extra telomeric roles of telomeric proteins
The roles of shca proteins in response to oxidative stress 1
... Phosphorylation of p66shcA on Ser36 was determined by Western blot analysis (D) Coexpression of the constitutively active MEK1 (MEK1 ) led to massive phosphorylation of p66shcA on Ser36 The mutant form of ... expression of p27 and its role in oxidative stress response (A) H2O2 treatment down-regulated expression of p27 without affecting MnSOD Cells were treated with 0.1–0.5 mM of H2O2 for 20 h The levels of ... Inhibition of ERKs was found to rescue down-regulation of p27 (B) RT-PCR experiments showed that H2O2 treatment down-regulated expression of p27 at mRNA levels Notice that the p27 is a negative image of...
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The roles of shca proteins in response to oxidative stress 2
... phosphorylated by PKC δ (B) Mass spectrometric analysis of phosphorylation sites on ShcA Tandem mass spectrum showing the sequence of a peptide of m/z 611.7891 which was eluted from the reversed phase ... associated proteins were precipitated with anti-PKC δ antibodies and p46shc was detected with anti-ShcA antibodies Note in A and B, p52 construct expressed both p52 and p46 Shc due to the use of two ... of two start codons Bottom panel: quantitation data from B The value of co-immunoprecipitated p52Shc or p46Shc in the absence of H2O2was set at -95- C IP: anti-HA (p52) IB: PKC δ H2O2 + - + p52Shc...
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Báo cáo khoa học: Identification of three proteins that associate in vitro with the Leishmania (Leishmania) amazonensis G-rich telomeric strand pdf
... fractions of S100 and nuclear extracts from L amazonensis promastigotes Due to the limiting amount of telomeric proteins present in these extracts, binding reactions were done with a minimum of lg of ... nuclear extracts were done in the presence of a standard concentration of KCl (100 mM) used in normal reactions and varying concentrations of MgCl2 (0–50 mM), or of a standard concentration of MgCl2 ... amazonensis proteins that bind in vitro to the telomeric G-strand (L amazonensis G-strand telomeric proteins; LaGT1, LaGT2 and LaGT3) Binding activities were found in S100 and nuclear extracts of L...
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Báo cáo khoa học: cGMP and glutathione-conjugate transport in human erythrocytes The roles of the multidrug resistance-associated proteins, MRP1, MRP4 and MRP5 pot
... numbers of fitting parameters were compared using an F-test on the ratio of the variance associated with the reduction in degrees of freedom to the variance of the fit with the smaller number of degrees ... smaller number of degrees of freedom [33], VR ¼ ðSSE2 À SSE1 Þ=ðd.f.2 À d.f.1 Þ SSE1 =d.f.1 ð5Þ where d.f is the number of degrees of freedom (¼ number of data points ) number of fitting parameters) ... glycoproteins [34] PNGase F hydrolyzes nearly all types of N-glycan chains from glycopeptides /proteins Results ATP-dependent uptake of cGMP into human erythrocyte membrane vesicles The rate of...
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Roles of tuberous sclerosis complex proteins in autophagy and cell death
... objective of this study is to investigate the roles of TSC proteins in cell death by focusing on the involvement of autophagy and JNK signaling pathway, with the following aims: 1) elucidation of the ... here iii TABLE OF CONTENTS ACKNOWLEDGEMENTS II TABLE OF CONTENTS IV SUMMARY IX LIST OF TABLES XI LIST OF FIGURES XII LIST OF ABBREVIATIONS ... elucidation of the role of TSC to autophagy in response to starvation and 2) examination of the role of TSC in oxidative stress-mediated JNK signaling In the first part of our study, we found...
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Study of the associated proteins of STAT3 and characterization of their functions roles of GRIM 19 and PIN1 in the regulation of STAT3 activity
... STUDY OF THE ASSOCIATED PROTEINS OF STAT3 AND CHARACTERIZATION OF THEIR FUNCTIONS: ROLES OF GRIM-19 AND PIN1 IN THE REGULATION OF STAT3 ACTIVITY LUFEI CHENGCHEN B ... other areas of negative regulation of STATs, 24 Chapter 1: Introduction physical degradation of STAT proteins is less understood Evidence of ubiquitin-proteasome-mediated degradation of STAT protein ... receptor binding activity of Stat3 proteins (Zhang et al, 2002) Roles of the coiled-coil domain in STAT nuclear translocation were brought into attention by the identification of several nuclear export...
