directed evolution of adeno associated virus aav as vector for muscle gene therapy
Báo cáo sinh học: " The state of the art of adeno-associated virus-based vectors in gene therapy" ppt
... transfer vector Recombinant adeno- associated viral (rAAV) vectors have rapidly advanced to the forefront of gene therapy in the past decade The exponential progress of AAV- based vectors has been ... especially adenovirus and adeno- associated virus Since the adeno- associated virus was first isolated and its biological properties established, it has been considered a promising vector for gene therapy ... of infectious adeno- associated virus genomes in bacterial plasmids Gene 1983, 23:65-73 Hermonat PL, Muzyczka N: Use of adeno- associated virus as a mammalian DNA cloning vector: transduction of...
- 7
- 484
- 0
Báo cáo hóa học: " The state of the art of adeno-associated virus-based vectors in gene therapy" doc
... transfer vector Recombinant adeno- associated viral (rAAV) vectors have rapidly advanced to the forefront of gene therapy in the past decade The exponential progress of AAV- based vectors has been ... especially adenovirus and adeno- associated virus Since the adeno- associated virus was first isolated and its biological properties established, it has been considered a promising vector for gene therapy ... of infectious adeno- associated virus genomes in bacterial plasmids Gene 1983, 23:65-73 Hermonat PL, Muzyczka N: Use of adeno- associated virus as a mammalian DNA cloning vector: transduction of...
- 7
- 362
- 0
Báo cáo y học: "Substitution of adeno-associated virus Rep protein binding and nicking sites with human Chromosome 19 sequences" pps
... Falck-Pedersen E: Adeno- associated virus site-specific integration and AAVS1 disruption J Virol 2004, 78:7874-7882 Owens RA: Second generation adeno- associated virus type 2-based gene therapy systems ... required for synthesis of adeno- associated virus VP1 capsid protein J Virol 1988, 62:3356-3363 Mendelson E, Trempe JP, Carter BJ: Identification of the trans-acting Rep proteins of adeno- associated virus ... Kotin RM: The Rep78 gene product of adenoassociated virus (AAV) self-associates to form a hexameric complex in the presence of AAV ori sequences J Virol 1997, 71:4461-4471 Page 10 of 11 28 Weitzman...
- 11
- 362
- 0
Báo cáo khoa học: Adeno-associated virus gene transfer in Morquio A disease – effect of promoters and sulfatase-modifying factor 1 pot
... T, Kobayashi E, Hoshika A & Ozawa K (2004) Adeno- associated virus (AAV) vector- mediated liver- and muscle -directed transgene expression using various kinds of promoters and serotypes Gene Ther ... (2005) Adeno- associated virus vectors in clinical trials Hum Gene Ther 16, 541–550 Promoter and SUMF1 effect on Morquio gene transfer 34 Wu Z, Asokan A & Samulski R (2006) Adeno- associated virus ... self-complementary adenoassociated viral vectors Hum Gene Ther 20, 1697–1702 Alexander I, Cunningham S, Logan G & Cristodoulou J (2008) Potential of AAV vectors in the treatment of metabolic disease Gene Ther...
- 12
- 460
- 0
Characterization of Fabry mice treated with recombinant adeno-associated virus 2/8-mediated gene transfer pdf
... inconvenience of this treatment as a result of the requirement for continued administration of large doses of enzyme necessary for therapy Therefore, gene therapy for Fabry disease has been explored ... injected with × 1012 particles of rAAV 2/2-hAGA, × 1011 particles of rAAV 2/8-hAGA, or × 1012 particles of each rAAV 2/8-hAGA vector The genomic dosage of the viral vector was identified at 6, 12, and ... overcome before rAAV vector- mediated gene therapy can be used clinically Efficient and versatile large-scale AAV vector- production systems are needed for clinical application of this vector [36]...
- 10
- 181
- 0
Báo cáo khoa học:" Transduction of rat pancreatic islets with pseudotyped adeno-associated virus vectors" doc
... helper virus, such as an adenovirus or herpesvirus [11] AAV2 has not been associated with a human disease There are conflicting reports regarding the ability of AAV2 vectors with an AAV2 capsid (AAV2 /2) ... translocation of adeno- associated virus type J Virol 2002, 76:11505-11517 Gao GP, Alvira MR, Wang L, Calcedo R, Johnston J, Wilson JM: Novel adeno- associated viruses from rhesus monkeys as vectors for ... University of North Carolina, USA) were added to 25 ml of 0.3 M CaCl2 Plasmids pAAV-RC, p5E18-VD2/8 and pXR5 contain AAV genes necessary for packaging AAV2 genomes into AAV2 , AAV8 and AAV5 capsids,...
- 10
- 198
- 0
Báo cáo sinh học: "Attenuation of dengue virus infection by adeno-associated virus-mediated siRNA delivery" pdf
... therefore developed an adeno- associated virus (AAV) system capable of expressing siRNA cassettes, and tested this vector with a siRNA cassette composed of a nucleotide sequence from the 3' NCR of ... include the development of an adeno- associated virus- based siRNA approach for downregulating gene expression A recombinant AAVsiDEN3UT was utilized to induce significant decreases in DEN infection ... specific genes and loss of phenotype [15] AAV vectors have been proven to be safe and efficacious in Phase I clinical trials for gene therapy of cystic fibrosis and hemophilia B and are regarded as...
- 10
- 278
- 0
Báo cáo sinh học: " Rapid, widespread transduction of the murine myocardium using self-complementary Adeno-associated virus" pptx
... limitations For example, adenovirus has been shown to be toxic and has short-term expression[7], and lentiviral vectors stimulate inflammatory responses[8] Although adeno- associated virus has a delay ... Waele L, Iwasaki Y, Gillijns V, Wilson JM, Collen D, Chuah MK: Efficacy and safety of adeno- associated viral vectors based on serotype and vs lentiviral vectors for hemophilia B gene therapy J ... copy numbers of ssAAV and scAAV in infected hearts Serial sections of tissues used for microscopy were collected for real-time PCR analysis of AAV vector genomes Genomic DNA (gDNA) was extracted...
- 6
- 183
- 0
Báo cáo sinh học: " Application of a haematopoetic progenitor cell-targeted adeno-associated viral (AAV) vector established by selection of an AAV random peptide library on a leukaemia cell line" pot
... adenovirus and adeno- associated viral vectors: implications for gene therapy Gene Ther 2003, 10:955-963 Sun JY, nand-Jawa V, Chatterjee S, Wong KK: Immune responses to adeno- associated virus and its ... risk of those vectors [32] The safety profile of lentiviral vectors under clinical settings is still unclear Of note: although theoretically rAAV-based vectors could induce insertional mutagenesis, ... their episomal residence Thus AAV- based vectors, which have not yet been associated with any disease, offer a promising alternative Although rAAV2 vectors have been used for efficient transduction...
- 9
- 328
- 0
Báo cáo sinh học: " In vitro evaluation of a double-stranded selfcomplementary adeno-associated virus type2 vector in bone marrow stromal cells for bone healing" pptx
... PE: Adenovirus and adeno- associated virus vectors DNA Cell Biol 2002, 21:895-913 22 McCarty DM, Young SM Jr, Samulski RJ: Integration of adeno- associated virus (AAV) and recombinant AAV vectors ... Clinical gene therapy using recombinant adenoassociated virus vectors Gene Ther 2008, 15:858-863 27 Xiao X, Li J, Samulski RJ: Production of high-titer recombinant adenoassociated virus vectors ... trafficking of the vector sequence Membrane -associated heparan sulfate proteoglycan serves as the primary cell receptor for AAV type [46] This accounts for the broad host range of AAV vector Cross-packaging...
- 8
- 258
- 0
Tài liệu Báo cáo khoa học: Directed evolution of trimethoprim resistance inEscherichia coli doc
... the N-terminal end of the expressed gene This vector was used for all rounds of directed evolution When protein was purified from the pCL476 vector, although this protein was active, it failed ... constants Binding assays Binding affinity of TMP for the enzymes was measured by monitoring the quenching of tryptophan absorbance of the enzyme (ex 280 nm, em 345 nm) as increasing amounts of TMP were ... Inhibition of thymidine production is a major aspect of the activity of TMP, and inclusion of thymidine in the media Directed evolution in E coli allows bacterial growth regardless of the amount of TMP...
- 11
- 600
- 0
Báo cáo khoa học: Directed evolution of formate dehydrogenase from Candida boidinii for improved stability during entrapment in polyacrylamide ppt
... temperature of 30 °C for 30 The reaction was started by injecting 100 lmol of formate into the vial and stopped after 60 by injecting mL of HCl (1 molÆL)1) After another 30 of incubation, a gas sample of ... discrepancies of the values presented herein for wt-FDH from formerly published data is due to the use of a different analysis software: In contrast to scientist for windows, which was used by Slusarczyk ... solution was stirred for 30 The solution separated into two phases within a settling time of h The upper phase of the system was removed and mixed with PEG 6000 and H2O at final concentrations of 20%...
- 8
- 377
- 0
Báo cáo khoa học: Directed evolution of a histone acetyltransferase – enhancing thermostability, whilst maintaining catalytic activity and substrate specificity doc
... thermostable P ⁄ CAF mutants by directed evolution Second round of directed evolution To further increase the thermostability of P ⁄ CAF, a second round of evolution was performed by randomly recombining ... seven out of eight in the case of an esterase [44], three out of three for an a-glucan phosphorylase [24] and ten out of 12 for a phosphite dehydrogenase [45] Computational optimization of surface ... [20] and has ensured that directed evolution is increasingly being used to enhance the thermostability of proteins This approach involves the generation of genetic diversity in the gene encoding...
