donor and collection related variables affecting product quality in ex utero cord blood banking
Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... Bio-Plex protein array system Expression of cytokines (that is, interleukin-2 [IL-2], IL5, IL-6, IL-10, IL-17, and IFN-g) was determined by the Bio-Plex 200 system, Bio-Plex mouse Cytokine 8-plex ... following a 5-minute incubation at 37°C with 0.05% trypsin/ethylenediaminetetraacetic acid (EDTA) (Gibco) and replated MSCs of C57BL/6 origin were kindly provided by Darwin J Prockop and Catherine ... injected intraperitoneally into each mouse After hours, mice were sacrificed and lymph nodes (axillary, inguinal, and mesenteric) and spleens were harvested Single-cell suspensions were obtained...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... staining (b) H1 von Kossa staining (c) hFOB H & E staining (d) hFOB von Kossa staining Figure At 4-week time point: H&E staining and von Kossa staining of sections showing scaffolds devoid of cells ... time point: H&E staining and von Kossa staining of sections showing scaffolds devoid of cells 43 Figure At week time-point: (a) H&E staining showing newly formed tissue within the ... scaffold (arrow showing scaffold remains) (b) von Kossa staining and counterstaining with nuclear fast red showing deposition of mineralized tissue in the area marked and shown in higher magnification:...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... reported in human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs ... containing genes upregulated in EB (node C) included the genes HAND1, HOXA1, HOXB2, MSX1, MSX2, MEIS1, FGF9 and FREM1 which are involved in morphogenesis and development [36-39] This node also included ... highly expressed in adult cells, although it was reported to be enriched in hES cells [17] Actually, miR-222 was also expressed in multiple adult cell lines [16], forebrain and midbrain [45], and...
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báo cáo khoa học: " MicroRNAs involved in neoplastic transformation of liver cancer stem cells" pot
... paraformaldehyde, routinely processed and stained with hematoxylin and eosin (H&E) for histological examination by two pathologists, blinded to the results of the study, in order to verify the ... CK-7 protein expression in SP cells and non-SP cells The relative expressions of protein were calculated through comparing with GAPDH protein Li et al Journal of Experimental & Clinical Cancer ... demonstrating the existence of SP cells in both fetal liver cells and in primary rodent HCC cancer cells induced by chemical carcinogens Since the HCC cancer cells and fetal liver cells used in our...
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Báo cáo y học: " In-vivo kinetics of inhaled 5-Aminolevulinic acid-Induced Protoporphyrin IX fluorescence in bronchial tissue" pdf
... 500 time [min] Values of PPIX-fluorescence in normal findings, findings ≤ mild dysplasia and findings ≥ moderate dysplasia plotted against the Figure time between 5-ALA application and spectroscopy ... values with discriminating differences between normal and pathologic findings As reported in other studies [6,9,17], there is always an increase in sensitivity and a decrease in specificity when, ... spectroscopy Values of PPIX-fluorescence in normal findings, findings ≤ mild dysplasia and findings ≥ moderate dysplasia plotted against the time between 5-ALA application and spectroscopy Fitted curves...
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Báo cáo khoa học: "Comparative study on the in vitro and in vivo properties of two bovine herpesvirus-5 reference strains" pptx
... histological examination, the samples were fixed in 10% neutral buffered formalin, prepared by routine methods for histology, paraffin embedded, sectioned at μm and stained with hematoxylin and eosin The ... caused by infectious bovine rhinotracheitis virus in calves in Hungary Acta Vet Acad Sci Hung 1969, 19:145-151 Moretti B, Orfei Z, Mondino G, Persechino A: Infectious bovine rhinotracheitis, clinical ... sulfolipocyclodextrin in a squalane -in- water and water -in- mineral oil emulsions for BHV-1 vaccines in cattle Vaccine 2000, 19:132-41 32 Reed CJ, Muench HA: A simple method of estimating fifty per...
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Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier
... were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo pharmacokinetic study ... novel hydrophobized polysaccharide/oncoprotein complex vaccine induces in vitro and in vivo cellular and humoral immune responses against HER2-expressing murine sarcomas Cancer Res 58:3385–90 Gustafson, ... stability and toxicity of PANs, and further investigated the sustained release behavior in vivo of drug loading in PANs PA was firstly synthesized and characterized by FT-IR and 1H NMR, and then...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... acrylamide in the separating and stacking gels, respectively In order to estimate the ratio between the amounts of UP12 and the total amount of protein in the extracts, a series of samples containing ... possibly due to nutrient exhaustion In order to examine whether UP12 is induced by starvation, we tested the expression of UP12 in cells grown in minimal media containing limited concentrations ... expression is induced in response to toxic agents and increased temperature Next, we examined the effect of toxic agents on UP12 expression As shown in Fig 5, the addition of DNP or CCCP to exponential...
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Báo cáo khoa học: In vivo production of catalase containing haem analogues pot
... protein, in binding porphyrins It is not known how haem is transported intracellularly in bacteria, and the mechanisms by which haem is incorporated into soluble and membrane-bound proteins inside ... compounds being taken up into the cell and incorporated into vital haem proteins instead of haem [5] We show here that haem analogues are indeed taken up and can be incorporated into haem proteins, ... catalase proteins containing Fe-meso and Ru-meso Supplementation of the haem-free growth medium with PP resulted, much to our surprise, in PP-containing catalase, i.e protein containing a metal-free...
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Báo cáo khoa học: A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae ppt
... one binding site (A) and genes with two binding sites (B) Solid circles and solid triangles denote experimental data for a-galactosidase expression level for genes with one binding site and for ... concentration of Gal3p in the wild-type thus reducing the protein expression as in Model III This confirms the evidence obtained through gel filtering and cross-linking that Gal3p is monomeric in solution ... results with experimental data for fractional protein expression of wild-type for genes with one binding site (A) and genes with two binding sites (B) Solid circles and solid triangles denote experimental...
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Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx
... selenocysteine residue in a C-terminal Sel-tag [18] Der p carrying the Sel-tag had an intact core sequence and maintained allergen-specific IgE-binding epitopes and the use of a Sel-tag enabled labelling ... lymph node proteins revealed a radioactive band of 25 kDa in liver and bands of 30 and 56 kDa in thoracic lymph nodes (Fig 5C) No radioactive protein bands could be detected in spleen samples ... deposited in the airways of mice and metabolized The labelling method involves the incorporation of a selenocysteine residue and the gamma-emitter selenium-75 (75Se) within an engineered C-terminal...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... 30 and 60 digestion with trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D (extrinsic ... leave room for altered protein–protein interactions, which may explain why the Chlamydomonas loop region is exposed to trypsin in the in vitro assay The in vitro import experiments suggested that ... 386 and 387, or at the extreme C-terminus of PSI-G (Fig 1) These cassettes were both cloned into binary vectors under the control of the 35S promoter and terminator, and into vectors for in vitro...
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Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis pot
... [27] In rats Fig Western blot analysis of p53 and c-Jun in liver and lung Total protein extracts were obtained as described in experimental procedures A single band of about 53 kDa was detected in ... could explain in part the decreased expression of c-Jun found in chronic19 vitamin A-deficient rats The molecular mechanism that explains the decrease in C-H-Ras expression found in chronic-vitamin ... liver and lung indicating that the amount of p53 protein was significantly increased in the liver and lung from vitamin A-deficient rats that in controls c-Jun protein was significantly decreased in...
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Báo cáo Y học: In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis docx
... membrane properties As in past reports, the joint use of mutants and inhibitors acting on the same biosynthetic points has proved to be a useful way of distinguishing the in uence of direct effects, ... complex lipids (Fig 1) A close correlation (r ¼ 0.98) was observed, and this was independent of the kind of genetic lesion or inhibitor used, suggesting that this activating effect was indeed ... using thyroglobulin as the standard Except where indicated, all experiments were performed at least in triplicate RESULTS It was previously observed that changes in sterol composition increased plasma...