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Immunomodulatory roles of heat shock proteins
... in the absence of OVA (No), or in the presence of 50 µg/mL OVA (ova), 50 µg/mL of OVA plus 5-200 µg/mL of respective heat shock proteins (Hsp/ova), 50 µg/mL of OVA plus 100 µg/mL of lactorferrin ... absence of OVA (No), or in the presence of 50 µg/mL OVA (ova), 50 µg/mL of OVA plus 5-200 µg/mL of respective heat shock proteins and 20 µg/mL PMB(Hsp/ova) , 50 µg/mL of OVA and 100 µg/mL of lactorferrin ... the absence of modulator (A, stained ctrl), or in the presence of 100 µg/mL of Lactoferrin (A, Lacto), µg/mL of lipopolysaccharide (B, LPS), 100 µg/mL of Hsp70 (C, Hsp70), 100 µg/mL of Hsp60 (D,...
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Use of Cyanobacterial Proteins to Engineer New Crops
... biofarming is a major goal of plant biotechnology Direct transfer of plant genes has resulted in new varieties with improved properties However, this approach is limited by the genetic stock of ... the various existing Use of Cyanobacterial Proteins 67 Fig 4.1 Summary of current advances in plant biotechnology using cyanobacterial genes Gene mining on cyanobacteria offers a promising opportunity ... phytochrome reaction spectra Production of biodegradable polymers (Section 4.5) Phytochrome perception (Section 4.6) (synthesis of PCB) Biofarming Production of cyanophycin Pigment manipulation (Section...
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Tài liệu Roles of CIO for Innovative Government ppt
... IT professionals The changing roles of CIOs The changing roles of CIOs In the 1980s - CIOs are chief information officers who manage information systems and information distribution in offices ... chief of division other deputy mayor chief of a section ４ Social Cognition of CIO IT Revolution (Maximizing the effectiveness of IT investment) New business models Issues ： ◆HRD for CIO － Lack of ... of Administrative management Bureau, MIC, Member: CIO of all ministries Ministerial Committee of Directors in charge of e-Government Other InterMinisterial Meeting Inter-Ministerial Meeting of...
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Tài liệu Báo cáo khoa học: Seeking the determinants of the elusive functions of Sco proteins pptx
... divergence of Sco proteins in prokaryotes is apparent from the analysis of their genomes, Determinants of the elusive functions of Sco proteins some of which contain genes coding for Sco proteins ... variety of possible functions Given that the fold of Sco proteins resembles that of proteins involved in thiol-disulfide redox chemistry, it is reasonable to assume that the primary role of Sco proteins ... oxidoreductase function of the CXXXC motif of human Sco proteins could therefore be implicated not only in the maturation of the CuA site of Cox2 but also in the maintenance of cellular copper homeostasis...
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Tài liệu THE ROLES OF AMINO ACID CHELATES IN ANIMAL NUTRITION pdf
... components of soft tissue; fluorine, zinc, and silicon play roles in the formation of proteins and fats that compose the body (8) Minerals also preserve cellular integrity through their roles in ... the regulation of body processes.() The need for chromium to potentiate insulin and the involvement of phosphorus in the ATP mol ecul e are two exampl es of the di verse roles of minerals in ... retired and working as a consultant The Roles of Amino Acid Chelates in Animal Nutrition are an important part of protein and lipid fractions of As regul ators of body processes the animal body minerals...
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Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc
... genetic diseases [47] Most analyses of the regulation of AP-2 and the interactions of the transcription factor with binding partners, as well as of the regulation of target gene expression, have ... treatment of the chick face with RA, and this is accompanied by an increase in apoptosis [73] The authors of this study ascribe the regulation of outgrowth of limb buds and patterning of the digits ... Wenke and A.-K Bosserhoff growth retardation, AP-2s play vital roles in cell proliferation Besides the functions of AP-2s in physiological processes, they have also crucial roles in pathological...
