dna the variability of the dna sequence and the independent inheritance of gene alleles by siblings
Global orchestration of gene expression by the biological clock of cyanobacteria Carl Hirschie Johnson pot
... condensation and coiling of DNA [19] It is well known that changes in the local supercoiling status of DNA can affect the transcriptional rate of genes [20], and our findings concerning the behavior of ... [15] and the lab of Susan Golden [21], in addition to the study of Nakahira and coworkers [16], are consistent with this hypothesis, namely that the condensation or supercoiling status of the ... modulation of the transcription rates of all genes, accounting for the global regulation of gene expression [22] Gene- specific cis-regulatory elements that mediate rhythmic gene expression might therefore...
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Redox regulation of estrogen receptor alpha and sodium hydrogen exchanger 1 gene expression by hydrogen peroxide
... 1.1.2 The antioxidant system The amount of reactive species in the cell is determined by the rate of their production and the rate of clearance by various antioxidant enzymes and compounds in the ... distribution of glutathione peroxidases and peroxiredoxins throughout the cell 1.2 REDOX REGULATION OF GENE EXPRESSION The regulation of gene expression is the control of the level and timing of appearance ... hydrophobic ligand-binding pocket The AF-2 in the LBD of ER is composed of amino acids in helix 3, 4, and 12 The position of helix 12 changes when ER binds to ligands and therefore is the key in...
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Báo cáo hóa học: " Research Article Analysis of Gene Coexpression by B-Spline Based CoD Estimation" pot
... between the curves in the case of CoD-B and in the random case genes identified by CoD-Q, the curves of cumulated probability laid between the curves in the case of CoD-B and the curve in the random ... one uses the MSE metric, then CoD is the ratio of the explained variation to the total variation and denotes the strength of association between predictor genes and the target gene Mathematically, ... F(x) of the expression level y of gene g y given the expression level x of gene gx in the (x, y) plane We then computed CoD to determine how well the expression of gene g y is predicted by the...
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Báo cáo hóa học: " Research Article Analysis of Gene Coexpression by B-Spline Based CoD Estimation" ppt
... between the curves in the case of CoD-B and in the random case genes identified by CoD-Q, the curves of cumulated probability laid between the curves in the case of CoD-B and the curve in the random ... one uses the MSE metric, then CoD is the ratio of the explained variation to the total variation and denotes the strength of association between predictor genes and the target gene Mathematically, ... F(x) of the expression level y of gene g y given the expression level x of gene gx in the (x, y) plane We then computed CoD to determine how well the expression of gene g y is predicted by the...
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Regulation of gene expression by esrrb in embryonic stem cells
... these cells, the modulators regulated by Nanog can be identified and the interconnection of Nanog with the LIF and BMP signals can be dissected One of the potential applications of the gain -of- function ... developed and extensively applied in the study of ES cell biology 1.3.1 Alteration of gene expression by genetic perturbation There are two main strategies of genetic perturbation used for the study of ... biology They are the ‘gain -of- function’ approach and the ‘loss -of- function’ approach Most gain -of- function studies involve introducing the cDNA of a gene into cells, ideally resulting in the hyper-activation...
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báo cáo khoa học: " DNA sequence diversity and the origin of cultivated safflower (Carthamus tinctorius L.; Asteraceae)" ppsx
... subtraction by maximum likelihood; the remainder of the alleles were determined by cloning Despite the occurrence of some incongruities between loci, the NJ, ML and Bayesian trees based on the combined ... detected by the presence of double peaks and coded following the conventions of the International Union of Biochemistry and Molecular Biology From these, haplotypes were resolved using the maximum ... identities of their progenitors are of great value in both basic and applied research programs For example, the comparative analysis of crop plants and their wild progenitors can shed light on the genetic...
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Báo cáo y học: "All motifs are not created equal: structural properties of transcription factor - dna interactions and the inference of sequence specificity" doc
... bound to DNA had been determined by x-ray crystallography, and computed the number of contacts between each base in the bound DNA and the protein For each factor they assembled collections of sequences ... to be bound by the protein, computed PWMs from these sequences, and showed that there is a strong correlation between the number of contacts at a position in the bound sequence and the information ... identified by a K by K transition matrix ( K is the number of components in the Dirichlet mixture) and marginal distribution of the class variable at the first position The number of prior classes (the...