- 13
- 226
- 0
Báo cáo khoa học: Directed evolution of a glutaryl acylase into an adipyl acylase potx
... contain a b-lactamase gene, which interferes with b-lactam acylase assays Therefore, plasmid pUNN1, which contains a neomycin resistance marker, was constructed as follows: pUB110 was digested with ... mismatch oligonucleotides Ó FEBS 2002 Directed evolution of an adipyl acylase (Eur J Biochem 269) 4497 Region directed mutagenesis of the a-subunit was performed by annealing the gapped duplex ... detection was performed as described for glutaryl-7-ACA Kinetic parameters were obtained from Eadie–Hofstee plots, and the mean and standard deviation of values of at least four independent measurements...
- 10
- 447
- 0
Báo cáo sinh học: " Adeno-associated virus: from defective virus to effective vector" potx
... manipulation of AAV virions Virology 2000, 278:301-308 Grimm D, Kay MA: From virus evolution to vector revolution: use of naturally occurring serotypes of adeno- associated virus (AAV) as novel vectors for ... compartmentalization of adeno- associated virus type assembly J Virol 1997, 71:1341-1352 Ruffing M, Zentgraf H, Kleinschmidt JA: Assembly of viruslike particles by recombinant structural proteins of adeno- associated ... required for adeno- associated virus DNA replication in the absence of adenovirus coinfection J Virol 1998, 72:2777-2787 Pereira DJ, McCarty DM, Muzyczka N: The adeno- associated virus (AAV) Rep...
- 17
- 672
- 0
báo cáo hóa học:" Adeno-associated virus: from defective virus to effective vector" pdf
... manipulation of AAV virions Virology 2000, 278:301-308 Grimm D, Kay MA: From virus evolution to vector revolution: use of naturally occurring serotypes of adeno- associated virus (AAV) as novel vectors for ... compartmentalization of adeno- associated virus type assembly J Virol 1997, 71:1341-1352 Ruffing M, Zentgraf H, Kleinschmidt JA: Assembly of viruslike particles by recombinant structural proteins of adeno- associated ... required for adeno- associated virus DNA replication in the absence of adenovirus coinfection J Virol 1998, 72:2777-2787 Pereira DJ, McCarty DM, Muzyczka N: The adeno- associated virus (AAV) Rep...
- 17
- 356
- 0
Báo cáo khoa học: "Temporal and geographic evidence for evolution of Sin Nombre virus using molecular analyses of viral RNA from Colorado, New Mexico and Montana" docx
... protein Dual infections of cells with closely related hantaviruses can yield reassortant viruses (a mixture of RNA genome segments of the two viruses) and reassortant viruses have potential epidemiologic ... genetics because virus variations may reflect those of their rodent hosts, as has been suggested by Dragoo et al [4] It appears to be counterintuitive that this virus has evolved as rapidly as ... nt region of the S segment encoded 250 codons of the highly conserved nucleocapsid protein and was sampled from 21 mice This region of the S segment has not been as widely used as has the M segment...
- 16
- 264
- 0
Báo cáo khoa học: " Temporal and geographic evidence for evolution of Sin Nombre virus using molecular analyses of viral RNA from Colorado, New Mexico and Montana" docx
... protein Dual infections of cells with closely related hantaviruses can yield reassortant viruses (a mixture of RNA genome segments of the two viruses) and reassortant viruses have potential epidemiologic ... genetics because virus variations may reflect those of their rodent hosts, as has been suggested by Dragoo et al [4] It appears to be counterintuitive that this virus has evolved as rapidly as ... nt region of the S segment encoded 250 codons of the highly conserved nucleocapsid protein and was sampled from 21 mice This region of the S segment has not been as widely used as has the M segment...
- 16
- 308
- 0
Báo cáo y học: " Size does matter: overcoming the adeno-associated virus packaging limit" ppsx
... expand the utility of adeno- associated virus vectors for gene therapy Proc Natl Acad Sci USA 2000, 97:6716–6721 12 Flotte TR, Afione SA, Zeitlin PL: Adeno- associated virus vector gene expression ... virus (AAV) vectors: will they work? J Clin Invest 1994, 94:1351 Flotte TR, Carter BJ: Adeno- associated virus vectors for gene therapy Gene Ther 1995, 2:357–362 Flotte TR, Ferkol T: Genetic therapy ... Role for highly regulated rep gene expression in adeno- associated virus vector production J Virol 1997, 71: 5236–5243 Clark KR, Liu X, McGrath JP, Johnson PR: Highly purified recombinant adeno- associated...
- 3
- 169
- 0
Báo cáo y học: "Value and price of ventilator-associated pneumonia surveillance as a quality indicator" potx
... Ventilator -associated pneumonia: caveats for benchmarking Intensive Care Med 2004, 30:996-997 doi:10.1186/cc8189 Cite this article as: Aardema LM, et al.: Value and price of ventilator -associated ... Klompas M: Unintended consequences in the drive for zero Thorax 2009, 64:463-465 Klompas M: The paradox of ventilator -associated pneumonia prevention measures Crit Care 2009, 13:315 Curtis JR, Cook ... Horan TC, Andrus M, Dudeck MA: CDC/NHSN surveillance definition of health care -associated infection and criteria for specific types of infections in the acute care setting Am J Infect Control 2008,...
- 2
- 184
- 0
Tìm thêm: xác định các mục tiêu của chương trình khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25