical analysis Data are represented as means ±...
... synthesized from poly (A) + mRNA using M-MLV reverse transcriptase The cDNA was joined with the adaptor provided by the kit (5¢-gcugauggcgaugaaugaacacugcguuugCUGG CUUUGAUGAAA-3¢) using T4 DNA ligase ... spectra produced were analyzed manually and automatically by SEQUEST software The acquisition and deconvolution of data were performed with the XCALIBUR software on a Windows NT PC data system ... were obtained from data bank and the abbreviations stand for: AaH, Androctonus australis Hector; Amm, Androctonus mauretanicus mauretanicus; Bj, Buthotus judaicus; Bot, Buthus occitanus tunetanus;...
... through language use Grabe and Kaplan [5] also emphasize that only teachers who understand theory and make a transition from theory to practice can make the most appropriate decisions fora successful ... first language Many EFL teachers in Vietnam also regard this as a vital reason when analyzing their learners’ errors Nonetheless, as Savignon [15] puts it, the analysis of learners’ errors by means ... in an insightful manner (Bardovi-Harlig, [1]) In the examples, (1) Mary is talking to a man The man is Mary’s father, and (2) The man who Mary is talking to him is her father, we can see that...
... Rha, which could be obtained as a result ofthe incomplete substitution with ara4dHex or partial cleavage of terminal ara4dHex during isolation ofthe OPS by mild acid degradation ofthe LPS The ... GLC-MS ofthe partially methylated alditol acetates (Table 1) revealed terminal ara4dHex, 3-substituted Fuc and 3,4-disubstituted Rha as the main constituents, as well as a small amount of 3-substituted ... above for sugar analysis, and the partially methylated monosaccharides derived were converted into the alditol acetates and analysed by GLC-MS Smith degradation OPS-I, OPS-II and the O-deacetylated...
... aspartate (Asp378 in PMA1_NEUCR), several cavities are also seen at the same position in ATC1_RABIT (data not shown) Aside from mutations directed against a small stretch of amino acids adjacent to the ... horizontal black lines delineate the approximate membrane position The catalytic Asp (Asp378) appears in red defective in the E1-E2 conformational change Here, the Val336Ala mutant displayed kinetic ... enzymes for which structural data are available MATERIALS AND METHODS Building the 3D model of PMA1_NEUCR In our approach, the model of PMA1_NEUCR was built using the ATC1_RABIT crystal structure as...
... interaction with their membranes Materials and methods Materials All chemicals were ofthe purest analytical grade available Proteases, aprotinin, lysozyme, and a- casein were from Sigma-Aldrich ... concentration, v0 was calculated for each concentration ofthe inhibitor by nonlinear t ofthe data fromthe above negative controls; [I] is the concentration ofthe inhibitor, IC50 is the concentration ... determination of whole blood and plasma Anal Biochem 93, 98 102 22 Nakamura, Y., Gojobori, T & Ikemura, T (2000) Codon usage tabulated from international DNA sequence databases: status forthe year 2000...
... any) of each utterance on the basis ofthe anaphoric information and according to the notion of ranking specified This information was the used to find violations of Constraint and Rule The behavior ... segmentation and about ranking We discuss some ofthe problems in what follows (The latest version ofthe annotation manual is available fromthe GNOME project’s home page.) Utterances Kameyama (1998) ... leaflet was treated as a separate segment We then identified by hand those violations of Constraint that appeared to be motivated by too broad a segmentation ofthe text.2 Automatic computation of...
... ignorance ofthe slaves as an argument against their emancipation, the antislavery men's problem became the education ofthe master as well as that ofthe slave Believing that intellectual and moral ... uplands of Pennsylvania and roamed over the hills ofthe Appalachian region as far south as Carolina A painting ofa group of their converts prior to 1747 shows among others two Negroes, Johannes ... some of them.[4] [Footnote 1: Davis was a logical antislavery agitator He believed that if the slaves had had the means of education, if they had been treated with humanity, making slaves of them...
... Chemistry, VAST, and at the herbarium ofthe College of Pharmacy, Chungnam National University, Korea Extraction and isolation Dried and powdered leaves (3.7 kg) were extracted repeatedly with ... hot-stage melting point apparatus IR spectra was obtained on a JASCO DIP-370 Digital polarimeter Optical rotations were determined on a JASCO DIP-1000 KUY polarimeter FAB-MS and HR FAB-MS were obtained ... internal standard Column chromatography (CC) was performed on silica gel 60 Plant material A trifoliatus was collected in Langson province, Vietnam in January 2001 and identified by Prof Dr Tran Minh...