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Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot
... Tropel and J R van der Meer A B Fig (A) Genetic organization of the hbp genes in Pseudomonas azelaica strain HBP1 Open and grey bars (some as arrows) depict the orientation and the size of the genes; ... imperfect palindromes of 16 bp and a spacing of between 29 and 42 bp between their centres (Fig 1A) [20] The regions covered by XylR on the Pu and of HbpR on the PhbpC promoter in DNaseI footprinting ... larger than the sequences of the palindromes [19,21], but the exact contribution of nucleotides in the binding site contacted by the proteins is not known The current hypothesis of hexameric...
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Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot
... (a) DNA polymerase activity, which can be both RNA and DNA dependent; and (b) RNase H activity that selectively hydrolyzes the RNA strand of the RNA :DNA hybrid formed during synthesis of the ... in the presence of different concentrations of efavirenz and in the absence () or presence of 1.9 lM (s), 3.7 lM (.) and 7.5 lM (4) K-49 (C) YonetaniTheorell plot of the interaction of KNA-53 and ... different, and the contribution of the pp stacking interaction is lost Furthermore, the low number of good contacts with RT leads to instability of the complex and the compound can be easily washed off...
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Báo cáo khoa học: DNA binding and partial nucleoid localization of the chloroplast stromal enzyme ferredoxin:sulfite reductase pptx
... synthetic dsDNA as a probe (Fig 5A,B) Various amounts of recombinant PsSiR and 40-mer or 20-mer dsDNA were mixed, and then the mixtures were electrophoresed The intensity of the shifted bands ... studies of the DNA binding of sulfite reductase [1,27] Here, we compared the DNA- binding activity of ZmSiR and PsSiR (Fig 5, right) An increase in the intensity of the shifted bands was found as the ... corresponding to the first 18 residues of the N terminus and the last 16 residues of the C terminus of the predicted mature protein No peptide corresponding to the predicted transit peptide sequence was...
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Báo cáo khoa học: Constitutive expression of the human peroxiredoxin V gene contributes to protection of the genome from oxidative DNA lesions and to suppression of transcription of noncoding DNA pdf
... decreases formation of 8-oxoG in mitochondrial DNA [20], but the role of this protein in protecting nuclear DNA from oxidation in human cells and the mechanisms of regulation of the PRDX5 gene are not ... down-regulation of this gene clone, and 2608 analyzed the concentration of 8-oxoG and transcription of some families of DNA repeats in these clones Results PRDX5 expression is not induced by oxidative ... expression of the PRDX5 gene contributes to the antioxidant defense of the human genome The 8-oxoG content of nuclear DNA from mouse tissues estimated by quantitative analysis (HPLC and electrospray...
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Báo cáo sinh học: " Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" pdf
... and the effect on RNA amplification by RT-PCR (Table 4) An incubation of 10 eliminated DNA detection at the lowest concentration and increased the CT by a mean of 6.58 cycles for the other DNA ... RNA Effect of incubation temperature and time on DNA degradation and RT-PCR amplification of RNA The effect of temperature on DNA degradation and RNA amplification in the presence of UNG was ... contained 1,000 copies of amplicon DNA The fluorescence signal generated by amplicon DNA is indicated by the open form of the same symbol for each respective reaction The horizontal line at approximately...
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báo cáo hóa học:" Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" ppt
... and the effect on RNA amplification by RT-PCR (Table 4) An incubation of 10 eliminated DNA detection at the lowest concentration and increased the CT by a mean of 6.58 cycles for the other DNA ... RNA Effect of incubation temperature and time on DNA degradation and RT-PCR amplification of RNA The effect of temperature on DNA degradation and RNA amplification in the presence of UNG was ... contained 1,000 copies of amplicon DNA The fluorescence signal generated by amplicon DNA is indicated by the open form of the same symbol for each respective reaction The horizontal line at approximately...
