derivation and characterization of neural cells from embryonic stem cells using nestin enhancer pdf
Báo cáo Y học: Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod pdf
... N-Terminal sequencing of the kininogens was only successful from the 42-kDa band of wolffish kininogen (XLVQPGVLI…, Table 1) The major bands of both kininogens and also the 60-kDa band of cod kininogen ... metalloproteinase-1 derived from human fibroblasts and from HT-1080 fibrosarcoma cells Eur J Biochem 259, 892–840 Laemmli, U.K (1970) Cleavage of structural proteins during assembly of the head of bacteriophage ... kininogen and 6200 UÆmg)1 for wolffish kininogen (units are defined Fig SDS/PAGE visualized by silver staining of purified cod kininogen and wolf fish kininogen, and MALDI-TOF mass spectra of kininogens...
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Báo cáo khoa học: " Isolation and characterization of Streptococcus sp.from diseased flounder (Paralichthys olivaceus )in Jeju Island" ppt
... eht fo esahp suoeuqa reppu eht no detcelloc saw AND lairetcaB nim rof 000,6 ta degufirtnec dna nim rof sllec lairetcab gniliob yb detcartxe neht saw AND lairetcab ;retaw dellitsid elbuod dezilirets ... 817( rof evitisop ,9~4 senal ;)pb 001,1( rof evitisop ,3 dna senal ;noitcefni laccocotperts rof evitagen ,1 enal ;lortnoc evitagen ,N enal ;)pb 003 ,M4040 ( lortnoc evitisop ,P senal ;reddal AND ... enilav ,esapil rof snoitcaer evitagen dah setalosi llA nispyrtomyhc -α dna esatahpsohp enilakla rof snoitcaer evitisop dah fo setalosi 81 dna esadinimasoculg-β-lyteca -N dna esaretse rof snoitcaer...
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báo cáo khoa học: " Cloning and characterization of a glucosyltransferase from Crocus sativus stigmas involved in flavonoid glucosylation" pdf
... to analyse and identify the 5' and 3' ends of the glucosyltransferases For these, μg of poly(A)+ RNA from stigmas was used to synthesize the 5' and 3' ends of the first-strand cDNA using Superscript ... tissue of of C niveus, C speciosus and C cancellatus suggests a role of CsGT45 in the accumulation of specific kaempferol glucosides in the stigma of Crocus species Unaltered Expression of CsGT45 ... (+1da), and three days after anthesis (+3da), and in closed and open stamen (stc and sto), corm, tepals (pt) and style Equal amounts of total RNA were used in each reaction The levels of the constitutively...
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Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection" pdf
... and mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and growth ... x 105 Figure cells with an HIV-EGFP vector at a moi = 0.5 Infection of parental and mutant HeLa cells (30-2 and 42-7) Infection of parental and mutant HeLa cells (30-2 and 42-7) cells with an ... infection of a wide variety of cells) have revealed differences in the efficiency of single round infection in cells of differing types and species [11,12] Therefore, to build upon and extend...
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Identification and characterization of novel anticoagulants from bungarus fasciatus venom
... activity of pooled fractions Chapter Three Figure 3.1: Purification of BF-AC1 and BF-AC2 from the venom of B fasciatus Figure 3.2: ESI-MS profile of BF-AC1 (A) and BF-AC2 (B) Figure 3.3: Structural characterization ... in the hind limbs of experimental animals through acting on the Na+ channel of plasmatic membrane of skeletal muscle cells to induce depolarization of these cells and influx of Na+ [42] Several ... Kang Tse Siang, Professor R Manjunatha Kini and Associate Professor Go Mei Lin for their constant encouragement and scientific input throughout my candidature Dr Kang and Prof Kini have provided...
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Identification and characterization of novel proteins from a rare australian elapid snake drysdalia coronoides
... 41 Materials and Methods 42 iii Reagents and kits; Collection of venom and venom gland; RNA isolation and cDNA synthesis; Cloning of ds cDNA; Isolation of plasmids and verification of clones; cDNA ... goes to our collaborator Prof Daniel Bertrand from Department of Neuroscience, University of Geneva, Geneva, Switzerland for carrying out a part of pharmacological studies of drysdalin in his laboratory ... 2.13 Schematic showing the summary of characterization of novel proteins from D coronoides 78 ix Chapter 3.1 Overview of pET systems 81 3.2 Comparison of optimized and native sequences encoding drysdalin...
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Isolation and characterization of anticoagulant protein from the venom of hemachatus haemachatus (african ringhals cobra
... (SEC) of Hemachatus haemachatus crude venom using a Superdex 30 column Cation exchange of peak from SEC RP-HPLC profiles of hemextin A and hemextin B Rechromatography of hemextin A and B ESI-MS of ... proteins Aim and scope of the thesis 60 Chapter Two Purification of the Anticoagulant Protein Isolation and Purification of hemextin A and hemextin B Assessment of homogeneity of hemextin A and hemextin ... form of zymogen FVII Schematic representation of the approaches for TF-FVIIa inhibition Ribbon diagrams of the second and third kuniz domain of TFPI, Mechanism of action of TFPI, Ribbon diagram of...
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Isolation and characterization of cellulose nanofibrils from wheat straw
... The images were loaded into the software and diameters of the fibers were measured using a two point measuring analysis The scale of the software was calibrated using the scale bars on each TEM ... in Tables and Figure shows the phase images of AFM of cellulose nanofibrils at different magnifications Figure 1a and b are phase images and Figure 1c and d are topographical images of the nanofibers ... nanofibers from the cell wall and TEM images reveal the separation of these nanofibers from the microsizes fiber bundles The diameter of the fibers is found in the range of 10–60 nm A tendency of agglomeration...
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Báo cáo hóa học: "Synthesis and characterization of VO2-based thermochromic thin films for energy-efficient windows" pdf
... derivative of both curves of the hysteresis loops (heating and cooling) and considering the mean value Results and discussion Structural characterization The crystal structure of the three sets of films ... Mo, and Nb and different doping concentrations We report on the influence of each element and respective concentrations on the crystal structure of the films, optical/thermochromic performance and ... multilayer films of VO2 and TiO2 with enhanced transmittance Solar Energy Mater Solar Cells 2009, 93:1685 Jin P, Xu G, Tazawa M, Yoshimura K: Design, formation and characterization of a novel multifunctional...
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Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx
... Reactivity of NCT C-terminal antibody after SDS/PAGE for PS1-deficient mouse neural stem cells, PS1 wild-type neural cells, E16 mouse brain and adult mouse brain showed three major bands of 110, 120 and ... mouse- and human-derived membrane preparations were examined for PEN-2 immunoreactivity and revealed strong detection of a 10-kDa band in PS1+/ +neural stem cells, embryonic brain, and SY5Y cells; ... Membranes from tissues and cells were analysed for PEN-2 (1–15) reactivity Significant expression was detected as a 10-kDa band in PS1 wild-type neural stem cells, E15 mouse brain and SY5Y cells Low-level...
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Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
... diameter of the PVDF membrane, the PVDF-PS membrane and the PVDF-SPS membrane is 0.25 µm, 0.12 µm and 0.16 µm, respectively It can be seen that the polymerization of styrene and the blending of PS and ... tissue paper The weight of the membrane was measured using an electronic balance immediately The stability of the membrane was evaluated using the reduction of its weight Results and discussion 3.1 ... in our laboratory using a combination technique of thermally induced polymerization and phase inversion, and all of the other reagents were analytical grade 2.2 Preparation of the PVDF-based...