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Báo cáo khoa học: A study of microRNAs in silico and in vivo: bioimaging of microRNA biogenesis and regulation doc
... vector containing three tandem repeat regions of miRNA23 binding sequence (reprinted with permission [11]) information for understanding the miRNA biogenesis related to human diseases Imaging miRNA ... Several reporter-based imaging probes, including luciferase for optical imaging and sodium iodide symporter and herpes simplex virus 1-thymidine kinase for radionuclide imaging, have been widely used ... miRNA during neuronal development in living subjects In this context, noninvasive bioimaging systems using neuron-specific miRNAs could be applied not only to monitor the functional roles and production...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
... previously to induce an increase in the level of [Ca2+]i and a correlated calpain activation [28] This increase in [Ca2+]i is more intense in aorta than in brain Under these conditions, in brain, no ... promoting NO production also induce calpain activation Thus, the formation of NOS–HSP90 complexes may provide a new insight into the understanding of the mechanisms involved in modulating NO production ... immunoblotting Desmin and talin were detected with specific mAbs involved in such complexes by submitting samples of crude extracts of rat brain and aorta to gel filtration chromatography As shown in Fig...
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báo cáo hóa học:" In vivo properties of the proangiogenic peptide QK" pdf
... both in vitro and in vivo The two main VEGF receptors are VEGFR-1 or fms-like tyrosine kinase (Flt-1) and VEGFR-2 or fetal liver kinase (Flk-1) also known as kinase-insert domain-containing receptor ... Medicine 2009, 7:41 new capillaries) and arteriogenesis (enlargement and remodeling of pre-existing collaterals) [2] In detail, the term angiogenesis refers to the sprouting, enlargement, or intussusceptions ... physical training or β2 adrenergic receptor overexpression in ischemic hindlimb (HL), have shown to improve collateral flow [3,5-7] In spite of all, clinical trials using gene or protein therapy...
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Báo cáo hóa học: " In vivo evidence of htid suppressive activity on ErbB-2 in breast cancers over expressing the receptor" potx
... cytoplasmic expression Dif- Page of 13 ferently, using the anti-ErbB-2 antiserum cross reacting with the murine receptor resulted in a faint stain of the cytoplasm and rarely in staining of the ... therefore players in the signalling they induce In this context a preliminary comparative expression analysis of hTid, EGFR and ErbB-3 we have conducted in triple negative breast tumors and in cutaneous ... showed positive staining for antiTid in 40% of the cases investigated (Figure 1B-D) Correlation between htid and ErbB-2 expression in breast and non-breast tumors over expressing the ErbB-2 receptor...
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báo cáo hóa học:" Function of anterior talofibular and calcaneofibular ligaments during in-vivo motion of the ankle joint complex" docx
... the flexion-extension arc of motion They demonstrated increased strain in the ATFL with increasing plantarflexion, inversion and internal rotation Strain in the CFL was found to increase in dorsiflexion ... more in plantarflexion and supination (combination of plantarflexion, inversion and internal rotation) whereas the CFL elongates more in dorsiflexion and pronation (combination of dorsiflexion, ... foot turning upward), maximal supination (foot position that combines inversion, plantar flexion and internal rotation) and maximal pronation (combination of eversion, dorsal flexion and external...
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báo cáo hóa học:" In vivo evaluation of a vibration analysis technique for the per-operative monitoring of the fixation of hip prostheses" pdf
... system for intra operative manufacturing and stability testing of hip prostheses In Proceedings of TEHNOMUS XIII, the 13th International conference on New technologies and products in machine manufacturing ... insertion stages and canal and reinserting the prosthesis The FRF had a normal evolution during the reinsertion and the graphs corresponding to the final two stages, labelled as stage 4a and stage 5a, ... rely mainly on their clinical experience, which consists mainly of a sense of mechanical stability when exerting axial force and/ or torque on the prosthesis Moreover, excessive press-fitting of...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose