differentiation of mouse embryonic stem cells into endothelial cells genetic selection and potential use in vivo pdf
Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... genes involved in endoderm differentiation of hESCs Table 5.1 Available information on knock-out phenotypes in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells ... clinical applications and ensuring acceptance of the transplant and preventing rejection by the recipient’s immune system remain Harnessing and understanding the differentiation potential of ... importance of Activin/ Nodal signaling in the maintenance of hESC pluripotency has been re-iterated in recent studies detailing the derivation and maintenance of induced pluripotent stem (iPS) cells...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... could maintain a small accessible region in the globin locus during the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this ... nature and extent of covalent histone modifications and association of transcription complexes at the murine b-globin locus during the in vitro differentiation of murine embryonic stem cells We ... erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time points...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted by a specific miRNA is a major obstacle in understanding the miRNA ... greater in hES cells than in EB cells and it was not even detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in ... expression profiles of miRNAs and transcripts using three hES cell lines, embryonic bodies (EB) produced from one of the cell lines and five types of terminally differentiated adult cells The integration...
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OCT 3 4 and SOX 2 are key factors for SDIA neurogenesis of mouse embryonic stem cells
... humor and inspiration, and providing invaluable thoughts on science and life, and all things in between Table of Contents Abstract Introduction Results Culturing of Mouse Embryonic Stem Cells (mESC) ... GFAP and Vimentin expressions in SDIA cultures List of Illustrations Illustration – Invitrogen BlockIT tetracycline inducible short, hairpin RNA system Introduction Mouse embryonic stem cells ... posttransfection and five days of selection were sufficient to achieve complete elimination of GFP-C2 negative control cells Mouse embryonic stem cells transfected with pcDNA6/TR and surviving Blasticidin selection...
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Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf
... We determined the H2O2-induced ROS generation in mouse -5 embryonic stem cells using DCF-DA (10 M), a fluorescent probe of intracellular H2O2 Production of ROS was -3 detected in the cells exposed ... was loaded into a 96-well plate and examined using a luminometer (Victor3; PerkinElmer, Finland) and a fluorescent plate reader with excitation and emission wavelengths of 485 nm and 535 nm, ... purpose of this study was to determine the effect of DHT on mouse ES cells under conditions of H2O2-induced oxidative stress Materials and Methods Materials The mouse ES cells were obtained from...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... expression of insulin and proinsulin in stage IV cells Quantification of IHC staining shows FN and LAM increases the percentage of (B) proinsulin, and (C) insulin positive cells (mean ± S.D.; ** indicates ... IPC differentiation Immunohistochemistry (IHC) in stage IV cells further revealed adding ECM increased the percentage of proinsulin and insulin expressing cells with the maximum effect seen in cells ... insulin and Glut2 gene expressions, proinsulin and insulin protein levels, accumulation of cytoplasmic granules including a, b, and δ granules, and insulin release in response to elevated glucose concentration...
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Báo cáo y học: "Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells" pps
... http://www.jbiomedsci.com/content/18/1/46 Page of 10 Figure Confocal microscopy of a single colony of mouse embryonic stem cells A: top view of a single slice of scanning showing most eGFP signals are not overlapping with mitochondria ... conditions in mouse embryonic stem cells Methods Mouse embryonic stem cell culture The mouse embryonic stem (MES) cells (CCE-24) were routinely grown on 0.1% gelatin-coated dishes in Dubecco’s Modified ... writing and final approval of the manuscript AK was involved in manuscript writing and data analysis SL was involved in the conception and design, administrative support, data analysis and interpretation,...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... importance of epigenetic patterns in defining cell identity, understanding and utilizing epigenomic mapping will become a necessity in both basic and translational stem cell research In this review, ... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... methylomes Studies of stringently defined models of mouse reprogramming have shown that cell-type -of- originspecific differences in gene expression and differentiation potential exist in early passage...
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Báo cáo y học: "The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells" pot
... retrotransposons (LINE and SINE B1) and rDNA repeats in wild-type OS25 ES cells and in mutant ES cells lacking Eed, Dnmt 1, Dnmt 3a/3b, G9a or Dicer The mean values and standard error of at least two independent ... maintained Here, a genetic approach was used to analyze the impact of different chromatin modifiers on the replication timing profile of mouse ES cells We show that, while early replication in ... and mutant ES cells analyzed The mean values and standard error of at least two independent experiments are shown (b,c) Summary of replication timing of candidate genes (b) and loci surrounding...
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Identification of oct4 and sox2 targets in mouse embryonic stem cells
... Genome-wide Mapping of Oct4-DNA Interactions in Mouse Embryonic Stem Cells 4.1 Introduction 74 4.2 Results 76 4.2.1 Optimisation of large-scale ChIP 76 4.2.2 Global mapping of Oct4 binding sites in mESCs ... Genome-wide Identification of Sox2-DNA Interactions in Mouse Embryonic Stem Cells 5.1 Introduction 95 5.2 Results 96 5.2.1 Optimisation of ChIP and global mapping of Sox2 binding sites in mESCs 96 5.2.2 ... Function of genes associated with the binding of Oct4, Sox2, Stat3 and Smad1 in mouse embryonic stem cells as annotated in NCBI xiii LIST OF FIGURES Figure 1.1 A schematic view of mouse pre-implantation...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... layer of mitomycin C-inactivated murine embryonic fibroblast feeder (MEF) cells [7, 8] These were harvested from CF1 inbred mouse strain purchased from Charles River Laboratories Inc (Wilmington, ... alterations 128 trained in procedures and protocols designed to minimize low temperature exposure This would include quick handling outside the incubator, as well as the use of pre-warmed and pre-equilibrated ... cannot be said of human embryos, which are much less robust and hardy compared to mouse embryos Hence laboratories involved in handling human embryos in clinical assisted reproduction often have...
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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
... osteogenic differentiation of MSCs [8,11,12] and promote fracture healing in animal models [13-15] Recombinant human BMP-2 and BMP-7 are used clinically in spinal fusion and the healing of tibial ... entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic ... basis of the expression of alkaline phosphatase (ALP) activity and incorporation of calcium in the extracellular matrix The deposition of a mineralized matrix was further examined by staining with...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... Correspondingly, in vivo implantation of BMP-4 into abdominal muscles of rats led to ectopic cartilage and bone formation when delivered as recombinant protein [38] or via genetically modified cells ... defects in humans [5,6] However, to date, the delivery of MSCs into cartilaginous lesions has neither clinically nor experimentally resulted in sustained regeneration of hyaline cartilage in vivo ... primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant adenoviral...
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Báo cáo y học: " Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development" ppt
... propagated indefinitely in vitro in the presence of leukemia inhibitory factor (LIF) [6,7] When transplanted into a mouse blastocyst, mESCs integrate into the embryo and contribute to all cell lineages, ... Maintenance of pluripotential embryonic stem cells by stem cell selection Reprod Fertil Dev 1998, 10:527-533 53 Wdziekonski B, Villageois P, Dani C: Differentiation of mouse embryonic stem cells ... steps in mESCs, we used a combination of computational and experimental approaches Analysis of the expression of TFs associated with mESC adipogenesis during mouse embryogenesis and in mouse adipose...
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Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
... osteoconductive and osteoinductive bone graft material by incorporation of Dex an osteoinductive drug into polymeric nanofibers during electrospinning process 1) Incorporation of Type I Col into nanofibrous ... labeling includes tetramethylrhodamine isothiocyanate (TRITC)-conjugated phalloidin (staining red F-actin) and DAPI (staining blue nuclei) The staining showed the formation of actin cytoskeleton in ... mesenchymal stem cells (MSCs) have been shown to be an interesting source, because they can be isolated, expanded ex vivo, and used in an autologous fashion which can avoid the problem of finding a...
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Dissecting transcriptional network in mouse embryonic stem cells
... Zfp143 is one of the genes in the list To check its expression pattern in both mouse and human ES cells and during differentiation, mouse E14 cells and human H1 cells were treated with retinoic acid ... as mouse ES cells unless stimulated by LIF and BMP4 or force expression of E-cadherin, suggesting that these cells are in a latent state of pluripotency Figure 1.1 Origin of stem cells during ... embryogenesis Indeed, a major interest of ES cells to the scientific community is their utility as cellular vehicles for engineering of the mouse genome Mouse ES cells can be injected into the blastocysts...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and anti-beta1 monoclonal antibodies Evidence that the synergy sequence of fibronectin is ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving ... colony-forming cell and the haemopoietic stem cell Blood Cells (12):7-25 32 Moore KA, Lemischka IR 2006 ;Stem cells and their niches Science (5769):1880-1885 33 Lemischka IR, Moore KA 2003 ;Stem cells: interactive...
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Baculovirus mediated genetic modification of human embryonic stem cells
... benefits of genetic manipulation include controlling differentiation of ES cells, screening and acquiring pure populations of specific types of ES cell-derived cells, altering antigenicity of cells ... recombination in human ES cells are low Electroporation, a method of choice for introducing foreign DNA into mouse ES cells, can be adapted for the gene transfection of human ES cells (Zwaka and ... embryonic stem cells for the purpose of genetic modification, thus facilitating the realization of the great potential of these cells in basic researches and clinical applications VIII LIST OF PUBLICATIONS...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... brain (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain ... counterpart of murine EC cells for studying human development and differentiation in vitro (Andrews 2002) hEC cell lines are capable of multi-lineage differentiation in vitro but being of tumor origin ... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... hFOB H & E staining (d) hFOB von Kossa staining Scaffold (e) hFOB H & E staining Figure At 2-week time point: H&E staining and von Kossa staining of sections showing scaffold and cells (e) hFOB ... comparing the in vivo osteogenic differentiation potential of human embryonic stem cells (hESC) and human somatic osteoblast cell line Method HESCs were propagated on mouse embryonic feeder cells ... be eliminated very soon Other challenges in the use of ESC include controlling lineage specific differentiation and elimination of residual stem cells Thus to obtain a purified population of osteoblasts...
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