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Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf
... BMP-R-Smad ortholog from S mansoni Fig Structure of the SmSmad1B gene, cDNA and protein (A) Schematic representations of the SmSmad1B gene, cDNA and protein and the amino acid sequence of SmSmad1B protein ... properties of SmSmad1 and SmSmad1B as described above However, in the presence of SmSmad4 and the active mutant form of TbRI, both SmSmad2 and SmSmad4 interacted with GCN5, indicating the formation of ... 4087 SmSmad1B, a BMP-R-Smad ortholog from S mansoni J M Carlo et al enhanced the interaction of SmSmad2 and SmSmad4 and resulted in the formation of a stable and functional Smad complex [6,7] The...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... correspond to any of the four B-chains presented here Purification of four pulchellin isoforms from A pulchellus seeds Using a combination of affinity, ion exchange and chromatofocusing chromatography, ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5.2 for the four isoforms respectively Secondary structure of the pulchellin isoforms ... III ⁄ P IV and a mixture of other isoforms (*), respectively, in the presence (lanes 2–5) or absence (lanes 6–9) of 2-mercaptoethanol (C) Elution profile of P III and P IV Chromatofocusing chromatography...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... Da) The identity of this peptide was then confirmed by MALDI-TOF ⁄ TOF fragment ion analysis, where partial sequences of this peptide were obtained from the ladders of b-fragment and y-fragment ... digestion of Coomassie Brilliant blue-stained protein bands from SDS ⁄ PAGE was performed as described by Bamford et al [22] MS MALDI-TOF MS was performed using an Ultraflex TOF ⁄ TOF instrument ... Isolation and characterization of the tyrosinase gene from Neurospora crassa J Biol Chem 264, 17250– 17258 27 van Gelder CW, Flurkey WH & Wichers HJ (1997) Sequence and structural features of plant and...
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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt
... Figure 7A, D, I and L are diamidino-2phenylindole hydrochloride (DAPI) stained cells showing the nucleus, and Fig 7C, F, K and N are the merged images of A and B, D and E, I and J, and L and M, respectively ... stresses used for treatment of pea seedlings were cold (A and B), salinity (C and D), wounding (E and F), drought (G and H), SA (I and J), ABA (K and L) and calcium (N and O) Panel M is the control ... Isolation and sequence analysis of PsCIPK and CBL cDNAs and genomic clones For cDNA cloning, first partial fragments of 550 bp for PsCIPK and 335 bp for PsCBL were amplified by PCR using double-stranded...
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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
... (from residue number 3) to the sequence of the corresponding component of PH from A calcoaceticus NCIB 8250 (11/11 identity) [3] and very similar to that of the corresponding component of PH from ... samples Spectra were recorded from 260 to 190 nm at a speed of 50 nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation ... of 0.6 lM The data were fitted to Michaelis–Menten curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of...
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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt
... absence of boiling (Fig 1A, lanes and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of ... Figure 1A shows the SDS/PAGE of purified ccNiR upon different treatments The complex dissociates into an intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its ... 2003 Characterization of ccNiR subunits (Eur J Biochem 270) 3905 rather than four covalently bound c-type hemes [12] The resolution of the 3D structural of NrfA isolated from S deleyianum [14] and...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... O-acetylsialic acid and agglutination ability of the De-O-acetylated preparation of glycoproteins De-Oacetylation of glycoproteins was performed following the procedures of Sarris and Palade [30] and Schauer ... roseus): purification and characterization Nippon Suisan Gokkaishi 48, 1427 48 Kamiya, H., Muramoto, K & Goto, R (1987) Isolation and characterization of agglutinins from the hemolymph of acorn barnacle, ... microtiter plates and mixed with 25 lL of a 1.5% suspension of erythrocytes obtained from various mammalian species The HA titer was determined as the reciprocal of the highest dilution of serum giving...
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Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Purification and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc
... urease-negative, and catalase- and oxidase-positive It oxidatively produced a small amount of acid from D-glucose, D-fructose and sucrose Alkali was produced from L-asparagine, citrate, galactarate and tartrate ... determining the values of Km and Vmax, used the double reciprocal of the Michaelis–Menten equation The Ki value was obtained using different concentrations (1.6, 3.2 and 4.8 lM) of 4-aminoresorcinol ... All steps of the enzyme purification were carried out at 0–4 °C All centrifugations were at 20 000 g and °C for 10 A wet weight of 30 g of Bordetella sp strain 10d cells were obtained from a 4.8-L...
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Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf
... maximum of the a band at 557 nm absorption maxima at 420 nm (c band), 530 nm (b band) and 557 nm (a band) are characteristic of cytochrome b Heme was extracted from the protein with acidic acetone and ... downstream of dsrA, and in dsrB, dsrH, and dsrM, lead to an inability of the cells to oxidize intracellular sulfur to sulfite under photolithoautotrophic conditions The ability of the mutant cells ... enzymes of sulfate reduction, namely adenosine 5¢-phosphosulfate reductase and sulfite reductase Here we report on the isolation and characterization of a heme-containing membrane protein from A...
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