... Kawamoto A: Endothelialprogenitorcells for postnatal vasculogenesis Am J Physiol Cell Physiol 2004, 287: C572-C579 10 Aicher A, Zeiher AM, Dimmeler S: Mobilizing endothelialprogenitorcells Hypertension ... migration of EPC from RA patients in response to VEGF Interestingly, EPC from RA patients exhibited only modest adhesion to endothelialcells stimulated with TNF-α, compared with EPC from healthy ... progenitorendothelialcells for angiogenesis Science 1997, 275:964-967 Murayama T, Tepper OM, Silver M, Ma H, Losordo DW, Isner JM, Asahara T, Kalka C: Determination of bone marrow-derived endothelial...
... healthy longevity may be achieved EndothelialProgenitor Cells: Rejuvenators of the Vasculature During development endothelialcells are believed to originate from a precursor cell, the hemangioblast, ... W, Lanzer G, Guelly C, Strunk D: Blood monocytes mimic endothelialprogenitorcells Stem Cells 2006, 24:357-367 Foteinos G, Hu Y, Xiao Q, Metzler B, Xu Q: Rapid endothelial turnover in atherosclerosis-prone ... general there is a need for continual endothelial cell replacement from EPC Because the endothelialcells are exposed to enormous continual sheer stress of blood flow, mechanisms of repair and...
... circulating endothelialcells and endothelialprogenitorcells in chronic kidney disease Blood Purif 2006, 24(2):196-202 58 Verma S, Kuliszewski MA, Li SH, et al: C-reactive protein attenuates endothelial ... function of endothelialprogenitorcells in patients with migraine Neurology 2008, 70(17):1510-7 56 Esposito K, Ciotola M, Maiorino MI, et al: Circulating CD34+ KDR+ endothelialprogenitorcells correlate ... Additional file 1: Progenitor Cell Numbers Expressed as a Percentage of Peripheral Blood Mononuclear Cells The data provided represent number of progenitorcells (CD133, CD34, and cells with EPC...
... by red bloodcells lysis buffer, the cells were resuspended in PBS + 0.5% BSA + mM EDTA at a concentration of × 108 cells/ mL, and stained by EpCAM-Fitc (BD Biosciences) for 15 at 4°C Cells were ... EpCAMCK+CD45- cells were analyzed in 15 peripheral blood samples from healthy volunteers No EpCAM and CK double-positive cells could be detected in any of the samples We did not observe EpCAM+CK- cells ... EpCAM-CK+ cells in samples (2/15 = 13%) when cells were enriched by CD45 depletion The median number of CK+ cells was 2/ 10 mL blood with an overall false positive rate
... by red bloodcells lysis buffer, the cells were resuspended in PBS + 0.5% BSA + mM EDTA at a concentration of × 108 cells/ mL, and stained by EpCAM-Fitc (BD Biosciences) for 15 at 4°C Cells were ... EpCAMCK+CD45- cells were analyzed in 15 peripheral blood samples from healthy volunteers No EpCAM and CK double-positive cells could be detected in any of the samples We did not observe EpCAM+CK- cells ... EpCAM-CK+ cells in samples (2/15 = 13%) when cells were enriched by CD45 depletion The median number of CK+ cells was 2/ 10 mL blood with an overall false positive rate
... cytometry analysis of cells Either isolated cellsfrom OC were directly used for flow cytometric analysis or cells were used after adherence and cultivation, as described above Cells were washed ... -2 as negative markers to eliminate haematopoietic and endothelialcells (Table 2) The expression of progenitor typical markers varied from nearly no detectable staining to relatively high levels ... expanded progenitorcells that express antigens recognized by CD9/ CD44/CD54/CD90/CD166 monoclonal antibodies, the number of immunoreactive cells was quantified by flow cytometry Data from adherent,...
... of endothelialprogenitorcells Arterioscler Thromb Vasc Biol 2008, 28(9):1584-1595 Fadini GP, Baesso I, Albiero M, Sartore S, Agostini C, Avogaro A: Technical notes on endothelialprogenitor cells: ... endothelialprogenitor cells: ways to escape from the knowledge plateau Atherosclerosis 2008, 197:496-503 Kawamoto A, Losordo DW: Endothelialprogenitorcells for cardiovascular regeneration Trends ... fluorescence from QD cluster inside the EPC (616 nm with respect to 625 nm outside EPCs) Note that the cells were washed after QD incubation so that QDs are not expected to remain outside cells in...
... Figure Number of endothelialprogenitorcells during progression of multiple organ dysfunction syndromes The number of endothelialprogenitorcells in syndromes (left) peripheral blood and (right) ... capacity of endothelialprogenitorcells during progression of multiple organ dysfunction syndromes The angiogenic capacity of syndromes endothelialprogenitorcells in (left) peripheral blood and ... the Figure Angiogenesis function of endothelialprogenitorcells Angiogenesis function of endothelialprogenitorcells with Calcein added, observed by (a) cells phase-contrast microscope and...
... identification, enumeration, purification, and expansion of CD133+ and VEGF-R2+ endothelialprogenitorcellsfrom peripheral blood J Immunoassay Immunochem 2007, 28:13-23 Asahara T, Masuda H, Takahashi ... phycoerythrin; RBC: red blood cell; SBP: systolic blood pressure; tPA: tissue plasminogen activator; WBC: white blood cell Acknowledgements This study is supported by a program grant from the National ... with EPO treatment; group = without EPO treatment EPC = endothelialprogenitor cell; EPO = erythropoietin; RBC = red blood cell; WBC = white blood cell Yip et al Critical Care 2011, 15:R40 http://ccforum.com/content/15/1/R40...
... systemic administration of both mature endothelialcells and endothelialprogenitorcells [16,19] EPCs have also been documented to be involved in glomerular endothelial repair: bone marrow transplantation ... the endothelialprogenitor cell system in patients suffering from sepsis with acute impairment of renal function Materials and methods Patients and blood samples Blood samples were obtained from ... represent the endothelial lineage For that reason, these cells were not quantified in our current study The percentages of total circulating endothelialprogenitorcells (CD133+/Flk-1+ cells in the...
... Cells 50 2.8.2.5 Endothelial Cell Types 51 2.8.2.6 EndothelialProgenitorCells 52 2.8.2.7 EndothelialProgenitorCells in Fracture Healing 53 2.8.2.8 Proposed Endothelial Cell ... Osteogenesis-Enhancing Potential of Human Umbilical CordBloodEndothelial Colony-Forming Cells Stem Cells 2012 Sep;30(9):1911-24 - Featured Top Story in CordBlood News, Connexon, July 2012 Yuchun Liu, ... where the endothelialcells in blood vessels are constantly exposed to blood flow tangential to the endothelial monolayer This is essential in controlling many aspects during the fate of an endothelial...
... mesenchymal stem cells isolated from human umbilical cordblood Stem Cells 2004, 22(4):617-624 Nunes VA, Cavaçana N, Canovas M, Strauss BE, Zatz M: Stem cellsfrom umbilical cordblood differentiate ... CD34+ cells derived frombloodCells isolated from human umbilical cordblood were immunomagnetically selected and characterized by flow cytometry A representative subpopulation of the cells ... methods Isolation and characterization of human CD34+ cellsfrom the umbilical cordblood CD34+ stem cellsfrom human umbilical cord were obtained from healthy babies, born in Hospital Albert Einstein,...
... = 1) is shown from Suppression Assays of CD33+ or CD11b+ cells with autologous T cells, respectively * indicates statistically significant suppression of T cells by CD11b+ cellsfrom co-culture ... bloodcells are analyzed by routine flow cytometry for the presence of myeloid-derived suppressor cells (MDSC) as a marker for tumor presence Active MDSC are distinguished from normal bloodcells ... proliferation control (T cells alone), an induction negative control (CD33+ cellsfrom medium only cultures), and an induction positive control (CD33+ cells isolated from PBMC cultured with GM-CSF...
... profiles in osteoblast progenitors from umbilical cordblood stem cells by BIO-PLEX technology Cell Biol Int 2008, 32, 320-325 27 Rogers I, Casper RF Umbilical cordblood stem cells Best 393 Pract ... stem cellsfrom human bone marrow and umbilical cordblood Stem Cells 2006, 24, 679-685 Chen L, Tredget EE, Wu PYG, Wu Y Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial ... umbilical cordblood was collected during Caesarean section of pregnant female dogs Canine UCB-MSCs were produced by culturing to facilitate proliferation of mononucleated cellsfromcordblood as...
... analysis A guarantee of enough numbers of multipotent stem cells out of a very small quantity of cordblood sample from the canine umbilical cordblood is attractive In conclusion, this study provides ... characterization of canine umbilical cord blood- derived mesenchymal stem cells 22 23 24 25 26 27 28 29 30 Ryu HM Mesenchymal progenitorcells in the human umbilical cord Ann Hematol 2004, 83, 733-738 ... Isolation of multipotent mesenchymal stem cellsfrom umbilical cordbloodBlood 2004, 103, 1669-1675 Mauney JR, Volloch V, Kaplan DL Role of adult mesenchymal stem cells in bone tissue engineering applications:...