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Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx
... basis of incorrect pairing of sister chromatids and of the lack of integrity of the synaptonemal complex in Hsf2) ⁄ ) spermatocytes is a pending question [62] Role of HSF1 in the quality control of ... spermatids (a stage of profound chromatin remodelling), and HSF2 is a transcriptional regulator of Ssty2, Sly and Slx, because the loss of HSF2 results in down-regulation of the levels of Ssty2 and ... possible roles of HSF2 in the organization of chromatin and of the genome structure First, HSF2 binding to its target genes correlates with the acetylation of histones H3 and H4, a frequent mark of...
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Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc
... expression level of the GFPtagged fusion proteins is indicative of that of the equivalent untagged proteins We examined whether the various charged-to-alanine substitutions affected the ability of full-length ... We expect that the relative expression level of the GFP-tagged proteins is indicative of that of the equivalent untagged proteins Lipid anchoring of C-Vrp1p bypasses the requirement for the LBD ... relative expression level of the tagged proteins is indicative of that of the equivalent untagged proteins To assess the function of these proteins in endocytosis we carried out LY uptake assays on...
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Tài liệu Báo cáo khoa học: Roles of the human Rad51 L1 and L2 loops in DNA binding doc
... eukaryotic and archaeal proteins (Fig 1A) Like the case of bacterial RecA, the L1 and L2 loops of the eukaryotic Rad51 proteins face A Roles of the HsRad51 L1 and L2 loops inside of their helical filaments, ... fluorescence changes of these proteins were almost saturated at bases per monomer of poly(dT), showing that the changes were mainly due to the binding of the first DNA, while the binding of the second ... Roles of the HsRad51 L1 and L2 loops D231W (L1 loop) Table Changes in the fluorescence of tryptophan probes inserted in the L1 and L2 loop upon binding of nucleotides and DNA The fluorescence of...
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Tài liệu Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control doc
... region of the Fe(III) species, spectra of the Asp40 mutants were essentially similar to that of the wild-type protein Additionally, spin states of the Fe(III), Fe(II) and Fe(II)-CO complexes of Asp40 ... structure of Ec DOS PAS [17] As the structure of the cyanide-bound complex of FixL is similar to that of its oxygen-bound complex [10], a cyanide binding study should facilitate elucidation of the ... component of a denatured form The redox state of haem is related to the PDE activity of Ec DOS, since it is a haem redox-sensing enzyme [2,3] Thus, it is important to determine the redox potentials of...
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Tài liệu Báo cáo khoa học: The social life of ribosomal proteins doc
... functional effects of these mutations were due to the architectural and stabilizing role of the proteins alone, i.e that perturbation of protein structure would affect the catalytic activity of the rRNA ... composed of RNA However, two long C-terminal tails of small ribosomal subunit proteins S9 and S13 that are otherwise located at the top of the 30S, make their way down through the head of the subunit ... antiterminator of transcription of k phage N protein in E coli [58] Furthermore, several ribosomal proteins are known to regulate the expression of themselves or other ribosomal proteins through...
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Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf
... proteins except for RTnI96)181 Inhibition of Mg-ATPase of actomyosin by TnI-fragments The inhibition of actomyosin-tropomyosin Mg-ATPase by TnI fragments was compared The inhibitory effects of ... binding of the C-terminal region of TnI were not present in Akazara scallop troponin Ca2+-regulatory effects of troponins containing TnI fragments The Ca2+-regulatory effects of troponins composed of ... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 41 Harafuji H & Ogawa Y (1980) Re-examination of the apparent binding constant of...
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Tài liệu Báo cáo khoa học: Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181 and His184 pdf
... analysis of the titration of H181A PETN reductase with progesterone (C) Plot of absorbance change as a function of 2,4 dinitrophenol concentration for the data shown in (A) (D) Plot of absorbance ... His-His pair, there has been no report of a systematic analysis of the contribution of each histidine residue to binding and catalysis Thus, to ascertain the role of each histidine residue, and to ... reductase Fig Analysis of ligand binding by equilibrium titration studies (A) UV-visible analysis of the titration of H181A PETN reductase with 2,4 dinitrophenol Inset, detail of the absorbance change...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose