... sequences of VsEndA (Fig 1) The theoretical pI was 9.61 for VsEndA and 8.62 for VcEndA Expression and purification From L Escherichia coli culture, a total of 24 and 50 mg pure recombinant VsEndA and ... characterization of the enzymes, we carried out an analysis of the NaCl requirements and pH optimum of VsEndA and VcEndA The optimum NaCl concentrations for activity were found to be 425 mm for VsEndA and ... the salt optima of the enzymes are fine-tuned to match the salinity of their environment The outer membrane and cell wall of Gram-negative bacteria not restrict passage of ions, and the periplasmic...
... cavitation than those of F excelsior (figure 3A and D), the major differences occurring for low ψ values (ψcav at –1.0 and –1.5 MPa and ψ100 at –1.8 and –4.2 MPa for A pseudoplatanus and F excelsior ... and the beginning of the afternoon, allowing a high transpiration rate (4.8 and 3.9 mmol m–2 s–1 for maple and ash trees, respectively, in June, figure 1a) In ash trees, the water potential of ... hours of the morning resulting in low transpiration rates (1.8 and 1.1 mmol m–2 s–1 for ash and maple trees, respectively, in June, figure 1c) In comparison of F excelsior, the limitation of stomatal...
... equivalent volumes of M urea solution and then run on SDS ⁄ PAGE Lanes a and d, in the absence of both TnC and Ca2+; lanes b and e, in the presence of TnC and the absence of Ca2+; lanes c and f, in the ... presence (lanes m–q) of equimolar amounts of Akazara scallop TnC Lanes h and m, ATnI-52K; lanes i and n, ATnI-19K; lanes j and o, ATnI1)128; lanes k and p, ATnI130)252; lanes l and q, ATnI232)292; ... crystallographic studiesof vertebrate troponins [19,20], which demonstrated that the Ca2+-saturated N- and C-domains of TnC bind to the regulatory and structural TnC-binding sites, respectively, of TnI, and...
... Rao and Dr J Josse for critical review of the manuscript, and Professor W Loffelhardt (University of Vienna, Austria) for ¨ his assistance in N-terminal protein sequencing and some MALDI-TOF MS ... molecular mass of 19 216 Da and a pI of 4.69, in good agreement with MALDI-TOF MS (Fig 1A) and chromatofocusing data, respectively The other three N-terminal sequences determined in this work, of Anabaena ... UÆmg)1 protein and recovery levels of 20– 30% In all cases, the analysis by SDS ⁄ PAGE and native PAGE of purified preparations showed only one protein band of 20–22 kDa (Figs and 2; supplementary...
... amplicons of 270 and 320 bp for and 10 isolates, respectively On the other hand, different sized amplicons of 100, 150, 200, 230, 240, 250, 270, 290 and 340 bp were observed for 8, 1, 1, 1, 2, 1, 1, and ... (bp) Table Pheno- and genotypic characteristics of S aureus isolates from Central Java, Indonesia and Hesse, Germany Comparativestudies on pheno- and genotypic properties of Staphylococcus aureus ... 37oC, 25 µl of proteinase K (14,8 mg/ml; Sigma, USA) and 200 µl of buffer AL (containing reagents AL1 and AL2) was added The suspension was incubated at 56oC for h and at 95oC for 10 min, and after...
... tonsils of three animals at PIDs 3, andand from the ileocoecal lymph node of one pig at PID and mesenteric lymph node of one animal at PID http://www.actavetscand.com/content/50/1/34 The results of ... pathogenicity of the virulent Weybridge andof the low virulent New South Wales strains Japanese researchers performed comparative immunohistochemi- http://www.actavetscand.com/content/50/1/34 cal studies ... of CSFV in the natural host, to obtain data ofcomparative pathology and to compare the applicability of virus detection methods These observations will contribute to a better understanding of...
... COMPARATIVE STUDIES, FORMAL SEMANTICS AND PVS ENCODING OF CSP# SHI LING (B.Eng., East China Normal University (China) , 2009) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY SCHOOL OF ... complete proof tree where all of the leaves are recognised true Each node in a proof tree is a proof goal which is a sequent consisting of a list of formulas called antecedents and a list of formulas ... and CSPM models Secondly, we characterise various reasoning techniques and verifiable properties of FDR, ProB and PAT, respectively Next, we explore the strengths and limits of the languages and...
... moniliforme and F. subglutinans in 1983. In AustraliaandintheUnitedStates,F.graminearumwascapableofcausingheadblightof wheat,crownrotofwheatand stalkrotofmaize.F.culmorumfromfootrotofwheat and barley ... old stems andoncobsofmaize.Thestrawanddebrisofwheat,barleyandothercerealarethe mainreservoirofF.graminearum.Chlamydosporesorperitheciaofthisfungusformed on debris can survive over winter and infect ... W,dropping750àlinoculumintothewhorl;LW,coatinginoculumontheleaf4thanddropping 750àlinoculumintothewhorl. 26 FactorsaffectinginfectionofFusarium 2.3.5.Effectofsiteofinoculationoninfectionandsymptommanifestationofdifferent species Results of the comparative...
... Payments andTaxEvasion In this section we examine the link between bribe payments andtaxevasion In col of Table 5, we regress taxevasion on bribes and find that the larger the percentage of sales ... Countries Abiders Tax Evasion= 0, Bribes=0 Perpetrators TaxEvasion >0, Bribes=0 Victims TaxEvasion =0, Bribes>0 Retaliators TaxEvasion >0, Bribes>0 22.98% # of Countries Bribes TaxEvasion Africa ... the true tax burden of each firm and the magnitude ofevasion As an alternative, we adjust the taxevasion measure using statutory corporate tax rates and 1-year effective corporate tax rates...
... state of CNS dis-inhibition involved in the pathophysiology of the condition [93] Genetic linkage studies involving families with a history of alcoholism show involvement of chromosomes andand ... relationship of age, gender, handedness, and sidedness to the size of the corpus callosum Academic Radiology 1994; 1:243-248 69 Driesen NR, Raz N The influence of sex, age, and handedness on ... is unclear, as most extant studies are descriptive and limited in power and generalizability Still lacking are large-scale, blinded, randomized, prospective studiesof ERP-guided therapy; however...
... deviation of patients and control group were 61.96±14.5, 59.63±13.8, respectively Age range of patients and controls were 44-85 year and 43-86 year respectively Table shows serum Na+, K+ of case and ... consisted of 155 patients suffering from age-related or senile cataract (79 female, 76 male) and the control one consisted of 155 of cases’ close relatives (79 female, 76 male) Mean age and standard ... 166 Introduction One of the often seen consequences of the aging process is the development of senile cataract, a disease that affects one of our most precious gifts, that of sight This so-called...
... a, Use of Grammar 14 a1 Modality 14 a2 Use of Active / Passive voices 14 a3 Sentence order 15 a4 Length of sentences 15 a5 Kinds of sentences 16 b Use of vocabulary 17 b1 Archaic words and phrases ... Preamble of the Convention and their realization 4.3.1 21 23 The Body 23 4.3.2.1 The Body of the Convention and its realization 23 4.3.2.2 Remarks 26 a, Use of Grammar 26 a1 Modality 26 a2 Use of Active/ ... of the discourse structure and some major linguistic features of the International Declaration on Human Rights 3.3.1 11 11 3.3.1.2 Remarks 12 The Body 13 3.3.2.1 The Body of the Declaration and...
... Justification, Relation of Cause, Purpose, Condition and Otherwise, Interpretation and Evaluation, Restatement and Summary, and lastly Sequence and Contrast The types of relation between N and S not completely ... Beginning: Title and Preamble 4.3.1.1 The Title and Preamble of the Convention and their realization * Similizing with the Title and Preamble of a Declaration, the Title and Preamble of a Convention ... type of words often appear in the works of formal style in general, in legal documents in particular Therein; hereby; thereof, whereof, notwithstanding;are some samples for archaic words and in...
... right to a standard of living adequate for the health and well-being of himself andof his family, including food, clothing, housing and medical care and necessary social services, and the right ... the Charter of the United Nations, recognition of the inherent dignity andof the equal and inalienable rights of all members of the human family is the foundation of freedom, justice and peace ... law solely for the purpose of securing due recognition and respect for the rights and freedoms of others andof meeting the just requirements of morality, public order and the general welfare in...
... on the rules and principles that regulate the practice of the critic, the art of judging with knowledge and propriety of the beauties and faults of a literature performance, or of a production ... function of communication and indicates the limits of present knowledge in society 2.2.2 Types of criticism 2.2.2.1 Practice of analyzing and evaluating literary or other artistic works This type of ... Kroeber and Kluckhohn compiled a list of over 200 different definitions of culture in their book “Culture: A critical review of Concepts and Definitions” One of these is: “Culture consists of pattern,...
... reflects discussion and results of case -studies on national institutional capacities for floods risk reduction in Japan, Russia, Thailand and Vietnam and practices of institutions and tools applied ... Evaluation of rules defining collective and individual behavior of actors and their interactions is a part of this exercise Human security of local communities and social rehabilitation of affected ... songs and dances Institutions and governance arrangements often centered on the collection of crop taxes and forced labor by nobility Institutions and cultural practices around the “management” of...