differences between mice and men
báo cáo hóa học: " Secretory PLA2-IIA: a new inflammatory factor for Alzheimer''''s disease" pot
... MDJ, AYS, AS and GYS participated in the design and coordination of the studies and helped to draft the manuscript GYS, LL, and DGW provided the funding for the project All authors read and approved ... GACTCATGACTGTTGTTACAACC-3' and reverse 5'-TCTCAGGACTCTCTTAGGTACTA-3' that amplify a 493 bp fragment, and primers for β-actin are: forward 5'-TGGAGAAGAGCTATGAGCTGCCTG-3' and reverse 5'-GTGCCACCAGACAGCACTGTGTTG-3' ... GraphPad Prism software Significant differences between groups were assumed for P values < 0.05 Results Expression of sPLA2-IIA mRNA in hippocampus and cerebellum of AD and ND brains To demonstrate...
Ngày tải lên: 19/06/2014, 22:20
báo cáo khoa học: "Application of in situ reverse trancriptase-polymerase chain reaction (RT-PCR) to tissue microarrays" pot
... instrumentation has been designed with the aim of controlling conditions on a microscope slide, and some machines accommodate four or more slides to increase throughput and lower experimental ... described in Methods, and measured by SYBR-green staining and fluorescence quantification Results were calculated relative to a set of standards and expressed here as copy number of original transcripts ... RT-PCR methods, however, and this may be due to technical complexity and an inherent lack of reproducibility [2,3] The processing of large numbers of samples is also slow and sequential, adding...
Ngày tải lên: 11/08/2014, 00:21
Phát hiện và phân biệt chủng virus gây hội chứng rối loạn sinh sản và hô hấp ở lợn bằng phương pháp multiplex RT PCR (reverse transcription polymerase chain reaction)
... localization signal Vùng tín hiệu định vị hạch nhân Porcine Reproductive and Respiratory Syndrome Porcine Reproductive and Respiratory Syndrome Virus Hội chứng rối loạn sinh sản hô hấp lợn Virus ... chứng hô hấp sẩy thai lợn (SIRS – Swine infertility and Respiratory Syndrome), bênh sốt cao, biếng ăn, sảy thai lợn (HAAT – Hyperthermie Avortements dé Truies), bệnh tai xanh (blue ear disease) ... Invitrogen (Mỹ) Bộ hóa chất chạy PCR Fermentas (Mỹ) Cồn 70% 100% Merch (Đức) Hóa chất điện di DNA Biolabs (Mỹ) Chỉ thị DNA100bp Bộ Kit tách chiết tinh DNA từ gel Fermentas (Mỹ) Enzynomics (Korea) Invitrogen...
Ngày tải lên: 30/11/2015, 20:08
Nghiên cứu xây dựng quy trình phát hiện nhanh virus cúm gia cầm a h5n1 trong mẫu bệnh phẩm bằng kỹ thuật multiplex reverse transcription polymerase chain reaction
... (H5N1) viruses and detection of anti-H5 antibody among household and social contacts”, J Infect Dis, 180, pp 1763-70 36 Lamb RA and Krug RM (2001), “Orthomyxoviridae: The Viruses and Their Replication”, ... 344:432-438 24 Greninger A (2004), The Definition and Measurement of Dangerous Research 25 Guan Y, et al (2004), “H5N1 influenza: A protean pandemic threat”, PNAS, 101(21), pp 8156-61 26 Guan ... I, Pyhala R, Hovi T, 1985, Enzyme immunoassay, complement fixation and hemagglutination inhibition tests in the diagnosis of influenza A and B virus infections”, Purified hemagglutinin in subtype-specific...
Ngày tải lên: 10/02/2014, 20:39
Báo cáo khoa học: "TaqMan reverse transcription polymerase chain reaction for the detection of Japanese encephalitis virus" pot
... sensitivity and linear relationship between copy number and cycle threshold (Ct) values The technique uses oligonucleotide probes labeled with fluorescent dyes, a reporter at the 5' end and a quencher ... fetuses of pigs Materials and Methods Viruses and sample preparation The JEV isolate KV1899, Anyang 300 (attenuated vaccine strain) and Nakayama strain were used as standard virus for detection ... or 50 cycles of 10 s at 95oC to denature and 20 s at 55oC to anneal and 20 s at 72oC for extension and at 72oC for final extension Reverse transcription and PCR amplification were performed by...
Ngày tải lên: 07/08/2014, 18:20
Báo cáo khoa học: "Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus" pps
... 55/lizarb/oriezurc/42A rof ecneuqes ehT sniarts 21 eht rof dezylana saw noiger tegrat ehT )ASU ,ratsanD( erawtfos ratsanD eht gnisu dengila dna )1 elbaT( A C aisA 2TAS O O O O O O O O 55/lizarB/oriezurC/42A ... eht gnitarapes ybereht ,esaremylop AND qaT fo ytivitca esaelcunoxe eht ot eud dedarged si eborp eht ,srucco noitacifilpma sA tegrat RCP eht nihtiw ecneuqes AND dnarts-elgnis yratnemelpmoc sti...
Ngày tải lên: 07/08/2014, 18:21
Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
... mechanism, no correlation between CD4 and CD8 cell counts and mRNA levels of CD4 and CD8 could be determined by QC-RT-PCR There was also no correlation between CD4 and CD8 mRNA levels and the number of ... Buffer (Gibco BRL, Grand Island, NY), 0.25 µg of each primer, mM MgC12 in each CD4 reaction and mM MgC12 for CD8 reactions, mM total dNTP's, and 0.5 U of Taq Polymerase For CD4 and CD8 reaction conditions ... cell ratios and are therefore likely to be HIV infected (the majority of the samples sent for flow cytometry are HIV-positive) The relationship between the mRNA and cell number for CD4 and CD8 are...
Ngày tải lên: 11/08/2014, 08:20
Xây dựng qui trình phát hiện đồng thời yeallow head virus(YHV), taura syndrome virus(TSV) và gill associated virus(GAV) trên tôm bằng kỹ thuật multiplex reverse transcription polymerase chain reaction
... gây chết tôm thẻ chân trắng phát lần khu vực cửa sông Taura tỉnh Guayas, Ecuador, nhà khoa học Jimenez công bố, sau lây lan khắp lãnh thổ nước Nạn nhân dịch bệnh nước Peru, Columbia, Honduras, ... Ecuador Virus GAV phát Úc liên quan đến phức hợp bệnh đầu vàng Virus tồn cục quần thể tôm sú Queensland - Úc, Malaysia Thái Lan Lây nhiễm tự nhiên với GAV tìm thấy tôm Penaeus monodon, lây nhiễm thực ... reverse transcriptase) Nhưng enzyme phải có mồi cho phản ứng reverse Chúng dùng mồi ngẫu nhiên (random hexamer primers) mà không dùng mồi đặc hiệu để tổng hợp toàn RNA thành cDNA Sau sử dụng cặp...
Ngày tải lên: 31/08/2014, 07:43
Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx
... the experiments and wrote the manuscript SC conceived the studies and participated in experimental design and coordination All authors read and approved the final manuscript Acknowledgements The ... improved[15] Complementary DNA and RNA probes have been used to detect RNA and mRNA of the CDV genome with improved specificity and sensitivity[6,7] Primers P1 and P4 (specific for CDV and conserved ... fragment only for the wild-type strain, while P3/P4, P5/ P6 generated a same 177 bp fragment only for the vaccine strain, and both fragments could be amplified from the mixture of CDV vaccine and...
Ngày tải lên: 12/08/2014, 04:20
Báo cáo hóa học: "Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real-time reverse transcriptase-polymerase chain reaction to predict recurrence of gastric adenocarcinoma" potx
... of the study FA and WDY drafted the manuscript and participated in the statistical analysis HP and XRH conceived of the study, and participated in its design and coordination and helped to draft ... aberrations [5,6] and that they grow ex vivo as cell lines with a malignant phenotype [7] Several approaches to detect CTCs have been described and can be classified into PCR-based methods and cytometric ... transferred into a 30mL falcon tube and centrifuged at 1,800 rpm at room temperature for 20 minutes Serum was removed, and cells were resuspended in mL saline and 0.3 mL RNA later solution After...
