detection of chlamydia trachomatis in genitourinary specimens by using the roche amplicor pcr kit
Báo cáo khoa học: "Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR" pot
... present in the porcine intestine and feces including swine genomic DNA The number of amplification cycles was one of the important factors for increasing the sensitivity of the PCR assay The increase ... determine the accuracy of the PCR assay for the detection of L intracellularis infection (Table 1) Mucosal scraping and fecal samples were obtained from each pig Of 17 mucosal scrapings, 16 specimens ... methods include H E staining and silver staining of the intestinal sections 36 Dong-Kyun Suh et al Fig Section of the ileum from a pig infected with L intracellularis a : Presence of numerous intracellular,...
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Báo cáo khoa học: " Detection of Babesia divergens in southern Norway by using an immunofluorescence antibody test in cow sera" docx
... possible cause of the decline in incidence since the 1930 s is changes in the use of pastures Changes in legislation leading to increased use of wood pasturing may reverse the decline Hasle et ... redwater in only ten of the farms where B divergens was detected, and only four of these cases of redwater had been recorded by the NDHRS (Figure 3) All of the cows on one of the farms in the study ... indicating repeated infections during the season This implies that the lifetime risk of acquiring bovine babesiosis is higher than the current rate of infection that was determined in the study...
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Báo cáo y học: "Detection of Chlamydia trachomatis-DNA in synovial fluid: evaluation of the sensitivity of different DNA extraction methods and amplification systems" pps
... with the same amplification system The same detection limits were observed using alkaline lysis in combination with the plasmid PCR and the 16s RNA PCR as well as using the Qiagen tissue kit in ... persisting Chlamydia are showing an Altogether, alkaline lysis and Qiaex II gel extraction kit + CTAB gave reproducibly the highest detection rates in the C trachomatis EB as well as in the C trachomatis ... mind be considered the most feasible detection system of C trachomatis in SF for standardized testing in a clinical practice and to advance the diagnosis of CIA 10 11 12 Competing interests The...
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Báo cáo y học: "Effective detection of rare variants in pooled DNA samples using Cross-pool tailcurve analysis" ppsx
... present in only a single pool) Cross-pool filtering using SERVIC4E Further validation by Sanger sequencing indicated the persistence of a few false positive calls from this dataset Analysis of these ... defined as the ratio of the sum of Phred quality scores of the variant base call to the sum of Phred quality scores of all base calls; (3) average quality, defined as the average quality of all ... limited error modeling The pattern of errors observed in many libraries may be dependent on the sequencecontext of the reads, the preparation of the library being sequenced, the sequencing chemistry...
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Báo cáo y học: "Continuous noninvasive monitoring of barbiturate coma in critically ill children using the Bispectral™ index monitor" doc
... awake) In addition, the EEG recorded by the BIS is continuously displayed (BIS-EEG), together with the device's estimate of the SR The SR calculated by the BIS (SR-BIS) represents the percentage of ... Guidelines for the acute medical management of severe traumatic brain injury in infants, children, and adolescents Chapter 13 The use of barbiturates in the control of intracranial hypertension in ... of the study and in the interpretation of the data JHB participated in the interpretation of the data and helped to draft the manuscript DT conceived the study and participated in its design GHV...
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Báo cáo khoa học: "Time of initial detection of fetal and extra-fetal structures by ultrasonographic examination in Miniature Schnauzer bitches" ppsx
... minA llamS J stnemerusaem latef cihpargonosartlu gnisu sehctib ezis llams dna muidem ni ega lanoitatseg fo noitanimreteD A inoirG ,CG inovuL 31 23-72 ,14 ,6002 minA tsemoD dorpeR ycnangerp eninac ... -103 ,8 ,7691 tcarP minA llamS J ygolohprom lacigolotyc dna euqinhceT I ygolotyc lanigav eninaC PA ettuhcS 71 04-53 392 ,33 ,2991 dnuosartlU loidaR teV suretu mutraptsop eninac eht fo yhpargoidar ... serutcurts latef fo noitceted laitini fo emit ehT )82 yaD ,1 giF( oyrbme eht dessapmocne hcihw ,)92-62( 0.1 ± 7.72 yad no detceted saw ,enarbmem cinoinma eht ,enarbmem latef cinegohce ssel ,dnoces A )72-62(...
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Báo cáo y học: " Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood" doc
... values suggesting less virus and lower sensitivity, and no differences in the timing of the collections or in the age or weight of the children In any case, the detection rates of the two collection ... and the CT values were similar in the samples collected by the pediatrician and parents Furthermore, the direct involvement of parents significantly increased the patient's acceptance of the ... losing the sample when respiratory episodes occur Secondly, the samples can be obtained immediately after the onset of the first signs and symptoms, thus favouring the identification of the infectious...
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Báo cáo khoa học: " A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification" pps
... a finding confirmed by our results In other words, the specificity and the detection limit of the LAMP assay are equal with FQPCR for detecting GPV The optimal condition for detecting GPV by ... survey on GPV infection in goose farming In doing so, the virus-carrying goslings can be identified during the early stages of infection; countermeasures can be devised even before the infection ... Inc.) to the tube, and by observing the color of the solution The solution changed from light orange to green in the presence of LAMP amplicons, while it remained light orange in the absence of amplification...
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Báo cáo y học: "Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model" pptx
... protein was synthesized by a multistep PCR using pCR2 /rβ2M as a template The first PCR was performed to add the L2 sequence at the 3' end of β2m gene The following or steps of PCRs were performed ... against the virus in a mouse system in vivo [30] Thus, it will also be necessary to assess the in vivo effect of the rat SCTs for the evaluation of the system as a therapeutic tool in HTLV-I infection ... HTLV-I infected cells, as well assessment of the effects of immune T cells on the development of the disease [17,18] By using this model system, we also reported the therapeutic effect of Tax-coding...
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Tài liệu Báo cáo khoa học: "Automatic Detection of Nonreferential It in Spoken Multi-Party Dialog" doc
... positives The slightly higher recall of the model using the sentence boundary information is mainly due to a better coverage of the rule using the features encoding the distance to the next toinfinitive ... performed the actual annotation again The results reported in the following are from this second annotation We then examined the inter-annotator reliability of the annotation by calculating the κ ... encode, among other things, information about the parts of speech and lemmata of words in the context of it (obtained automatically) Other features encode the presence or absence of, resp the distance...
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Báo cáo " Development of climate change scenarios for small areas in Vietnam by using the MAGICC/SCENGEN software in combination with statistic correction" docx
... consideration In the study, the temperature and rainfall scenarios are built in the monthly form of decades of the 21st century However, within the scope of this paper, we only introduce climate ... value in increasing level, followed by Quy Nhon and Can Tho This is quite compatible with the temperature happening in the past (increasing level declined southwards) In addition, temperatures in ... (B2) By the end of the 21st century, under the emission scenarios from medium to high, annual rainfall can increase about 5-9% in Da Nang, 2– 4% in Quy Nhon and 2% in Can Tho In dry season, the...
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Detection of Pseudomonas aeruginosa in sputum headspace through volatile organic compound analysis potx
... Steinheim, Germany) were injected using the same GC-MS method to determine the retention indices of the individual compounds using a modified Kovats method [33] Bacterial culturing Sputa were inoculated ... significant higher in vivo concentrations in most strains of P aeruginosa [18] This biomarker could then be used in the detection of P aeruginosa in breath, whether or not in combination with CH3SCN ... identify the major source of a certain VOC We tried to minimize the effect of saliva contamination by asking the patient to rinse their mouth prior to sputum production Nonetheless, finding a biomarker...
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R"eCseoarcuh garthicl eofficer screening" improves detection of pulmonary tuberculosis in hospital in-patients pptx
... admitted to the ICU and paediatrics departments after completion of Stage I By excluding the TB patients in the ICU, we found that 81% and 100% of TB patients in the Internal Medicine Department in Stage ... difference might due to the increased awareness of TB in Stage II (Figure 2), or because of interference of TB diagnosis by other severe symptoms among patients in the ICU during Stage I In fact, our COS ... (17 in the internal medicine department, in the surgical department, and 21 in the ICU) in stage I, and 12 patients (all in the internal medicine department) in stage II The times from admission...
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Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot
... previously In the pIFNHA construct, the IFN-b gene was tagged by PCR using an oligonucleotide containing three repetitions of the sequence corresponding to the human in uenza A virus hemaglutinin (HA) ... (Fig 1A) We then analysed, by Northern blot, the induction and decay of IFN-b transcript in the same conditions As shown in Fig 1B, IFN-b mRNA was detectable h after the beginning of the infection, ... [13], further supports the involvement of this protein in the deadenylation process of IFN-b mRNA Interestingly, IFN-b ARE does not recruit other ARE-binding factors (data not shown), thereby emphasizing...
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ag doped wo3-based powder sensor for the detection of no gas in air
... when the temperature of the Ag catalyst is increased to 250 8C, the sensitivity of the WO3 sensor kept at 150 8C 71 increased by three times Without heating the Ag catalyst or bypassing the gas ... patterns (the lack of their long-range order and their heterogeneity may explain the poor re¯ection hump obtained in XRD) Typically, as seen in the ®gure, a region containing ca Ê 6.93 A inter-planar ... gases) containing ca 100 ppm NO balanced with air) by the ac impedance analyzer (Fig 1) The ¯ow rate of the gases was kept at 100 ml/min throughout the measurement using mass ¯ow controllers The schematic...
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Báo cáo " On the detection of gross errors in digital terrain model source data " pdf
... Those parameters are: ‐ The size of the moving window, i.e. the number and location of neighbor points. ‐ The interpolation technique used for estimating height of the considered points. ... inside the moving window; coefficient (4) j K V takes a value in the range from 2 to 4. j =1 where mi is the number of data points that fall inside the moving window around point ... radius (or of the minimum number of points inside the search window), the number of correctly and incorrectly detected points is decreasing. This can be explained as a ...
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DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID-SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/MASS SPECTROMETRY pdf
... portion of the sample into the solvent reservoir The water sample will drain into the cartridge, and from the exit into the suction flask Maintain the packing material in the cartridge immersed in ... (MeCl2) by adding the solvent to the disk, drawing about half through the disk, allowing it to soak the disk for about a minute, then drawing the remaining solvent through the disk Note: Soaking the ... methanol (MeOH) by adding the MeOH to the disk and allowing it to soak for about a minute, then drawing most of the remaining MeOH through A layer of MeOH must be left on the surface of the disk, which...
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The bleach method improves the detection of pulmonary tuberculosis in Laos ppt
... staining was performed: carbol fuchsin 0.3%, slow heating until steaming, rinsing after min, destaining with 25% sulfuric acid, rinsing, counterstaining with methylene blue 0.3% for min, rinsing ... from the health centres: the risk of contamination by sample handling and the long delays between sputum collection and smear staining The addition of 5% NaOCl to the container disinfects the ... compare the sensitivity and the specificity of the two methods Furthermore, the prevalence of HIV co-infection among the patients enrolled was too small to assess the benefits of the method in people...
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Báo cáo khoa học: Translation initiation region dependency of translation initiation in Escherichia coli by IF1 and kasugamycin pdf
... fMet-tRNA binding to the P-site [10,12,13], probably by stabilizing IF2 binding to the 30S subunit [14], and stimulates the GTPase activity of IF2 [15] It also increases binding of mRNA to the initiation ... IF1 mutant strains by the inactivation of cspA is another functional link to IF1 In view of the finding that IF1 has RNA chaperone activity [26], the effects of the elimination of BipA, YggJ and ... model It distorts the P-site, thereby disturbing the start codon position and preventing fMet-tRNA binding during the stage of 50S subunit joining [32,33] Several aspects of kasugamycin action are...
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origin of coherent phonons in bi2te3 excited by ultrafast laser pulses
... the thickness is 10 nm [Fig 4(b)] We verified that the thinner films still have crystalline structure, as shown in the Raman scattering data in Fig 4(c) The widths of the Raman peaks in the thinner ... are introduced to the molecular system by slightly displacing the atomic positions along the directions indicated in Fig For example, the A1 phonon 1g mode is generated in MD by stretching the ... film illuminated by 30◦ incident pump beam is determined to be 2636 K It is then found from Eq (2) that in our case, the thermal force fthermal = 1.42 × 1016 N/m3 dominates at the end of pump pulse,...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25