... Management) It is viewed as a collection of autonomous and intelligent agents located in specific network entities These agents co-operate and communicate in order to perform intrusion detection ... of agents reflect a set of properties, which common among them and are described below [12]: - Autonomy: is the ability of an agent to operate without direct intervention of humans or other agents ... which is responsible for managing interactions with the other agents and the Social Manager, which holds information about the other agents, support inter-agent communication facilities in the...
... monocytogenes 4b, and to compare the sensitivity and specificity of the sandwich ELISA for screening Listeria spp using different sets of flagella specific antibody combinations at 7,000 rpm for 30 ... room temperature (RT) After Development of a sandwich ELISA for the detection of Listeria spp usingspecific flagella antibodies 43 washing, 100 µl of each HRP conjugated MAb was added into each ... MAbs into three groups by specificity against epitopes [MAb 2B1 (I), MAb 4F12 (II) and MAbs 3B7, 6F3, 7A3 (III)] Three MAbs in third group (3B7, 6F3 and 7A3) Table Specificity analysis of antibodies...
... Ratio specific antigen in a community-based population of healthy men Establishment of age -specific reference ranges JAMA 1993; 270: 860–4 Lilja H, Christensson A, Dahlén U, et al Prostate -specific ... serum prostate -specific antigen The clinical value of percent free prostate -specific antigen Urol Clin North Am 1997;24:353–65 Vessella RL, Lange PH Issues in the assessment of prostatespecific antigen ... Luderer AA, Thiel RP, Carlson G, Cuny CL, Soriano TF Using proportions of free to total prostate -specific antigen, age, and total prostate -specific antigen to predict the probability of prostate...
... (Gly4Ser)3 VH Figure 2Structure of an scFv cassette spliced using a PanelSer)3 hinge (Gly4 A: Panel A: Structure of an scFv cassette spliced using a (Gly4Ser)3 hinge Panel B Alternative glycine codons ... g/ml 750 g/ml Concentration of scFv Figure virus with anti-gD scFv Specific reduction of HSV-2 plaque numbers by incubation of Specific reduction of HSV-2 plaque numbers by incubation of virus ... used clinically The specific nature of anti-herpes scFv and the ability to choose an inert formulation has two potential advantages over other microbicides First is selected high specific activity...
... customized AFO manufactured using SLS was presented by Faustini et al [14] The geometry of these AFOs was captured by Computed-Tomography (CT) scanning of an AFO built using a conventional technique ... already been performed in the area of RP patient -specific orthoses, several aspects of the implementation of the technique to manufacture AFOs using RP need to be addressed including: a) demonstrating ... experiments to evaluate the mechanical effect of orthoses manufactured using RP and compare their performance with that achieved using orthoses fabricated by means of conventional techniques Current...
... homeostasis in autism spectrum disorders Trends Genet 2010, 26:363-372 Betancur C: Etiological heterogeneity in autism spectrum disorders: more than 100 genetic and genomic disorders and still counting ... children with autism spectrum disorders (ASD) characterized by fluctuating behavioral symptoms and cognitive skills following immune insults Some of these children also exhibit specific polysaccharide ... their neuropsychiatric symptoms Keywords: autism spectrum disorders (ASD), cytokine, innate immunity, transcription profiling, monocytes, specific polysaccharide antibody deficiency (SPAD) Background...
... (INOS), and cyclooxygenase-2 (COX-2) using canine sequences available in Genbank If canine specific sequence was not available, then degenerate primers were developed using sequence data available for ... is the earliest time point reported for site -specific gene expression analysis in dogs using this model Previous chondrocyte gene expression analysis using the ACL-X transection model within a ... was eluted off the column using 30 μl of RNase free water The yield of extracted total RNA was determined by meas- uring absorbance at 260 nm, and purity was assessed using the ratio of absorbance...
... (Gly4Ser)3 VH Figure 2Structure of an scFv cassette spliced using a PanelSer)3 hinge (Gly4 A: Panel A: Structure of an scFv cassette spliced using a (Gly4Ser)3 hinge Panel B Alternative glycine codons ... g/ml 750 g/ml Concentration of scFv Figure virus with anti-gD scFv Specific reduction of HSV-2 plaque numbers by incubation of Specific reduction of HSV-2 plaque numbers by incubation of virus ... used clinically The specific nature of anti-herpes scFv and the ability to choose an inert formulation has two potential advantages over other microbicides First is selected high specific activity...
... Measurement of beta-amyloid peptides in specific cells using a photo thin-film transistor Chang-Beom Kim†1, Cheol-Joo Chae†1, Hye-Rim Shin1, and ... out for h to protect nonspecific antibody bindings in the next step Then, the overnight incubation with a primary antibody (Abcam, Cambridge, MA, USA) was performed for the specific binding to Aβ ... with FITC, the nuclei of the HeLa cells were stained using 4′,6-diamidino-2-phenylindole [DAPI] (Figure 2d), and the p-FET sensor was imaged using a fluorescent microscope and was irradiated by...
... antigen -specific Tol-DC in vitro using LF, and to use these cells as therapeutic tools to inhibit RA In the present study, we evaluated the ability of LF to generate a population of Tol-DC Using ... groups of mice were measured with a standard microtiter assay using either draining lymph node cells or splenocytes, using KLH or CII, and using 3H-labeled thymidine T cells at × 105/well were seeded ... for the generation of an antigen -specific tolerogenic treatment approach using LF-treated DC This is the first demonstration that in vitro-generated antigen -specific immature DC may be used as...
... > 5% in patients using IGTVMIP, patients using IGTV2Phases, and patients using IGTVMIP-Modified Volumetric underestimation > 10% occurred in patients using IGTVMIP, patient using IGTV2Phases, ... using either IGTVMIP or IGTV2Phases in 15 patients, but in no patients when IGTVMIP-Modified was used Volumetric underestimation > 20% occurred in patients using the IGTVMIP and in patients using ... the individual phase images as described elsewhere [5,6] Patient -specific IGTV determination We determined patient -specific IGTVs using the demonstrable extent of tumor motion shown in the 4-D...
... and therefore cannot realistically be used as a screening tool Using compartment -specific features of fixed flexion radiographs (specifically JSN and subchondral sclerosis), however, the SRM increased ... using much lower sample sizes if only participants with a high likelihood of fast progression are recruited The objective of the current study was therefore to determine whether compartment -specific ... (JSN, osteophytes, sclerosis, and others) at baseline, and compartment -specific structural status of the knee cartilage in MRI (specifically denuded subchondral bone area and cartilage thickness)...
... position using the first 10 million alignments for each lane The regression model was fitted using only the most highly expressed transcripts, as these have the best signal-tonoise ratio [8] Specifically, ... al Genome Biology 2011, 12:R13 http://genomebiology.com/2011/12/2/R13 specific deviations from uniform sampling of reads using the model described in [8] but can flexibly accommodate other models ... start positions (Figure 1): (a) Expression estimation using alignments to a predefined transcriptome reference, (b) Expression estimation using alignments to a transcriptome reference that is...
... using a Student’s t test Differences in survival between experimental groups were analyzed using the Kaplan-Meier approach The statistical significance of group differences will be assessed using ... and antigen -specific immunotherapy Mol Ther 18:692-699 17 Tseng JC, Hurtado A, Yee H, Levin B, Boivin C, Benet M, Blank SV, Pellicer A, Meruelo D: Using sindbis viral vectors for specific detection ... resulting in activation of tumor -specific immunity [20-22] The observed antitumor effects generated by doxorubicin-loaded tumor cells may also be contributed by tumor -specific immunity Recent studies...
... chemotherapeutic drugs including microtubule-targeted agents can induce cell death [11] These can be classified mainly into two groups [12]: microtubule-depolymerizing agents (MDAs), including vinca alkaloids ... culture reagents were obtained from GIBCO/BRL Life Technologies (Grand Island, NY) unless otherwise indicated Colchicine, DMSO, and thiazolyl blue tetrazolium bromide (MTT), and other chemical agents ... challenge Tumor cell lysis by cytotoxic T lymphocyte (CTL) Cytotoxicity assays for specific cell lysis were performed using the DELFIA EuTDA cytotoxicity method [35] B16 cells were trypsinized and...
... surface was visualized using pseudo-coloring To reduce missing wedge artifacts, so-called streaks, the surface was smoothed using a × × averaging of voxels (downsampling) Using the ‘Create Surface’ ... results in anisotropic resolution in the resulting reconstruction [7] Only for specific samples that were properly shaped using a focused ion beam and mounted in a special holder, these subsampling ... reduced using a small tilt increment and maximizing the tilt range of the electron tomogram Because gold and silica NM are hardly sensitive to radiation damage, extensive data collection using...
... prevalence of specific mental disorders Thus, there is a need to confirm these results by ascertaining the prevalence of psychiatric disorders in a large epidemiological study using detailed ... to assess the prevalence of specific common mental disorders and post traumatic stress disorder (PTSD) symptoms and associated risk factors in UK Service personnel using a two-stage epidemiological ... prevalence of psychiatric disorders between those who deployed to the main fighting period of the Iraq War and those who did not (assuming a prevalence of common mental disorders of 20%) This allowed...
... performing an occlusal adjustment, the occlusion was examined using a model attached to the articulator with a BPOP record obtained using a wax registration material (Bite Wafer; Kerr U.S.A., Romulus, ... BPOP before and after treatment were tested using McNemar’s test Differences in subjective and clinical variables before and after treatment were tested using the Wilcoxon’s signed-rank test Spearman’s ... aural symptoms after occlusal equilibration using a BPOP reference position [13] In the present study, excellent short-term results were obtained using occlusal adjustments made with a BPOP reference...
... http://respiratory-research.com/content/11/1/72 we designed a piece of equipment for specific inhalation challenges with occupational agents that can allow for all types of generation within the same instrument ... consecutive patients for whom the cause of OA was one or another of the occupational agents that could be generated using GenaSIC There was therefore no selection of patients and there was no instance ... subject using the new equipment (no significant changes in spirometry, responsiveness to metacholine and induced sputum cells), the subject was exposed in a "realistic way" [5] for two hours using...