Ngày tải lên: 18/06/2014, 16:20
Báo cáo y học: "Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction" potx
... -2, and -3 in the synovium synthase-1, 2, and -3 and (OA) levels of the messages (RA) Relative expressionand rheumatoid arthritis for hyaluronanof knees in 2, and -3 and hyaluronidase-1, -2, and ... knees and the relative differences in message levels between the control group and patients with OA or RA were determined by realtime RT-PCR in accordance with the manufacturer's instructions and ... mechanism and is digested into 20-kDa fragments by hyaluronidase-2 located in vesicles at an acidic pH; subsequently, the fragments are transported into lysosomes, where hyaluronidase-1 and two...
Ngày tải lên: 09/08/2014, 01:24
Báo cáo y học: " A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses?" docx
... cowrote and edited the manuscript YL, H L, FL, XC, TS and GC took samples and did laboratory testing QZ and YY organized the overall project and helped edit the manuscript All the authors read and ... WEEV and EEEV detection at the same time The primers and probes were selected from E3 gene for WEEV and NSP3 gene for EEEV, and the sequence analysis confirmed the specificity for WEEV and EEEV, ... assay and Taqman RT-PCR assay for WEEV and EEEV [11] But none of the above methods was available for detecting and distinguishing WEEV and EEEV in a single reaction In this study, we developed and...
Ngày tải lên: 12/08/2014, 01:22
Báo cáo y học: " A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control" docx
... planned the experimental design and drafted the manuscript JL conceived the study, participated in its design and coordination, and helped to revise the manuscript All authors read and approved the ... >45 30.11 >45 32.14 >45 36.12 >45 Concentrations of HCV RNA and armored RNA were indicated by FAM and Cy5 signals, respectively Meng and Li Virology Journal 2010, 7:117 http://www.virologyj.com/content/7/1/117 ... duplex primer/probe A and B reaction system Meng and Li Virology Journal 2010, 7:117 http://www.virologyj.com/content/7/1/117 Page of Table 4: Testing results of different assays and kits for 109...
Ngày tải lên: 12/08/2014, 04:20
Báo cáo y học: "Multiplex Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS-PCR) for diagnosis of natural infection with canine distemper virus" potx
... contemporary vaccine strain, was freestanding and located between our local isolates and the CDV strains isolated in America This contrasted with Vacc-N and Vacc-Q, which were clustered in the ... spacer between the M and F genes and part of the F gene, was initially synthesised from the cDNAs of the seven CDV field isolates and the three commercial vaccines using the primer set CDF-F and ... manufacturer's instructions Total RNA (1 μg) and random 8-mer primers (50 μM) were denatured at 65°C for and cooled down on ice To synthesise the first-strand cDNA, the RNA and primers were mixed in × reaction...
Ngày tải lên: 12/08/2014, 04:20
Báo cáo khoa học: " An analysis of the subtypes of dengue fever infections in Barbados 2003–2007 by reverse transcriptase polymerase chain reaction" pptx
... have been seen in our population and this could be similar in other Caribbean islands Methods Specimen collection One hundred and seventy-two whole blood specimens collected during the period ... 108 cases of DHF and cases of DSS, with a total of deaths Large outbreaks of dengue occurred in Barbados in 1995 and 1997 and were associated with circulation of serotype (1995) and serotype (1997), ... 45 specimens were analysed of which 14 were males and 31 were females There were 22, 22, 22 and 61 patients selected from 2004–2007 respectively (Table 3) This gave a total of 71 males and 101...
Ngày tải lên: 12/08/2014, 04:21
Báo cáo thú y: " DNA methylation patterns detected by the Amplified Methylation Polymorphism Polymerase Chain Reaction (AMP PCR) technique among various cell types of bulls" pot
... clear and reproducible marker bands were counted, and the comparison of bands between genomic and digested templates was done according to the appearance of the marker mentioned above Number and ... fibroblasts and the others While Bull No exhibited significant differences in all markers: R marker between leukocytes and fibroblasts, S marker between leukocytes and the others and D marker between ... the experiment DH and BC contributed in the experimental designs and techniques MO and MT provided the concept of the experiment and helped to draft the manuscript All authors read and approved...
Ngày tải lên: 12/08/2014, 18:22
Nghiên cứu xây dựng quy trình phát hiện nhanh virus cúm gia cầm A H5N1 trong mẫu bệnh phẩm bằng kỹ thuật multiplex reverse transcription polymerase chain reacti
... RNA virus sinh phẩm QIAamp viral RNA Mini Handbook for purification of viral RNA - Hóa chất phát virus cúm A/H5N1 sinh phẩm QIAGEN OneStep RT-PCR Kit Handbook - Hoá chất tạo plastmid tái tổ hợp ... plasmid pJET1.2/blunt (Fermentas) tạo plasmid tái tổ hợp, sau đó tiế n hành tổ ng hơ ̣p RNA với bô ̣ sinh phẩ m TranscriptAid™ T High Yield Transcription Kit(Fermentas) Nồ ng đô ̣ RNA đươ ... ngăn chặn lây lan dịch bệnh người Các biện pháp phòng ngừa WHO CDC (Centers for Disease Control and Prevention) đưa nhằm làm giảm tỉ lệ mắc cúm gia cầm người cách thực biện pháp bảo vệ cá nhân...
Ngày tải lên: 31/03/2015, 16:19
Recombinant DNA I - Basics of molecular cloning Polymerase chain reaction cDNA clones and screening
... into bacteria • Lambda phage and P1 phage can carry large fragments of DNA – 20 kb for lambda – 70 to 300 kb for P1 • M13 phage vectors can be used to generate single-stranded DNA YAC vectors for ... the DNA fragment between the primers increases about 2-fold with each cycle • For n = number of cycles, the amplification is approximately [2exp(n-1)]-2 • After 21 cycles, the fragment has been ... specific DNA segments • Polymerase Chain Reaction • Requires knowledge of the DNA sequence in the region of interest • As more sequence information becomes available, the uses of PCR expand • With...
Ngày tải lên: 13/03/2014, 18:39
ỨNG DỤNG PHƯƠNG PHÁP PCR (POLYMERASE CHAIN REACTION) VÀ PHƯƠNG PHÁP NUÔI CẤY ĐỂ KHẢO SÁT SỰ NHIỄM VI SINH VẬT GÂY BỆNH TRONG THỰC PHẨM ĐƯỜNG PHỐ
... Proskauer WHO : World Health Organization XLD : Xylose Lysine Desoxycholate ISO : International Standards Organization EDTA : Ethylene Diamine Tetraacetic Acid DANH MỤC CÁC BẢNG Bảng 1.1 Triệu chứng ... 0/250ml 10 102 E coli/g KQĐ 0 S aureus/g KQĐ 10 Clostridium 0/250ml 10 Salmonella/25g KQĐ KQĐ Nấm men, mốc/g KQĐ 10 KQĐ Chỉ tiêu thử nghiệm Tổng vi khuẩn hiếu khí/g Coliforms/g perfringens/g Nguồn: ... lí nhiệt Vi khuẩn hiếu khí/g 104 10 Coliforms/g 10 E coli/g 0 S aureus/g 0 Salmonella/25g 0 Nấm men, mốc/g 10 10 Chỉ tiêu thử nghiệm Nguồn: [2] 1.1.5.3 Tiêu chuẩn vệ sinh an tồn thực phẩm kem,...
Ngày tải lên: 16/03/2013, 09:26
KỸ THUẬT PCR (POLYMERASE CHAIN REACTION)
... (phản ứng chuỗi trùng hợp - Polymerase Chain Reaction) phương pháp in vitro để nhân nhanh đoạn AND đó, có độ nhạy cao, mà cần khối lượng mẫu ban đầu hạn chế II Nguyên lý Kỹ thuật tổng hợp ADN ... nhiên kỹ thuật PCR có khác dùng nhiệt độ cao (94 oC) tháo xoắn thay cho helicase kết hợp với enzym AND polymerase chịu nhiệt hệ thống điều nhiệt thích hợp cho giai đoạn phản ứng tổng hợp với đoạn...
Ngày tải lên: 24/04/2013, 15:41