experimental evidence for ph dependent changes in the structure or orientation of adsorbed proteins
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 11 ppsx
... and if the time dependence of the Amide II intensity is analyzed for varying pH, information on the pH dependence of the α-chymotrypsin orientation on the montmorillonite surface can be obtained ... area of the surface in contact with the protein and on the secondary structure of adsorbed proteins It has been shown that both pH- dependent modification of conformation and pH- dependent orientation ... observed for the decreases of the Amide II bands for both adsorbed and solution states Unfolding of bundled/internal helical domains increases water diffusion inside the core of the protein (Fig...
- 22
- 424
- 0
Tài liệu Báo cáo khoa học: Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of specificity, mechanism and mode of inhibition by analogues of high-energy reaction intermediates pdf
... On the other side, the adenine forms hydrophobic interactions with Ala78 and Ala79 The hydrogen bonding of the enzyme with the adenine part of the cofactor is predominantly with the 2¢-phosphate ... domains, here 6PG lies across a8, which forms the floor of the active site The C1 of 6PG is placed near to the cofactor-binding domain with the phosphate directed to the loop between a10–a11 and the ... coloured according to the domain in which they occur; blue for domain I, yellow for domain II and red for domain III Aligned sequences of OaPDH and TbPDH are also shown Most of the amino acids...
- 12
- 452
- 0
Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc
... to conformational changes in the substrate, thereby facilitating binding to the substrate-binding cleft It is obvious that elucidation of the 3D structure of PelB would give more insight into ... calculate the binding afnity for subsite )1 The kint value was found to be 262 s)1 Afnity values are shown in Fig as a schematic representation of the subsite binding cleft of PelB The highest binding ... Km, increased constantly with increasing DP, with a value for (GalpA)8 almost 30-fold higher than for (GalpA)2 (Table 1) Subsite mapping On the basis of the assumptions of Hiromi [30] that the intrinsic...
- 10
- 592
- 0
Báo cáo khoa học: Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus doc
... partly dependent on, and partly independent of, CagA tyrosine phosphorylation, and the phosphorylation motifs located in the C-terminal region of CagA (EPIYA motifs) are thus essential for some phenotypes ... supporting the idea that CagV might act as a nucleator by forming contacts with other core complex proteins A further step in the assembly of the secretion apparatus would be the recruitment of ... these proteins are necessary for inducing secretion of the chemokine interleukin (IL)-8 from infected epithelial cells [9,10] In the corresponding strain NCTC11638, the cagPAI is not a contiguous...
- 10
- 393
- 0
Báo cáo khoa học: Structural and thermodynamic insights into the binding mode of five novel inhibitors of lumazine synthase from Mycobacterium tuberculosis pptx
... shift in the position of the pyrimidine ring, whereas the phosphate and phosphonate moieties occupy the same position in spite of the different conformation of the alkyl chain The positions of the ... chain in the other complexes Those water molecules form the hydrogen bond network connecting the phosphate ion with the N7 atom of the aromatic purinetrione ring system Binding mode of the chloropyrimidine ... chain in the other structures The distance between the pyrimidine ring and the phosphate atom in the phosphate moiety is 6.9 A The location of this group is the same as in the structures of MbtLS...
- 15
- 439
- 0
Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
... This may be interpreted as the first evidence for a restricted velocity in the translocation step of the loaded transporter by the sterical conformation of the substrate The introduction of polar ... speculative, the rigid ring structure of proline may cause a different orientation of the substrate within the substrate binding site of PAT2, and this may simultaneously avoid the interference of the ... measured in the absence of inhibiton Data are means ± SEM (n ¼ 8–10) ND, not determined Elongation of the side chain glycine L-Alanine L-a-Aminobutyric acid L-Norvaline L-Norleucine Elongation of the...
- 8
- 541
- 0
Báo cáo khoa học: Exo-mode of action of cellobiohydrolase Cel48C from Paenibacillus sp. BP-23 A unique type of cellulase among Bacillales ppt
... cultures of recombinant E coli/pUCel48C, were mixed with an equal volume of a 5% suspension of Avicel in water Binding to Avicel was performed in batch for h at °C in 50 mM phosphate buffer pH 6.0, ... with the structural designation CD48/ Fn3/CBM_3a to indicate the type and location of the different domains and providing information about the organism of origin, Paenibacillus sp BP-23 [3,7] The ... cellobiohydrolases For this purpose, further synergism assays [5,11,33] are being performed using combinations of Cel48C and other endo- or exo-cellulases from either the same or different strains The biochemical...
- 7
- 404
- 0
Báo cáo khoa học: The twin-arginine translocation (Tat) systems from Bacillus subtilis display a conserved mode of complex organization and similar substrate recognition requirements doc
... for export We also tested the importance of the +2 leucine residue (Leu19) within the consensus motif by testing for export of the DmsA–GFP fusion carrying the L19A, L19D and L19F mutations In ... The protein has phosphodiesterase and alkaline phosphatase activity, and PhoD is targeted to the cell wall, where it is involved in the release of inorganic phosphate [20] The absence of a TatB ... the twin arginine motif, the )1 serine residue and the +2 leucine residue (with respect to the twin-arginine motif) are all important for efficient translocation by TatAdCd and E coli TatABC In...
- 12
- 445
- 0
Báo cáo khoa học: The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme doc
... are important for competent binding of the substrate Therefore, a calorimetric study was carried out to further investigate the importance of sulfur–sulfur interactions for the affinity of thiolase ... in detail below, indicate a crucial role for sulfur–sulfur interactions in defining the catalytically competent binding mode of CoA in the active site The pantetheine binding tunnel of biosynthetic ... atoms of ˚ SPP and the reactive Cys89 is 4.1 A, whereas the cor˚ responding distance for the CoA complex is 3.9 A (Table 2) The pantetheine moiety of SPP binds in the pantetheine binding tunnel in...
- 13
- 472
- 0
Báo cáo khoa học: Structures and mode of membrane interaction of a short a helical lytic peptide and its diastereomer determined by NMR, FTIR, and fluorescence spectroscopy pdf
... stretches within amino acids is probably a major driving force for membrane binding, compensating for the reduced a helical structure Furthermore, both the blue shift in tryptophan fluorescence observed ... resembling a wide turn is obtained for K4L3l4W in the SDS micelles (Fig 4D) Importantly, the positions of the leucine and lysine side-chains emphasize the amphipathic organization of the peptides The ... study of lipid–protein interactions in membrane models Biochim Biophys Acta 467, 1–11 44 Vogel, H (1981) Incorporation of melittin into phosphatidylcholine bilayers Study of binding and conformational...
- 12
- 409
- 0
Báo cáo khoa học: "Metaphor Comprehension - A special mode of language pptx
... number of important connections between the GCS and the TKS, it is necessary to search for additional knowledge structures which represent context information which was missing in the original input ... sLmulation metaphor comprehension Recursive metaphor interpretation - many of the elements of the GCS are themselves m e t a p ~ s in that they contain implicit semantic mismatches These metaphoric elements ... In addition, certain input metaphors require other comprehension strategies to be invoked, such as context construction or recursive metaphor interpretation COMPARISON WITH OTHER THE? RIES The...
- 2
- 311
- 0
Báo cáo khoa học: Peptides that bind the HIV-1 integrase and modulate its enzymatic activity – kinetic studies and mode of action pptx
... 401413 + + + + + IN- 1 a IN- 4 + + + + + + + + + + + a Table Summary of IN- binding domains for the various IN- interacting peptides Binding of the various IN- interacting peptides to an IN- derived peptide ... probably indicating enhancement of the ININ interaction However, an almost linear curve was observed in the presence of all of the inhibitory peptides (Fig 4), indicating eradication of the autocatalytic ... approaches for the design of anti-HIV therapy A promising approach is the use of peptides for inhibition or activation of certain viral or intracellular target proteins [21] New emerging technologies...
- 15
- 343
- 0
Báo cáo khoa học: Common mode of DNA binding to cold shock domains Crystal structure of hexathymidine bound to the domain-swapped form of a major cold shock protein from Bacillus caldolyticus pot
... stage The association of subdomains results in the formation of the closed b barrel burying DNA single-strand binding to the cold shock domain the hydrophobic core At present there is no experimental ... sugar– phosphate backbone shields the hydrophobic nucleobases and the hydrophobic-binding platform of the protein below them from the polar solvent (Fig 2B) The sugar of T1 from chain C maintains ... crystal structures can also be found in the sequences of Y-box proteins, with the exception of side chains forming nucleobasebinding subsites and (Fig 9B) We therefore expect that the Y-box proteins...
- 15
- 333
- 0
Báo cáo khoa học: Contribution of a central proline in model amphipathic a-helical peptides to self-association, interaction with phospholipids, and antimicrobial mode of action ppt
... bilayer plane, so the bending potential may be the driving force for penetration of the N-terminus or C-terminus of the peptides into the core of the bilayer In other words, partial conformational flexibility ... for import of the peptides into membranes or the cytosol For example, a single Pro residue has been found to be a key structural factor for the penetration of cells by buforin II [20] Also, the ... that for M17P (data not shown) Examination of the shape of the sensorgrams for M17 and M17P reveals significantly different binding kinetics In particular, the sensorgrams indicate that the initial...
- 15
- 376
- 0
Báo cáo Y học: Isolation, enzymatic properties, and mode of action of an exo-1,3-b-glucanase from Trichoderma viride doc
... intrinsic rate constant Kint Accordingly, the substrate binding affinity becomes the sum of the affinities for glucose residues of the substrate accessible for binding; the distinction of Vmax (kcat) values ... center of the enzyme According to this theory the kinetic parameters can be expressed in a unified way in terms of subsite affinities Ai of m subsites and the intrinsic rate constant Kint for glucosyl ... observed for the inverting exo-1,3-b-glucanase from T viride AZ36 Mode of action in the hydrolysis of laminarins The mode of action of the exo-1,3-b-glucanase was studied using laminarin from...
- 9
- 554
- 0
Car Ownership and Mode of Transport to Work in Ireland* potx
... geo-code of place of work, distance travelled, time of departure and mode of transport for the journey to work Mode of transport refers to the usual mode of transport for the outward journey to work ... to incorporate the endogeneity of the car ownership decision by estimating a joint model of car ownership and mode of transport to work The 2006 Census of Population also contains detailed information ... in the labour force and inward migration In terms of the implications for transport, the most striking is the increase in new vehicle registrations, which increased by over 60 per cent over the...
- 34
- 476
- 0
Báo cáo khoa học: Mode of action of the microbial metabolite GE23077, a novel potent and selective inhibitor of bacterial RNA polymerase docx
... process, for example (a) lipiarmycin inhibits the formation of the first dinucleotide of the nascent RNA chain [11], (b) rifampicin blocks the synthesis of RNA molecules longer than two or three ... inhibitor, worthy of further investigation for the wealth of structural information that it can provide on the functioning of a crucial enzyme like RNAP It would be interesting to establish whether the ... that would ordinarily follow the formation of the first phosphodiester bond, or whether it acts at a different step, as might be suggested by the lack of crossresistance Also, further information...
- 9
- 339
- 0
Báo cáo Y học: Exploring the primary electron acceptor (QA)-site of the bacterial reaction center from Rhodobacter sphaeroides Binding mode of vitamin K derivatives pptx
... resulting from the response of the protein to the QA-reduction Hence, these absorption bands are indicative for the interaction between the quinone and the protein binding pocket Therefore, structural ... nitrogen of the HisM219 side chain as found for UQ-10 in the X-ray structure The position and the orientation of the quinone rings are similar to those of UQ-10 (Fig 3) The aromatic rings of the naphthoquinones ... similarity in terms of the position and orientation x and y denote rotational axis used for the description of the quinone orientation within the QA binding site See Fig for coloring the C4-carbonyl...
- 13
- 534
- 0
stenersen - chemical pesticides - mode of action and toxicology (crc, 2004)
... produced in animals Glyphosate and other inhibitors of amino acid synthesis are therefore much less toxic in animals than in plants, and the opposite is true for the organophosphorus and carbamate insecticides ... tools for the exploration of the normal processes The intention of this book is therefore to try to collate some of the knowledge in the respective biological sciences and to explain the points ... illustrates the inborn uncertainty of such determinations All the points are well inside the line for additivity, and some kind of potentiation is evident 2.3.4.3 Atrazine and organophosphate insecticides...
- 296
- 396
- 0
Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25