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Báo cáo khoa học: "Protection of chicken against very virulent IBDV provided by in ovo priming with DNA vaccine and boosting with killed vaccine and the adjuvant effects of plasmid-encoded chicken interleukin-2 and interferon-g" doc
... score, and the presence of IBDV RNA in the bursae, as well as the survival rate We found that 100% of the chickens in the in ovo DNA vaccine plus boost group and 80% of the chickens in the in ovo DNA ... appear to be affected by the types and combined methods of vaccines and adjuvants, the route and time of inoculation, the promoter of the plasmid expressing the cloned genes, and animal species ... expression of SV40 and CMV in myoblasts significantly reduced by the addition of INF-γ [7] Therefore, ChINF-γ expressed by pcDNA-ChINF-γ co-delivered with the DNA vaccine may inhibit the expression of...
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Báo cáo khoa học: "Evaluation of the nuclear DNA content and GC percent in four varieties of Fagus sylvatica L" potx
... DNA content in the tortuosa variety is the same as in the common beech Furthermore, in replicated analyses of common beech with or without tortuosa, the coefficients of variation were tight and ... histograms, the average coefficient of variation for the peak of 2C nuclei for Petunia was 2.2 % and that of Fagus 3.1 %, altogether acceptable 2C DNA values converted to pg amounts and to Mbp ... 0.01 pg and of Q petraea: 2C 1.87 ± 0.02 pg Using microdensitometry methods, Greilhuber evaluated the genome of Q petraea to 2C 1.8 pg [16], and Ohri and Ahuja [20] to 1.58 pg Although their DNA...
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Báo cáo lâm nghiệp: " A flow cytometric evaluation of the nuclear DNA content and GC percent in genomes of European oak species" pptx
... procedures for DNA polymorphism analysis or the study of genetic architecture of genomes by RFLP and PFGE (choice of rarely cleaving restriction enzymes) or RAPD (choice of primers), and in speciation ... presently not possible The GC% values presented here are the first for the genus Quercus and the Fagaera Therefore, family The values are typical for higher plants This type of data can be useful ... of Quercus plants on the basis of genome size relative to a standard plant of known ploidy MATERIALS AND METHODS The plant material was leaves of in vitro cloned plantlets of Q petraea (Matt)...
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Báo cáo khoa học: "The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis" pdf
... initial DNA released minus residual DNA released without the interference of apoptosis On the other hand, the intensity of apoptosis could be easily estimated by subtracting the total DNA released ... agarose-encapsulated cells The range of DNA quantification, beginning just before the compression zone (CZ) and ending at the bottom of the smears, is shown (B) The amount of released DNA (optical density, ... the fragility of agarose plugs, the corners tended to break off easily Therefore, to ensure that the same number of cells (DNA) per lane was loaded into a gel, we cut a section of the better preserved...
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Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps
... such as DNA of Pseudomonas poae, Delftia acidovorans, and Burkholderia cepacia A detailed sequence analysis of the PCR-positive samples of ReA and UA patients revealed a number of DNA of bacteria ... indicate the number of patient set from whom bacteria were detected aAccession number of the bacterial species in the EMBL database bLength of alignment on which the 16S rDNA inserted sequence and the ... valuable overall picture of the bacterial DNA environment present in the SF of the actively inflamed joints of arthritis patients Characterization of the DNA reveals a wide spectrum of organisms so far...
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Báo cáo y học: "DNA transposons and the role of recombination in mutation accumulation in Daphnia pulex" pdf
... higher rates of both DNA transposon gain Page of 13 and loss than their asexual counterparts We describe the general landscape of DNA transposons in D pulex, survey the relative abundance of each ... determined by structural motifs such as, in the case of CACTA elements, terminal inverted repeats (Figure 1) [21] and, in the case of Helitrons, palindromes and the identification of tandem arrays of ... outputs from BLASTN (e-value < 0.00001 and >20% of the length of the query) and RepeatMasker (RM; >50 bp in length, >70% similarity, and >20% of the length of the query), respectively Families with...
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Báo cáo y học: " Initiation of bacteriophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx
... an array of PCR fragments, they monitored the accumulation of nascent DNA across the entire viral genome over the course of infection, allowing both the origins and breadth of DNA synthesis to ... on the displaced strand of the D-loop allows lagging strand synthesis (panel A) In bubble-migration synthesis, lagging strand synthesis does not occur, and the newly synthesized single strand ... from the back of the D-loop as new DNA is synthesized at the front of the D-loop (panel B) In this and subsequent figures, new leading strand replication is in solid red and new lagging strand...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose