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detection of bacteria in liver tissue from patients with hepatocellular carcinoma using laser capture microdissection technique lcm

Báo cáo y học:

Báo cáo y học: "Characterization of erythrovirus B19 genomes isolated in liver tissues from patients with fulminant hepatitis and biliary atresia who underwent liver transplantation"

Y học thưởng thức

... difference with a P value of less than 0.05 was considered significant Results B19 DNA in liver tissues was detected in 10 of 28 (35.7%) patients with fulminant hepatitis and of 19 (36.8%) patients with ... study to obtain direct evidence of a viral sequence in the liver from patients who underwent liver transplantation In this retrospective study of 47 patients with liver diseases of unknown etiology, ... diseases including liver diseases in different countries is now planning and we shall report on further investigations in the future In conclusion, we found a high prevalence of B19 infection in livers...
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Báo cáo y học:

Báo cáo y học: " CD8+ T lymphocytes in lung tissue from patients with idiopathic pulmonary fibrosis" pot

Báo cáo khoa học

... have included a mixed population of patients with UIP/IPF, patients with non-specific interstitial pneumonia and patients with pulmonary fibrosis associated with collagen vascular disorders since ... form of fibrosing interstitial pneumonia, the non-specific interstitial pneumonia (NSIP) [42] Indeed, studies that compared the prognosis of patients with "idiopathic" NSIP to that of patients with ... (% of total cells) and MRC dyspnea score, (rs = 0.63, p = 0.02) most prominent and disabling symptom in these patients This study shows that the type of the inflammatory infiltrate in lung tissue...
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Isolation and detection of bacteria in soil, ash and sediment with the contamination of paraquat and glyphosate

Isolation and detection of bacteria in soil, ash and sediment with the contamination of paraquat and glyphosate

Tổng hợp

... density of each type of bacteria in Ash – PRQherbicide mixture 32 Table 4.5 The density of each type of bacteria in Ash - GPTherbicide mixture 32 Table 4.6 The density of bacteria in herbicide ... 4.1 Herbicide mixtures using in this experiment 26 Table 4.2 The C.F.U of bacteria per 1mL in each type of herbicide mixtures 27 Table 4.3 Number of bacteria detected in six herbicide mixtures ... laboratories and clinical techniques, include DNA cloning for sequencing, detection of bacteria or viruses (particularly AIDS), functional analysis of genes; the diagnosis of 16 hereditary diseases...
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Detection of bacteria and enteric viruses from river and estuarine sediment

Detection of bacteria and enteric viruses from river and estuarine sediment

Môi trường

... directly from 0.5 g wet sediment samples using ISOIL for Beads Beating (Nippon Gene, Tokyo) Detection of pathogens and fecal indicators by PCR and qPCR methods The concentration of (c)DNA of Poliovirus ... Adenovirus was determined using real-time qPCR methods with LightCycler ST300 (Roche, Tokyo) Each 20 The PCR condition including a denaturing step at 95 oC for 10 min, followed by 50 cycles of 95 oC for ... high prevalence of these fecal indicators suggested that part of the fecal indicator bacteria that flew into the river from various contamination sources settle on river and estuarine sediment,...
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Báo cáo y học:

Báo cáo y học: "Higher susceptibility to Fas ligand induced apoptosis and altered α modulation of cell death by tumor necrosis factor-α in periarticular tenocytes from patients with knee joint osteoarthritis" ppt

Báo cáo khoa học

... examination of tendon tissue from patients with knee joint osteoarthritis (OA) and controls (a) Haematoxylin and eosin staining of tendons from OA patients revealed characteristic changes of tendon ... presence of degenerative changes in the tendon samples of patients with knee joint OA but not in the controls (Fig 1a) We found partial disruption of tissue structure, with fibrillations and inhomogeneous ... expression of Fas in all tissue samples investigated There was scattered staining for Fas throughout the tissues (Fig 1c,d) Although it appeared that the staining was more intense in the OA samples,...
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Báo cáo khoa học:

Báo cáo khoa học: "Differentiating the impact of anatomic and non-anatomic liver resection on early recurrence in patients with Hepatocellular Carcinoma" pps

Báo cáo khoa học

... groups in terms of tumor staging Vascular invasion (T3) occurred in patients in the anatomic group (32.1%) and patients in the non-anatomic group (28%) Nodal involvement occurred in patients in the ... after liver resection in patients with solitary hepatocellular carcinoma J Hepatobiliary Pancreat Surg 2005, 12:399-404 Suh KS: Systematic hepatectomy for small hepatocellular carcinoma in Korea ... differentiated T1: single tumor < cm without vascular invasion, T2: single tumor > cm without vascular invasion, T3: solitary tumor >2 cm with vascular invasion, T4: tumor involving a major branch...
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Báo cáo y học:

Báo cáo y học: "Osteogenic protein 1 in synovial fluid from patients with rheumatoid arthritis or osteoarthritis: relationship with disease and levels of hyaluronan and antigenic keratan sulfate" ppt

Báo cáo khoa học

... measurement of OP-1 in joint fluid may prove to be of potential value in the clinical evaluation of joint disease processes The results also suggest that identification of the binding partners of OP1 in ... clarified Another interesting finding is that OP-1 was present in the cleaved active form in the SF of OA patients and RA patients, suggesting that, at least in part, the active form of OP-1 could ... for delivery into the joints Because OP-1 has been identified in all connective tissues of the synovial joint, the OP1 found in SF could be derived from any or all of these tissues; this remains...
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Báo cáo khoa hoc:

Báo cáo khoa hoc:" No evidence of enhanced oxidant production in blood obtained from patients with obstructive sleep apnea" potx

Báo cáo khoa học

... sifying the quality of our results in showing no priming or activation of blood phagocytes in untreated OSAS patients The sympathetic over activity in OSAS patients resulting in the rise of plasma ... significant suppression of fMLP- induced respiratory burst of isolated PMNs was reported in patients with severe OSAS using the chemiluminescence technique [16-18] Taking into account the divergences ... episodes during sleep did not change the morning and evening intensity of the resting as well as fMLP-induced LBCL, FRAP and blood H2O2 activities in the OSAS patients These suggest a lack of apparent...
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Báo cáo y học:

Báo cáo y học: "Safety of rFVIIa in hemodynamically unstable polytrauma patients with traumatic brain injury: post hoc analysis of 30 patients from a prospective, randomized, placebo-controlled, double-blind clinical trial" pps

Báo cáo khoa học

... when using rFVIIa in trauma patients Thus, TE events occurred in 4% (6 of 138) of the placebo-treated patients as compared with 4% (6 of 139) of the rFVIIa-treated patients The incidence of fatal ... severity of brain injury None of the 81 trauma patients in this series had any clinical indication of TE events Zaaroor and Bar-Lavie [23] reported the first series of five patients with TBI with ... axonal injury, one ischemia, one edema, one intraventricular hemorrhage, and two unspecified), occurring in the remaining 10 of 30 (33%) patients with TBI Thirteen (43%) of the patients with TBI...
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Báo cáo y học:

Báo cáo y học: "Phenotypic and functional characterization of switch memory B cells from patients with oligoarticular juvenile idiopathic arthritis" pps

Báo cáo khoa học

... Characterization of B-cell infiltrates in synovial tissue from JIA patients In three JIA cases (one with oligoarticular persistent and two with oligoarticular extended JIA), histologic analysis of synovial tissue ... reported [13], by using purified goat antihuman IgG or IgA or IgM from Southern Biotechnology Associates (Birmingham, AL, USA) After washing and blocking with PBS containing 1% BSA for 30 minutes, serial ... later In all flow-cytometry experiments performed in this study, the first step was the exclusion of non-B cells stained with a combination of the following antibodies and detected in a single...
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Báo cáo y học:

Báo cáo y học: " Weight and metabolic effects of cpap in obstructive sleep apnea patients with obesity" pot

Báo cáo khoa học

... for changes in weight, hormone levels and sleep parameters [r(pvalue)] HOMAIR BMI HOMAIR Ghrelin Leptin Insulin Adipon ectin Resistin Ghrelin Leptin Insulin Adiponectin nectin Resistin ESS CPAP ... http://respiratory-research.com/content/12/1/80 in its regulation including changes in insulin sensitivity Insulin administration has been shown to suppress circulating ghrelin levels in some [31] but not all studies [32] Plasma insulin ... levels and insulin resistance correlate inversely with ghrelin This association was BMIindependent in some studies [33] However in a study using euglycemic hyperinsulinemic clamp method, insulin sensitivity...
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Báo cáo khoa học:

Báo cáo khoa học: "Offset of pharmacodynamic effects and safety of remifentanil in intensive care unit patients with various degrees of renal impairment" ppt

Báo cáo khoa học

... conducted to assess the offset times of the pharmacodynamic effects of prolonged remifentanil infusions in ICU patients with varying degrees of renal impairment, including patients with severe renal ... safety profiles of remifentanil administered by continuous intravenous infusion for up to 72 hours in ICU patients with varying degrees of renal impairment The study was conducted in accordance with ... conducted within 12 hours before the start of remifentanil administration, together with measurement of plasma and urinary creatinine concentration, using the following equation: CLcr (ml/min) =...
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Báo cáo y học:

Báo cáo y học: "Macrophage migration inhibitory factor in cerebrospinal fluid from patients with central nervous system infection" potx

Báo cáo khoa học

... higher in patients with purulent meningitis and encephalitis than in patients with lymphocytic meningitis and patients suspected of having meningitis but without evidence of CNS infection In accordance ... biochemical parameters in CSF and blood are summarized in Table In patients with purulent meningitis of known aetiology, in patients with lymphocytic meningitis and in patients with encephalitis, ... was initiated before the lumbar puncture in 23% (7/31) of patients with purulent meningitis of known bacterial aetiology and in 15% (3/20) of those with an unknown bacterial aetiology The initial...
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Báo cáo y học:

Báo cáo y học: "Determinants of mortality in non-neutropenic ICU patients with candidaemia" pps

Báo cáo khoa học

... Ethics Committees of all participating institutions Informed written consent was obtained from patients that were included in the study Definitions The definition of ICU acquisition of candidaemia ... subset of patients in whom fluconazole was the sole initial antifungal therapy during the initial 72 hours of therapy (but exclusion of patients with breakthrough infection, defined as occurrence of ... Antifungal therapy was initiated in 156 (85%) patients: of these, fluconazole in 76%, amphotericin B deoxycholate in 12%, a lipid formulation of amphotericin B in 4%, caspofungin in 4%, and voriconazole...
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báo cáo khoa học:

báo cáo khoa học: "Analysis of the recurrence risk factors for the patients with hepatocellular carcinoma meeting University of California San Francisco criteria after curative hepatectomy" pot

Báo cáo khoa học

... numbers of points from each factor were cumulated to produce a total number of points for that patient A vertical line is then drawn from the line indicating the total number of points to the line indicating ... Recurrence within 5years 583cases Group B No recurrence within 5years 257cases Figure Outcome overview of patients with resectable primary hepatocellular carcinoma (HCC) within UCSF criteria (PT-INR), ... for the patients with tumors within UCSF criteria which were superior to those of the patients with tumors beyond UCSF criteria (Figure 2) However, these treatment results of the patients with tumors...
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Quantification of Microcystin-degrading Bacteria in a Biofilm from a Practical Biological Treatment Facility by Real-time PCR

Quantification of Microcystin-degrading Bacteria in a Biofilm from a Practical Biological Treatment Facility by Real-time PCR

Môi trường

... The number of cells increased in fall, and the number of microcystin-degrading bacteria increased with the increase in the concentration of phytoplankton By using methods described in this paper, ... 0.005% of the total bacteria present in the biofilm, and their cell number increased during fall (Fig 3) Fig - Seasonal changes in the abundance ratio of microcystin-degrading bacteria in the biofilm ... slight increase for the Y2 strain) (Fig 1B) A B Fig - (A) Comparison of detection of mlrA gene using QMF and QMR (B) Comparison of detection of mlrA gene using MF and MR primers Arrows indicate...
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Báo cáo sinh học:

Báo cáo sinh học: "Detailed genetic analysis of hemagglutinin-neuraminidase glycoprotein gene in human parainfluenza virus type 1 isolates from patients with acute respiratory infection between 2002 and 2009 in Yamagata prefecture, Japa" docx

Hóa học - Dầu khí

... tree consisting of dated tips by using the nucleotide sequences of the HPIV1 HN coding region of 43 isolated strains from patients in Yamagata prefecture and reference strains by using the ML ... genetic analysis of hemagglutininneuraminidase glycoprotein gene in human parainfluenza virus type isolates from patients with acute respiratory infection between 2002 and 2009 in Yamagata prefecture, ... first report of these findings in HPIV1 The distribution of amino acids affects the structure of the HN coding region in HPIV1, and previous reports show that substitutions of amino acids in HN glycoprotein...
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Báo cáo sinh học:

Báo cáo sinh học: " Transmission of human hepatitis C virus from patients in secondary cells for long term culture" pot

Điện - Điện tử

... analysis of HCV proteins binding to IgG from patient sera Dot blot analysis of HCV proteins binding to IgG from patient sera HCV proteins were obtained from tissue culture fluid Protein preparations ... participants included 108 males and 48 females All specimens were freshly processed within an hour of blood drawing Repeat samples were obtained from 77 of the original patients in order to confirm our initial ... contaminating proteins (B) Analysis of low molecular weight proteins of CIMM-HCV cultured in various cell lines Lane total protein from HCV positive sera, lane transformed B-cells infected with...
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